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Protein

T-cell surface glycoprotein CD3 delta chain

Gene

CD3D

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 delta chain
Alternative name(s):
T-cell receptor T3 delta chain
CD_antigen: CD3d
Gene namesi
Name:CD3D
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 100ExtracellularSequence analysisAdd BLAST79
Transmembranei101 – 121HelicalSequence analysisAdd BLAST21
Topological domaini122 – 167CytoplasmicSequence analysisAdd BLAST46

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001649222 – 167T-cell surface glycoprotein CD3 delta chainAdd BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 73By similarity
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei145PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei156PhosphotyrosinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP18438.

Expressioni

Tissue specificityi

CD3D is mostly present on T-lymphocytes with its TCR-CD3 partners. Present also in fetal NK-cells.By similarity

Interactioni

Subunit structurei

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with coreceptors CD4 and CD8.By similarity

Structurei

Secondary structure

1167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 29Combined sources8
Beta strandi32 – 36Combined sources5
Beta strandi38 – 40Combined sources3
Beta strandi42 – 46Combined sources5
Beta strandi51 – 58Combined sources8
Beta strandi68 – 72Combined sources5
Beta strandi75 – 77Combined sources3
Beta strandi81 – 86Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XMWNMR-A22-88[»]
ProteinModelPortaliP18438.
SMRiP18438.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18438.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 162ITAMPROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG005278.
KOiK06450.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015484. CD3_esu/gsu/dsu.
IPR015485. CD3D.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR032052. Ig_4.
IPR003110. Phos_immunorcpt_sig_ITAM.
PANTHERiPTHR10570. PTHR10570. 1 hit.
PTHR10570:SF5. PTHR10570:SF5. 1 hit.
PfamiView protein in Pfam
PF16680. Ig_4. 1 hit.
PF02189. ITAM. 1 hit.
SMARTiView protein in SMART
SM00077. ITAM. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS51055. ITAM_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18438-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHSRCLSCL ILAALLSQVN PRALEVLEAE DKVILKCNSS ITLLQGTAGQ
60 70 80 90 100
EVSDNKTLNL GKRIEDPRGM YQCGENAKSF TLQVYYRMCQ NCVELDSATL
110 120 130 140 150
AGLIITDIIA TVLLALGVYC FAGHETGRFS RAADTQVLMG NDQLYQPLRE
160
RNDAQYSRLG DKWARNK
Length:167
Mass (Da):18,562
Last modified:November 1, 1990 - v1
Checksum:i35D55493AE369714
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52993 mRNA. Translation: CAA37182.1.
PIRiB43547. RWSHD3.
RefSeqiNP_001009382.1. NM_001009382.1.
UniGeneiOar.427.

Genome annotation databases

GeneIDi443397.
KEGGioas:443397.

Similar proteinsi

Entry informationi

Entry nameiCD3D_SHEEP
AccessioniPrimary (citable) accession number: P18438
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: February 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome