P18405 (S5A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 1
EC=1.3.1.22

Alternative name(s):
SR type 1
Steroid 5-alpha-reductase 1
Short name=S5AR 1

Gene names

Name:

SRD5A1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Catalytic activity	A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Tissue specificity	Liver and prostate (at a low level).
Induction	Its expression is regulated by androgens such as testosterone.
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
Biophysicochemical properties	pH dependence: Optimally active at alkaline pHs.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	androgen biosynthetic process Traceable author statement. Source: Reactome cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW sex determination Non-traceable author statement Ref.2. Source: ProtInc sex differentiation Inferred from electronic annotation. Source: UniProtKB-KW small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from direct assay PubMed 17986282. Source: UniProtKB cholestenone 5-alpha-reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	3-oxo-5-alpha-steroid 4-dehydrogenase 1		PRO_0000213674
Regions
	Transmembrane	12 – 29	18	Helical; Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Transmembrane	111 – 131	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	209 – 229	21	Helical; Potential
Experimental info
	Sequence conflict	29 – 76	48	Missing in AAF14869. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P18405 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: AEF9FD5521E43AC4	FASTA	259	29,459
	10 20 30 40 50 60 MATATGVAEE RLLAALAYLQ CAVGCAVFAR NRQTNSVYGR HALPSHRLRV PARAAWVVQE 70 80 90 100 110 120 LPSLALPLYQ YASESAPRLR SAPNCILLAM FLVHYGHRCL IYPFLMRGGK PMPLLACTMA 130 140 150 160 170 180 IMFCTCNGYL QSRYLSHCAV YADDWVTDPR FLIGFGLWLT GMLINIHSDH ILRNLRKPGD 190 200 210 220 230 240 TGYKIPRGGL FEYVTAANYF GEIMEWCGYA LASWSVQGAA FAFFTFCFLS GRAKEHHEWY 250 LRKFEEYPKF RKIIIPFLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases." Andersson S., Russell D.W. Proc. Natl. Acad. Sci. U.S.A. 87:3640-3644(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization and chromosomal mapping of a human steroid 5 alpha-reductase gene and pseudogene and mapping of the mouse homologue." Jenkins E.P., Hsieh C.L., Milatovich A., Normington K., Berman D.M., Francke U., Russell D.W. Genomics 11:1102-1112(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Yu W., Sarginson J., Gibbs R.A. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adrenal gland.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	NIEHS SNPs program Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye and Urinary bladder.
[10]	"New steroid 5alpha-reductase type I (SRD5A1) homologous sequences on human chromosomes 6 and 8." Eminovic I., Liovic M., Prezelj J., Kocijancic A., Rozman D., Komel R. Pflugers Arch. 442:R187-R189(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98 AND 155-187.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Wikipedia

5-alpha reductase entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32313 mRNA. Translation: AAA35490.1. M68886 , M68882, M68883, M68884, M68885 Genomic DNA. Translation: AAA60995.1. AF052126 mRNA. Translation: AAC28620.1. AF113128 mRNA. Translation: AAF14869.1. BT006834 mRNA. Translation: AAP35480.1. AY341029 Genomic DNA. Translation: AAP88935.1. AK313070 mRNA. Translation: BAG35898.1. CH471102 Genomic DNA. Translation: EAX08104.1. BC007033 mRNA. Translation: AAH07033.1. BC008673 mRNA. Translation: AAH08673.1. AF073301 Genomic DNA. Translation: AAC26862.1. AF073302 Genomic DNA. Translation: AAC26863.1. AF073303 Genomic DNA. Translation: AAC26864.1. AF073304 Genomic DNA. Translation: AAC26865.1.
IPI	IPI00032233.
PIR	A55274.
RefSeq	NP_001038.1. NM_001047.2.
UniGene	Hs.552.
3D structure databases
ProteinModelPortal	P18405.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000274192.
PTM databases
PhosphoSite	P18405.
Polymorphism databases
DMDM	134151.
Proteomic databases
PRIDE	P18405.
Protocols and materials databases
DNASU	6715.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274192; ENSP00000274192; ENSG00000145545.
GeneID	6715.
KEGG	hsa:6715.
UCSC	uc003jdw.3. human.
Organism-specific databases
CTD	6715.
GeneCards	GC05P006686.
HGNC	HGNC:11284. SRD5A1.
MIM	184753. gene.
neXtProt	NX_P18405.
PharmGKB	PA36112.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG82739.
HOGENOM	HOG000050133.
HOVERGEN	HBG003402.
InParanoid	P18405.
KO	K12343.
OMA	SAANYFG.
OrthoDB	EOG4VX25X.
PhylomeDB	P18405.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_15493. Steroid hormones.
Gene expression databases
ArrayExpress	P18405.
Bgee	P18405.
CleanEx	HS_SRD5A1.
Genevestigator	P18405.
GermOnline	ENSG00000145545. Homo sapiens.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P18405.
ChEMBL	CHEMBL1787.
DrugBank	DB01126. Dutasteride. DB01216. Finasteride.
GenomeRNAi	6715.
NextBio	26192.
SOURCE	Search...

Entry information

Entry name

S5A1_HUMAN

Accession

Primary (citable) accession number: P18405
Secondary accession number(s): B2R7Q1 , Q9UHY4, Q9UP36, Q9UP37

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.