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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trpC

Organism
Aspergillus awamori (Black koji mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathway:iL-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (trpC)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (trpC)
  4. Multifunctional tryptophan biosynthesis protein (trpC)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021For GATase activityBy similarity
Active sitei197 – 1971For GATase activityBy similarity
Active sitei199 – 1991For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trpC
OrganismiAspergillus awamori (Black koji mold)
Taxonomic identifieri105351 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768Multifunctional tryptophan biosynthesis proteinPRO_0000056854Add
BLAST

Proteomic databases

PRIDEiP18483.

Structurei

3D structure databases

ProteinModelPortaliP18483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 223199Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni253 – 517265Indole-3-glycerol phosphate synthaseAdd
BLAST
Regioni533 – 768236N-(5'-phosphoribosyl)anthranilate isomeraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSGLVDHS PHHPTKAAQL NTASNVILID NYDSFTWNVY LVLEGATVNV
60 70 80 90 100
FRNDQITLEE LIAKKPTQLV ISPGPGHPET DAGISSAAIQ YFSGKIPIFG
110 120 130 140 150
VCMGQQCIIT CFGGKVDVTG EILHGKTSAL KHDGKGAYEG LPDSLALTRY
160 170 180 190 200
HSLAGTHATI PDCLEVSSSV QLTDDSNKDV IMGVRHKKLA VEGVQFHPES
210 220 230 240 250
ILTEYGRTMF RNFLKLTAGT WEGNGKHFDE QSNTTKATVS SNTAPKTDKK
260 270 280 290 300
LSILERIYDH RRAAVAVQKT IPSQRPADLQ AAYDLNLAPP QVPFPARLRQ
310 320 330 340 350
SPYPLSLMAE IKRASPSKGM IAENACAPAQ ARQYAKAGAS VISVLTEPEW
360 370 380 390 400
FKGSIDDLRA VRQSLEGLTN RPAILRKEFV FDEYQILEAR LAGADTVLLI
410 420 430 440 450
VKMLSVELLT RLYHYSRSLG MEPLVEVNTP EEMKIAVDLG AEVIGVNNRD
460 470 480 490 500
LTSFEVDLGT TSRLMDQVPS STIVCALSGI SGPKDVEAYK KEGVKAILVG
510 520 530 540 550
EALMRAADTA AFIAELLGGS SQNVSKESRS SPLVKICGTR SEEAARAAIE
560 570 580 590 600
AGADLIGIIM VQGRTRCVPD DVALRISQVV KSIPKPAGQT PPTSRGTPAA
610 620 630 640 650
ASVEYFDHSA RILRHPSRAL LVGVFQNQPL DYILSQQQKL GLDVVQLHGS
660 670 680 690 700
EPLEWAKLIP VPVIRKFGLD EPAIARRAYH SLPLLDSGVG GTGELLDQSR
710 720 730 740 750
VQNVLDKDSG LRVILAGGLD PTNVAGIVQK LGESGRKVVG VDVSSGVESD
760
GAQDLGKIRA FVQAVRGL
Length:768
Mass (Da):82,657
Last modified:November 1, 1990 - v1
Checksum:iE3C0DB1AB10225CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53576 Genomic DNA. Translation: CAA37640.1.
PIRiS11161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53576 Genomic DNA. Translation: CAA37640.1.
PIRiS11161.

3D structure databases

ProteinModelPortaliP18483.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP18483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3Di3.20.20.70. 3 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genomic sequence encoding trpC from Aspergillus niger var. awamori."
    Adams R.R., Royer T.
    Nucleic Acids Res. 18:4931-4931(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 22342 / NRRL 3112.

Entry informationi

Entry nameiTRPG_ASPAW
AccessioniPrimary (citable) accession number: P18483
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 1, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.