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P18408

- MET16_YEAST

UniProt

P18408 - MET16_YEAST

Protein

Phosphoadenosine phosphosulfate reductase

Gene

MET16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    The NADP dependent reduction of PAPS into sulfite involves thioredoxin which probably plays the role of a thiol carrier.

    Catalytic activityi

    Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.

    Pathwayi

    GO - Molecular functioni

    1. phosphoadenylyl-sulfate reductase (thioredoxin) activity Source: SGD

    GO - Biological processi

    1. cysteine biosynthetic process Source: UniProtKB-KW
    2. hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
    3. methionine biosynthetic process Source: UniProtKB-KW
    4. sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Source: SGD
    5. sulfur amino acid metabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-384.
    YEAST:YPR167C-MONOMER.
    UniPathwayiUPA00140; UER00206.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoadenosine phosphosulfate reductase (EC:1.8.4.8)
    Alternative name(s):
    3'-phosphoadenylylsulfate reductase
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    Gene namesi
    Name:MET16
    Ordered Locus Names:YPR167C
    ORF Names:P9325.8
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR167c.
    SGDiS000006371. MET16.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Phosphoadenosine phosphosulfate reductasePRO_0000100662Add
    BLAST

    Proteomic databases

    MaxQBiP18408.
    PaxDbiP18408.
    PeptideAtlasiP18408.

    Expressioni

    Gene expression databases

    GenevestigatoriP18408.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TRX1P222171EBI-11481,EBI-19607

    Protein-protein interaction databases

    BioGridi36340. 49 interactions.
    DIPiDIP-6611N.
    MINTiMINT-677033.
    STRINGi4932.YPR167C.

    Structurei

    Secondary structure

    1
    261
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 53
    Helixi7 – 93
    Beta strandi11 – 133
    Helixi14 – 2411
    Helixi30 – 4011
    Beta strandi42 – 476
    Helixi52 – 6413
    Turni65 – 673
    Beta strandi72 – 765
    Helixi83 – 9614
    Helixi98 – 1003
    Beta strandi105 – 1073
    Helixi115 – 1228
    Helixi126 – 1294
    Helixi131 – 1388
    Helixi140 – 14910
    Beta strandi153 – 1564
    Helixi161 – 1633
    Helixi165 – 1695
    Beta strandi172 – 1765
    Turni177 – 1804
    Beta strandi181 – 1844
    Turni186 – 1894
    Helixi192 – 20211
    Helixi208 – 2125
    Helixi220 – 2223
    Turni232 – 2365
    Turni246 – 2483
    Helixi251 – 2533

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2OQ2X-ray2.10A/B/C/D1-261[»]
    ProteinModelPortaliP18408.
    SMRiP18408. Positions 1-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18408.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PAPS reductase family. CysH subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0175.
    HOGENOMiHOG000249397.
    KOiK00390.
    OMAiIGDVHST.
    OrthoDBiEOG7P2Z3B.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    InterProiIPR004511. PAPS/APS_Rdtase.
    IPR002500. PAPS_reduct.
    IPR011800. PAPS_reductase_CysH.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF01507. PAPS_reduct. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
    TIGRFAMsiTIGR00434. cysH. 1 hit.
    TIGR02057. PAPS_reductase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P18408-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTYHLNNDI IVTQEQLDHW NEQLIKLETP QEIIAWSIVT FPHLFQTTAF    50
    GLTGLVTIDM LSKLSEKYYM PELLFIDTLH HFPQTLTLKN EIEKKYYQPK 100
    NQTIHVYKPD GCESEADFAS KYGDFLWEKD DDKYDYLAKV EPAHRAYKEL 150
    HISAVFTGRR KSQGSARSQL SIIEIDELNG ILKINPLINW TFEQVKQYID 200
    ANNVPYNELL DLGYRSIGDY HSTQPVKEGE DERAGRWKGK AKTECGIHEA 250
    SRFAQFLKQD A 261
    Length:261
    Mass (Da):30,380
    Last modified:November 1, 1997 - v2
    Checksum:i202AF2C20829513C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti242 – 26120KTECG…LKQDA → RPSVEFMKPADSRNF in AAA34774. (PubMed:2203779)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05591 Genomic DNA. Translation: AAA34774.1.
    U25840 Genomic DNA. Translation: AAB68154.1.
    AY693236 Genomic DNA. Translation: AAT93255.1.
    BK006949 Genomic DNA. Translation: DAA11584.1.
    PIRiS59826.
    RefSeqiNP_015493.1. NM_001184264.1.

    Genome annotation databases

    EnsemblFungiiYPR167C; YPR167C; YPR167C.
    GeneIDi856296.
    KEGGisce:YPR167C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05591 Genomic DNA. Translation: AAA34774.1 .
    U25840 Genomic DNA. Translation: AAB68154.1 .
    AY693236 Genomic DNA. Translation: AAT93255.1 .
    BK006949 Genomic DNA. Translation: DAA11584.1 .
    PIRi S59826.
    RefSeqi NP_015493.1. NM_001184264.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2OQ2 X-ray 2.10 A/B/C/D 1-261 [» ]
    ProteinModelPortali P18408.
    SMRi P18408. Positions 1-257.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36340. 49 interactions.
    DIPi DIP-6611N.
    MINTi MINT-677033.
    STRINGi 4932.YPR167C.

    Proteomic databases

    MaxQBi P18408.
    PaxDbi P18408.
    PeptideAtlasi P18408.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR167C ; YPR167C ; YPR167C .
    GeneIDi 856296.
    KEGGi sce:YPR167C.

    Organism-specific databases

    CYGDi YPR167c.
    SGDi S000006371. MET16.

    Phylogenomic databases

    eggNOGi COG0175.
    HOGENOMi HOG000249397.
    KOi K00390.
    OMAi IGDVHST.
    OrthoDBi EOG7P2Z3B.

    Enzyme and pathway databases

    UniPathwayi UPA00140 ; UER00206 .
    BioCyci MetaCyc:MONOMER-384.
    YEAST:YPR167C-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P18408.
    NextBioi 981647.
    PROi P18408.

    Gene expression databases

    Genevestigatori P18408.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    InterProi IPR004511. PAPS/APS_Rdtase.
    IPR002500. PAPS_reduct.
    IPR011800. PAPS_reductase_CysH.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF01507. PAPS_reduct. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000857. PAPS_reductase. 1 hit.
    TIGRFAMsi TIGR00434. cysH. 1 hit.
    TIGR02057. PAPS_reductase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene-enzyme relationship in the sulfate assimilation pathway of Saccharomyces cerevisiae. Study of the 3'-phosphoadenylylsulfate reductase structural gene."
      Thomas D., Barbey R., Surdin-Kerjan Y.
      J. Biol. Chem. 265:15518-15524(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMET16_YEAST
    AccessioniPrimary (citable) accession number: P18408
    Secondary accession number(s): D6W4G8, Q06212
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 217 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3