Reviewed, UniProtKB/Swiss-Prot P18405 (S5A1_HUMAN)

Names and origin

Protein names

Recommended name:
    3-oxo-5-alpha-steroid 4-dehydrogenase 1
    EC=1.3.99.5
Alternative name(s):
    Steroid 5-alpha-reductase 1
      Short name=S5AR
    SR type 1

Gene names

Name:

SRD5A1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Catalytic activity	A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta(4)-steroid + reduced acceptor.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Tissue specificity	Liver and prostate (at a low level).
Induction	Its expression is regulated by androgens such as testosterone.
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
biophysicochemical properties	pH dependence: Optimally active at alkaline pHs.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sex determination Ref.2 Non-traceable author statement. Source: ProtInc
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Ref.2 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	3-oxo-5-alpha-steroid 4-dehydrogenase 1		PRO_0000213674
Regions
	Transmembrane	12 – 29	18	Potential
	Transmembrane	86 – 106	21	Potential
	Transmembrane	111 – 131	21	Potential
	Transmembrane	151 – 171	21	Potential
	Transmembrane	209 – 229	21	Potential
Experimental info
	Sequence conflict	29 – 76	48	Missing in AAF14869. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P18405-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: AEF9FD5521E43AC4	FASTA	259	29,459
	10 20 30 40 50 60 MATATGVAEE RLLAALAYLQ CAVGCAVFAR NRQTNSVYGR HALPSHRLRV PARAAWVVQE 70 80 90 100 110 120 LPSLALPLYQ YASESAPRLR SAPNCILLAM FLVHYGHRCL IYPFLMRGGK PMPLLACTMA 130 140 150 160 170 180 IMFCTCNGYL QSRYLSHCAV YADDWVTDPR FLIGFGLWLT GMLINIHSDH ILRNLRKPGD 190 200 210 220 230 240 TGYKIPRGGL FEYVTAANYF GEIMEWCGYA LASWSVQGAA FAFFTFCFLS GRAKEHHEWY 250 LRKFEEYPKF RKIIIPFLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases." Andersson S., Russell D.W. Proc. Natl. Acad. Sci. U.S.A. 87:3640-3644(1990) [PubMed: 2339109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization and chromosomal mapping of a human steroid 5 alpha-reductase gene and pseudogene and mapping of the mouse homologue." Jenkins E.P., Hsieh C.L., Milatovich A., Normington K., Berman D.M., Francke U., Russell D.W. Genomics 11:1102-1112(1991) [PubMed: 1686016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Yu W., Sarginson J., Gibbs R.A. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adrenal gland.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	NIEHS SNPs program Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye and Urinary bladder.
[8]	"New steroid 5alpha-reductase type I (SRD5A1) homologous sequences on human chromosomes 6 and 8." Eminovic I., Liovic M., Prezelj J., Kocijancic A., Rozman D., Komel R. Pflugers Arch. 442:R187-R189(2001) [PubMed: 11678334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98 AND 155-187.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Wikipedia

5-alpha reductase entry

Cross-references

Sequence databases
EMBLGenBankDDBJ	M32313 mRNA. Translation: AAA35490.1. M68886 , M68882, M68883, M68884, M68885 Genomic DNA. Translation: AAA60995.1. AF052126 mRNA. Translation: AAC28620.1. AF113128 mRNA. Translation: AAF14869.1. BT006834 mRNA. Translation: AAP35480.1. AY341029 Genomic DNA. Translation: AAP88935.1. BC007033 mRNA. Translation: AAH07033.1. BC008673 mRNA. Translation: AAH08673.1. AF073301 Genomic DNA. Translation: AAC26862.1. AF073302 Genomic DNA. Translation: AAC26863.1. AF073303 Genomic DNA. Translation: AAC26864.1. AF073304 Genomic DNA. Translation: AAC26865.1.
IPI	IPI00032233.
PIR	A55274.
RefSeq	NP_001038.1.
UniGene	Hs.552
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P18405.
Genome annotation databases
Ensembl	ENSG00000145545. Homo sapiens. [Contig view]
GeneID	6715.
KEGG	hsa:6715.
Organism-specific databases
GeneCards	GC05P006686.
H-InvDB	HIX0004734.
HGNC	HGNC:11284. SRD5A1.
MIM	184753. gene.
PharmGKB	PA36112.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P18405.
HOVERGEN	P18405.
OMA	P18405. SAANYFG.
Enzyme and pathway databases
BRENDA	1.3.99.5. 247.
Gene expression databases
ArrayExpress	P18405.
Bgee	P18405.
CleanEx	HS_SRD5A1.
GermOnline	ENSG00000145545. Homo sapiens.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB01126. Dutasteride. DB01216. Finasteride.
NextBio	26192.
SOURCE	Search...

Entry information

Entry name

S5A1_HUMAN

Accession

Primary (citable) accession number: P18405
Secondary accession number(s): Q9UHY4, Q9UP36, Q9UP37

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)