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Protein

Creatine kinase, flagellar

Gene
N/A
Organism
Strongylocentrotus purpuratus (Purple sea urchin)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This axonemal protein participates in an energy shuttle that utilizes phosphocreatine to transfer the energy from ATP generated by the mitochondrion in the sperm head to dynein in the distal portions of the flagellum.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei232 – 2321ATPPROSITE-ProRule annotation
Binding sitei277 – 2771ATPPROSITE-ProRule annotation
Binding sitei605 – 6051ATPPROSITE-ProRule annotation
Binding sitei706 – 7061ATPPROSITE-ProRule annotation
Binding sitei749 – 7491ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1735ATPPROSITE-ProRule annotation
Nucleotide bindingi333 – 3375ATPPROSITE-ProRule annotation
Nucleotide bindingi542 – 5465ATPPROSITE-ProRule annotation
Nucleotide bindingi734 – 7396ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase, flagellar (EC:2.7.3.2)
OrganismiStrongylocentrotus purpuratus (Purple sea urchin)
Taxonomic identifieri7668 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEchinodermataEleutherozoaEchinozoaEchinoideaEuechinoideaEchinaceaEchinoidaStrongylocentrotidaeStrongylocentrotus
ProteomesiUP000007110 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11741174Creatine kinase, flagellarPRO_0000212010Add
BLAST

Proteomic databases

PRIDEiP18294.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi7668.SPU_014785tr.

Structurei

3D structure databases

ProteinModelPortaliP18294.
SMRiP18294. Positions 61-419, 431-794, 807-1168.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 13987Phosphagen kinase N-terminal 1PROSITE-ProRule annotationAdd
BLAST
Repeati61 – 4143541; approximateAdd
BLAST
Domaini166 – 408243Phosphagen kinase C-terminal 1PROSITE-ProRule annotationAdd
BLAST
Domaini426 – 51287Phosphagen kinase N-terminal 2PROSITE-ProRule annotationAdd
BLAST
Repeati434 – 7873542; approximateAdd
BLAST
Domaini539 – 781243Phosphagen kinase C-terminal 2PROSITE-ProRule annotationAdd
BLAST
Domaini800 – 88687Phosphagen kinase N-terminal 3PROSITE-ProRule annotationAdd
BLAST
Repeati808 – 11613543; approximateAdd
BLAST
Domaini913 – 1155243Phosphagen kinase C-terminal 3PROSITE-ProRule annotationAdd
BLAST

Domaini

Contains three complete but non-identical creatine kinase segments flanked by unique regions.

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 3 phosphagen kinase C-terminal domains.PROSITE-ProRule annotation
Contains 3 phosphagen kinase N-terminal domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG3869.
KOiK00933.

