P18465 (1B57_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class I histocompatibility antigen, B-57 alpha chain

Alternative name(s):
Bw-57
MHC class I antigen B*57

Gene names

Name:	HLA-B
Synonyms:	HLAB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.
Polymorphism	The following alleles of B-57 are known: B*57:01, B*57:02 (Bw57.2), B*57:03 (B-57SAU) and B*57:05. The sequence shown is that of B*57:01.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Immunity
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Traceable author statement. Source: Reactome antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent Traceable author statement. Source: Reactome interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome regulation of immune response Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ER to Golgi transport vesicle membrane Traceable author statement. Source: Reactome Golgi membrane Traceable author statement. Source: Reactome MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW early endosome membrane Traceable author statement. Source: Reactome integral to lumenal side of endoplasmic reticulum membrane Traceable author statement. Source: Reactome phagocytic vesicle membrane Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Chain

25 – 362

338

HLA class I histocompatibility antigen, B-57 alpha chain

PRO_0000018860

Regions

Topological domain

25 – 308

284

Extracellular Potential

Transmembrane

309 – 332

24

Helical; Potential

Topological domain

333 – 362

30

Cytoplasmic Potential

Domain

209 – 295

87

Ig-like C1-type

Region

25 – 114

90

Alpha-1

Region

115 – 206

92

Alpha-2

Region

207 – 298

92

Alpha-3

Region

299 – 308

10

Connecting peptide

Amino acid modifications

Glycosylation

110

1

N-linked (GlcNAc...) By similarity

Disulfide bond

125 ↔ 188

Ref.8

Disulfide bond

227 ↔ 283

Ref.8

Natural variations

Natural variant

121

1

V → R in allele B*57:05; requires 2 nucleotide substitutions.

VAR_016669

Natural variant

127

1

V → L in allele B*57:05.

VAR_016670

Natural variant

137

1

H → Y in allele B*57:05.

VAR_016671

Natural variant

138

1

D → N in allele B*57:02, allele B*57:03 and allele B*57:05.

VAR_016495

Natural variant

140

1

S → Y in allele B*57:02, allele B*57:03 and allele B*57:05.

VAR_016496

Natural variant

180

1

L → R in allele B*57:02 and allele B*57:05.

VAR_016497

Natural variant

306

1

V → I.
Corresponds to variant rs1131500 [ dbSNP | Ensembl ].

VAR_056416

Natural variant

329

1

A → T.
Corresponds to variant rs1051488 [ dbSNP | Ensembl ].

VAR_056417

Natural variant

349

1

C → S.
Corresponds to variant rs2308655 [ dbSNP | Ensembl ].

VAR_061431

Natural variant

349

1

C → Y.
Corresponds to variant rs2308655 [ dbSNP | Ensembl ].

VAR_061432

Secondary structure

1

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362

Helix Strand Turn

Details...

Beta strand

27 – 36

10

Beta strand

41 – 43

3

Beta strand

45 – 52

8

Beta strand

55 – 61

7

Beta strand

64 – 66

3

Helix

74 – 76

3

Helix

81 – 108

28

Beta strand

118 – 127

10

Beta strand

133 – 142

10

Beta strand

145 – 150

6

Beta strand

157 – 161

5

Helix

162 – 173

12

Helix

176 – 185

10

Helix

187 – 198

12

Helix

200 – 203

4

Beta strand

210 – 219

10

Beta strand

222 – 235

14

Beta strand

238 – 243

6

Helix

249 – 251

3

Beta strand

261 – 263

3

Beta strand

265 – 274

10

Helix

278 – 280

3

Beta strand

281 – 286

6

Beta strand

290 – 292

3

Beta strand

294 – 296

3

Sequences

	Sequence		Length	Mass (Da)
	P18465 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: C1EFCF089096AFF9	FASTA	362	40,224
	10 20 30 40 50 60 MRVTAPRTVL LLLWGAVALT ETWAGSHSMR YFYTAMSRPG RGEPRFIAVG YVDDTQFVRF 70 80 90 100 110 120 DSDAASPRMA PRAPWIEQEG PEYWDGETRN MKASAQTYRE NLRIALRYYN QSEAGSHIIQ 130 140 150 160 170 180 VMYGCDVGPD GRLLRGHDQS AYDGKDYIAL NEDLSSWTAA DTAAQITQRK WEAARVAEQL 190 200 210 220 230 240 RAYLEGLCVE WLRRYLENGK ETLQRADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT 250 260 270 280 290 300 WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP 310 320 330 340 350 360 SSQSTVPIVG IVAGLAVLAV VVIGAVVAAV MCRRKSSGGK GGSYSQAACS DSAQGSDVSL TA

