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P18406 (CYR61_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein CYR61
Alternative name(s):
3CH61
CCN family member 1
Cysteine-rich angiogenic inducer 61
Insulin-like growth factor-binding protein 10
Short name=IBP-10
Short name=IGF-binding protein 10
Short name=IGFBP-10
Gene names
Name:Cyr61
Synonyms:Ccn1, Igfbp10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 By similarity. CYR61-mediated gene regulation is dependent on heparin-binding By similarity. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 By similarity. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 By similarity.

Subunit structure

Interaction with integrins is heparin- and cell-type-dependent and promotes cell adhesion By similarity.

Subcellular location

Secreted.

Tissue specificity

Low in kidney, adrenal gland, testes, brain, and ovary, moderate in heart, uterus, and skeletal muscle, highest in lung.

Developmental stage

Expressed from G0/G1 through mid-G1 in normal cells, and at a constant level in rapidly growing cells.

Induction

By growth factors.

Sequence similarities

Belongs to the CCN family.

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Contains 1 IGFBP N-terminal domain.

Contains 1 TSP type-1 domain.

Contains 1 VWFC domain.

Ontologies

Keywords
   Biological processCell adhesion
Chemotaxis
   Cellular componentSecreted
   DomainSignal
   LigandGrowth factor binding
Heparin-binding
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process involved in heart morphogenesis

Inferred from mutant phenotype PubMed 17023674. Source: MGI

atrial septum morphogenesis

Inferred from mutant phenotype PubMed 17023674. Source: MGI

atrioventricular valve morphogenesis

Inferred from mutant phenotype PubMed 17023674. Source: MGI

cell adhesion

Inferred from direct assay PubMed 12826661. Source: MGI

cell-cell adhesion

Inferred from direct assay PubMed 9441684. Source: MGI

chemotaxis

Inferred from electronic annotation. Source: UniProtKB-KW

chondroblast differentiation

Inferred from direct assay PubMed 9441684. Source: MGI

chorio-allantoic fusion

Inferred from mutant phenotype PubMed 12446788. Source: MGI

extracellular matrix organization

Inferred from direct assay PubMed 18757743. Source: MGI

intussusceptive angiogenesis

Inferred from mutant phenotype PubMed 12446788. Source: MGI

labyrinthine layer blood vessel development

Inferred from mutant phenotype PubMed 12446788. Source: MGI

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 17023674. Source: MGI

osteoblast differentiation

Inferred from genetic interaction PubMed 16581771. Source: MGI

positive regulation of BMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic process

Inferred from genetic interaction PubMed 19364818. Source: MGI

positive regulation of cartilage development

Inferred from mutant phenotype PubMed 9441684. Source: MGI

positive regulation of cell differentiation

Inferred from direct assay PubMed 9441684. Source: MGI

positive regulation of cell migration

Inferred from direct assay PubMed 16581771. Source: MGI

positive regulation of cell-substrate adhesion

Inferred from direct assay PubMed 18757743. Source: MGI

positive regulation of ceramide biosynthetic process

Inferred from direct assay PubMed 19364818. Source: MGI

positive regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from genetic interaction PubMed 19364818. Source: MGI

positive regulation of osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of osteoblast proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of phospholipase activity

Inferred from direct assay PubMed 19364818. Source: MGI

positive regulation of protein kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

reactive oxygen species metabolic process

Inferred from direct assay PubMed 19364818. Source: MGI

regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

regulation of cell growth

Inferred from electronic annotation. Source: InterPro

ventricular septum development

Inferred from mutant phenotype PubMed 17023674. Source: MGI

wound healing, spreading of cells

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionextracellular matrix binding

Inferred from direct assay PubMed 18757743. Source: MGI

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

integrin binding

Inferred from direct assay PubMed 12826661. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 379355Protein CYR61
PRO_0000014399

Regions

Domain25 – 9470IGFBP N-terminal
Domain98 – 16467VWFC
Domain226 – 27146TSP type-1
Domain284 – 35875CTCK
Region277 – 31337Heparin-binding

