P18480 (SNF5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SWI/SNF chromatin-remodeling complex subunit SNF5

Alternative name(s):
SWI/SNF complex subunit SNF5
Transcription factor TYE4
Transcription regulatory protein SNF5

Gene names

Name:	SNF5
Synonyms:	SWI10, TYE4
Ordered Locus Names:	YBR289W
ORF Names:	YBR2036

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in transcriptional activation. Component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.
Subunit structure	Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 217 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SNF5 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon catabolite activation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 14580348. Source: SGD chromatin remodeling Inferred from mutant phenotype PubMed 1459453. Source: SGD double-strand break repair via homologous recombination Inferred from mutant phenotype PubMed 16024655. Source: SGD sucrose catabolic process Inferred from mutant phenotype PubMed 6392017. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	SWI/SNF complex Inferred from direct assay PubMed 18644858PubMed 8016655PubMed 8127913PubMed 8159677. Source: SGD nuclear chromosome Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA polymerase II activating transcription factor binding Inferred from mutant phenotype PubMed 14580348. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TAF14	P35189	2	EBI-17546,EBI-18920

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	SWI/SNF chromatin-remodeling complex subunit SNF5		PRO_0000205955
Regions
	Compositional bias	31 – 270	240	Gln-rich
	Compositional bias	72 – 132	61	Pro-rich
	Compositional bias	272 – 324	53	Pro-rich
	Compositional bias	489 – 588	100	Asp/Glu-rich (acidic)
	Compositional bias	714 – 882	169	Pro-rich
	Compositional bias	755 – 798	44	Arg/Lys-rich (basic)
Amino acid modifications
	Modified residue	818	1	Phosphoserine Ref.7 Ref.8
	Modified residue	821	1	Phosphoserine Ref.8
	Modified residue	851	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	564	1	D → E in CAA53652. Ref.2
	Sequence conflict	564	1	D → E in CAA85254. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P18480 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: F6D31A57E6D6DA94	FASTA	905	102,543
	10 20 30 40 50 60 MNNQPQGTNS VPNSIGNIFS NIGTPSFNMA QIPQQLYQSL TPQQLQMIQQ RHQQLLRSRL 70 80 90 100 110 120 QQQQQQQQQT SPPPQTHQSP PPPPQQSQPI ANQSATSTPP PPPAPHNLHP QIGQVPLAPA 130 140 150 160 170 180 PINLPPQIAQ LPLATQQQVL NKLRQQAIAK NNPQVVNAIT VAQQQVQRQI EQQKGQQTAQ 190 200 210 220 230 240 TQLEQQRQLL VQQQQQQQLR NQIQRQQQQQ FRHHVQIQQQ QQKQQQQQQQ HQQQQQQQQQ 250 260 270 280 290 300 QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQGQ IPQSQQVPQV RSMSGQPPTN VQPTIGQLPQ 310 320 330 340 350 360 LPKLNLPKYQ TIQYDPPETK LPYPTYWSDK KADTDTLLYE QIIQRDKINK YSLIRETNGY 370 380 390 400 410 420 DPFSIYGFSN KEYISRLWHT LKYYQDLKNT RMKSITSTSQ KIPSASIWGN GYSGYGNGIT 430 440 450 460 470 480 NTTTRVIPQV EVGNRKHYLE DKLKVYKQAM NETSEQLVPI RLEFDQDRDR FFLRDTLLWN 490 500 510 520 530 540 KNDKLIKIED FVDDMLRDYR FEDATREQHI DTICQSIQEQ IQEFQGNPYI ELNQDRLGGD 550 560 570 580 590 600 DLRIRIKLDI VVGQNQLIDQ FEWDISNSDN CPEEFAESMC QELELPGEFV TAIAHSIREQ 610 620 630 640 650 660 VHMYHKSLAL LGYNFDGSAI EDDDIRSRML PTITLDDVYR PAAESKIFTP NLLQISAAEL 670 680 690 700 710 720 ERLDKDKDRD TRRKRRQGRS NRRGMLALSG TSASNTSMNG VHNTVAAGNA SSLPPGEILL 730 740 750 760 770 780 PDIADIPRTF RTPVPSTLMP GGVDVGPSVE SYELRNTTTY KSRPDRPKPV SPPCYIIDHI 790 800 810 820 830 840 PGHSLLLSIK LPGKVNTKEE FAAAPNDTSS GTNAMLPSPE SLKTKLNSNI RAGVTIPSIP 850 860 870 880 890 900 NPIANHTVTN SPNPTLQPVI PGGAASKSVP TPSLPIAPPV APHDSEATLL TNSNNGSSNN NTQNT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes." Laurent B.C., Treitel M.A., Carlson M. Mol. Cell. Biol. 10:5616-5625(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MCY.
[2]	"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Holmstroem K., Brandt T., Kallesoe T. Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 564. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-818, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-818; SER-821 AND SER-851, MASS SPECTROMETRY.
[9]	"Structural analysis of the yeast SWI/SNF chromatin remodeling complex." Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L., Peterson C.L. Nat. Struct. Biol. 10:141-145(2003) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, ELECTRON MICROSCOPY OF THE SWI/SNF COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36482 Genomic DNA. Translation: AAA35062.1. X76053 Genomic DNA. Translation: CAA53652.1. Z36158 Genomic DNA. Translation: CAA85254.1. BK006936 Genomic DNA. Translation: DAA07404.2.
PIR	RGBYS5. S44551.
RefSeq	NP_009848.4. NM_001178637.4.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2364N.
IntAct	P18480. 44 interactions.
MINT	MINT-424303.
STRING	4932.YBR289W.
Proteomic databases
PaxDb	P18480.
PeptideAtlas	P18480.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR289W; YBR289W; YBR289W.
GeneID	852592.
KEGG	sce:YBR289W. sce:YBR294W.
Organism-specific databases
CYGD	YBR289w.
SGD	S000000493. SNF5.
Phylogenomic databases
eggNOG	NOG291299.
GeneTree	ENSGT00700000104696.
KO	K11648. K14708.
OMA	IREQVHM.
OrthoDB	EOG4J9R74.
Gene expression databases
Genevestigator	P18480.
GermOnline	YBR289W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006939. SNF5. [Graphical view]
PANTHER	PTHR10019. PTHR10019. 1 hit.
Pfam	PF04855. SNF5. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971757.

Entry information

Entry name

SNF5_YEAST

Accession

Primary (citable) accession number: P18480
Secondary accession number(s): D6VQT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program