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Protein

Proteasome subunit beta type-1

Gene

Psmb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.986.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-1 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C5
Multicatalytic endopeptidase complex subunit C5
Proteasome component C5
Proteasome gamma chain
Gene namesi
Name:Psmb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621092. Psmb1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. proteasome core complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 27271 PublicationPRO_0000259625Add
BLAST
Chaini28 – 240213Proteasome subunit beta type-1PRO_0000148032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi57 – 571O-linked (GlcNAc)By similarity
Modified residuei149 – 1491PhosphotyrosineBy similarity
Modified residuei203 – 2031N6-acetyllysineBy similarity
Glycosylationi208 – 2081O-linked (GlcNAc)By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP18421.
PRIDEiP18421.

PTM databases

PhosphoSiteiP18421.

Expressioni

Tissue specificityi

Ubiquitous.

Inductioni

Up-regulated in prefrontal cortex (PFC) after nicotine exposure. Down-regulated by theophylline (THP) and 1,3-dinitrobenzene (DNB), two reprotoxic agents thought to induce infertility.2 Publications

Gene expression databases

GenevestigatoriP18421.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with SERPINB2 (By similarity).By similarity

Protein-protein interaction databases

IntActiP18421. 2 interactions.
STRINGi10116.ENSRNOP00000002037.

Structurei

3D structure databases

ProteinModelPortaliP18421.
SMRiP18421. Positions 28-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091081.
HOVERGENiHBG000961.
InParanoidiP18421.
KOiK02732.
PhylomeDBiP18421.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18421-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSTAAYRDP DRELVMGPQG SAGPVQMRFS PYAFNGGTVL AIAGEDFSIV
60 70 80 90 100
ASDTRLSEGF SIHTRDSPKC YKLTDKTVIG CSGFHGDCLT LTKIIEARLK
110 120 130 140 150
MYKHSNNKAM TTGAIAAMLS TILYSRRFFP YYVYNIIEGL DEEGKGAVYS
160 170 180 190 200
FDPVGSYQRD SFKAGGSASA MLQPLLDNQV GFKNMQNVEH VPLTLDRAMR
210 220 230 240
LVKDVFISAA ERDVYTGDAL RICIVTKEGI REETVPLRKD
Length:240
Mass (Da):26,479
Last modified:May 1, 1992 - v3
Checksum:iB79EB682CDB95A01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52783 mRNA. Translation: CAA36987.1.
PIRiS09696.
RefSeqiNP_446042.1. NM_053590.1.
UniGeneiRn.6016.

Genome annotation databases

GeneIDi94198.
KEGGirno:94198.
UCSCiRGD:621092. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52783 mRNA. Translation: CAA36987.1.
PIRiS09696.
RefSeqiNP_446042.1. NM_053590.1.
UniGeneiRn.6016.

3D structure databases

ProteinModelPortaliP18421.
SMRiP18421. Positions 28-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP18421. 2 interactions.
STRINGi10116.ENSRNOP00000002037.

Protein family/group databases

MEROPSiT01.986.

PTM databases

PhosphoSiteiP18421.

Proteomic databases

PaxDbiP18421.
PRIDEiP18421.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi94198.
KEGGirno:94198.
UCSCiRGD:621092. rat.

Organism-specific databases

CTDi5689.
RGDi621092. Psmb1.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091081.
HOVERGENiHBG000961.
InParanoidiP18421.
KOiK02732.
PhylomeDBiP18421.

Miscellaneous databases

NextBioi617862.
PROiP18421.

Gene expression databases

GenevestigatoriP18421.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and sequencing of component C5 of proteasomes from rat hepatoma cells."
    Tamura T., Tanaka K., Kumatori A., Yamada F., Tsurumi C., Fujiwara T., Ichihara A., Tokunaga F., Aruga R., Iwanaga S.
    FEBS Lett. 264:91-94(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The NH2-terminal residues of rat liver proteasome (multicatalytic proteinase complex) subunits, C2, C3 and C8, are N alpha-acetylated."
    Tokunaga F., Aruga R., Iwanaga S., Tanaka K., Ichihara A., Takao T., Shimonishi Y.
    FEBS Lett. 263:373-375(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-47.
    Tissue: Liver.
  3. "Nicotine coregulates multiple pathways involved in protein modification/degradation in rat brain."
    Kane J.K., Konu O., Ma J.Z., Li M.D.
    Brain Res. Mol. Brain Res. 132:181-191(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NICOTINE.
  4. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
    Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
    Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY THP AND DNB.

Entry informationi

Entry nameiPSB1_RAT
AccessioniPrimary (citable) accession number: P18421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 1, 1992
Last modified: March 4, 2015
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.