Reviewed, UniProtKB/Swiss-Prot P18420 (PSA1_RAT)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-1
    EC=3.4.25.1
Alternative name(s):
    Proteasome component C2
    Macropain subunit C2
    Multicatalytic endopeptidase complex subunit C2
    Proteasome nu chain

Gene names

Name:

Psma1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	Ubiquitous.
Post-translational modification	Proteolytically cleaved from a C-terminal extension in the course of the conversion of the proteasome from its latent form into its active form.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Proteasome subunit alpha type-1		PRO_0000124064
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.3
	Modified residue	14	1	Phosphoserine By similarity
	Cross-link	115		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	208		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18420-1 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: 68660696B43A4051	FASTA	263	29,518
	10 20 30 40 50 60 MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK RAQSELAAHQ 70 80 90 100 110 120 KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFD RPLPVSRLVS LIGSKTQIPT 130 140 150 160 170 180 QRYGRRPYGV GLLIAGYDDM GPHVFQTCPS ANYFDCRAMS IGARSQSART YLERHMSEFM 190 200 210 220 230 240 QCNLDELVKH GLRALRETLP AEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLDGLEERP 250 260 QRKAQPSQAA DEPAEKADEP MEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of cDNA for proteasomes (multicatalytic proteinase complexes) from rat liver: primary structure of the largest component (C2)." Fujiwara T., Tanaka K., Kumatori A., Shin S., Yoshimura T., Ichihara A., Tokunaga F., Aruga R., Iwanaga S., Kakizuka A., Nakanishi S. Biochemistry 28:7332-7340(1989) [PubMed: 2819072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[3]	"The NH2-terminal residues of rat liver proteasome (multicatalytic proteinase complex) subunits, C2, C3 and C8, are N alpha-acetylated." Tokunaga F., Aruga R., Iwanaga S., Tanaka K., Ichihara A., Takao T., Shimonishi Y. FEBS Lett. 263:373-375(1990) [PubMed: 2335242] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30. Tissue: Liver.
[4]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 63-82 AND 97-107, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M29859 mRNA. Translation: AAA41943.1. D90265 mRNA. Translation: BAA14312.1. BC062233 mRNA. Translation: AAH62233.1.
IPI	IPI00191748.
PIR	SNRTC2. A32968.
RefSeq	NP_058974.1.
UniGene	Rn.2668
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P40302.
SMR	P18420. Positions 4-241.
ModBase	Search...
Protein-protein interaction databases
STRING	P18420.
Protein family/group databases
MEROPS	T01.976.
Proteomic databases
PRIDE	P18420.
Genome annotation databases
Ensembl	ENSRNOT00000015946; ENSRNOP00000015946; ENSRNOG00000011745; Rattus norvegicus. [Genome view]
GeneID	29668.
KEGG	rno:29668.
NMPDR	fig|10116.3.peg.2814.
UCSC	NM_017278. rat.
Organism-specific databases
CTD	29668.
RGD	61841. Psma1.
Phylogenomic databases
HOVERGEN	P18420.
Enzyme and pathway databases
BRENDA	3.4.25.1. 248.
Gene expression databases
ArrayExpress	P18420.
Genevestigator	P18420.
GermOnline	ENSRNOG00000011745. Rattus norvegicus.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609983.

Entry information

Entry name

PSA1_RAT

Accession

Primary (citable) accession number: P18420

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 1, 1991
Last modified:	October 13, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)