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P18417

- STSY_CATRO

UniProt

P18417 - STSY_CATRO

Protein

Strictosidine synthase

Gene

STR1

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.

    Catalytic activityi

    3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

    Pathwayi

    GO - Molecular functioni

    1. strictosidine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. alkaloid metabolic process Source: UniProtKB-KW
    2. biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Alkaloid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-11582.
    UniPathwayiUPA00311; UER00447.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Strictosidine synthase (EC:4.3.3.2)
    Gene namesi
    Name:STR1
    Synonyms:SSS
    OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
    Taxonomic identifieri4058 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

    Subcellular locationi

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 31311 PublicationAdd
    BLAST
    Chaini32 – 352321Strictosidine synthasePRO_0000033332Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP18417.
    SMRiP18417. Positions 33-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the strictosidine synthase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR018119. Strictosidine_synth_cons-reg.
    IPR004141. Strictosidine_synthase.
    [Graphical view]
    PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
    PfamiPF03088. Str_synth. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18417-1 [UniParc]FASTAAdd to Basket

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    MANFSESKSM MAVFFMFFLL LLSSSSSSSS SSPILKKIFI ESPSYAPNAF    50
    TFDSTDKGFY TSVQDGRVIK YEGPNSGFTD FAYASPFWNK AFCENSTDPE 100
    KRPLCGRTYD ISYDYKNSQM YIVDGHYHLC VVGKEGGYAT QLATSVQGVP 150
    FKWLYAVTVD QRTGIVYFTD VSSIHDDSPE GVEEIMNTSD RTGRLMKYDP 200
    STKETTLLLK ELHVPGGAEI SADGSFVVVA EFLSNRIVKY WLEGPKKGSA 250
    EFLVTIPNPG NIKRNSDGHF WVSSSEELDG GQHGRVVSRG IKFDGFGNIL 300
    QVIPLPPPYE GEHFEQIQEH DGLLYIGSLF HSSVGILVYD DHDNKGNSYV 350
    SS 352
    Length:352
    Mass (Da):39,094
    Last modified:April 1, 1993 - v2
    Checksum:i1D6DD289A00272B8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti285 – 2851R → S in CAA37671. (PubMed:2395663)Curated
    Sequence conflicti330 – 3301F → S in CAA37671. (PubMed:2395663)Curated
    Sequence conflicti352 – 3521S → QLVIN in CAA37671. (PubMed:2395663)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61932 mRNA. Translation: CAA43936.1.
    Y10182 Genomic DNA. Translation: CAA71255.1.
    X53602 mRNA. Translation: CAA37671.1.
    PIRiS22464.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61932 mRNA. Translation: CAA43936.1 .
    Y10182 Genomic DNA. Translation: CAA71255.1 .
    X53602 mRNA. Translation: CAA37671.1 .
    PIRi S22464.

    3D structure databases

    ProteinModelPortali P18417.
    SMRi P18417. Positions 33-339.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL4369.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00311 ; UER00447 .
    BioCyci MetaCyc:MONOMER-11582.

    Family and domain databases

    Gene3Di 2.120.10.30. 1 hit.
    InterProi IPR011042. 6-blade_b-propeller_TolB-like.
    IPR018119. Strictosidine_synth_cons-reg.
    IPR004141. Strictosidine_synthase.
    [Graphical view ]
    PANTHERi PTHR10426:SF22. PTHR10426:SF22. 1 hit.
    Pfami PF03088. Str_synth. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Coordinated regulation of two indole alkaloid biosynthetic genes from Catharanthus roseus by auxin and elicitors."
      Pasquali G., Goddijn O.J.M., de Waal A., Verpoorte R., Schilperoort R.A., Hoge J.H.C., Memelink J.
      Plant Mol. Biol. 18:1121-1131(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-52.
      Strain: cv. G. Don.
    2. Pasquali G., Erven A., Menke F., Memelink J.
      Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Morning mist.
    3. "Nucleotide sequence of a cDNA encoding the vacuolar protein strictosidine synthase from Catharanthus roseus."
      McKnight T.D., Roessner C.A., Devagupta R., Scott A.I., Nessler C.L.
      Nucleic Acids Res. 18:4939-4939(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-352.

    Entry informationi

    Entry nameiSTSY_CATRO
    AccessioniPrimary (citable) accession number: P18417
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3