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Protein

Phosphoadenosine phosphosulfate reductase

Gene

MET16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The NADP dependent reduction of PAPS into sulfite involves thioredoxin which probably plays the role of a thiol carrier.

Catalytic activityi

Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase (MET3)
  2. Adenylyl-sulfate kinase (MET14)
  3. Phosphoadenosine phosphosulfate reductase (MET16)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:YPR167C-MONOMER.
YEAST:YPR167C-MONOMER.
ReactomeiR-SCE-2408550. Metabolism of ingested H2SeO4 and H2SeO3 into H2Se.
UniPathwayiUPA00140; UER00206.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoadenosine phosphosulfate reductase (EC:1.8.4.8)
Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAdoPS reductase
Gene namesi
Name:MET16
Ordered Locus Names:YPR167C
ORF Names:P9325.8
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR167C.
SGDiS000006371. MET16.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001006621 – 261Phosphoadenosine phosphosulfate reductaseAdd BLAST261

Proteomic databases

MaxQBiP18408.
PRIDEiP18408.

Interactioni

Protein-protein interaction databases

BioGridi36340. 50 interactors.
DIPiDIP-6611N.
MINTiMINT-677033.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Helixi7 – 9Combined sources3
Beta strandi11 – 13Combined sources3
Helixi14 – 24Combined sources11
Helixi30 – 40Combined sources11
Beta strandi42 – 47Combined sources6
Helixi52 – 64Combined sources13
Turni65 – 67Combined sources3
Beta strandi72 – 76Combined sources5
Helixi83 – 96Combined sources14
Helixi98 – 100Combined sources3
Beta strandi105 – 107Combined sources3
Helixi115 – 122Combined sources8
Helixi126 – 129Combined sources4
Helixi131 – 138Combined sources8
Helixi140 – 149Combined sources10
Beta strandi153 – 156Combined sources4
Helixi161 – 163Combined sources3
Helixi165 – 169Combined sources5
Beta strandi172 – 176Combined sources5
Turni177 – 180Combined sources4
Beta strandi181 – 184Combined sources4
Turni186 – 189Combined sources4
Helixi192 – 202Combined sources11
Helixi208 – 212Combined sources5
Helixi220 – 222Combined sources3
Turni232 – 236Combined sources5
Turni246 – 248Combined sources3
Helixi251 – 253Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OQ2X-ray2.10A/B/C/D1-261[»]
ProteinModelPortaliP18408.
SMRiP18408.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18408.

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysH subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000249397.
InParanoidiP18408.
KOiK00390.
OMAiPRNKLLD.
OrthoDBiEOG092C4JOA.

Family and domain databases

CDDicd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.

Sequencei

Sequence statusi: Complete.

P18408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTYHLNNDI IVTQEQLDHW NEQLIKLETP QEIIAWSIVT FPHLFQTTAF
60 70 80 90 100
GLTGLVTIDM LSKLSEKYYM PELLFIDTLH HFPQTLTLKN EIEKKYYQPK
110 120 130 140 150
NQTIHVYKPD GCESEADFAS KYGDFLWEKD DDKYDYLAKV EPAHRAYKEL
160 170 180 190 200
HISAVFTGRR KSQGSARSQL SIIEIDELNG ILKINPLINW TFEQVKQYID
210 220 230 240 250
ANNVPYNELL DLGYRSIGDY HSTQPVKEGE DERAGRWKGK AKTECGIHEA
260
SRFAQFLKQD A
Length:261
Mass (Da):30,380
Last modified:November 1, 1997 - v2
Checksum:i202AF2C20829513C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti242 – 261KTECG…LKQDA → RPSVEFMKPADSRNF in AAA34774 (PubMed:2203779).CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05591 Genomic DNA. Translation: AAA34774.1.
U25840 Genomic DNA. Translation: AAB68154.1.
AY693236 Genomic DNA. Translation: AAT93255.1.
BK006949 Genomic DNA. Translation: DAA11584.1.
PIRiS59826.
RefSeqiNP_015493.1. NM_001184264.1.

Genome annotation databases

EnsemblFungiiYPR167C; YPR167C; YPR167C.
GeneIDi856296.
KEGGisce:YPR167C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05591 Genomic DNA. Translation: AAA34774.1.
U25840 Genomic DNA. Translation: AAB68154.1.
AY693236 Genomic DNA. Translation: AAT93255.1.
BK006949 Genomic DNA. Translation: DAA11584.1.
PIRiS59826.
RefSeqiNP_015493.1. NM_001184264.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OQ2X-ray2.10A/B/C/D1-261[»]
ProteinModelPortaliP18408.
SMRiP18408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36340. 50 interactors.
DIPiDIP-6611N.
MINTiMINT-677033.

Proteomic databases

MaxQBiP18408.
PRIDEiP18408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR167C; YPR167C; YPR167C.
GeneIDi856296.
KEGGisce:YPR167C.

Organism-specific databases

EuPathDBiFungiDB:YPR167C.
SGDiS000006371. MET16.

Phylogenomic databases

HOGENOMiHOG000249397.
InParanoidiP18408.
KOiK00390.
OMAiPRNKLLD.
OrthoDBiEOG092C4JOA.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00206.
BioCyciMetaCyc:YPR167C-MONOMER.
YEAST:YPR167C-MONOMER.
ReactomeiR-SCE-2408550. Metabolism of ingested H2SeO4 and H2SeO3 into H2Se.

Miscellaneous databases

EvolutionaryTraceiP18408.
PROiP18408.

Family and domain databases

CDDicd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMET16_YEAST
AccessioniPrimary (citable) accession number: P18408
Secondary accession number(s): D6W4G8, Q06212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.