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Protein

Phosphoadenosine phosphosulfate reductase

Gene

MET16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The NADP dependent reduction of PAPS into sulfite involves thioredoxin which probably plays the role of a thiol carrier.

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase (MET3)
  2. Adenylyl-sulfate kinase (MET14)
  3. Phosphoadenosine phosphosulfate reductase (MET16)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:YPR167C-MONOMER
YEAST:YPR167C-MONOMER
UniPathwayiUPA00140; UER00206

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoadenosine phosphosulfate reductase (EC:1.8.4.8)
Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAdoPS reductase
Gene namesi
Name:MET16
Ordered Locus Names:YPR167C
ORF Names:P9325.8
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR167C
SGDiS000006371 MET16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001006621 – 261Phosphoadenosine phosphosulfate reductaseAdd BLAST261

Proteomic databases

MaxQBiP18408
PaxDbiP18408
PRIDEiP18408

Interactioni

Protein-protein interaction databases

BioGridi36340, 99 interactors
DIPiDIP-6611N
IntActiP18408, 1 interactor
STRINGi4932.YPR167C

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Helixi7 – 9Combined sources3
Beta strandi11 – 13Combined sources3
Helixi14 – 24Combined sources11
Helixi30 – 40Combined sources11
Beta strandi42 – 47Combined sources6
Helixi52 – 64Combined sources13
Turni65 – 67Combined sources3
Beta strandi72 – 76Combined sources5
Helixi83 – 96Combined sources14
Helixi98 – 100Combined sources3
Beta strandi105 – 107Combined sources3
Helixi115 – 122Combined sources8
Helixi126 – 129Combined sources4
Helixi131 – 138Combined sources8
Helixi140 – 149Combined sources10
Beta strandi153 – 156Combined sources4
Helixi161 – 163Combined sources3
Helixi165 – 169Combined sources5
Beta strandi172 – 176Combined sources5
Turni177 – 180Combined sources4
Beta strandi181 – 184Combined sources4
Turni186 – 189Combined sources4
Helixi192 – 202Combined sources11
Helixi208 – 212Combined sources5
Helixi220 – 222Combined sources3
Turni232 – 236Combined sources5
Turni246 – 248Combined sources3
Helixi251 – 253Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OQ2X-ray2.10A/B/C/D1-261[»]
ProteinModelPortaliP18408
SMRiP18408
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18408

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysH subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000249397
InParanoidiP18408
KOiK00390
OMAiVGDWHSS
OrthoDBiEOG092C4JOA

Family and domain databases

CDDicd01713 PAPS_reductase, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR004511 PAPS/APS_Rdtase
IPR002500 PAPS_reduct
IPR011800 PAPS_reductase_CysH
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01507 PAPS_reduct, 1 hit
PIRSFiPIRSF000857 PAPS_reductase, 1 hit
TIGRFAMsiTIGR00434 cysH, 1 hit
TIGR02057 PAPS_reductase, 1 hit

Sequencei

Sequence statusi: Complete.

P18408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTYHLNNDI IVTQEQLDHW NEQLIKLETP QEIIAWSIVT FPHLFQTTAF
60 70 80 90 100
GLTGLVTIDM LSKLSEKYYM PELLFIDTLH HFPQTLTLKN EIEKKYYQPK
110 120 130 140 150
NQTIHVYKPD GCESEADFAS KYGDFLWEKD DDKYDYLAKV EPAHRAYKEL
160 170 180 190 200
HISAVFTGRR KSQGSARSQL SIIEIDELNG ILKINPLINW TFEQVKQYID
210 220 230 240 250
ANNVPYNELL DLGYRSIGDY HSTQPVKEGE DERAGRWKGK AKTECGIHEA
260
SRFAQFLKQD A
Length:261
Mass (Da):30,380
Last modified:November 1, 1997 - v2
Checksum:i202AF2C20829513C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti242 – 261KTECG…LKQDA → RPSVEFMKPADSRNF in AAA34774 (PubMed:2203779).CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05591 Genomic DNA Translation: AAA34774.1
U25840 Genomic DNA Translation: AAB68154.1
AY693236 Genomic DNA Translation: AAT93255.1
BK006949 Genomic DNA Translation: DAA11584.1
PIRiS59826
RefSeqiNP_015493.1, NM_001184264.1

Genome annotation databases

EnsemblFungiiYPR167C; YPR167C; YPR167C
GeneIDi856296
KEGGisce:YPR167C

Similar proteinsi

Entry informationi

Entry nameiMET16_YEAST
AccessioniPrimary (citable) accession number: P18408
Secondary accession number(s): D6W4G8, Q06212
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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