P18410 (SPO7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sporulation-specific protein SPO7

Gene names

Name:	SPO7
Ordered Locus Names:	YAL009W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for sporulation. Essential for the formation of a spherical nucleus and meiotic division. Probable regulatory component of the NEM1-SPO7 complex which acts as a phosphatase and dephosphorylates PAH1/SMP2. Ref.6 Ref.8
Subunit structure	Component of the NEM1-SPO7 complex. Ref.6
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein Ref.6.
Miscellaneous	Present with 861 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Lipid metabolism Sporulation
Cellular component	Endoplasmic reticulum Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of phospholipid biosynthetic process Inferred from genetic interaction Ref.8. Source: SGD nuclear envelope organization Inferred from mutant phenotype Ref.6. Source: SGD sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Nem1-Spo7 phosphatase complex Inferred from physical interaction Ref.6. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from direct assay Ref.6. Source: SGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NEM1	P38757	4	EBI-17857,EBI-24435

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Sporulation-specific protein SPO7		PRO_0000072145
Regions
	Topological domain	1 – 72	72	Cytoplasmic Potential
	Transmembrane	73 – 93	21	Helical; Potential
	Topological domain	94 – 106	13	Extracellular Potential
	Transmembrane	107 – 127	21	Helical; Potential
	Topological domain	128 – 259	132	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P18410 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 480A73B62EA85E18	FASTA	259	30,114
	10 20 30 40 50 60 MEPESIGDVG NHAQDDSASI VSGPRRRSTS KTSSAKNIRN SSNISPASMI FRNLLILEDD 70 80 90 100 110 120 LRRQAHEQKI LKWQFTLFLA SMAGVGAFTF YELYFTSDYV KGLHRVILQF TLSFISITVV 130 140 150 160 170 180 LFHISGQYRR TIVIPRRFFT STNKGIRQFN VKLVKVQSTW DEKYTDSVRF VSRTIAYCNI 190 200 210 220 230 240 YCLKKFLWLK DDNAIVKFWK SVTIQSQPRI GAVDVKLVLN PRAFSAEIRE GWEIYRDEFW 250 AREGARRRKQ AHELRPKSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation, characterization and regulation of the SPO7 sporulation gene." Whyte W., Koepp L.H., Lamb J., Crowley J.C., Kaback D.B. Gene 95:65-72(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"A novel complex of membrane proteins required for formation of a spherical nucleus." Siniossoglou S., Santos-Rosa H., Rappsilber J., Mann M., Hurt E. EMBO J. 17:6449-6464(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE NEM1-SPO7 COMPLEX, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth." Santos-Rosa H., Leung J., Grimsey N., Peak-Chew S., Siniossoglou S. EMBO J. 24:1931-1941(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE NEM1-SPO7 COMPLEX.
[9]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36073 Genomic DNA. Translation: AAA35073.1. L22015 Genomic DNA. Translation: AAC04949.1. AY558585 Genomic DNA. Translation: AAS56911.1. BK006935 Genomic DNA. Translation: DAA06979.1.
PIR	JN0099.
RefSeq	NP_009393.1. NM_001178154.1.
3D structure databases
ProteinModelPortal	P18410.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2441N.
IntAct	P18410. 17 interactions.
MINT	MINT-617898.
STRING	4932.YAL009W.
Proteomic databases
PaxDb	P18410.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL009W; YAL009W; YAL009W.
GeneID	851224.
KEGG	sce:YAL009W.
Organism-specific databases
CYGD	YAL009w.
SGD	S000000007. SPO7.
Phylogenomic databases
eggNOG	NOG71981.
HOGENOM	HOG000111152.
OMA	AEIREGW.
OrthoDB	EOG4WM84H.
Gene expression databases
Genevestigator	P18410.
GermOnline	YAL009W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005605. Spore/nuc_morph_Spo7. [Graphical view]
Pfam	PF03907. Spo7. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968127.

Entry information

Entry name

SPO7_YEAST

Accession

Primary (citable) accession number: P18410
Secondary accession number(s): D6VPK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program