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Protein

Sporulation-specific protein SPO7

Gene

SPO7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for sporulation. Essential for the formation of a spherical nucleus and meiotic division. Probable regulatory component of the NEM1-SPO7 complex which acts as a phosphatase and dephosphorylates PAH1/SMP2.2 Publications

GO - Biological processi

  • lipid metabolic process Source: UniProtKB-KW
  • negative regulation of phosphatidate phosphatase activity Source: SGD
  • negative regulation of phospholipid biosynthetic process Source: SGD
  • nuclear envelope organization Source: SGD
  • protein dephosphorylation Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Lipid metabolism, Sporulation

Enzyme and pathway databases

BioCyciYEAST:G3O-28822-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation-specific protein SPO7
Gene namesi
Name:SPO7
Ordered Locus Names:YAL009W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome I

Organism-specific databases

CYGDiYAL009w.
EuPathDBiFungiDB:YAL009W.
SGDiS000000007. SPO7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7272CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei73 – 9321HelicalSequence AnalysisAdd
BLAST
Topological domaini94 – 10613ExtracellularSequence AnalysisAdd
BLAST
Transmembranei107 – 12721HelicalSequence AnalysisAdd
BLAST
Topological domaini128 – 259132CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
  • Nem1-Spo7 phosphatase complex Source: SGD
  • nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Sporulation-specific protein SPO7PRO_0000072145Add
BLAST

Proteomic databases

MaxQBiP18410.
PaxDbiP18410.

Interactioni

Subunit structurei

Component of the NEM1-SPO7 complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NEM1P387574EBI-17857,EBI-24435

Protein-protein interaction databases

BioGridi31757. 78 interactions.
DIPiDIP-2441N.
IntActiP18410. 18 interactions.
MINTiMINT-617898.
STRINGi4932.YAL009W.

Structurei

3D structure databases

ProteinModelPortaliP18410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG71981.
HOGENOMiHOG000111152.
InParanoidiP18410.
OMAiWEIYRDE.
OrthoDBiEOG71CFX7.

Family and domain databases

InterProiIPR005605. Spore/nuc_morph_Spo7.
[Graphical view]
PfamiPF03907. Spo7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18410-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPESIGDVG NHAQDDSASI VSGPRRRSTS KTSSAKNIRN SSNISPASMI
60 70 80 90 100
FRNLLILEDD LRRQAHEQKI LKWQFTLFLA SMAGVGAFTF YELYFTSDYV
110 120 130 140 150
KGLHRVILQF TLSFISITVV LFHISGQYRR TIVIPRRFFT STNKGIRQFN
160 170 180 190 200
VKLVKVQSTW DEKYTDSVRF VSRTIAYCNI YCLKKFLWLK DDNAIVKFWK
210 220 230 240 250
SVTIQSQPRI GAVDVKLVLN PRAFSAEIRE GWEIYRDEFW AREGARRRKQ

AHELRPKSE
Length:259
Mass (Da):30,114
Last modified:November 1, 1990 - v1
Checksum:i480A73B62EA85E18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36073 Genomic DNA. Translation: AAA35073.1.
L22015 Genomic DNA. Translation: AAC04949.1.
AY558585 Genomic DNA. Translation: AAS56911.1.
BK006935 Genomic DNA. Translation: DAA06979.1.
PIRiJN0099.
RefSeqiNP_009393.1. NM_001178154.1.

Genome annotation databases

EnsemblFungiiYAL009W; YAL009W; YAL009W.
GeneIDi851224.
KEGGisce:YAL009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36073 Genomic DNA. Translation: AAA35073.1.
L22015 Genomic DNA. Translation: AAC04949.1.
AY558585 Genomic DNA. Translation: AAS56911.1.
BK006935 Genomic DNA. Translation: DAA06979.1.
PIRiJN0099.
RefSeqiNP_009393.1. NM_001178154.1.

3D structure databases

ProteinModelPortaliP18410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31757. 78 interactions.
DIPiDIP-2441N.
IntActiP18410. 18 interactions.
MINTiMINT-617898.
STRINGi4932.YAL009W.

Proteomic databases

MaxQBiP18410.
PaxDbiP18410.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL009W; YAL009W; YAL009W.
GeneIDi851224.
KEGGisce:YAL009W.

Organism-specific databases

CYGDiYAL009w.
EuPathDBiFungiDB:YAL009W.
SGDiS000000007. SPO7.

Phylogenomic databases

eggNOGiNOG71981.
HOGENOMiHOG000111152.
InParanoidiP18410.
OMAiWEIYRDE.
OrthoDBiEOG71CFX7.

Enzyme and pathway databases

BioCyciYEAST:G3O-28822-MONOMER.

Miscellaneous databases

NextBioi968127.
PROiP18410.

Family and domain databases

InterProiIPR005605. Spore/nuc_morph_Spo7.
[Graphical view]
PfamiPF03907. Spo7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation, characterization and regulation of the SPO7 sporulation gene."
    Whyte W., Koepp L.H., Lamb J., Crowley J.C., Kaback D.B.
    Gene 95:65-72(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
    Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
    Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "A novel complex of membrane proteins required for formation of a spherical nucleus."
    Siniossoglou S., Santos-Rosa H., Rappsilber J., Mann M., Hurt E.
    EMBO J. 17:6449-6464(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE NEM1-SPO7 COMPLEX, SUBCELLULAR LOCATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth."
    Santos-Rosa H., Leung J., Grimsey N., Peak-Chew S., Siniossoglou S.
    EMBO J. 24:1931-1941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NEM1-SPO7 COMPLEX.
  9. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiSPO7_YEAST
AccessioniPrimary (citable) accession number: P18410
Secondary accession number(s): D6VPK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 24, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 861 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.