Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein CYR61

Gene

Cyr61

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CYR61-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).By similarity

GO - Molecular functioni

  • extracellular matrix binding Source: MGI
  • heparin binding Source: GO_Central
  • integrin binding Source: MGI

GO - Biological processi

  • apoptotic process involved in heart morphogenesis Source: MGI
  • atrial septum morphogenesis Source: MGI
  • atrioventricular valve morphogenesis Source: MGI
  • cell adhesion Source: MGI
  • cell-cell signaling Source: GO_Central
  • chemotaxis Source: UniProtKB-KW
  • chondroblast differentiation Source: MGI
  • chorio-allantoic fusion Source: MGI
  • extracellular matrix organization Source: MGI
  • intussusceptive angiogenesis Source: MGI
  • labyrinthine layer blood vessel development Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • osteoblast differentiation Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of BMP signaling pathway Source: MGI
  • positive regulation of cartilage development Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of cell migration Source: MGI
  • positive regulation of cell-substrate adhesion Source: MGI
  • positive regulation of ceramide biosynthetic process Source: MGI
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • positive regulation of osteoblast differentiation Source: MGI
  • positive regulation of osteoblast proliferation Source: MGI
  • positive regulation of phospholipase activity Source: MGI
  • positive regulation of protein kinase activity Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • reactive oxygen species metabolic process Source: MGI
  • regulation of cell growth Source: InterPro
  • regulation of ERK1 and ERK2 cascade Source: MGI
  • signal transduction Source: GO_Central
  • single organismal cell-cell adhesion Source: MGI
  • ventricular septum development Source: MGI
  • wound healing, spreading of cells Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Chemotaxis

Keywords - Ligandi

Growth factor binding, Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CYR61
Alternative name(s):
3CH61
CCN family member 1
Cysteine-rich angiogenic inducer 61
Insulin-like growth factor-binding protein 10
Short name:
IBP-10
Short name:
IGF-binding protein 10
Short name:
IGFBP-10
Gene namesi
Name:Cyr61
Synonyms:Ccn1, Igfbp10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:88613. Cyr61.

Subcellular locationi

GO - Cellular componenti

  • extracellular matrix Source: UniProtKB
  • proteinaceous extracellular matrix Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 379355Protein CYR61PRO_0000014399Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi284 ↔ 321By similarity
Disulfide bondi301 ↔ 335By similarity
Disulfide bondi312 ↔ 351By similarity
Disulfide bondi315 ↔ 353By similarity
Disulfide bondi320 ↔ 357By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP18406.
PaxDbiP18406.
PRIDEiP18406.

PTM databases

PhosphoSiteiP18406.

Expressioni

Tissue specificityi

Low in kidney, adrenal gland, testes, brain, and ovary, moderate in heart, uterus, and skeletal muscle, highest in lung.

Developmental stagei

Expressed from G0/G1 through mid-G1 in normal cells, and at a constant level in rapidly growing cells.

Inductioni

By growth factors.

Gene expression databases

BgeeiP18406.
CleanExiMM_CYR61.
ExpressionAtlasiP18406. baseline and differential.
GenevisibleiP18406. MM.

Interactioni

Subunit structurei

Interaction with integrins is heparin- and cell-type-dependent and promotes cell adhesion.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029846.

