P18475 (TORSO_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase receptor torso
EC=2.7.10.1

Gene names

Name:	tor
ORF Names:	CG1389

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable receptor with tyrosine-protein kinase activity. Required for determination of anterior and posterior terminal structures in the Drosophila embryo. The ligand of torso seems to be TSL. Ref.1 Ref.5
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed throughout the embryo but is activated specifically at the poles. Ref.1 Ref.5
Developmental stage	Expressed both maternally and zygotically. Ref.1 Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Malpighian tubule morphogenesis Traceable author statement PubMed 10512197. Source: FlyBase cell fate determination Traceable author statement PubMed 9631645. Source: FlyBase chorion-containing eggshell pattern formation Inferred from mutant phenotype PubMed 1783295. Source: FlyBase gastrulation Inferred from mutant phenotype PubMed 14602078. Source: FlyBase metamorphosis Inferred from mutant phenotype PubMed 19965758. Source: FlyBase pole cell migration Inferred from mutant phenotype PubMed 14602078. Source: FlyBase protein autophosphorylation Inferred from direct assay Ref.5. Source: FlyBase terminal region determination Inferred from mutant phenotype PubMed 10652276Ref.1. Source: FlyBase torso signaling pathway Traceable author statement PubMed 10494038. Source: FlyBase
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Non-traceable author statement PubMed 10512197. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane receptor protein tyrosine kinase activity Inferred from sequence or structural similarity Ref.1. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P18475-1)

Also known as: C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P18475-2)

The sequence of this isoform differs from the canonical sequence as follows:
     907-923: KVEQVPCEEELYLEPLN → IGLRISAK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 923	903	Tyrosine-protein kinase receptor torso		PRO_0000024477
Regions
	Topological domain	21 – 399	379	Extracellular Potential
	Transmembrane	400 – 420	21	Helical; Potential
	Topological domain	421 – 923	503	Cytoplasmic Potential
	Domain	475 – 874	400	Protein kinase
	Nucleotide binding	481 – 489	9	ATP By similarity
Sites
	Active site	741	1	Proton acceptor By similarity
	Binding site	502	1	ATP By similarity
Amino acid modifications
	Modified residue	608	1	Phosphoserine Ref.6
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	146	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	314	1	N-linked (GlcNAc...) Potential
	Glycosylation	326	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential
	Glycosylation	377	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	907 – 923	17	KVEQV…LEPLN → IGLRISAK in isoform B.		VSP_034011
Experimental info
	Sequence conflict	125	1	A → T in CAA33247. Ref.1
	Sequence conflict	387	1	H → P in CAA33247. Ref.1
	Sequence conflict	667	1	R → Q in CAA33247. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (C) [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 746F46E1A4277ACF	FASTA	923	105,201
	10 20 30 40 50 60 MLIFYAKYAF IFWFFVGSNQ GEMLLMDKIS HDKTLLNVTA CTQNCLEKGQ MDFRSCLKDC 70 80 90 100 110 120 RINGTFPGAL RKVQENYQMN MICRTESEIV FQIDWVQHSR GTEPAPNATY IIRVDAVKDD 130 140 150 160 170 180 NKETALYLSD DNFLILPGLE SNSTHNITAL AMHGDGSYSL IAKDQTFATL IRGYQPSKMG 190 200 210 220 230 240 AVNLLRFVPQ PDDLHHIAAE IEWKPSAESN CYFDMVSYST NSVNMDEPLE VQFRDRKKLY 250 260 270 280 290 300 RHTVDNLEFD KQYHVGVRTV NIMNRLESDL QWLPIAVPSC LDWYPYNYTL CPPHKPENLT 310 320 330 340 350 360 VTQKQYLPNI LALNITWARP RYLPDNYTLH IFDLFKGGTE LNYTLDQNRS HFYVPKITVL 370 380 390 400 410 420 GSHFEVHLVA QSAGGKNVSG LTLDKVHRGV LLSEGNMVKL VLFIIVPICC ILMLCSLTFC 430 440 450 460 470 480 RRNRSEVQAL QMDAKDAKAS EFHLSLMDSS GLLVTLSANE SLEVMDELEV EPHSVLLQDV 490 500 510 520 530 540 LGEGAFGLVR RGVYKKRQVA VKLLKDEPND EDVYAFKCEI QMLKAVGKHP NIVGIVGYST 550 560 570 580 590 600 RFSNQMMLLI EYCSLGSLQN FLREEWKFRQ EQNAIGLKKN LEQNVDNRRF NRLPRNSIHD 610 620 630 640 650 660 RIEDINNSML STVEEESESD QTHSSRCETY TLTRITNAAD NKGYGLEDIE NIGGSYIPKT 670 680 690 700 710 720 AEAPKDRPKR KLKPQPKKDS KQDFKSDNKK RIFENKEYFD CLDSSDTKPR IPLKYADLLD 730 740 750 760 770 780 IAQQVAVGME FLAQNKVVHR DLAARNVLIS VDRSIKIADF GLSRDVYHEN VYRKSGGSGK 790 800 810 820 830 840 LPIKWLALES LTHQVYTSQS DVWSFGVLLY EITTLGGMPY PSVSPSDLLQ LLRQGHRMKR 850 860 870 880 890 900 PEGCTQEMFS LMESCWSSVP SHRPTFSALK HRLGGMILAT NDVPERLKQL QAATESKLKS 910 920 CDGLNSKVEQ VPCEEELYLE PLN
	Isoform B [UniParc]. Checksum: E56B6FB1098131DB	FASTA	914	104,026
	10 20 30 40 50 60 MLIFYAKYAF IFWFFVGSNQ GEMLLMDKIS HDKTLLNVTA CTQNCLEKGQ MDFRSCLKDC 70 80 90 100 110 120 RINGTFPGAL RKVQENYQMN MICRTESEIV FQIDWVQHSR GTEPAPNATY IIRVDAVKDD 130 140 150 160 170 180 NKETALYLSD DNFLILPGLE SNSTHNITAL AMHGDGSYSL IAKDQTFATL IRGYQPSKMG 190 200 210 220 230 240 AVNLLRFVPQ PDDLHHIAAE IEWKPSAESN CYFDMVSYST NSVNMDEPLE VQFRDRKKLY 250 260 270 280 290 300 RHTVDNLEFD KQYHVGVRTV NIMNRLESDL QWLPIAVPSC LDWYPYNYTL CPPHKPENLT 310 320 330 340 350 360 VTQKQYLPNI LALNITWARP RYLPDNYTLH IFDLFKGGTE LNYTLDQNRS HFYVPKITVL 370 380 390 400 410 420 GSHFEVHLVA QSAGGKNVSG LTLDKVHRGV LLSEGNMVKL VLFIIVPICC ILMLCSLTFC 430 440 450 460 470 480 RRNRSEVQAL QMDAKDAKAS EFHLSLMDSS GLLVTLSANE SLEVMDELEV EPHSVLLQDV 490 500 510 520 530 540 LGEGAFGLVR RGVYKKRQVA VKLLKDEPND EDVYAFKCEI QMLKAVGKHP NIVGIVGYST 550 560 570 580 590 600 RFSNQMMLLI EYCSLGSLQN FLREEWKFRQ EQNAIGLKKN LEQNVDNRRF NRLPRNSIHD 610 620 630 640 650 660 RIEDINNSML STVEEESESD QTHSSRCETY TLTRITNAAD NKGYGLEDIE NIGGSYIPKT 670 680 690 700 710 720 AEAPKDRPKR KLKPQPKKDS KQDFKSDNKK RIFENKEYFD CLDSSDTKPR IPLKYADLLD 730 740 750 760 770 780 IAQQVAVGME FLAQNKVVHR DLAARNVLIS VDRSIKIADF GLSRDVYHEN VYRKSGGSGK 790 800 810 820 830 840 LPIKWLALES LTHQVYTSQS DVWSFGVLLY EITTLGGMPY PSVSPSDLLQ LLRQGHRMKR 850 860 870 880 890 900 PEGCTQEMFS LMESCWSSVP SHRPTFSALK HRLGGMILAT NDVPERLKQL QAATESKLKS 910 CDGLNSIGLR ISAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila gene torso encodes a putative receptor tyrosine kinase." Sprenger F., Stevens L.M., Nuesslein-Volhard C. Nature 338:478-483(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[5]	"Biochemical analysis of torso and D-raf during Drosophila embryogenesis: implications for terminal signal transduction." Sprenger F., Torsoclair M.M., Morrison D.K. Mol. Cell. Biol. 13:1163-1172(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-608, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15150 Genomic DNA. Translation: CAA33247.1. AE013599 Genomic DNA. Translation: AAF59203.1. AE013599 Genomic DNA. Translation: ABV53713.1. AE013599 Genomic DNA. Translation: ABV53714.1. AY071403 mRNA. Translation: AAL49025.1.
PIR	S03900.
RefSeq	NP_001097211.1. NM_001103741.1. NP_001097212.1. NM_001103742.1. NP_476762.1. NM_057414.3.
UniGene	Dm.512.
3D structure databases
ProteinModelPortal	P18475.
SMR	P18475. Positions 462-890.
ModBase	Search...
Protein-protein interaction databases
IntAct	P18475. 1 interaction.
MINT	MINT-755149.
Proteomic databases
PaxDb	P18475.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0088938; FBpp0088012; FBgn0003733. FBtr0112838; FBpp0111751; FBgn0003733.
GeneID	35717.
KEGG	dme:Dmel_CG1389.
UCSC	CG1389-RA. d. melanogaster.
Organism-specific databases
CTD	21977.
FlyBase	FBgn0003733. tor.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00670000097694.
InParanoid	P18475.
KO	K12378.
OMA	RDVYHEN.
OrthoDB	EOG4PG4FT.
PhylomeDB	P18475.
Enzyme and pathway databases
BRENDA	2.7.10.1. 1994.
Gene expression databases
Bgee	P18475.
GermOnline	CG1389. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003961. Fibronectin_type3. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SMART	SM00060. FN3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	35717.
NextBio	794882.

Entry information

Entry name

TORSO_DROME

Accession

Primary (citable) accession number: P18475
Secondary accession number(s): A8DY54, A8DY56, Q9V4R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	August 30, 2005
Last modified:	May 1, 2013
This is version 132 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project