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Protein

Endoglucanase

Gene
N/A
Organism
Cellulomonas uda
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei53 – 531Proton donorBy similarity
Active sitei110 – 1101NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
OrganismiCellulomonas uda
Taxonomic identifieri1714 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 359336EndoglucanasePRO_0000007933Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP18336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLRALVAVI VTTAVMLVPR AWAQTAWERY KARFMMPDAR IIDTANGNVS
60 70 80 90 100
HTEGQGFAML LAVANNDRPA FDKLWQWTDS TLRDKSNGLF YWRYNPVAPD
110 120 130 140 150
PIADKNNATD GDTLIAWALL RAQKQWQDKR YATASDAITA SLLKYTVVTF
160 170 180 190 200
AGRQVMLPGV KGFNRNDHLN LNPSYFIFPA WRAFAERTHL TAWRTLQSDG
210 220 230 240 250
QALLGQMGWG KSHLPSDWVA LRADGKMLPA KEWPPRMSFD AIRIPLYISW
260 270 280 290 300
VDPHSALLAP WKAWMQSYPR LQTPAWINVS TNEVAPWNMA GGLLAVRDLT
310 320 330 340 350
LGEPLERRRL TTRMIITPPA SSCWSGWRNR ISASAVMALQ VSQPVCLRAE

RKEQERLTM
Length:359
Mass (Da):40,690
Last modified:October 31, 1990 - v1
Checksum:i0445D7571B683148
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36503 Genomic DNA. Translation: AAA23090.1.
PIRiI40696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36503 Genomic DNA. Translation: AAA23090.1.
PIRiI40696.

3D structure databases

ProteinModelPortaliP18336.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of a cellulase gene of Cellulomonas uda CB4."
    Nakamura K., Misawa N., Kitamura K.
    J. Biotechnol. 4:247-254(1985)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-37.
    Strain: CB4.

Entry informationi

Entry nameiGUN_CELUD
AccessioniPrimary (citable) accession number: P18336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1990
Last sequence update: October 31, 1990
Last modified: September 30, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.