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P18294

- KCRF_STRPU

UniProt

P18294 - KCRF_STRPU

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Protein

Creatine kinase, flagellar

Gene
N/A
Organism
Strongylocentrotus purpuratus (Purple sea urchin)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This axonemal protein participates in an energy shuttle that utilizes phosphocreatine to transfer the energy from ATP generated by the mitochondrion in the sperm head to dynein in the distal portions of the flagellum.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei232 – 2321ATP By similarity
Binding sitei277 – 2771ATP By similarity
Binding sitei605 – 6051ATP By similarity
Binding sitei706 – 7061ATP By similarity
Binding sitei749 – 7491ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1735ATP By similarity
Nucleotide bindingi333 – 3375ATP By similarity
Nucleotide bindingi542 – 5465ATP By similarity
Nucleotide bindingi734 – 7396ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. creatine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell projection organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase, flagellar (EC:2.7.3.2)
OrganismiStrongylocentrotus purpuratus (Purple sea urchin)
Taxonomic identifieri7668 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEchinodermataEleutherozoaEchinozoaEchinoideaEuechinoideaEchinaceaEchinoidaStrongylocentrotidaeStrongylocentrotus
ProteomesiUP000007110: Unplaced

Subcellular locationi

Cytoplasmcytoskeletonflagellum axoneme
Note: Associates specifically with the axoneme and may bind directly to polymerized microtubules.

GO - Cellular componenti

  1. cilium Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB-KW
  3. cytoskeleton Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11741174Creatine kinase, flagellarPRO_0000212010Add
BLAST

Proteomic databases

PRIDEiP18294.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi7668.P18294.

Structurei

3D structure databases

ProteinModelPortaliP18294.
SMRiP18294. Positions 61-419, 431-794, 807-1168.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 13987Phosphagen kinase N-terminal 1Add
BLAST
Repeati61 – 4143541; approximateAdd
BLAST
Domaini166 – 408243Phosphagen kinase C-terminal 1Add
BLAST
Domaini426 – 51287Phosphagen kinase N-terminal 2Add
BLAST
Repeati434 – 7873542; approximateAdd
BLAST
Domaini539 – 781243Phosphagen kinase C-terminal 2Add
BLAST
Domaini800 – 88687Phosphagen kinase N-terminal 3Add
BLAST
Repeati808 – 11613543; approximateAdd
BLAST
Domaini913 – 1155243Phosphagen kinase C-terminal 3Add
BLAST

Domaini

Contains three complete but non-identical creatine kinase segments flanked by unique regions.

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG3869.
InParanoidiP18294.
KOiK00933.

Family and domain databases

Gene3Di1.10.135.10. 3 hits.
3.30.590.10. 3 hits.
InterProiIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamiPF00217. ATP-gua_Ptrans. 3 hits.
PF02807. ATP-gua_PtransN. 3 hits.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 3 hits.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 3 hits.
PS51510. PHOSPHAGEN_KINASE_C. 3 hits.
PS51509. PHOSPHAGEN_KINASE_N. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18294-1 [UniParc]FASTAAdd to Basket

