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Protein

Strictosidine synthase

Gene

STR1

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.

Catalytic activityi

3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

Pathwayi: 3alpha(S)-strictosidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 3alpha(S)-strictosidine from secologanin and tryptamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Strictosidine synthase (STR1)
This subpathway is part of the pathway 3alpha(S)-strictosidine biosynthesis, which is itself part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3alpha(S)-strictosidine from secologanin and tryptamine, the pathway 3alpha(S)-strictosidine biosynthesis and in Alkaloid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Alkaloid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11582.
BRENDAi4.3.3.2. 1211.
UniPathwayiUPA00311; UER00447.

Names & Taxonomyi

Protein namesi
Recommended name:
Strictosidine synthase (EC:4.3.3.2)
Gene namesi
Name:STR1
Synonyms:SSS
OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Taxonomic identifieri4058 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4369.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 311 PublicationAdd BLAST31
ChainiPRO_000003333232 – 352Strictosidine synthaseAdd BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1
Glycosylationi187N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP18417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the strictosidine synthase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426. PTHR10426. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18417-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFSESKSM MAVFFMFFLL LLSSSSSSSS SSPILKKIFI ESPSYAPNAF
60 70 80 90 100
TFDSTDKGFY TSVQDGRVIK YEGPNSGFTD FAYASPFWNK AFCENSTDPE
110 120 130 140 150
KRPLCGRTYD ISYDYKNSQM YIVDGHYHLC VVGKEGGYAT QLATSVQGVP
160 170 180 190 200
FKWLYAVTVD QRTGIVYFTD VSSIHDDSPE GVEEIMNTSD RTGRLMKYDP
210 220 230 240 250
STKETTLLLK ELHVPGGAEI SADGSFVVVA EFLSNRIVKY WLEGPKKGSA
260 270 280 290 300
EFLVTIPNPG NIKRNSDGHF WVSSSEELDG GQHGRVVSRG IKFDGFGNIL
310 320 330 340 350
QVIPLPPPYE GEHFEQIQEH DGLLYIGSLF HSSVGILVYD DHDNKGNSYV

SS
Length:352
Mass (Da):39,094
Last modified:April 1, 1993 - v2
Checksum:i1D6DD289A00272B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti285R → S in CAA37671 (PubMed:2395663).Curated1
Sequence conflicti330F → S in CAA37671 (PubMed:2395663).Curated1
Sequence conflicti352S → QLVIN in CAA37671 (PubMed:2395663).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61932 mRNA. Translation: CAA43936.1.
Y10182 Genomic DNA. Translation: CAA71255.1.
X53602 mRNA. Translation: CAA37671.1.
PIRiS22464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61932 mRNA. Translation: CAA43936.1.
Y10182 Genomic DNA. Translation: CAA71255.1.
X53602 mRNA. Translation: CAA37671.1.
PIRiS22464.

3D structure databases

ProteinModelPortaliP18417.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

ChEMBLiCHEMBL4369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00311; UER00447.
BioCyciMetaCyc:MONOMER-11582.
BRENDAi4.3.3.2. 1211.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426. PTHR10426. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTSY_CATRO
AccessioniPrimary (citable) accession number: P18417
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: April 1, 1993
Last modified: October 5, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.