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P18294

- KCRF_STRPU

UniProt

P18294 - KCRF_STRPU

Protein

Creatine kinase, flagellar

Gene
N/A
Organism
Strongylocentrotus purpuratus (Purple sea urchin)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    This axonemal protein participates in an energy shuttle that utilizes phosphocreatine to transfer the energy from ATP generated by the mitochondrion in the sperm head to dynein in the distal portions of the flagellum.

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei232 – 2321ATPPROSITE-ProRule annotation
    Binding sitei277 – 2771ATPPROSITE-ProRule annotation
    Binding sitei605 – 6051ATPPROSITE-ProRule annotation
    Binding sitei706 – 7061ATPPROSITE-ProRule annotation
    Binding sitei749 – 7491ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi169 – 1735ATPPROSITE-ProRule annotation
    Nucleotide bindingi333 – 3375ATPPROSITE-ProRule annotation
    Nucleotide bindingi542 – 5465ATPPROSITE-ProRule annotation
    Nucleotide bindingi734 – 7396ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell projection organization Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Cilium biogenesis/degradation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase, flagellar (EC:2.7.3.2)
    OrganismiStrongylocentrotus purpuratus (Purple sea urchin)
    Taxonomic identifieri7668 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEchinodermataEleutherozoaEchinozoaEchinoideaEuechinoideaEchinaceaEchinoidaStrongylocentrotidaeStrongylocentrotus
    ProteomesiUP000007110: Unplaced

    Subcellular locationi

    Cytoplasmcytoskeletonflagellum axoneme
    Note: Associates specifically with the axoneme and may bind directly to polymerized microtubules.

    GO - Cellular componenti

    1. cilium Source: UniProtKB-KW
    2. cytoplasm Source: UniProtKB-KW
    3. cytoskeleton Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11741174Creatine kinase, flagellarPRO_0000212010Add
    BLAST

    Proteomic databases

    PRIDEiP18294.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi7668.P18294.

    Structurei

    3D structure databases

    ProteinModelPortaliP18294.
    SMRiP18294. Positions 61-419, 431-794, 807-1168.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini53 – 13987Phosphagen kinase N-terminal 1PROSITE-ProRule annotationAdd
    BLAST
    Repeati61 – 4143541; approximateAdd
    BLAST
    Domaini166 – 408243Phosphagen kinase C-terminal 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini426 – 51287Phosphagen kinase N-terminal 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati434 – 7873542; approximateAdd
    BLAST
    Domaini539 – 781243Phosphagen kinase C-terminal 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini800 – 88687Phosphagen kinase N-terminal 3PROSITE-ProRule annotationAdd
    BLAST
    Repeati808 – 11613543; approximateAdd
    BLAST
    Domaini913 – 1155243Phosphagen kinase C-terminal 3PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Contains three complete but non-identical creatine kinase segments flanked by unique regions.

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 3 phosphagen kinase C-terminal domains.PROSITE-ProRule annotation
    Contains 3 phosphagen kinase N-terminal domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG3869.
    InParanoidiP18294.
    KOiK00933.