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MGCAASSQQT TATGGQPAAG EKANPAPANN NPNAANKAET TGAAEELTKE     50
SEPFVEPDPN YPDLSKHNNY LAESLTPSIY NKICNLRTLS GYSVDGCMQT 100
GVDNPGHPFI KTVGLVAGDE ECYDLFADLF DPTIDKRHNG YPRNAKHTTD 150
LNPDHLKGGD DFDPKYVLSC RVRTGRCIRG YGLPPHCTRA ERRDVEKVCK 200
DALATLDGPL KGTYYPLTGM TEEMQDKLIA DHFLFDKPVS PLLMSARMAR 250
DWPDGRGIWH NADKNFLVWI NEEDHTRVIS METSGNMKNV FKRFCNGLNK 300
VENALKAKGY EFSWNEHLGY VLTCPSNLGT GVRAGVHIKI PLFSKHAGFE 350
SILKHYRLQK RGTGGVDTAS TDGTFDISNL DRLGTSEVQQ VQSVVDGVKK 400
LIELEKALEK GSDISGQIPR DPAIVRAEQV KEGYPDLSKH NNHLAHCLTY 450
DIWKSLKDKK TPSGFTLDGC IQTGVMNPGH PHIMTVGMVA GDEESYDVFA 500
DIFDPVIDAR HGGYPKDAVH VTNINHADLK GGDNLDPKYV LSCRVRTGRS 550
IIGYSLPPHC TVEERAAVET ITIGALDKFD GDLQGKYYPL EGMSDETQTQ 600
LIDDHFLFDK PVSPLLTAAR MHRDWPQGRG IWHNENKNFL VWVNEEDHIR 650
VISMEKDGNM RAVFKRFCEG LQKFEQMIKK DGKEFMWNKH LGYVLTCPSN 700
LGTGLRAGVH VKLPLLSKYP RFDQILRALR LQKRGTGGVD TASTDGTFDI 750
SNLDRLGSSE VQQVQFVVDG VELLVQMEKK LEKGEDIFDI LPQQCRPKPP 800
IKPFSYDYPD FSLHNNWMSK CMTEEIYNKL CNLKTKGGVT LNDCIQTGID 850
NPGHPYIMTV GLVAGDEECY EVFAPLFDPV ISARHGGYAL DAKHPTNLNA 900
AELKGGDDLD PEFVLSCRVR TGRCIRGLAL PPCCTRAERA EVEKITTEAL 950
STLSGPLKGK YYPLTGMTDE EQEKLIEDHF LFDKPVSPLL LCANMARDWP 1000
QGRGIWHNDE KNFLVWVNEE DHTRVISMEK SGNMKRVFER FCDGLKKVED 1050
SIKSKGYQFM WNEHLGYVLT CPSNLGTGLR AGVHVKVPLL SQQKIFDSIL 1100
DHMRLQKRGT GGVDTASTDG TFDISNSDRI GFSEVHLVQQ LVDGVKLLVN 1150
LEKALMKGED INSLLPEKLR EDSS 1174
Length:1,174
Mass (Da):130,869
Last modified:November 1, 1990 - v1
Checksum:iB8F4FBA5AC48EE93
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33763 mRNA. Translation: AAA30049.1.
PIRiA43736.
RefSeqiNP_999687.1. NM_214522.1.
UniGeneiSpu.13.

Genome annotation databases

GeneIDi373287.
KEGGispu:373287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33763 mRNA. Translation: AAA30049.1 .
PIRi A43736.
RefSeqi NP_999687.1. NM_214522.1.
UniGenei Spu.13.

3D structure databases

ProteinModelPortali P18294.
SMRi P18294. Positions 61-419, 431-794, 807-1168.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7668.P18294.

Proteomic databases

PRIDEi P18294.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 373287.
KEGGi spu:373287.

Organism-specific databases

CTDi 373287.

Phylogenomic databases

eggNOGi COG3869.
InParanoidi P18294.
KOi K00933.

Family and domain databases

Gene3Di 1.10.135.10. 3 hits.
3.30.590.10. 3 hits.
InterProi IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view ]
Pfami PF00217. ATP-gua_Ptrans. 3 hits.
PF02807. ATP-gua_PtransN. 3 hits.
[Graphical view ]
SUPFAMi SSF48034. SSF48034. 3 hits.
PROSITEi PS00112. PHOSPHAGEN_KINASE. 3 hits.
PS51510. PHOSPHAGEN_KINASE_C. 3 hits.
PS51509. PHOSPHAGEN_KINASE_N. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication."
    Wothe D.D., Charbonneau H., Shapiro B.M.
    Proc. Natl. Acad. Sci. U.S.A. 87:5203-5207(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Testis.

Entry informationi

Entry nameiKCRF_STRPU
AccessioniPrimary (citable) accession number: P18294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: October 16, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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