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Reviewed, UniProtKB/Swiss-Prot P18294 (KCRF_STRPU)

Last modified September 22, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Creatine kinase, flagellar
    EC=2.7.3.2
OrganismStrongylocentrotus purpuratus (Purple sea urchin)
Taxonomic identifier7668 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Echinodermata › Eleutherozoa › Echinozoa › Echinoidea › Euechinoidea › Echinacea › Echinoida › Strongylocentrotidae › Strongylocentrotus

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Protein attributes

Sequence length1174 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This axonemal protein participates in an energy shuttle that utilizes phosphocreatine to transfer the energy from ATP generated by the mitochondrion in the sperm head to dynein in the distal portions of the flagellum.

Catalytic activity

ATP + creatine = ADP + phosphocreatine.

Subunit structure

Monomer.

Subcellular location

Cytoplasm › cytoskeleton › flagellum axoneme. Note: Associates specifically with the axoneme and may bind directly to polymerized microtubules.

Domain

Contains three complete but non-identical creatine kinase segments flanked by unique regions.

Sequence similarities

Belongs to the ATP:guanido phosphotransferase family.

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Ontologies

Keywords
   Biological processCilium biogenesis/degradation
   Cellular componentCell projection
Cilium
Cytoplasm
Cytoskeleton
Flagellum
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processcell projection organization

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcilium

Inferred from electronic annotation. Source: UniProtKB-KW

flagellar axoneme

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

creatine kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11741174Creatine kinase, flagellar
PRO_0000212010

Regions

Repeat61 – 4143541; approximate
Repeat434 – 7873542; approximate
Repeat808 – 11613543; approximate
Nucleotide binding169 – 1735ATP By similarity
Nucleotide binding333 – 3375ATP By similarity
Nucleotide binding542 – 5465ATP By similarity
Nucleotide binding734 – 7396ATP By similarity

Sites

Binding site2321ATP By similarity
Binding site2771ATP By similarity
Binding site6051ATP By similarity
Binding site7061ATP By similarity
Binding site7491ATP By similarity

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Sequences

Sequence LengthMass (Da)Tools
P18294-1 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: B8F4FBA5AC48EE93

FASTA1,174130,869
        10         20         30         40         50         60 
MGCAASSQQT TATGGQPAAG EKANPAPANN NPNAANKAET TGAAEELTKE SEPFVEPDPN 

        70         80         90        100        110        120 
YPDLSKHNNY LAESLTPSIY NKICNLRTLS GYSVDGCMQT GVDNPGHPFI KTVGLVAGDE 

       130        140        150        160        170        180 
ECYDLFADLF DPTIDKRHNG YPRNAKHTTD LNPDHLKGGD DFDPKYVLSC RVRTGRCIRG 

       190        200        210        220        230        240 
YGLPPHCTRA ERRDVEKVCK DALATLDGPL KGTYYPLTGM TEEMQDKLIA DHFLFDKPVS 

       250        260        270        280        290        300 
PLLMSARMAR DWPDGRGIWH NADKNFLVWI NEEDHTRVIS METSGNMKNV FKRFCNGLNK 

       310        320        330        340        350        360 
VENALKAKGY EFSWNEHLGY VLTCPSNLGT GVRAGVHIKI PLFSKHAGFE SILKHYRLQK 

       370        380        390        400        410        420 
RGTGGVDTAS TDGTFDISNL DRLGTSEVQQ VQSVVDGVKK LIELEKALEK GSDISGQIPR 

       430        440        450        460        470        480 
DPAIVRAEQV KEGYPDLSKH NNHLAHCLTY DIWKSLKDKK TPSGFTLDGC IQTGVMNPGH 

       490        500        510        520        530        540 
PHIMTVGMVA GDEESYDVFA DIFDPVIDAR HGGYPKDAVH VTNINHADLK GGDNLDPKYV 

       550        560        570        580        590        600 
LSCRVRTGRS IIGYSLPPHC TVEERAAVET ITIGALDKFD GDLQGKYYPL EGMSDETQTQ 

       610        620        630        640        650        660 
LIDDHFLFDK PVSPLLTAAR MHRDWPQGRG IWHNENKNFL VWVNEEDHIR VISMEKDGNM 

       670        680        690        700        710        720 
RAVFKRFCEG LQKFEQMIKK DGKEFMWNKH LGYVLTCPSN LGTGLRAGVH VKLPLLSKYP 

       730        740        750        760        770        780 
RFDQILRALR LQKRGTGGVD TASTDGTFDI SNLDRLGSSE VQQVQFVVDG VELLVQMEKK 

       790        800        810        820        830        840 
LEKGEDIFDI LPQQCRPKPP IKPFSYDYPD FSLHNNWMSK CMTEEIYNKL CNLKTKGGVT 

       850        860        870        880        890        900 
LNDCIQTGID NPGHPYIMTV GLVAGDEECY EVFAPLFDPV ISARHGGYAL DAKHPTNLNA 

       910        920        930        940        950        960 
AELKGGDDLD PEFVLSCRVR TGRCIRGLAL PPCCTRAERA EVEKITTEAL STLSGPLKGK 

       970        980        990       1000       1010       1020 
YYPLTGMTDE EQEKLIEDHF LFDKPVSPLL LCANMARDWP QGRGIWHNDE KNFLVWVNEE 

      1030       1040       1050       1060       1070       1080 
DHTRVISMEK SGNMKRVFER FCDGLKKVED SIKSKGYQFM WNEHLGYVLT CPSNLGTGLR 

      1090       1100       1110       1120       1130       1140 
AGVHVKVPLL SQQKIFDSIL DHMRLQKRGT GGVDTASTDG TFDISNSDRI GFSEVHLVQQ 

      1150       1160       1170 
LVDGVKLLVN LEKALMKGED INSLLPEKLR EDSS

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References

[1]"The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication."
Wothe D.D., Charbonneau H., Shapiro B.M.
Proc. Natl. Acad. Sci. U.S.A. 87:5203-5207(1990) [PubMed: 2367531] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Testis.

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Cross-references

Sequence databases

M33763 mRNA. Translation: AAA30049.1.
PIRA43736.
RefSeqNP_999687.1.
UniGeneSpu.13

3D structure databases

HSSPHSSP built from PDB template 2CRK based on UniProtKB P00563.
SMRP18294. Positions 431-794.
ModBaseSearch...

Genome annotation databases

GeneID373287.
KEGGspu:373287.

Organism-specific databases

CTD373287.

Enzyme and pathway databases

BRENDA2.7.3.2. 39474.

Family and domain databases

InterProIPR000749. ATP-guanido_PTrfase.
IPR014746. Gln_synth/guanido_kin_cat.
[Graphical view]
Gene3DG3DSA:1.10.135.10. ATP-gua_Ptrans. 3 hits.
G3DSA:3.30.590.10. ATP-gua_Ptrans. 3 hits.
PANTHERPTHR11547. ATP-gua_Ptrans. 1 hit.
PfamPF00217. ATP-gua_Ptrans. 3 hits.
PF02807. ATP-gua_PtransN. 3 hits.
[Graphical view]
PROSITEPS00112. GUANIDO_KINASE. 3 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameKCRF_STRPU
AccessionPrimary (citable) accession number: P18294
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: September 22, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents