Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

H-2 class II histocompatibility antigen, I-A beta chain

Gene

H2-Eb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: BHF-UCL
  • response to interferon-gamma Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
H-2 class II histocompatibility antigen, I-A beta chain
Gene namesi
Name:H2-Eb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:95901. H2-Eb1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 225194ExtracellularSequence analysisAdd
BLAST
Transmembranei226 – 24823HelicalSequence analysisAdd
BLAST
Topological domaini249 – 26416CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, MHC II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Add
BLAST
Chaini32 – 264233H-2 class II histocompatibility antigen, I-A beta chainPRO_0000019003Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 106PROSITE-ProRule annotation1 Publication
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence analysis
Disulfide bondi144 ↔ 200PROSITE-ProRule annotation1 Publication

Post-translational modificationi

Ubiquitinated in immature dendritic cells leading to down-regulation of MHC class II.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiP18468.
PRIDEiP18468.

PTM databases

iPTMnetiP18468.
PhosphoSiteiP18468.

Expressioni

Gene expression databases

CleanExiMM_H2-EB1.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074143.

Structurei

Secondary structure

1
264
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 4512Combined sources
Helixi47 – 493Combined sources
Beta strandi50 – 5910Combined sources
Beta strandi62 – 687Combined sources
Turni69 – 713Combined sources
Beta strandi73 – 786Combined sources
Helixi79 – 813Combined sources
Helixi82 – 898Combined sources
Helixi92 – 10413Combined sources
Helixi106 – 1138Combined sources
Turni114 – 1163Combined sources
Beta strandi125 – 1317Combined sources
Beta strandi133 – 1353Combined sources
Beta strandi141 – 15212Combined sources
Beta strandi155 – 1606Combined sources
Beta strandi167 – 1715Combined sources
Beta strandi178 – 1803Combined sources
Beta strandi182 – 1898Combined sources
Beta strandi198 – 2036Combined sources
Beta strandi211 – 2155Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R5VX-ray2.50B/D32-215[»]
ProteinModelPortaliP18468.
SMRiP18468. Positions 31-215.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18468.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 21491Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni32 – 12190Beta-1Add
BLAST
Regioni122 – 21594Beta-2Add
BLAST
Regioni216 – 22510Connecting peptide

Sequence similaritiesi

Belongs to the MHC class II family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWG3. Eukaryota.
ENOG410YI0S. LUCA.
HOVERGENiHBG012730.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR000353. MHC_II_b_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00969. MHC_II_beta. 1 hit.
[Graphical view]
ProDomiPD000328. MHC_II_b_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00407. IGc1. 1 hit.
SM00921. MHC_II_beta. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVWLPRVPCV AAVILLLTVL SPPVALVRDS RPWFLEYCKS ECHFYNGTQR
60 70 80 90 100
VRFLKRYFYN LEENLRFDSD VGEFRAVTEL GRPDAENWNS QPEILDEKRA
110 120 130 140 150
AVDTYCRHNY EIFDNFLVPR RVEPTVTVYP TKTQPLEHHN LLVCSVSDFY
160 170 180 190 200
PGNIEVRWFR NGKEEKTGIV STGLVRNGDW TFQTLVMLET VPQSGEVYTC
210 220 230 240 250
QVEHPSLTDP VTVEWKAQST SAQNKMLSGV GGFVLGLLFL RAGLFIYFRN
260
QKGQSGLQPT GLLS
Length:264
Mass (Da):30,232
Last modified:November 1, 1990 - v1
Checksum:i04529F5E2E527D7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52641 mRNA. Translation: CAA36863.1.
PIRiI48224. S11650.
UniGeneiMm.22564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52641 mRNA. Translation: CAA36863.1.
PIRiI48224. S11650.
UniGeneiMm.22564.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R5VX-ray2.50B/D32-215[»]
ProteinModelPortaliP18468.
SMRiP18468. Positions 31-215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074143.

PTM databases

iPTMnetiP18468.
PhosphoSiteiP18468.

Proteomic databases

PaxDbiP18468.
PRIDEiP18468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:95901. H2-Eb1.

Phylogenomic databases

eggNOGiENOG410IWG3. Eukaryota.
ENOG410YI0S. LUCA.
HOVERGENiHBG012730.

Miscellaneous databases

EvolutionaryTraceiP18468.
SOURCEiSearch...

Gene expression databases

CleanExiMM_H2-EB1.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR000353. MHC_II_b_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00969. MHC_II_beta. 1 hit.
[Graphical view]
ProDomiPD000328. MHC_II_b_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00407. IGc1. 1 hit.
SM00921. MHC_II_beta. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nonobese diabetic and nonobese nondiabetic mice have unique MHC class II haplotypes."
    Acha-Orbea H., Scarpellino L.
    Immunogenetics 34:57-59(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NOD.
    Tissue: Spleen.
  2. "Dendritic cells regulate exposure of MHC class II at their plasma membrane by oligoubiquitination."
    van Niel G., Wubbolts R., Ten Broeke T., Buschow S.I., Ossendorp F.A., Melief C.J., Raposo G., van Balkom B.W., Stoorvogel W.
    Immunity 25:885-894(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.
  3. "Surface expression of MHC class II in dendritic cells is controlled by regulated ubiquitination."
    Shin J.S., Ebersold M., Pypaert M., Delamarre L., Hartley A., Mellman I.
    Nature 444:115-118(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.
  4. "Evidence that structural rearrangements and/or flexibility during TCR binding can contribute to T cell activation."
    Krogsgaard M., Prado N., Adams E.J., He X.L., Chow D.C., Wilson D.B., Garcia K.C., Davis M.M.
    Mol. Cell 12:1367-1378(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 32-215, DISULFIDE BONDS.

Entry informationi

Entry nameiHB2I_MOUSE
AccessioniPrimary (citable) accession number: P18468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: January 20, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.