References

[1]	"Rapid cloning of HLA-A,B cDNA by using the polymerase chain reaction: frequency and nature of errors produced in amplification." Ennis P.D., Zemmour J., Salter R.D., Parham P. Proc. Natl. Acad. Sci. U.S.A. 87:2833-2837(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*57:01).
[2]	"Nucleotide sequence of an HLA-Bw57 gene." Isamat M., Girdlestone J., Milstein C. Nucleic Acids Res. 18:6702-6702(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE B*57:01).
[3]	"Confirmation of B*5701." Cox S.T. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Distinctive HLA-A,B antigens of black populations formed by interallelic conversion." Madrigal J.A., Belich M.P., Hildebrand W.H., Benjamin R.J., Little A.-M., Zemmour J., Ennis P.D., Ward F.E., Petzl-Erler M.L., Martell R.W., du Toit E.D., Parham P. J. Immunol. 149:3411-3415(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*57:02).
[5]	"Molecular diversity of HLA-B." Petersdorf E. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE B*57:03). Tissue: Blood.
[6]	"The novel HLA-Cw*1802 allele is associated with B*5703 in the Bubi population from Equatorial Guinea." Vilches C., Bunce M., de Pablo R., Moreno M.E., Puente S., Sanz L., Kreisler M. Tissue Antigens 49:644-648(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*57:03).
[7]	"Twenty-nine new HLA-B alleles associated with antigens in the 5C CREG." Steiner N.K., Kosman C., Jones P.F., Gans C.P., Rodriguez-Marino S.G., Rizzuto G., Baldassarre L.A., Edson S., Koester R., Sese D., Mitton W., Ng J., Hartzman R.J., Hurley C.K. Tissue Antigens 57:481-485(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE B*57:05).
[8]	"Structures of three HIV-1 HLA-B*5703-peptide complexes and identification of related HLAs potentially associated with long-term nonprogression." Stewart-Jones G.B., Gillespie G., Overton I.M., Kaul R., Roche P., McMichael A.J., Rowland-Jones S., Jones E.Y. J. Immunol. 175:2459-2468(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 25-300 (ALLELE B*57:03) IN COMPLEX WITH HIV-1 PEPTIDES, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M32318 mRNA. Translation: AAA36231.1.
X55711 Genomic DNA. Translation: CAA39244.1.
AJ458991 Genomic DNA. Translation: CAD30339.1.
X61707 mRNA. Translation: CAA43876.1.
U18790 mRNA. Translation: AAB60361.1.
Y09157 mRNA. Translation: CAA70355.1.
AH006370 Genomic DNA. Translation: AAC32568.1.

IPI

IPI00472434.

PIR

D35997.

UniGene

Hs.654404.
Hs.77961.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BVO	X-ray	1.65	A	25-300	[»]
2BVP	X-ray	1.35	A	25-300	[»]
2BVQ	X-ray	2.00	A	25-300	[»]
2HJL	X-ray	1.50	A	25-298	[»]
2RFX	X-ray	2.50	A	25-299	[»]
2YPK	X-ray	1.95	A	25-298	[»]
2YPL	X-ray	2.40	A	25-298	[»]
3UPR	X-ray	2.00	A/C	25-300	[»]
3VH8	X-ray	1.80	A/D	25-299	[»]
3VRI	X-ray	1.60	A	25-300	[»]
3VRJ	X-ray	1.90	A	25-300	[»]

ProteinModelPortal

P18465.

ModBase

Search...

Protein-protein interaction databases

IntAct

P18465. 1 interaction.

Polymorphism databases

DMDM

122174.

Proteomic databases

PRIDE

P18465.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000435618; ENSP00000405178; ENSG00000224608.

Organism-specific databases

GeneCards

GC06M031321.

HGNC

HGNC:4932. HLA-B.

MIM

142830. gene.

neXtProt

NX_P18465.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG016709.

InParanoid

P18465.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P18465.

CleanEx

HS_HLA-B.

Genevestigator

P18465.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
3.30.500.10. 1 hit.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]

Pfam

PF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]

PRINTS

PR01638. MHCCLASSI.

SMART

SM00407. IGc1. 1 hit.
[Graphical view]

SUPFAM

SSF54452. MHC_I/II-like_Ag-recog. 1 hit.

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

HLA-B. human.

EvolutionaryTrace

P18465.

SOURCE

Search...

Entry information

Entry name

1B57_HUMAN

Accession

Primary (citable) accession number: P18465
Secondary accession number(s): O62917 , P30497, P79496, Q29679, Q5QT24

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.