Family and domain databases

Gene3Di1.10.135.10. 3 hits.
3.30.590.10. 3 hits.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 3 hits.
PF02807. ATP-gua_PtransN. 3 hits.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 3 hits.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 3 hits.
PS51510. PHOSPHAGEN_KINASE_C. 3 hits.
PS51509. PHOSPHAGEN_KINASE_N. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCAASSQQT TATGGQPAAG EKANPAPANN NPNAANKAET TGAAEELTKE
60 70 80 90 100
SEPFVEPDPN YPDLSKHNNY LAESLTPSIY NKICNLRTLS GYSVDGCMQT
110 120 130 140 150
GVDNPGHPFI KTVGLVAGDE ECYDLFADLF DPTIDKRHNG YPRNAKHTTD
160 170 180 190 200
LNPDHLKGGD DFDPKYVLSC RVRTGRCIRG YGLPPHCTRA ERRDVEKVCK
210 220 230 240 250
DALATLDGPL KGTYYPLTGM TEEMQDKLIA DHFLFDKPVS PLLMSARMAR
260 270 280 290 300
DWPDGRGIWH NADKNFLVWI NEEDHTRVIS METSGNMKNV FKRFCNGLNK
310 320 330 340 350
VENALKAKGY EFSWNEHLGY VLTCPSNLGT GVRAGVHIKI PLFSKHAGFE
360 370 380 390 400
SILKHYRLQK RGTGGVDTAS TDGTFDISNL DRLGTSEVQQ VQSVVDGVKK
410 420 430 440 450
LIELEKALEK GSDISGQIPR DPAIVRAEQV KEGYPDLSKH NNHLAHCLTY
460 470 480 490 500
DIWKSLKDKK TPSGFTLDGC IQTGVMNPGH PHIMTVGMVA GDEESYDVFA
510 520 530 540 550
DIFDPVIDAR HGGYPKDAVH VTNINHADLK GGDNLDPKYV LSCRVRTGRS
560 570 580 590 600
IIGYSLPPHC TVEERAAVET ITIGALDKFD GDLQGKYYPL EGMSDETQTQ
610 620 630 640 650
LIDDHFLFDK PVSPLLTAAR MHRDWPQGRG IWHNENKNFL VWVNEEDHIR
660 670 680 690 700
VISMEKDGNM RAVFKRFCEG LQKFEQMIKK DGKEFMWNKH LGYVLTCPSN
710 720 730 740 750
LGTGLRAGVH VKLPLLSKYP RFDQILRALR LQKRGTGGVD TASTDGTFDI
760 770 780 790 800
SNLDRLGSSE VQQVQFVVDG VELLVQMEKK LEKGEDIFDI LPQQCRPKPP
810 820 830 840 850
IKPFSYDYPD FSLHNNWMSK CMTEEIYNKL CNLKTKGGVT LNDCIQTGID
860 870 880 890 900
NPGHPYIMTV GLVAGDEECY EVFAPLFDPV ISARHGGYAL DAKHPTNLNA
910 920 930 940 950
AELKGGDDLD PEFVLSCRVR TGRCIRGLAL PPCCTRAERA EVEKITTEAL
960 970 980 990 1000
STLSGPLKGK YYPLTGMTDE EQEKLIEDHF LFDKPVSPLL LCANMARDWP
1010 1020 1030 1040 1050
QGRGIWHNDE KNFLVWVNEE DHTRVISMEK SGNMKRVFER FCDGLKKVED
1060 1070 1080 1090 1100
SIKSKGYQFM WNEHLGYVLT CPSNLGTGLR AGVHVKVPLL SQQKIFDSIL
1110 1120 1130 1140 1150
DHMRLQKRGT GGVDTASTDG TFDISNSDRI GFSEVHLVQQ LVDGVKLLVN
1160 1170
LEKALMKGED INSLLPEKLR EDSS
Length:1,174
Mass (Da):130,869
Last modified:November 1, 1990 - v1
Checksum:iB8F4FBA5AC48EE93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33763 mRNA. Translation: AAA30049.1.
PIRiA43736.
RefSeqiNP_999687.1. NM_214522.1.
UniGeneiSpu.13.

Genome annotation databases

GeneIDi373287.
KEGGispu:373287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33763 mRNA. Translation: AAA30049.1.
PIRiA43736.
RefSeqiNP_999687.1. NM_214522.1.
UniGeneiSpu.13.

3D structure databases

ProteinModelPortaliP18294.
SMRiP18294. Positions 61-419, 431-794, 807-1168.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7668.SPU_014785tr.

Proteomic databases

PRIDEiP18294.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi373287.
KEGGispu:373287.

Organism-specific databases

CTDi373287.

Phylogenomic databases

eggNOGiCOG3869.
KOiK00933.

Family and domain databases

Gene3Di1.10.135.10. 3 hits.
3.30.590.10. 3 hits.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 3 hits.
PF02807. ATP-gua_PtransN. 3 hits.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 3 hits.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 3 hits.
PS51510. PHOSPHAGEN_KINASE_C. 3 hits.
PS51509. PHOSPHAGEN_KINASE_N. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication."
    Wothe D.D., Charbonneau H., Shapiro B.M.
    Proc. Natl. Acad. Sci. U.S.A. 87:5203-5207(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Testis.

Entry informationi

Entry nameiKCRF_STRPU
AccessioniPrimary (citable) accession number: P18294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 24, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.