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Protein

Sensor protein RstB

Gene

rstB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system RstB/RstA. RstB functions as a membrane-associated protein kinase that phosphorylates RstA (Probable).1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • peptidyl-histidine phosphorylation Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:RSTB-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein RstB (EC:2.7.13.3)
Gene namesi
Name:rstB
Synonyms:uspT
Ordered Locus Names:b1609, JW1601
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11233 rstB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 135PeriplasmicSequence analysisAdd BLAST111
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Topological domaini157 – 433CytoplasmicSequence analysisAdd BLAST277

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000748661 – 433Sensor protein RstBAdd BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP18392
PRIDEiP18392

Expressioni

Inductioni

Induced by low extracellular levels of magnesium via the PhoQ/PhoP two-component regulatory system.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261764, 18 interactors
DIPiDIP-10808N
IntActiP18392, 3 interactors
STRINGi316385.ECDH10B_1742

Structurei

3D structure databases

ProteinModelPortaliP18392
SMRiP18392
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini158 – 210HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini218 – 425Histidine kinasePROSITE-ProRule annotationAdd BLAST208

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105JJR Bacteria
ENOG4111IA6 LUCA
HOGENOMiHOG000258895
InParanoidiP18392
KOiK07639
OMAiDEYTFIQ
PhylomeDBiP18392

Family and domain databases

CDDicd06225 HAMP, 1 hit
cd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR003660 HAMP_dom
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00304 HAMP, 1 hit
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SUPFAMiSSF47384 SSF47384, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS50885 HAMP, 1 hit
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

P18392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLFIQFYL LLFVCFLVMS LLVGLVYKFT AERAGKQSLD DLMNSSLYLM
60 70 80 90 100
RSELREIPPH DWGKTLKEMD LNLSFDLRVE PLSKYHLDDI SMHRLRGGEI
110 120 130 140 150
VALDDQYTFL QRIPRSHYVL AVGPVPYLYY LHQMRLLDIA LIAFIAISLA
160 170 180 190 200
FPVFIWMRPH WQDMLKLEAA AQRFGDGHLN ERIHFDEGSS FERLGVAFNQ
210 220 230 240 250
MADNINALIA SKKQLIDGIA HELRTPLVRL RYRLEMSDNL SAAESQALNR
260 270 280 290 300
DISQLEALIE ELLTYARLDR PQNELHLSEP DLPLWLSTHL ADIQAVTPDK
310 320 330 340 350
TVRIKTLVQG HYAALDMRLM ERVLDNLLNN ALRYCHSTVE TSLLLSGNRA
360 370 380 390 400
TLIVEDDGPG IAPENREHIF EPFVRLDPSR DRSTGGCGLG LAIVHSIALA
410 420 430
MGGTVNCDTS ELGGARFSFS WPLWHNIPQF TSA
Length:433
Mass (Da):49,283
Last modified:October 1, 1996 - v2
Checksum:i62003BE4E48C7DDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41101 Genomic DNA Translation: AAA82082.1
X75466 Genomic DNA Translation: CAA53208.1
U00096 Genomic DNA Translation: AAC74681.1
AP009048 Genomic DNA Translation: BAA15347.1
PIRiC64917
RefSeqiNP_416126.1, NC_000913.3
WP_000732512.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74681; AAC74681; b1609
BAA15347; BAA15347; BAA15347
GeneIDi948870
KEGGiecj:JW1601
eco:b1609
PATRICifig|1411691.4.peg.653

Similar proteinsi

Entry informationi

Entry nameiRSTB_ECOLI
AccessioniPrimary (citable) accession number: P18392
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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