Amino acid modifications

Disulfide bond284 ↔ 321 By similarity
Disulfide bond301 ↔ 335 By similarity
Disulfide bond312 ↔ 351 By similarity
Disulfide bond315 ↔ 353 By similarity
Disulfide bond320 ↔ 357 By similarity

Sequences

Sequence LengthMass (Da)Tools
P18406 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: FA6B5014B56A8EE9

FASTA37941,709
        10         20         30         40         50         60 
MSSSTFRTLA VAVTLLHLTR LALSTCPAAC HCPLEAPKCA PGVGLVRDGC GCCKVCAKQL 

        70         80         90        100        110        120 
NEDCSKTQPC DHTKGLECNF GASSTALKGI CRAQSEGRPC EYNSRIYQNG ESFQPNCKHQ 

       130        140        150        160        170        180 
CTCIDGAVGC IPLCPQELSL PNLGCPNPRL VKVSGQCCEE WVCDEDSIKD SLDDQDDLLG 

       190        200        210        220        230        240 
LDASEVELTR NNELIAIGKG SSLKRLPVFG TEPRVLFNPL HAHGQKCIVQ TTSWSQCSKS 

       250        260        270        280        290        300 
CGTGISTRVT NDNPECRLVK ETRICEVRPC GQPVYSSLKK GKKCSKTKKS PEPVRFTYAG 

       310        320        330        340        350        360 
CSSVKKYRPK YCGSCVDGRC CTPLQTRTVK MRFRCEDGEM FSKNVMMIQS CKCNYNCPHP 

       370 
NEASFRLYSL FNDIHKFRD 

« Hide

References

« Hide 'large scale' references
[1]"Expression of cyr61, a growth factor-inducible immediate-early gene."
O'Brien T.P., Yang G.P., Sanders L., Lau L.F.
Mol. Cell. Biol. 10:3569-3577(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Fibroblast.
[2]"Promoter function and structure of the growth factor-inducible immediate early gene cyr61."
Latinkic B.V., O'Brien T.P., Lau L.F.
Nucleic Acids Res. 19:3261-3267(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A/J.
Tissue: Embryonic fibroblast.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Adhesion of human skin fibroblasts to Cyr61 is mediated through integrin alpha 6beta 1 and cell surface heparan sulfate proteoglycans."
Chen N., Chen C.-C., Lau L.F.
J. Biol. Chem. 275:24953-24961(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: HEPARIN-BINDING DOMAIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M32490 mRNA. Translation: AAA37512.1.
X56790 Genomic DNA. Translation: CAA40109.1.
BC066019 mRNA. Translation: AAH66019.1.
PIRA35669.
RefSeqNP_034646.1. NM_010516.2.
UniGeneMm.1231.

3D structure databases

ProteinModelPortalP18406.
SMRP18406. Positions 25-167, 230-271, 293-355.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000029846.

PTM databases

PhosphoSiteP18406.

Proteomic databases

PaxDbP18406.
PRIDEP18406.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029846; ENSMUSP00000029846; ENSMUSG00000028195.
GeneID16007.
KEGGmmu:16007.
UCSCuc008rqq.2. mouse.

Organism-specific databases

CTD3491.
MGIMGI:88613. Cyr61.

Phylogenomic databases

eggNOGNOG82014.
HOGENOMHOG000231462.
HOVERGENHBG000635.
InParanoidP18406.
KOK06829.
OMAKCIVQTT.
OrthoDBEOG7N8ZXB.
PhylomeDBP18406.
TreeFamTF326070.

Gene expression databases

ArrayExpressP18406.
BgeeP18406.
CleanExMM_CYR61.
GenevestigatorP18406.

Family and domain databases

InterProIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288810.
PROP18406.
SOURCESearch...

Entry information

Entry nameCYR61_MOUSE
AccessionPrimary (citable) accession number: P18406
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot