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P18421

- PSB1_RAT

UniProt

P18421 - PSB1_RAT

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Protein

Proteasome subunit beta type-1

Gene

Psmb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.986.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-1 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C5
Multicatalytic endopeptidase complex subunit C5
Proteasome component C5
Proteasome gamma chain
Gene namesi
Name:Psmb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621092. Psmb1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome core complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 27271 PublicationPRO_0000259625Add
BLAST
Chaini28 – 240213Proteasome subunit beta type-1PRO_0000148032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi57 – 571O-linked (GlcNAc)By similarity
Modified residuei149 – 1491PhosphotyrosineBy similarity
Modified residuei203 – 2031N6-acetyllysineBy similarity
Glycosylationi208 – 2081O-linked (GlcNAc)By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP18421.
PRIDEiP18421.

PTM databases

PhosphoSiteiP18421.

Expressioni

Tissue specificityi

Ubiquitous.

Inductioni

Up-regulated in prefrontal cortex (PFC) after nicotine exposure. Down-regulated by theophylline (THP) and 1,3-dinitrobenzene (DNB), two reprotoxic agents thought to induce infertility.2 Publications

Gene expression databases

GenevestigatoriP18421.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with SERPINB2 By similarity.By similarity

Protein-protein interaction databases

IntActiP18421. 2 interactions.
STRINGi10116.ENSRNOP00000002037.

Structurei

3D structure databases

ProteinModelPortaliP18421.
SMRiP18421. Positions 28-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091081.
HOVERGENiHBG000961.
InParanoidiP18421.
KOiK02732.
PhylomeDBiP18421.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18421-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSTAAYRDP DRELVMGPQG SAGPVQMRFS PYAFNGGTVL AIAGEDFSIV
60 70 80 90 100
ASDTRLSEGF SIHTRDSPKC YKLTDKTVIG CSGFHGDCLT LTKIIEARLK
110 120 130 140 150
MYKHSNNKAM TTGAIAAMLS TILYSRRFFP YYVYNIIEGL DEEGKGAVYS
160 170 180 190 200
FDPVGSYQRD SFKAGGSASA MLQPLLDNQV GFKNMQNVEH VPLTLDRAMR
210 220 230 240
LVKDVFISAA ERDVYTGDAL RICIVTKEGI REETVPLRKD
Length:240
Mass (Da):26,479
Last modified:May 1, 1992 - v3
Checksum:iB79EB682CDB95A01
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52783 mRNA. Translation: CAA36987.1.
PIRiS09696.
RefSeqiNP_446042.1. NM_053590.1.
UniGeneiRn.6016.

Genome annotation databases

GeneIDi94198.
KEGGirno:94198.
UCSCiRGD:621092. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52783 mRNA. Translation: CAA36987.1 .
PIRi S09696.
RefSeqi NP_446042.1. NM_053590.1.
UniGenei Rn.6016.

3D structure databases

ProteinModelPortali P18421.
SMRi P18421. Positions 28-240.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P18421. 2 interactions.
STRINGi 10116.ENSRNOP00000002037.

Protein family/group databases

MEROPSi T01.986.

PTM databases

PhosphoSitei P18421.

Proteomic databases

PaxDbi P18421.
PRIDEi P18421.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 94198.
KEGGi rno:94198.
UCSCi RGD:621092. rat.

Organism-specific databases

CTDi 5689.
RGDi 621092. Psmb1.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000091081.
HOVERGENi HBG000961.
InParanoidi P18421.
KOi K02732.
PhylomeDBi P18421.

Miscellaneous databases

NextBioi 617862.
PROi P18421.

Gene expression databases

Genevestigatori P18421.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and sequencing of component C5 of proteasomes from rat hepatoma cells."
    Tamura T., Tanaka K., Kumatori A., Yamada F., Tsurumi C., Fujiwara T., Ichihara A., Tokunaga F., Aruga R., Iwanaga S.
    FEBS Lett. 264:91-94(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The NH2-terminal residues of rat liver proteasome (multicatalytic proteinase complex) subunits, C2, C3 and C8, are N alpha-acetylated."
    Tokunaga F., Aruga R., Iwanaga S., Tanaka K., Ichihara A., Takao T., Shimonishi Y.
    FEBS Lett. 263:373-375(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-47.
    Tissue: Liver.
  3. "Nicotine coregulates multiple pathways involved in protein modification/degradation in rat brain."
    Kane J.K., Konu O., Ma J.Z., Li M.D.
    Brain Res. Mol. Brain Res. 132:181-191(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NICOTINE.
  4. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
    Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
    Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY THP AND DNB.

Entry informationi

Entry nameiPSB1_RAT
AccessioniPrimary (citable) accession number: P18421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3