P18406 (CYR61_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein CYR61

Alternative name(s):
3CH61
CCN family member 1
Cysteine-rich angiogenic inducer 61
Insulin-like growth factor-binding protein 10
Short name=IBP-10
Short name=IGF-binding protein 10
Short name=IGFBP-10

Gene names

Name:	Cyr61
Synonyms:	Ccn1, Igfbp10

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 By similarity. CYR61-mediated gene regulation is dependent on heparin-binding By similarity. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 By similarity. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 By similarity.
Subunit structure	Interaction with integrins is heparin- and cell-type-dependent and promotes cell adhesion By similarity.
Subcellular location	Secreted.
Tissue specificity	Low in kidney, adrenal gland, testes, brain, and ovary, moderate in heart, uterus, and skeletal muscle, highest in lung.
Developmental stage	Expressed from G0/G1 through mid-G1 in normal cells, and at a constant level in rapidly growing cells.
Induction	By growth factors.
Sequence similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Biological process	Cell adhesion Chemotaxis
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding Heparin-binding
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process involved in heart morphogenesis Inferred from mutant phenotype PubMed 17023674. Source: MGI atrial septum morphogenesis Inferred from mutant phenotype PubMed 17023674. Source: MGI atrioventricular valve morphogenesis Inferred from mutant phenotype PubMed 17023674. Source: MGI chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW chondroblast differentiation Inferred from direct assay PubMed 9441684. Source: MGI chorio-allantoic fusion Inferred from mutant phenotype PubMed 12446788. Source: MGI extracellular matrix organization Inferred from direct assay PubMed 18757743. Source: MGI intussusceptive angiogenesis Inferred from mutant phenotype PubMed 12446788. Source: MGI labyrinthine layer blood vessel development Inferred from mutant phenotype PubMed 12446788. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype PubMed 17023674. Source: MGI osteoblast differentiation Inferred from genetic interaction PubMed 16581771. Source: MGI positive regulation of BMP signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from genetic interaction PubMed 19364818. Source: MGI positive regulation of cartilage development Inferred from mutant phenotype PubMed 9441684. Source: MGI positive regulation of cell differentiation Inferred from direct assay PubMed 9441684. Source: MGI positive regulation of cell migration Inferred from direct assay PubMed 16581771. Source: MGI positive regulation of cell-substrate adhesion Inferred from direct assay PubMed 18757743. Source: MGI positive regulation of ceramide biosynthetic process Inferred from direct assay PubMed 19364818. Source: MGI positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from genetic interaction PubMed 19364818. Source: MGI positive regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara positive regulation of osteoblast proliferation Inferred from electronic annotation. Source: Compara positive regulation of phospholipase activity Inferred from direct assay PubMed 19364818. Source: MGI positive regulation of protein kinase activity Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara reactive oxygen species metabolic process Inferred from direct assay PubMed 19364818. Source: MGI regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara regulation of cell growth Inferred from electronic annotation. Source: InterPro ventricular septum development Inferred from mutant phenotype PubMed 17023674. Source: MGI wound healing, spreading of cells Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	extracellular matrix binding Inferred from direct assay PubMed 18757743. Source: MGI heparin binding Inferred from electronic annotation. Source: UniProtKB-KW integrin binding Inferred from direct assay PubMed 12826661. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 379	355	Protein CYR61		PRO_0000014399
Regions
	Domain	25 – 94	70	IGFBP N-terminal
	Domain	98 – 164	67	VWFC
	Domain	226 – 271	46	TSP type-1
	Domain	284 – 358	75	CTCK
	Region	277 – 313	37	Heparin-binding
Amino acid modifications
	Disulfide bond	284 ↔ 321		By similarity
	Disulfide bond	301 ↔ 335		By similarity
	Disulfide bond	312 ↔ 351		By similarity
	Disulfide bond	315 ↔ 353		By similarity
	Disulfide bond	320 ↔ 357		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18406 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: FA6B5014B56A8EE9	FASTA	379	41,709
	10 20 30 40 50 60 MSSSTFRTLA VAVTLLHLTR LALSTCPAAC HCPLEAPKCA PGVGLVRDGC GCCKVCAKQL 70 80 90 100 110 120 NEDCSKTQPC DHTKGLECNF GASSTALKGI CRAQSEGRPC EYNSRIYQNG ESFQPNCKHQ 130 140 150 160 170 180 CTCIDGAVGC IPLCPQELSL PNLGCPNPRL VKVSGQCCEE WVCDEDSIKD SLDDQDDLLG 190 200 210 220 230 240 LDASEVELTR NNELIAIGKG SSLKRLPVFG TEPRVLFNPL HAHGQKCIVQ TTSWSQCSKS 250 260 270 280 290 300 CGTGISTRVT NDNPECRLVK ETRICEVRPC GQPVYSSLKK GKKCSKTKKS PEPVRFTYAG 310 320 330 340 350 360 CSSVKKYRPK YCGSCVDGRC CTPLQTRTVK MRFRCEDGEM FSKNVMMIQS CKCNYNCPHP 370 NEASFRLYSL FNDIHKFRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of cyr61, a growth factor-inducible immediate-early gene." O'Brien T.P., Yang G.P., Sanders L., Lau L.F. Mol. Cell. Biol. 10:3569-3577(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Fibroblast.
[2]	"Promoter function and structure of the growth factor-inducible immediate early gene cyr61." Latinkic B.V., O'Brien T.P., Lau L.F. Nucleic Acids Res. 19:3261-3267(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A/J. Tissue: Embryonic fibroblast.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Adhesion of human skin fibroblasts to Cyr61 is mediated through integrin alpha 6beta 1 and cell surface heparan sulfate proteoglycans." Chen N., Chen C.-C., Lau L.F. J. Biol. Chem. 275:24953-24961(2000) [PubMed] [Europe PMC] [Abstract] Cited for: HEPARIN-BINDING DOMAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32490 mRNA. Translation: AAA37512.1. X56790 Genomic DNA. Translation: CAA40109.1. BC066019 mRNA. Translation: AAH66019.1.
IPI	IPI00113117.
PIR	A35669.
RefSeq	NP_034646.1. NM_010516.2.
UniGene	Mm.1231.
3D structure databases
ProteinModelPortal	P18406.
SMR	P18406. Positions 228-270.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000029846.
PTM databases
PhosphoSite	P18406.
Proteomic databases
PaxDb	P18406.
PRIDE	P18406.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029846; ENSMUSP00000029846; ENSMUSG00000028195.
GeneID	16007.
KEGG	mmu:16007.
UCSC	uc008rqq.2. mouse.
Organism-specific databases
CTD	3491.
MGI	MGI:88613. Cyr61.
Phylogenomic databases
eggNOG	NOG82014.
HOGENOM	HOG000231462.
HOVERGEN	HBG000635.
InParanoid	P18406.
KO	K06829.
OMA	KCIVQTT.
OrthoDB	EOG4J3WH3.
Gene expression databases
ArrayExpress	P18406.
Bgee	P18406.
CleanEx	MM_CYR61.
Genevestigator	P18406.
GermOnline	ENSMUSG00000028195. Mus musculus.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR006207. Cys_knot_C. IPR000867. IGFBP-like. IPR012395. IGFBP_CNN. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000884. Thrombospondin_1_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00007. Cys_knot. 1 hit. PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. PF00093. VWC. 1 hit. [Graphical view]
PIRSF	PIRSF036495. IGFBP_rP_CNN. 1 hit.
SMART	SM00041. CT. 1 hit. SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
SUPFAM	SSF82895. TSP1. 1 hit.
PROSITE	PS01185. CTCK_1. 1 hit. PS01225. CTCK_2. 1 hit. PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	288810.
SOURCE	Search...

Entry information

Entry name

CYR61_MOUSE

Accession

Primary (citable) accession number: P18406

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 29, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program