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P18408

- MET16_YEAST

UniProt

P18408 - MET16_YEAST

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Protein

Phosphoadenosine phosphosulfate reductase

Gene

MET16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The NADP dependent reduction of PAPS into sulfite involves thioredoxin which probably plays the role of a thiol carrier.

Catalytic activityi

Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.

Pathwayi

GO - Molecular functioni

  1. phosphoadenylyl-sulfate reductase (thioredoxin) activity Source: SGD

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  3. methionine biosynthetic process Source: UniProtKB-KW
  4. sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Source: SGD
  5. sulfur amino acid metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-384.
YEAST:YPR167C-MONOMER.
UniPathwayiUPA00140; UER00206.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoadenosine phosphosulfate reductase (EC:1.8.4.8)
Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAdoPS reductase
Gene namesi
Name:MET16
Ordered Locus Names:YPR167C
ORF Names:P9325.8
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR167c.
SGDiS000006371. MET16.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Phosphoadenosine phosphosulfate reductasePRO_0000100662Add
BLAST

Proteomic databases

MaxQBiP18408.
PaxDbiP18408.
PeptideAtlasiP18408.

Expressioni

Gene expression databases

GenevestigatoriP18408.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TRX1P222171EBI-11481,EBI-19607

Protein-protein interaction databases

BioGridi36340. 49 interactions.
DIPiDIP-6611N.
MINTiMINT-677033.
STRINGi4932.YPR167C.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Helixi7 – 93
Beta strandi11 – 133
Helixi14 – 2411
Helixi30 – 4011
Beta strandi42 – 476
Helixi52 – 6413
Turni65 – 673
Beta strandi72 – 765
Helixi83 – 9614
Helixi98 – 1003
Beta strandi105 – 1073
Helixi115 – 1228
Helixi126 – 1294
Helixi131 – 1388
Helixi140 – 14910
Beta strandi153 – 1564
Helixi161 – 1633
Helixi165 – 1695
Beta strandi172 – 1765
Turni177 – 1804
Beta strandi181 – 1844
Turni186 – 1894
Helixi192 – 20211
Helixi208 – 2125
Helixi220 – 2223
Turni232 – 2365
Turni246 – 2483
Helixi251 – 2533

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OQ2X-ray2.10A/B/C/D1-261[»]
ProteinModelPortaliP18408.
SMRiP18408. Positions 1-257.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18408.

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysH subfamily.Curated

Phylogenomic databases

eggNOGiCOG0175.
HOGENOMiHOG000249397.
InParanoidiP18408.
KOiK00390.
OMAiIGDVHST.
OrthoDBiEOG7P2Z3B.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.

Sequencei

Sequence statusi: Complete.

P18408-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTYHLNNDI IVTQEQLDHW NEQLIKLETP QEIIAWSIVT FPHLFQTTAF
60 70 80 90 100
GLTGLVTIDM LSKLSEKYYM PELLFIDTLH HFPQTLTLKN EIEKKYYQPK
110 120 130 140 150
NQTIHVYKPD GCESEADFAS KYGDFLWEKD DDKYDYLAKV EPAHRAYKEL
160 170 180 190 200
HISAVFTGRR KSQGSARSQL SIIEIDELNG ILKINPLINW TFEQVKQYID
210 220 230 240 250
ANNVPYNELL DLGYRSIGDY HSTQPVKEGE DERAGRWKGK AKTECGIHEA
260
SRFAQFLKQD A
Length:261
Mass (Da):30,380
Last modified:November 1, 1997 - v2
Checksum:i202AF2C20829513C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti242 – 26120KTECG…LKQDA → RPSVEFMKPADSRNF in AAA34774. (PubMed:2203779)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05591 Genomic DNA. Translation: AAA34774.1.
U25840 Genomic DNA. Translation: AAB68154.1.
AY693236 Genomic DNA. Translation: AAT93255.1.
BK006949 Genomic DNA. Translation: DAA11584.1.
PIRiS59826.
RefSeqiNP_015493.1. NM_001184264.1.

Genome annotation databases

EnsemblFungiiYPR167C; YPR167C; YPR167C.
GeneIDi856296.
KEGGisce:YPR167C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05591 Genomic DNA. Translation: AAA34774.1 .
U25840 Genomic DNA. Translation: AAB68154.1 .
AY693236 Genomic DNA. Translation: AAT93255.1 .
BK006949 Genomic DNA. Translation: DAA11584.1 .
PIRi S59826.
RefSeqi NP_015493.1. NM_001184264.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OQ2 X-ray 2.10 A/B/C/D 1-261 [» ]
ProteinModelPortali P18408.
SMRi P18408. Positions 1-257.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36340. 49 interactions.
DIPi DIP-6611N.
MINTi MINT-677033.
STRINGi 4932.YPR167C.

Proteomic databases

MaxQBi P18408.
PaxDbi P18408.
PeptideAtlasi P18408.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR167C ; YPR167C ; YPR167C .
GeneIDi 856296.
KEGGi sce:YPR167C.

Organism-specific databases

CYGDi YPR167c.
SGDi S000006371. MET16.

Phylogenomic databases

eggNOGi COG0175.
HOGENOMi HOG000249397.
InParanoidi P18408.
KOi K00390.
OMAi IGDVHST.
OrthoDBi EOG7P2Z3B.

Enzyme and pathway databases

UniPathwayi UPA00140 ; UER00206 .
BioCyci MetaCyc:MONOMER-384.
YEAST:YPR167C-MONOMER.

Miscellaneous databases

EvolutionaryTracei P18408.
NextBioi 981647.
PROi P18408.

Gene expression databases

Genevestigatori P18408.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
InterProi IPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF01507. PAPS_reduct. 1 hit.
[Graphical view ]
PIRSFi PIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsi TIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene-enzyme relationship in the sulfate assimilation pathway of Saccharomyces cerevisiae. Study of the 3'-phosphoadenylylsulfate reductase structural gene."
    Thomas D., Barbey R., Surdin-Kerjan Y.
    J. Biol. Chem. 265:15518-15524(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMET16_YEAST
AccessioniPrimary (citable) accession number: P18408
Secondary accession number(s): D6W4G8, Q06212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3