Reviewed, UniProtKB/Swiss-Prot P18417 (STSY_CATRO)

Names and origin

Protein names

Recommended name:
    Strictosidine synthase
    EC=4.3.3.2

Gene names

Name:	STR1
Synonyms:	SSS

Organism

Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)

Taxonomic identifier

4058 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthus

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.
Catalytic activity	3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.
Pathway	Alkaloid biosynthesis; 3alpha(S)-strictosidine biosynthesis; 3alpha(S)-strictosidine from secologanin and tryptamine: step 1/1.
Subunit structure	Monomer.
Subcellular location	Vacuole.
Sequence similarities	Belongs to the strictosidine synthase family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Cellular component	Vacuole
Domain	Signal
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	strictosidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Ref.1
	Chain	32 – 352	321	Strictosidine synthase		PRO_0000033332
Amino acid modifications
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	285	1	R → S in CAA37671. Ref.3
	Sequence conflict	330	1	F → S in CAA37671. Ref.3
	Sequence conflict	352	1	S → QLVIN in CAA37671. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P18417-1 [UniParc]. Last modified April 1, 1993. Version 2. Checksum: 1D6DD289A00272B8	FASTA	352	39,094
	10 20 30 40 50 60 MANFSESKSM MAVFFMFFLL LLSSSSSSSS SSPILKKIFI ESPSYAPNAF TFDSTDKGFY 70 80 90 100 110 120 TSVQDGRVIK YEGPNSGFTD FAYASPFWNK AFCENSTDPE KRPLCGRTYD ISYDYKNSQM 130 140 150 160 170 180 YIVDGHYHLC VVGKEGGYAT QLATSVQGVP FKWLYAVTVD QRTGIVYFTD VSSIHDDSPE 190 200 210 220 230 240 GVEEIMNTSD RTGRLMKYDP STKETTLLLK ELHVPGGAEI SADGSFVVVA EFLSNRIVKY 250 260 270 280 290 300 WLEGPKKGSA EFLVTIPNPG NIKRNSDGHF WVSSSEELDG GQHGRVVSRG IKFDGFGNIL 310 320 330 340 350 QVIPLPPPYE GEHFEQIQEH DGLLYIGSLF HSSVGILVYD DHDNKGNSYV SS

References

[1]	"Coordinated regulation of two indole alkaloid biosynthetic genes from Catharanthus roseus by auxin and elicitors." Pasquali G., Goddijn O.J.M., de Waal A., Verpoorte R., Schilperoort R.A., Hoge J.H.C., Memelink J. Plant Mol. Biol. 18:1121-1131(1992) [PubMed: 1600148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-52. Strain: cv. G. Don.
[2]	Pasquali G., Erven A., Menke F., Memelink J. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Morning mist.
[3]	"Nucleotide sequence of a cDNA encoding the vacuolar protein strictosidine synthase from Catharanthus roseus." McKnight T.D., Roessner C.A., Devagupta R., Scott A.I., Nessler C.L. Nucleic Acids Res. 18:4939-4939(1990) [PubMed: 2395663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-352.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61932 mRNA. Translation: CAA43936.1. Y10182 Genomic DNA. Translation: CAA71255.1. X53602 mRNA. Translation: CAA37671.1.
PIR	S22464.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11582.
BRENDA	4.3.3.2. 20471.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR018119. Strictosidine_synth_cons-reg. IPR004141. Strictosidine_synthase. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
PANTHER	PTHR10426. Str_synth. 1 hit.
Pfam	PF03088. Str_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STSY_CATRO

Accession

Primary (citable) accession number: P18417

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	April 1, 1993
Last modified:	September 22, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)