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Protein

Sensor protein RstB

Gene

rstB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system RstB/RstA. RstB functions as a membrane-associated protein kinase that phosphorylates RstA (Probable).1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • peptidyl-histidine phosphorylation Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:RSTB-MONOMER.
ECOL316407:JW1601-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein RstB (EC:2.7.13.3)
Gene namesi
Name:rstB
Synonyms:uspT
Ordered Locus Names:b1609, JW1601
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11233. rstB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence analysis
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Topological domaini25 – 135111PeriplasmicSequence analysisAdd
BLAST
Transmembranei136 – 15621HelicalSequence analysisAdd
BLAST
Topological domaini157 – 433277CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 433433Sensor protein RstBPRO_0000074866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei276 – 2761Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP18392.
PRIDEiP18392.

Expressioni

Inductioni

Induced by low extracellular levels of magnesium via the PhoQ/PhoP two-component regulatory system.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261764. 11 interactions.
DIPiDIP-10808N.
IntActiP18392. 3 interactions.
STRINGi511145.b1609.

Structurei

3D structure databases

ProteinModelPortaliP18392.
SMRiP18392. Positions 166-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini158 – 21053HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini218 – 425208Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105JJR. Bacteria.
ENOG4111IA6. LUCA.
HOGENOMiHOG000258895.
InParanoidiP18392.
KOiK07639.
OMAiQEGSHND.
OrthoDBiEOG6G4VQG.
PhylomeDBiP18392.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLFIQFYL LLFVCFLVMS LLVGLVYKFT AERAGKQSLD DLMNSSLYLM
60 70 80 90 100
RSELREIPPH DWGKTLKEMD LNLSFDLRVE PLSKYHLDDI SMHRLRGGEI
110 120 130 140 150
VALDDQYTFL QRIPRSHYVL AVGPVPYLYY LHQMRLLDIA LIAFIAISLA
160 170 180 190 200
FPVFIWMRPH WQDMLKLEAA AQRFGDGHLN ERIHFDEGSS FERLGVAFNQ
210 220 230 240 250
MADNINALIA SKKQLIDGIA HELRTPLVRL RYRLEMSDNL SAAESQALNR
260 270 280 290 300
DISQLEALIE ELLTYARLDR PQNELHLSEP DLPLWLSTHL ADIQAVTPDK
310 320 330 340 350
TVRIKTLVQG HYAALDMRLM ERVLDNLLNN ALRYCHSTVE TSLLLSGNRA
360 370 380 390 400
TLIVEDDGPG IAPENREHIF EPFVRLDPSR DRSTGGCGLG LAIVHSIALA
410 420 430
MGGTVNCDTS ELGGARFSFS WPLWHNIPQF TSA
Length:433
Mass (Da):49,283
Last modified:October 1, 1996 - v2
Checksum:i62003BE4E48C7DDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41101 Genomic DNA. Translation: AAA82082.1.
X75466 Genomic DNA. Translation: CAA53208.1.
U00096 Genomic DNA. Translation: AAC74681.1.
AP009048 Genomic DNA. Translation: BAA15347.1.
PIRiC64917.
RefSeqiNP_416126.1. NC_000913.3.
WP_000732512.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74681; AAC74681; b1609.
BAA15347; BAA15347; BAA15347.
GeneIDi948870.
KEGGiecj:JW1601.
eco:b1609.
PATRICi32118520. VBIEscCol129921_1680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41101 Genomic DNA. Translation: AAA82082.1.
X75466 Genomic DNA. Translation: CAA53208.1.
U00096 Genomic DNA. Translation: AAC74681.1.
AP009048 Genomic DNA. Translation: BAA15347.1.
PIRiC64917.
RefSeqiNP_416126.1. NC_000913.3.
WP_000732512.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP18392.
SMRiP18392. Positions 166-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261764. 11 interactions.
DIPiDIP-10808N.
IntActiP18392. 3 interactions.
STRINGi511145.b1609.

Proteomic databases

PaxDbiP18392.
PRIDEiP18392.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74681; AAC74681; b1609.
BAA15347; BAA15347; BAA15347.
GeneIDi948870.
KEGGiecj:JW1601.
eco:b1609.
PATRICi32118520. VBIEscCol129921_1680.

Organism-specific databases

EchoBASEiEB1215.
EcoGeneiEG11233. rstB.

Phylogenomic databases

eggNOGiENOG4105JJR. Bacteria.
ENOG4111IA6. LUCA.
HOGENOMiHOG000258895.
InParanoidiP18392.
KOiK07639.
OMAiQEGSHND.
OrthoDBiEOG6G4VQG.
PhylomeDBiP18392.

Enzyme and pathway databases

BioCyciEcoCyc:RSTB-MONOMER.
ECOL316407:JW1601-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP18392.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Kuempel P.L.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "tus, the trans-acting gene required for termination of DNA replication in Escherichia coli, encodes a DNA-binding protein."
    Hill T.M., Tecklenburg M.L., Pelletier A.J., Kuempel P.L.
    Proc. Natl. Acad. Sci. U.S.A. 86:1593-1597(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 251-433.
  6. "The tus gene of Escherichia coli: autoregulation, analysis of flanking sequences and identification of a complementary system in Salmonella typhimurium."
    Roecklein B., Pelletier A.J., Kuempel P.L.
    Res. Microbiol. 142:169-175(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO SENSORY TRANSDUCTION PROTEINS.
  7. "In vivo characterization of tus gene expression in Escherichia coli."
    Roecklein B.A., Kuempel P.L.
    Mol. Microbiol. 6:1655-1661(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MAPPING.
  8. "Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli."
    Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R.
    J. Bacteriol. 185:3696-3702(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12.
  9. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
    Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
    J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, AUTOPHOSPHORYLATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  10. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRSTB_ECOLI
AccessioniPrimary (citable) accession number: P18392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 1, 1996
Last modified: February 17, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.