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Protein

Proteasome subunit alpha type-1

Gene

Psma1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome. Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  1. lipopolysaccharide binding Source: Ensembl
  2. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. immune system process Source: UniProtKB-KW
  2. negative regulation of inflammatory response to antigenic stimulus Source: Ensembl
  3. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Immunity

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_198391. Asymmetric localization of PCP proteins.
REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_199197. ER-Phagosome pathway.
REACT_199247. Activation of NF-kappaB in B cells.
REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_206488. degradation of DVL.
REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
REACT_211117. Orc1 removal from chromatin.
REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
REACT_220232. Regulation of ornithine decarboxylase (ODC).
REACT_227706. degradation of AXIN.
REACT_235738. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_236654. Hedgehog ligand biogenesis.
REACT_240793. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_244609. SCF-beta-TrCP mediated degradation of Emi1.
REACT_245329. APC/C:Cdc20 mediated degradation of Securin.
REACT_246662. SCF(Skp2)-mediated degradation of p27/p21.
REACT_247625. Ubiquitin-dependent degradation of Cyclin D1.
REACT_252335. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_255131. Hh ligand biogenesis disease.
REACT_257365. Degradation of beta-catenin by the destruction complex.
REACT_259430. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_269272. Degradation of GLI1 by the proteasome.
REACT_269753. GLI3 is processed to GLI3R by the proteasome.
REACT_270919. Degradation of GLI2 by the proteasome.

Protein family/group databases

MEROPSiT01.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-1 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C2
Multicatalytic endopeptidase complex subunit C2
Proteasome component C2
Proteasome nu chain
Gene namesi
Name:Psma1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi61841. Psma1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. proteasome core complex Source: UniProtKB
  4. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Proteasome subunit alpha type-1PRO_0000124064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Glycosylationi110 – 1101O-linked (GlcNAc)By similarity
Cross-linki115 – 115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki208 – 208Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Proteolytically cleaved from a C-terminal extension in the course of the conversion of the proteasome from its latent form into its active form.

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP18420.
PRIDEiP18420.

2D gel databases

World-2DPAGE0004:P18420.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

GenevestigatoriP18420.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with NOTCH3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248289. 1 interaction.
IntActiP18420. 1 interaction.
STRINGi10116.ENSRNOP00000015946.

Structurei

3D structure databases

ProteinModelPortaliP18420.
SMRiP18420. Positions 4-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00550000074855.
HOGENOMiHOG000091080.
HOVERGENiHBG105373.
InParanoidiP18420.
KOiK02725.
OMAiFMKQQCL.
OrthoDBiEOG7WQ7T1.
PhylomeDBiP18420.
TreeFamiTF106206.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK
60 70 80 90 100
RAQSELAAHQ KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFD
110 120 130 140 150
RPLPVSRLVS LIGSKTQIPT QRYGRRPYGV GLLIAGYDDM GPHVFQTCPS
160 170 180 190 200
ANYFDCRAMS IGARSQSART YLERHMSEFM QCNLDELVKH GLRALRETLP
210 220 230 240 250
AEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLDGLEERP QRKAQPSQAA
260
DEPAEKADEP MEH
Length:263
Mass (Da):29,518
Last modified:February 1, 1991 - v2
Checksum:i68660696B43A4051
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29859 mRNA. Translation: AAA41943.1.
D90265 mRNA. Translation: BAA14312.1.
BC062233 mRNA. Translation: AAH62233.1.
PIRiA32968. SNRTC2.
RefSeqiNP_058974.1. NM_017278.1.
UniGeneiRn.2668.

Genome annotation databases

EnsembliENSRNOT00000015946; ENSRNOP00000015946; ENSRNOG00000011745.
GeneIDi29668.
KEGGirno:29668.
UCSCiRGD:61841. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29859 mRNA. Translation: AAA41943.1.
D90265 mRNA. Translation: BAA14312.1.
BC062233 mRNA. Translation: AAH62233.1.
PIRiA32968. SNRTC2.
RefSeqiNP_058974.1. NM_017278.1.
UniGeneiRn.2668.

3D structure databases

ProteinModelPortaliP18420.
SMRiP18420. Positions 4-241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248289. 1 interaction.
IntActiP18420. 1 interaction.
STRINGi10116.ENSRNOP00000015946.

Protein family/group databases

MEROPSiT01.976.

2D gel databases

World-2DPAGE0004:P18420.

Proteomic databases

PaxDbiP18420.
PRIDEiP18420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015946; ENSRNOP00000015946; ENSRNOG00000011745.
GeneIDi29668.
KEGGirno:29668.
UCSCiRGD:61841. rat.

Organism-specific databases

CTDi5682.
RGDi61841. Psma1.

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00550000074855.
HOGENOMiHOG000091080.
HOVERGENiHBG105373.
InParanoidiP18420.
KOiK02725.
OMAiFMKQQCL.
OrthoDBiEOG7WQ7T1.
PhylomeDBiP18420.
TreeFamiTF106206.

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_198391. Asymmetric localization of PCP proteins.
REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_199197. ER-Phagosome pathway.
REACT_199247. Activation of NF-kappaB in B cells.
REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_206488. degradation of DVL.
REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
REACT_211117. Orc1 removal from chromatin.
REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
REACT_220232. Regulation of ornithine decarboxylase (ODC).
REACT_227706. degradation of AXIN.
REACT_235738. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_236654. Hedgehog ligand biogenesis.
REACT_240793. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_244609. SCF-beta-TrCP mediated degradation of Emi1.
REACT_245329. APC/C:Cdc20 mediated degradation of Securin.
REACT_246662. SCF(Skp2)-mediated degradation of p27/p21.
REACT_247625. Ubiquitin-dependent degradation of Cyclin D1.
REACT_252335. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_255131. Hh ligand biogenesis disease.
REACT_257365. Degradation of beta-catenin by the destruction complex.
REACT_259430. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_269272. Degradation of GLI1 by the proteasome.
REACT_269753. GLI3 is processed to GLI3R by the proteasome.
REACT_270919. Degradation of GLI2 by the proteasome.

Miscellaneous databases

NextBioi609983.
PROiP18420.

Gene expression databases

GenevestigatoriP18420.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of cDNA for proteasomes (multicatalytic proteinase complexes) from rat liver: primary structure of the largest component (C2)."
    Fujiwara T., Tanaka K., Kumatori A., Shin S., Yoshimura T., Ichihara A., Tokunaga F., Aruga R., Iwanaga S., Kakizuka A., Nakanishi S.
    Biochemistry 28:7332-7340(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "The NH2-terminal residues of rat liver proteasome (multicatalytic proteinase complex) subunits, C2, C3 and C8, are N alpha-acetylated."
    Tokunaga F., Aruga R., Iwanaga S., Tanaka K., Ichihara A., Takao T., Shimonishi Y.
    FEBS Lett. 263:373-375(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-30, ACETYLATION AT MET-1.
    Tissue: Liver.
  4. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 63-82 AND 97-107, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiPSA1_RAT
AccessioniPrimary (citable) accession number: P18420
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1991
Last modified: March 4, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.