Reviewed, UniProtKB/Swiss-Prot P18319 (UREG_KLEAE)

Names and origin

Protein names

Recommended name:
    Urease accessory protein ureG

Gene names

Name:

ureG

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by ureG. HAMAP MF_01389
Enzyme regulation	Activation of apourease within the ureDFG-apoprotein complex is inhibited by zinc, copper and cobalt. HAMAP MF_01389
Subunit structure	Homodimer By similarity. UreD, ureF and ureG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of ureC. The ureE protein probably delivers the nickel.
Subcellular location	Cytoplasm. Ref.3
Sequence similarities	Belongs to the ureG family.
Mass spectrometry	Molecular mass is 21814±20 Da from positions 2 - 205. Determined by MALDI. Ref.3

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed HAMAP MF_01389
	Chain	2 – 205	204	Urease accessory protein ureG HAMAP MF_01389		PRO_0000067666
Regions
	Nucleotide binding	14 – 21	8	GTP Probable
Experimental info
	Mutagenesis	20	1	K → A: Does not produce active urease, no ureDFG-apourease complexes are formed. Ref.3
	Mutagenesis	21	1	T → A: Does not produce active urease, no ureDFG-apourease complexes are formed. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P18319-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 79BE91D1DEE90A81	FASTA	205	21,944
	10 20 30 40 50 60 MNSYKHPLRV GVGGPVGSGK TALLEALCKA MRDTWQLAVV TNDIYTKEDQ RILTEAGALA 70 80 90 100 110 120 PERIVGVETG GCPHTAIRED ASMNLAAVEA LSEKFGNLDL IFVESGGDNL SATFSPELAD 130 140 150 160 170 180 LTIYVIDVAE GEKIPRKGGP GITKSDFLVI NKTDLAPYVG ASLEVMASDT QRMRGDRPWT 190 200 FTNLKQGDGL STIIAFLEDK GMLGK

References

[1]	"Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation." Mulrooney S.B., Hausinger R.P. J. Bacteriol. 172:5837-5843(1990) [PubMed: 2211515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CG253.
[2]	"Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis." Lee M.H., Mulrooney S.B., Renner M.J., Markowicz Y., Hausinger R.P. J. Bacteriol. 174:4324-4330(1992) [PubMed: 1624427] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-5.
[3]	"Characterization of UreG, identification of a UreD-UreF-UreG complex, and evidence suggesting that a nucleotide-binding site in UreG is required for in vivo metallocenter assembly of Klebsiella aerogenes urease." Moncrief M.B.C., Hausinger R.P. J. Bacteriol. 179:4081-4086(1997) [PubMed: 9209019] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY, MUTAGENESIS OF LYS-20 AND THR-21.
[4]	"GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins." Soriano A., Hausinger R.P. Proc. Natl. Acad. Sci. U.S.A. 96:11140-11144(1999) [PubMed: 10500143] [Abstract] Cited for: COMPLEX FORMATION, GTP HYDROLYSIS, INTERACTION WITH UREA; UREB; UREC; URED AND UREF.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M36068 Genomic DNA. Translation: AAA25154.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_01389. [Tree]
InterPro	IPR003495. Cbl_biosynth_CobW-like. IPR012202. NiFe_hyd/urase_mat_GTPase. IPR004400. UreG. [Graphical view]
Pfam	PF02492. cobW. 1 hit. [Graphical view]
PIRSF	PIRSF005624. Ni-bind_GTPase. 1 hit.
TIGRFAMs	TIGR00101. ureG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREG_KLEAE

Accession

Primary (citable) accession number: P18319

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	September 22, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)