Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multifunctional tryptophan biosynthesis protein

Gene

trpC

Organism
Aspergillus awamori (Black koji mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (trpC)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (trpC)
  4. Multifunctional tryptophan biosynthesis protein (trpC)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Nucleophile; for GATase activityBy similarity1
Binding sitei106GlutamineBy similarity1
Active sitei197For GATase activityBy similarity1
Active sitei199For GATase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Isomerase, Lyase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040
UPA00035; UER00042
UPA00035; UER00043

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trpC
OrganismiAspergillus awamori (Black koji mold)
Taxonomic identifieri105351 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568541 – 768Multifunctional tryptophan biosynthesis proteinAdd BLAST768

Proteomic databases

PRIDEiP18483

Structurei

3D structure databases

ProteinModelPortaliP18483
SMRiP18483
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 223Glutamine amidotransferase type-1Add BLAST199

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 76Glutamine bindingBy similarity3
Regioni152 – 153Glutamine bindingBy similarity2
Regioni253 – 517Indole-3-glycerol phosphate synthaseAdd BLAST265
Regioni533 – 768N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST236

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

CDDicd00331 IGPS, 1 hit
cd00405 PRAI, 1 hit
Gene3Di3.20.20.70, 3 hits
3.40.50.880, 1 hit
HAMAPiMF_00135 PRAI, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR016302 Anthranilate_synth_II
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR013798 Indole-3-glycerol_P_synth
IPR001468 Indole-3-GlycerolPSynthase_CS
IPR001240 PRAI
IPR011060 RibuloseP-bd_barrel
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00218 IGPS, 1 hit
PF00697 PRAI, 1 hit
PIRSFiPIRSF001382 TrpG-trpC-trpF, 1 hit
SUPFAMiSSF51366 SSF51366, 3 hits
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS00614 IGPS, 1 hit

Sequencei

Sequence statusi: Complete.

P18483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSGLVDHS PHHPTKAAQL NTASNVILID NYDSFTWNVY LVLEGATVNV
60 70 80 90 100
FRNDQITLEE LIAKKPTQLV ISPGPGHPET DAGISSAAIQ YFSGKIPIFG
110 120 130 140 150
VCMGQQCIIT CFGGKVDVTG EILHGKTSAL KHDGKGAYEG LPDSLALTRY
160 170 180 190 200
HSLAGTHATI PDCLEVSSSV QLTDDSNKDV IMGVRHKKLA VEGVQFHPES
210 220 230 240 250
ILTEYGRTMF RNFLKLTAGT WEGNGKHFDE QSNTTKATVS SNTAPKTDKK
260 270 280 290 300
LSILERIYDH RRAAVAVQKT IPSQRPADLQ AAYDLNLAPP QVPFPARLRQ
310 320 330 340 350
SPYPLSLMAE IKRASPSKGM IAENACAPAQ ARQYAKAGAS VISVLTEPEW
360 370 380 390 400
FKGSIDDLRA VRQSLEGLTN RPAILRKEFV FDEYQILEAR LAGADTVLLI
410 420 430 440 450
VKMLSVELLT RLYHYSRSLG MEPLVEVNTP EEMKIAVDLG AEVIGVNNRD
460 470 480 490 500
LTSFEVDLGT TSRLMDQVPS STIVCALSGI SGPKDVEAYK KEGVKAILVG
510 520 530 540 550
EALMRAADTA AFIAELLGGS SQNVSKESRS SPLVKICGTR SEEAARAAIE
560 570 580 590 600
AGADLIGIIM VQGRTRCVPD DVALRISQVV KSIPKPAGQT PPTSRGTPAA
610 620 630 640 650
ASVEYFDHSA RILRHPSRAL LVGVFQNQPL DYILSQQQKL GLDVVQLHGS
660 670 680 690 700
EPLEWAKLIP VPVIRKFGLD EPAIARRAYH SLPLLDSGVG GTGELLDQSR
710 720 730 740 750
VQNVLDKDSG LRVILAGGLD PTNVAGIVQK LGESGRKVVG VDVSSGVESD
760
GAQDLGKIRA FVQAVRGL
Length:768
Mass (Da):82,657
Last modified:November 1, 1990 - v1
Checksum:iE3C0DB1AB10225CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53576 Genomic DNA Translation: CAA37640.1
PIRiS11161

Similar proteinsi

Entry informationi

Entry nameiTRPG_ASPAW
AccessioniPrimary (citable) accession number: P18483
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health