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Protein

Sapecin

Gene
N/A
Organism
Sarcophaga peregrina (Flesh fly) (Boettcherisca peregrina)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sapecins, which are potent bactericidal proteins, are produced in response to injury. Sapecin is cytotoxic to Gram-positive bacteria, and to a lesser extent against Gram-negative bacteria.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin

Keywords - Biological processi

Immunity, Innate immunity

Protein family/group databases

TCDBi1.C.47.1.3. the insect/fungal defensin (insect/fungal defensin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sapecin
OrganismiSarcophaga peregrina (Flesh fly) (Boettcherisca peregrina)
Taxonomic identifieri7386 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaOestroideaSarcophagidaeSarcophagaBoettcherisca

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Propeptidei24 – 54311 PublicationPRO_0000006756Add
BLAST
Peptidei55 – 9440SapecinPRO_0000006757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 84PROSITE-ProRule annotation1 Publication
Disulfide bondi70 ↔ 90PROSITE-ProRule annotation1 Publication
Disulfide bondi74 ↔ 92PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Expressioni

Tissue specificityi

Hemocytes and fat body.

Inductioni

By injury to the larval cell wall.

Structurei

Secondary structure

1
94
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi57 – 604Combined sources
Helixi68 – 7811Combined sources
Beta strandi80 – 856Combined sources
Turni86 – 883Combined sources
Beta strandi89 – 935Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L4VNMR-A55-94[»]
ProteinModelPortaliP18313.
SMRiP18313. Positions 55-94.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18313.

Family & Domainsi

Sequence similaritiesi

Belongs to the invertebrate defensin family. Type 1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR017982. Defensin_insect.
IPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF01097. Defensin_2. 1 hit.
[Graphical view]
PRINTSiPR00271. DEFENSIN.
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSFIVLAVT LCLAAFFMGQ SVASPAAAAE ESKFVDGLHA LKTIEPELHG
60 70 80 90
RYKRATCDLL SGTGINHSAC AAHCLLRGNR GGYCNGKAVC VCRN
Length:94
Mass (Da):9,915
Last modified:November 1, 1990 - v1
Checksum:i36170AB3073EC947
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04053 mRNA. Translation: AAA29984.1.
PIRiA31792.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04053 mRNA. Translation: AAA29984.1.
PIRiA31792.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L4VNMR-A55-94[»]
ProteinModelPortaliP18313.
SMRiP18313. Positions 55-94.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.47.1.3. the insect/fungal defensin (insect/fungal defensin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP18313.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR017982. Defensin_insect.
IPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF01097. Defensin_2. 1 hit.
[Graphical view]
PRINTSiPR00271. DEFENSIN.
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of cDNA for sapecin and unique expression of the sapecin gene during the development of Sarcophaga peregrina."
    Matsuyama K., Natori S.
    J. Biol. Chem. 263:17117-17121(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Purification of three antibacterial proteins from the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina."
    Matsuyama K., Natori S.
    J. Biol. Chem. 263:17112-17116(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 55-94.
  3. "Determination of the disulfide array in sapecin, an antibacterial peptide of Sarcophaga peregrina (flesh fly)."
    Kuzuhara T., Nakajima Y., Matsuyama K., Natori S.
    J. Biochem. 107:514-518(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  4. "1H nuclear magnetic resonance study of the solution conformation of an antibacterial protein, sapecin."
    Hanzawa H., Shimada I., Kuzuhara T., Komano H., Kohda D., Inagaki F., Natori S., Arata Y.
    FEBS Lett. 269:413-420(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiSAPE_SARPE
AccessioniPrimary (citable) accession number: P18313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 11, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.