    Family and domain databases

    Gene3Di1.10.135.10. 3 hits.
    3.30.590.10. 3 hits.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 3 hits.
    PF02807. ATP-gua_PtransN. 3 hits.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 3 hits.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 3 hits.
    PS51510. PHOSPHAGEN_KINASE_C. 3 hits.
    PS51509. PHOSPHAGEN_KINASE_N. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18294-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCAASSQQT TATGGQPAAG EKANPAPANN NPNAANKAET TGAAEELTKE     50
    SEPFVEPDPN YPDLSKHNNY LAESLTPSIY NKICNLRTLS GYSVDGCMQT 100
    GVDNPGHPFI KTVGLVAGDE ECYDLFADLF DPTIDKRHNG YPRNAKHTTD 150
    LNPDHLKGGD DFDPKYVLSC RVRTGRCIRG YGLPPHCTRA ERRDVEKVCK 200
    DALATLDGPL KGTYYPLTGM TEEMQDKLIA DHFLFDKPVS PLLMSARMAR 250
    DWPDGRGIWH NADKNFLVWI NEEDHTRVIS METSGNMKNV FKRFCNGLNK 300
    VENALKAKGY EFSWNEHLGY VLTCPSNLGT GVRAGVHIKI PLFSKHAGFE 350
    SILKHYRLQK RGTGGVDTAS TDGTFDISNL DRLGTSEVQQ VQSVVDGVKK 400
    LIELEKALEK GSDISGQIPR DPAIVRAEQV KEGYPDLSKH NNHLAHCLTY 450
    DIWKSLKDKK TPSGFTLDGC IQTGVMNPGH PHIMTVGMVA GDEESYDVFA 500
    DIFDPVIDAR HGGYPKDAVH VTNINHADLK GGDNLDPKYV LSCRVRTGRS 550
    IIGYSLPPHC TVEERAAVET ITIGALDKFD GDLQGKYYPL EGMSDETQTQ 600
    LIDDHFLFDK PVSPLLTAAR MHRDWPQGRG IWHNENKNFL VWVNEEDHIR 650
    VISMEKDGNM RAVFKRFCEG LQKFEQMIKK DGKEFMWNKH LGYVLTCPSN 700
    LGTGLRAGVH VKLPLLSKYP RFDQILRALR LQKRGTGGVD TASTDGTFDI 750
    SNLDRLGSSE VQQVQFVVDG VELLVQMEKK LEKGEDIFDI LPQQCRPKPP 800
    IKPFSYDYPD FSLHNNWMSK CMTEEIYNKL CNLKTKGGVT LNDCIQTGID 850
    NPGHPYIMTV GLVAGDEECY EVFAPLFDPV ISARHGGYAL DAKHPTNLNA 900
    AELKGGDDLD PEFVLSCRVR TGRCIRGLAL PPCCTRAERA EVEKITTEAL 950
    STLSGPLKGK YYPLTGMTDE EQEKLIEDHF LFDKPVSPLL LCANMARDWP 1000
    QGRGIWHNDE KNFLVWVNEE DHTRVISMEK SGNMKRVFER FCDGLKKVED 1050
    SIKSKGYQFM WNEHLGYVLT CPSNLGTGLR AGVHVKVPLL SQQKIFDSIL 1100
    DHMRLQKRGT GGVDTASTDG TFDISNSDRI GFSEVHLVQQ LVDGVKLLVN 1150
    LEKALMKGED INSLLPEKLR EDSS 1174
    Length:1,174
    Mass (Da):130,869
    Last modified:November 1, 1990 - v1
    Checksum:iB8F4FBA5AC48EE93
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33763 mRNA. Translation: AAA30049.1.
    PIRiA43736.
    RefSeqiNP_999687.1. NM_214522.1.
    UniGeneiSpu.13.

    Genome annotation databases

    GeneIDi373287.
    KEGGispu:373287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33763 mRNA. Translation: AAA30049.1 .
    PIRi A43736.
    RefSeqi NP_999687.1. NM_214522.1.
    UniGenei Spu.13.

    3D structure databases

    ProteinModelPortali P18294.
    SMRi P18294. Positions 61-419, 431-794, 807-1168.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7668.P18294.

    Proteomic databases

    PRIDEi P18294.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 373287.
    KEGGi spu:373287.

    Organism-specific databases

    CTDi 373287.

    Phylogenomic databases

    eggNOGi COG3869.
    InParanoidi P18294.
    KOi K00933.

    Family and domain databases

    Gene3Di 1.10.135.10. 3 hits.
    3.30.590.10. 3 hits.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 3 hits.
    PF02807. ATP-gua_PtransN. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 3 hits.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 3 hits.
    PS51510. PHOSPHAGEN_KINASE_C. 3 hits.
    PS51509. PHOSPHAGEN_KINASE_N. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication."
      Wothe D.D., Charbonneau H., Shapiro B.M.
      Proc. Natl. Acad. Sci. U.S.A. 87:5203-5207(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Testis.

    Entry informationi

    Entry nameiKCRF_STRPU
    AccessioniPrimary (citable) accession number: P18294
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3