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Protein

Atrial natriuretic peptide receptor 1

Gene

Npr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81ChlorideBy similarity1
Binding sitei113Chloride; via amide nitrogenBy similarity1
Binding sitei114Chloride; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Receptor, Vasoactive
Biological processcGMP biosynthesis
LigandChloride, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2 3474
ReactomeiR-MMU-5578768 Physiological factors

Names & Taxonomyi

Protein namesi
Recommended name:
Atrial natriuretic peptide receptor 1 (EC:4.6.1.2)
Alternative name(s):
Atrial natriuretic peptide receptor type A
Short name:
ANP-A
Short name:
ANPR-A
Short name:
NPR-A
Guanylate cyclase A
Short name:
GC-A
Gene namesi
Name:Npr1
Synonyms:Npra
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:97371 Npr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 469ExtracellularSequence analysisAdd BLAST441
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 1057CytoplasmicSequence analysisAdd BLAST567

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display systemic hypertension associated with cardiac hypertrophy and ventricular enlargement.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Add BLAST28
ChainiPRO_000001236129 – 1057Atrial natriuretic peptide receptor 1Add BLAST1029

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi41N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi88 ↔ 114By similarity
Disulfide bondi192 ↔ 241By similarity
Glycosylationi208N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi334N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi375N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi382N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi423N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi451 ↔ 460By similarity
Modified residuei515PhosphoserineBy similarity1
Modified residuei525PhosphoserineBy similarity1
Modified residuei528PhosphothreonineBy similarity1
Modified residuei530PhosphoserineBy similarity1
Modified residuei534PhosphoserineBy similarity1
Modified residuei538PhosphoserineBy similarity1
Modified residuei541PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation of the protein kinase-like domain is required for full activation by ANP.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP18293
PRIDEiP18293

PTM databases

iPTMnetiP18293
PhosphoSitePlusiP18293

Expressioni

Gene expression databases

BgeeiENSMUSG00000027931
CleanExiMM_NPR1
ExpressionAtlasiP18293 baseline and differential
GenevisibleiP18293 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi201830, 3 interactors
STRINGi10090.ENSMUSP00000029540

Structurei

3D structure databases

ProteinModelPortaliP18293
SMRiP18293
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini524 – 801Protein kinasePROSITE-ProRule annotationAdd BLAST278
Domaini872 – 1002Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000293307
HOVERGENiHBG051862
InParanoidiP18293
KOiK12323
OMAiREDPSML
OrthoDBiEOG091G02QS
PhylomeDBiP18293
TreeFamiTF106338

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR001828 ANF_lig-bd_rcpt
IPR001170 ANPR/GUC
IPR011009 Kinase-like_dom_sf
IPR029787 Nucleotide_cyclase
IPR028082 Peripla_BP_I
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF01094 ANF_receptor, 1 hit
PF00211 Guanylate_cyc, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00255 NATPEPTIDER
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit
SSF55073 SSF55073, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00458 ANF_RECEPTORS, 1 hit
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGSRRVRPR LRALLLLPPL LLLRSGHASD LTVAVVLPLT NTSYPWSWAR
60 70 80 90 100
VGPAVELALG RVKARPDLLP GWTVRMVLGS SENAAGVCSD TAAPLAAVDL
110 120 130 140 150
KWEHSPAVFL GPGCVYSAAP VGRFTAHWRV PLLTAGAPAL GIGVKDEYAL
160 170 180 190 200
TTRTGPSHVK LGDFVTALHR RLGWEHQALV LYADRLGDDR PCFFIVEGLY
210 220 230 240 250
MRVRERLNIT VNHQEFVEGD PDHYTKLLRT VQRKGRVIYI CSSPDAFRNL
260 270 280 290 300
MLLALDAGLT GEDYVFFHLD VFGQSLQGAQ GPVPRKPWER DDGQDRRARQ
310 320 330 340 350
AFQAAKIITY KEPDNPEYLE FLKQLKLLAD KKFNFTMEDG LKNIIPASFH
360 370 380 390 400
DGLLLYVQAV TETLAQGGTV TDGENITQRM WNRSFQGVTG YLKIDRNGDR
410 420 430 440 450
DTDFSLWDMD PETGAFRVVL NFNGTSQELM AVSEHRLYWP LGYPPPDIPK
460 470 480 490 500
CGFDNEDPAC NQDHFSTLEV LALVGSLSLV SFLIVSFFIY RKMQLEKELV
510 520 530 540 550
SELWRVRWED LQPSSLERHL RSAGSRLTLS GRGSNYGSLL TTEGQFQVFA
560 570 580 590 600
KTAYYKGNLV AVKRVNRKRI ELTRKVLFEL KHMRDVQNEH LTRFVGACTD
610 620 630 640 650
PPNICILTEY CPRGSLQDIL ENESITLDWM FRYSLTNDIV KGMLFLHNGA
660 670 680 690 700
IGSHGNLKSS NCVVDGRFVL KITDYGLESF RDPEPEQGHT LFAKKLWTAP
710 720 730 740 750
ELLRMASPPA RGSQAGDVYS FGIILQEIAL RSGVFYVEGL DLSPKEIIER
760 770 780 790 800
VTRGEQPPFR PSMDLQSHLE ELGQLMQRCW AEDPQERPPF QQIRLALRKF
810 820 830 840 850
NKENSSNILD NLLSRMEQYA NNLEELVEER TQAYLEEKRK AEALLYQILP
860 870 880 890 900
HSVAEQLKRG ETVQAEAFDS VTIYFSDIVG FTALSAESTP MQVVTLLNDL
910 920 930 940 950
YTCFDAVIDN FDVYKVETIG DAYMVVSGLP VRNGQLHARE VARMALALLD
960 970 980 990 1000
AVRSFRIRHR PQEQLRLRIG IHTGPVCAGV VGLKMPRYCL FGDTVNTASR
1010 1020 1030 1040 1050
MESNGEALRI HLSSETKAVL EEFDGFELEL RGDVEMKGKG KVRTYWLLGE

RGCSTRG
Length:1,057
Mass (Da):119,109
Last modified:February 1, 1995 - v2
Checksum:i53A544FB2C8EF253
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3G → R in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti39L → V in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti122G → D in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti130V → L in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti285R → E in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti301A → R in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti404 – 405FS → SP in AAA37670 (PubMed:1973687).Curated2
Sequence conflicti590H → Q in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti652G → C in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti833A → P in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti958R → G in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti1044T → S in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti1050E → D in AAA37670 (PubMed:1973687).Curated1
Sequence conflicti1055 – 1057TRG → SRA in AAA37670 (PubMed:1973687).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05504 mRNA Translation: AAA37670.1
L31932 mRNA Translation: AAA66945.1
CCDSiCCDS17529.1
PIRiA36568 OYMSAR
I57963
RefSeqiNP_032753.5, NM_008727.5
UniGeneiMm.4627

Genome annotation databases

EnsembliENSMUST00000029540; ENSMUSP00000029540; ENSMUSG00000027931
GeneIDi18160
KEGGimmu:18160
UCSCiuc008qcg.1 mouse

Similar proteinsi

Entry informationi

Entry nameiANPRA_MOUSE
AccessioniPrimary (citable) accession number: P18293
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health