Structurei

3D structure databases

ProteinModelPortaliP18406.
SMRiP18406. Positions 294-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 9470IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini98 – 16467VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini226 – 27146TSP type-1PROSITE-ProRule annotationAdd
BLAST
Domaini284 – 35875CTCKPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni277 – 31337Heparin-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the CCN family.Curated
Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG82014.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiP18406.
KOiK06829.
OMAiKCIVQTT.
OrthoDBiEOG7N8ZXB.
PhylomeDBiP18406.
TreeFamiTF326070.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSTFRTLA VAVTLLHLTR LALSTCPAAC HCPLEAPKCA PGVGLVRDGC
60 70 80 90 100
GCCKVCAKQL NEDCSKTQPC DHTKGLECNF GASSTALKGI CRAQSEGRPC
110 120 130 140 150
EYNSRIYQNG ESFQPNCKHQ CTCIDGAVGC IPLCPQELSL PNLGCPNPRL
160 170 180 190 200
VKVSGQCCEE WVCDEDSIKD SLDDQDDLLG LDASEVELTR NNELIAIGKG
210 220 230 240 250
SSLKRLPVFG TEPRVLFNPL HAHGQKCIVQ TTSWSQCSKS CGTGISTRVT
260 270 280 290 300
NDNPECRLVK ETRICEVRPC GQPVYSSLKK GKKCSKTKKS PEPVRFTYAG
310 320 330 340 350
CSSVKKYRPK YCGSCVDGRC CTPLQTRTVK MRFRCEDGEM FSKNVMMIQS
360 370
CKCNYNCPHP NEASFRLYSL FNDIHKFRD
Length:379
Mass (Da):41,709
Last modified:November 1, 1990 - v1
Checksum:iFA6B5014B56A8EE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32490 mRNA. Translation: AAA37512.1.
X56790 Genomic DNA. Translation: CAA40109.1.
BC066019 mRNA. Translation: AAH66019.1.
CCDSiCCDS17895.1.
PIRiA35669.
RefSeqiNP_034646.1. NM_010516.2.
UniGeneiMm.1231.

Genome annotation databases

EnsembliENSMUST00000029846; ENSMUSP00000029846; ENSMUSG00000028195.
GeneIDi16007.
KEGGimmu:16007.
UCSCiuc008rqq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32490 mRNA. Translation: AAA37512.1.
X56790 Genomic DNA. Translation: CAA40109.1.
BC066019 mRNA. Translation: AAH66019.1.
CCDSiCCDS17895.1.
PIRiA35669.
RefSeqiNP_034646.1. NM_010516.2.
UniGeneiMm.1231.

3D structure databases

ProteinModelPortaliP18406.
SMRiP18406. Positions 294-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029846.

PTM databases

PhosphoSiteiP18406.

Proteomic databases

MaxQBiP18406.
PaxDbiP18406.
PRIDEiP18406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029846; ENSMUSP00000029846; ENSMUSG00000028195.
GeneIDi16007.
KEGGimmu:16007.
UCSCiuc008rqq.2. mouse.

Organism-specific databases

CTDi3491.
MGIiMGI:88613. Cyr61.

Phylogenomic databases

eggNOGiNOG82014.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiP18406.
KOiK06829.
OMAiKCIVQTT.
OrthoDBiEOG7N8ZXB.
PhylomeDBiP18406.
TreeFamiTF326070.

Miscellaneous databases

NextBioi288810.
PROiP18406.
SOURCEiSearch...

Gene expression databases

BgeeiP18406.
CleanExiMM_CYR61.
ExpressionAtlasiP18406. baseline and differential.
GenevisibleiP18406. MM.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of cyr61, a growth factor-inducible immediate-early gene."
    O'Brien T.P., Yang G.P., Sanders L., Lau L.F.
    Mol. Cell. Biol. 10:3569-3577(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Fibroblast.
  2. "Promoter function and structure of the growth factor-inducible immediate early gene cyr61."
    Latinkic B.V., O'Brien T.P., Lau L.F.
    Nucleic Acids Res. 19:3261-3267(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A/J.
    Tissue: Embryonic fibroblast.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Adhesion of human skin fibroblasts to Cyr61 is mediated through integrin alpha 6beta 1 and cell surface heparan sulfate proteoglycans."
    Chen N., Chen C.-C., Lau L.F.
    J. Biol. Chem. 275:24953-24961(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: HEPARIN-BINDING DOMAIN.

Entry informationi

Entry nameiCYR61_MOUSE
AccessioniPrimary (citable) accession number: P18406
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 24, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.