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Protein

Protein kinase shaggy

Gene

sgg

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for several developmental events such as syncytial blastoderm formation and embryonic segmentation. Is involved in transcriptional regulation. Required for arm phosphorylation. Wg signaling operates by inactivating the sgg repression of en autoactivation. Negatively controls the neuromuscular junction (NMJ) growth in presynaptic motoneurons. Plays a role in the regulation of microtubule dynamics and actin cytoskeleton during embryogenesis. Required for phosphorylation of sra in activated eggs. Essential for completion of meiosis, possibly by triggering calcineurin activation via sra phosphorylation. Phosphorylates microtubule-associated protein futsch in axons.8 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei83ATPPROSITE-ProRule annotation1
Active sitei179Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi60 – 68ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • chaeta development Source: FlyBase
  • chaeta morphogenesis Source: FlyBase
  • chitin-based larval cuticle pattern formation Source: FlyBase
  • circadian rhythm Source: FlyBase
  • entrainment of circadian clock Source: FlyBase
  • epithelial cell morphogenesis Source: FlyBase
  • establishment of epithelial cell planar polarity Source: FlyBase
  • female meiotic nuclear division Source: FlyBase
  • habituation Source: FlyBase
  • heart development Source: FlyBase
  • imaginal disc-derived wing margin morphogenesis Source: FlyBase
  • locomotor rhythm Source: FlyBase
  • negative regulation of canonical Wnt signaling pathway Source: FlyBase
  • negative regulation of insulin receptor signaling pathway Source: FlyBase
  • negative regulation of JUN kinase activity Source: FlyBase
  • negative regulation of smoothened signaling pathway Source: FlyBase
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • olfactory learning Source: FlyBase
  • oogenesis Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • positive regulation of mitotic metaphase/anaphase transition Source: FlyBase
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
  • positive regulation of protein catabolic process Source: FlyBase
  • positive regulation of proteolysis involved in cellular protein catabolic process Source: FlyBase
  • positive regulation of ubiquitin-dependent protein catabolic process Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • pseudocleavage involved in syncytial blastoderm formation Source: UniProtKB
  • regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
  • regulation of circadian rhythm Source: FlyBase
  • regulation of hemocyte differentiation Source: FlyBase
  • regulation of microtubule cytoskeleton organization Source: UniProtKB
  • regulation of protein import into nucleus Source: FlyBase
  • regulation of proteolysis Source: FlyBase
  • response to anesthetic Source: FlyBase
  • rhythmic behavior Source: FlyBase
  • segment polarity determination Source: FlyBase
  • somatic stem cell population maintenance Source: FlyBase
  • spindle organization Source: FlyBase
  • synaptic growth at neuromuscular junction Source: FlyBase
  • wing and notum subfield formation Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, Kinase, Segmentation polarity protein, Serine/threonine-protein kinase, Transferase
Biological processMeiosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.26 1994
ReactomeiR-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-196299 Beta-catenin phosphorylation cascade
R-DME-209155 Phosphorylation of AXN and APC
R-DME-209159 Assembly of the CI containing complexes
R-DME-209190 Phosphorylation of CI
R-DME-209214 Phosphorylation of SMO
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209387 Phosphorylation of ARR
R-DME-209396 Phosphorylation of ARM
R-DME-209413 Assembly of the 'destruction complex'
R-DME-209440 Recruitment of the 'destruction complex' to the receptor complex, the degradation of AXN and release of ARM
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-3371453 Regulation of HSF1-mediated heat shock response
R-DME-432553 Phosphorylation of PER and TIM
R-DME-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
SignaLinkiP18431

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase shaggy (EC:2.7.11.1)
Alternative name(s):
Protein zeste-white 3
Gene namesi
Name:sgg
Synonyms:gsk3, zw3
ORF Names:CG2621
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0003371 sgg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus, Synapse

Pathology & Biotechi

Disruption phenotypei

Mutants display an overdeveloped neuromuscular junction (NMJ), with the number of boutons greatly increased.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81A → T: Increases the amount of synaptic boutons and microtubule loops when expressed presynaptically. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866411 – 514Protein kinase shaggyAdd BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei213Phosphoserine1 Publication1
Modified residuei214Phosphotyrosine2 Publications1
Modified residuei217Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP18431
PRIDEiP18431

PTM databases

iPTMnetiP18431

Expressioni

Tissue specificityi

Expressed in ovaries and activated eggs (at protein level). Expression is over all the embryo at all stages, no local accumulation is observed.3 Publications

Developmental stagei

Isoform SGG46 is expressed at low levels in 12-24 hours embryos. Isoform Zygotic and isoform SGG39 are expressed in 12-24 hours embryos and present throughout the larval, pupal and adult stages (at protein level). Isoform Zygotic is expressed maternally and zygotically but reduced throughout later embryonic development. Expression persists throughout larval stages.2 Publications

Gene expression databases

BgeeiFBgn0003371
ExpressionAtlasiP18431 baseline and differential
GenevisibleiP18431 DM

Interactioni

Subunit structurei

Interacts with cos.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
cryO770592EBI-242141,EBI-94117

Protein-protein interaction databases

BioGridi57779, 178 interactors
DIPiDIP-39170N
IntActiP18431, 698 interactors
STRINGi7227.FBpp0304140

Structurei

3D structure databases

ProteinModelPortaliP18431
SMRiP18431
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 338Protein kinasePROSITE-ProRule annotationAdd BLAST285

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi386 – 509Ala/Gly-richAdd BLAST124

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0658 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00520000055635
InParanoidiP18431
KOiK03083
OMAiHRIIPEQ
OrthoDBiEOG091G099S

Family and domain databases

InterProiView protein in InterPro
IPR033573 GSK3B
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24057:SF8 PTHR24057:SF8, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform Zygotic (identifier: P18431-2) [UniParc]FASTAAdd to basket
Also known as: B, C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGRPRTSSF AEGNKQSPSL VLGGVKTCSR DGSKITTVVA TPGQGTDRVQ
60 70 80 90 100
EVSYTDTKVI GNGSFGVVFQ AKLCDTGELV AIKKVLQDRR FKNRELQIMR
110 120 130 140 150
KLEHCNIVKL LYFFYSSGEK RDEVFLNLVL EYIPETVYKV ARQYAKTKQT
160 170 180 190 200
IPINFIRLYM YQLFRSLAYI HSLGICHRDI KPQNLLLDPE TAVLKLCDFG
210 220 230 240 250
SAKQLLHGEP NVSYICSRYY RAPELIFGAI NYTTKIDVWS AGCVLAELLL
260 270 280 290 300
GQPIFPGDSG VDQLVEVIKV LGTPTREQIR EMNPNYTEFK FPQIKSHPWQ
310 320 330 340 350
KVFRIRTPTE AINLVSLLLE YTPSARITPL KACAHPFFDE LRMEGNHTLP
360 370 380 390 400
NGRDMPPLFN FTEHELSIQP SLVPQLLPKH LQNASGPGGN RPSAGGAASI
410 420 430 440 450
AASGSTSVSS TGSGASVEGS AQPQSQGTAA AAGSGSGGAT AGTGGASAGG
460 470 480 490 500
PGSGNNSSSG GASGAPSAVA AGGANAAVAG GAGGGGGAGA ATAAATATGA
510
IGATNAGGAN VTDS
Note: Major isoform.
Length:514
Mass (Da):53,872
Last modified:September 5, 2012 - v3
Checksum:i193EE2A1294BE494
GO
Isoform SGG46 (identifier: P18431-1) [UniParc]FASTAAdd to basket
Also known as: Maternal, D

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MSGRPRTSSFAEGNKQSPSLVLGGVKTCS → MATTTTTQRA...VVQKFKNILG

Show »
Length:1,067
Mass (Da):114,440
Checksum:iB4E98AFBF4807979
GO
Isoform SGG39 (identifier: P18431-3) [UniParc]FASTAAdd to basket
Also known as: A, J

The sequence of this isoform differs from the canonical sequence as follows:
     513-514: DS → GSQSNSALNSSGSGGSGNGEAAGSGSGSGSGSGGGNGGDNDAGDSGAIASGGGAAETEAAASG

Show »
Length:575
Mass (Da):58,772
Checksum:i477B3E036464E4D5
GO
Isoform G (identifier: P18431-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MSGRPRTSSFAEGNKQSPSLVLGGVKTCS → MLINRGSLLEG

Note: No experimental confirmation available.
Show »
Length:496
Mass (Da):52,092
Checksum:i8059B6236E7B268D
GO

Sequence cautioni

The sequence CAA37952 differs from that shown. Reason: Frameshift at position 473.Curated
The sequence CAA50216 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244V → I in CAA37419 (PubMed:8467811).Curated1
Sequence conflicti397A → R in CAA37952 (PubMed:2113617).Curated1
Sequence conflicti406T → A in CAA37419 (PubMed:8467811).Curated1
Sequence conflicti406T → A in AAM50318 (PubMed:2113617).Curated1
Sequence conflicti446A → R in CAA37952 (PubMed:2113617).Curated1
Sequence conflicti462A → R in CAA37952 (PubMed:2113617).Curated1
Sequence conflicti473Missing in CAA37952 (PubMed:2113617).Curated1
Sequence conflicti513 – 514DS → GE in CAA19676 (PubMed:10731137).Curated2
Sequence conflicti513 – 514DS → GE in CAB65860 (PubMed:10731137).Curated2
Isoform SGG46 (identifier: P18431-1)
Sequence conflicti9R → A in AAF45801 (PubMed:10731132).Curated1
Sequence conflicti9R → A in CAA19676 (PubMed:10731137).Curated1
Sequence conflicti9R → A in CAB65860 (PubMed:10731137).Curated1
Sequence conflicti256 – 259EEEE → E in CAA37952 (PubMed:2113617).Curated4
Sequence conflicti258 – 259EE → E in CAA19676 (PubMed:10731137).Curated2
Sequence conflicti258 – 259EE → E in CAB65860 (PubMed:10731137).Curated2
Sequence conflicti539 – 540AD → RI in CAA37952 (PubMed:2113617).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0441041 – 29MSGRP…VKTCS → MLINRGSLLEG in isoform G. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_0441051 – 29MSGRP…VKTCS → MATTTTTQRAGAAPALNLLP ASNNNINNTLINNNNNNNNT SNSNNNNNNVISQPIKIPLT ERFSSQTSTGSADSGVIVSS ASQQQLQLPPPRSSSGSLSL PQAPPGGKWRQKQQRQQLLL SQDSGIENGVTTRPSKAKDN QGAGKASHNATSSKESGAQS NSSSESLGSNCSEAQEQQRV RASSALELSSVDTPVIVGGV VSGGNSILRSRIKYKSTNST GTQGFDVEDRIDEVDICDDD DVDCDDRGSEIEEEEEEEED DGVNVDDDVEEADNQSDNQS GIIINLKSQTEQEEEVDEVD AKPKNRLLPPDQAELTVAAA MARRRDAKSLATDGHIYFPL LKISEDPHIDSKLINRKDGL QDTMYYLDEFGSPKLREKFA RKQKQLLAKQQKQLMKRERR SEEQRKKRNTTVASNLAASG AVVDDTKDDYKQQPHCDTSS RSKNNSVPNPPSSHLHQNHN HLVVDVQEDVDDVNVVATSD VDSGVVKMRRHSHDNHYDRI PRSNAATITTRPQIDQQSSH HQNTEDVEQGAEPQIDGEAD LDADADADSDGSGENVKTAK LARTQSCVSWTKVVQKFKNI LG in isoform SGG46. 2 PublicationsAdd BLAST29
Alternative sequenceiVSP_044106513 – 514DS → GSQSNSALNSSGSGGSGNGE AAGSGSGSGSGSGGGNGGDN DAGDSGAIASGGGAAETEAA ASG in isoform SGG39. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70862 mRNA Translation: CAA50212.1
X70863 mRNA Translation: CAA50213.1
X70864 mRNA Translation: CAA50214.1
X70865 mRNA Translation: CAA50215.1
X70866 mRNA Translation: CAA50216.1 Sequence problems.
X53332 mRNA Translation: CAA37419.1
AE014298 Genomic DNA Translation: AAF45801.2
AE014298 Genomic DNA Translation: AAN09082.1
AE014298 Genomic DNA Translation: AAN09083.1
AE014298 Genomic DNA Translation: AAS65255.1
AL024485, AL034544 Genomic DNA Translation: CAA19676.1
AL121804, AL024485 Genomic DNA Translation: CAB65860.1
AL121804, AL024485 Genomic DNA Translation: CAB72296.1
AY122193 mRNA Translation: AAM52705.1
AY119664 mRNA Translation: AAM50318.1
X54005 mRNA Translation: CAA37951.1
X54006 mRNA Translation: CAA37952.1 Frameshift.
PIRiS35325
S35327
S35328 S35423
RefSeqiNP_476714.1, NM_057366.5 [P18431-3]
NP_476715.1, NM_057367.5 [P18431-2]
NP_476716.2, NM_057368.5
NP_599105.1, NM_134278.3 [P18431-2]
NP_726822.1, NM_166947.3 [P18431-2]
NP_726823.1, NM_166948.4 [P18431-2]
NP_996335.1, NM_206612.3 [P18431-4]
NP_996336.1, NM_206613.3 [P18431-3]
NP_996337.1, NM_206614.3 [P18431-2]
NP_996338.1, NM_206615.2 [P18431-2]
UniGeneiDm.7795

Genome annotation databases

EnsemblMetazoaiFBtr0070466; FBpp0070449; FBgn0003371 [P18431-2]
FBtr0070467; FBpp0070450; FBgn0003371 [P18431-3]
FBtr0070468; FBpp0070451; FBgn0003371 [P18431-2]
FBtr0070469; FBpp0070452; FBgn0003371 [P18431-2]
FBtr0070470; FBpp0070453; FBgn0003371 [P18431-2]
FBtr0070472; FBpp0089162; FBgn0003371 [P18431-2]
FBtr0070473; FBpp0089158; FBgn0003371 [P18431-2]
FBtr0070474; FBpp0089159; FBgn0003371 [P18431-3]
FBtr0070475; FBpp0089160; FBgn0003371 [P18431-4]
GeneIDi31248
KEGGidme:Dmel_CG2621

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSGG_DROME
AccessioniPrimary (citable) accession number: P18431
Secondary accession number(s): O76881
, P23646, Q27603, Q27604, Q27605, Q8MRF7, Q9NF42, Q9U094, Q9W4X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: September 5, 2012
Last modified: June 20, 2018
This is version 209 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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