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P18330

- SYI1_PSEFL

UniProt

P18330 - SYI1_PSEFL

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Protein

Isoleucine--tRNA ligase 1

Gene

ileS1

Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity).By similarity
Confers resistance to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog produced by P.fluorescens NCIMB 10586 itself that competitively inhibits activation by Ile-tRNA synthetase, thus inhibiting protein biosynthesis.

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylateBy similarity
Binding sitei611 – 6111ATPBy similarity
Metal bindingi906 – 9061ZincBy similarity
Metal bindingi909 – 9091ZincBy similarity
Metal bindingi926 – 9261ZincBy similarity
Metal bindingi929 – 9291ZincBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Antibiotic resistance, Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

SABIO-RKP18330.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 1 (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase 1
Short name:
IleRS 1
Gene namesi
Name:ileS1
Synonyms:ileS
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligase 1PRO_0000098445Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP18330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi608 – 6125"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) (By similarity).By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18330-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWNSIGLYGK LREIGKDRPK
60 70 80 90 100
FVLHDGPPYA NGTIHIGHAL NKILKDMILR SKTLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVEVTY GKNLGADKTR ELCRAYATEQ IEGQKSEFIR LGVLGEWDNP
160 170 180 190 200
YKTMNFKNEA GEIRALAEIV KGGFVFKGLK PVNWCFDCGS ALAEAEVEYE
210 220 230 240 250
DKKSSTIDVA FPIADDAKLA EAFGLASLAK PAAIVIWTTT PWTIPANQAL
260 270 280 290 300
NVHPEFTYAL VDVGDRLLVL AEEMVESCLA RYELQGSVIA TATGSALELI
310 320 330 340 350
NFRHPFYDRL SPVYLADYVE LGSGTGIVHC SPAYGVDDFV ICKKYGMVND
360 370 380 390 400
DIINPVQSNG VYVPSLEFFG GQFIFKADQP IIEKLREVGA LMQTAAIQHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF IGMDKEPTSG DTLRVRSLKA IEDTKFVPSW
460 470 480 490 500
GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LNKESGELHP RTVELMEVVA
510 520 530 540 550
QRVEQQGIEA WFKLDAAELL GDEAPLYDKI SDTLDVWFDS GTTHWHVLRG
560 570 580 590 600
SHPMGHETGP RADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF
610 620 630 640 650
TVDETGRKMS KSLKNVIEPK KINDTLGADI MRLWVASTDY SGEIAVSDQI
660 670 680 690 700
LARSADAYRR IRNTARFMLS NLTGFNPASD LLPAEDMLAL DRWAVDRTLL
710 720 730 740 750
LQRELQEHYG EYRFWNVYSK IHNFCVQELG GFYLDIIKDR QYTTGANSKA
760 770 780 790 800
RRSAQTALYH ISEALVRWIA PILAFTADEL WEYLPGERNE SVMLNTWYEG
810 820 830 840 850
LTELPADFEL GREYWEGVMA VKVAVNKELE VQRAAKAVGG NLQAEVTLFA
860 870 880 890 900
EDGLTADLAK LSNELRFVLI TSTASLAPFT QAPADAVATE VPGLKLKVVK
910 920 930 940
SAFPKCARCW HCREDVGVNP EHPEICGRCV DNISGEGEVR HYA
Length:943
Mass (Da):105,719
Last modified:November 1, 1995 - v2
Checksum:i6827B2BF2EB89FC0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 292IL → SC(PubMed:2121716)Curated
Sequence conflicti755 – 7562QT → DR(PubMed:2121716)Curated
Sequence conflicti758 – 7592LY → VP(PubMed:2121716)Curated
Sequence conflicti761 – 7644ISEA → QRR(PubMed:2121716)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80132 Genomic DNA. Translation: CAA56431.1.
M35366 Genomic DNA. Translation: AAA25883.1.
M35367 Genomic DNA. Translation: AAA26022.1. Sequence problems.
PIRiA37152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80132 Genomic DNA. Translation: CAA56431.1 .
M35366 Genomic DNA. Translation: AAA25883.1 .
M35367 Genomic DNA. Translation: AAA26022.1 . Sequence problems.
PIRi A37152.

3D structure databases

ProteinModelPortali P18330.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P18330.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Relationship of protein structure of isoleucyl-tRNA synthetase with pseudomonic acid resistance of Escherichia coli. A proposed mode of action of pseudomonic acid as an inhibitor of isoleucyl-tRNA synthetase."
    Yanagisawa T., Lee J.T., Wu H.C., Kawakami M.
    J. Biol. Chem. 269:24304-24309(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49323 / NCIMB 10586.
  2. "Nucleotide sequence of the Pseudomonas fluorescens signal peptidase II gene (lsp) and flanking genes."
    Isaki L., Beers R., Wu H.C.
    J. Bacteriol. 172:6512-6517(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29 AND 701-943.
    Strain: ATCC 49323 / NCIMB 10586.
  3. "How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid? Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defense."
    Yanagisawa T., Kawakami M.
    J. Biol. Chem. 278:25887-25894(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: RESISTANCE TO MUPIROCIN.
    Strain: ATCC 49323 / NCIMB 10586.

Entry informationi

Entry nameiSYI1_PSEFL
AccessioniPrimary (citable) accession number: P18330
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

P.fluorescens NCIMB 10586 possesses two distinct IleRSs (IleRS-R1 and IleRS-R2), each with a different level of sensitivity to mupirocin. Purified IleRs-R2 shows no sensitivity to mupirocin even at a concentration of 5 mM, 100'000 fold higher than the Ki value of IleRS-R1.

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3