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Protein

Atrial natriuretic peptide receptor 1

Gene

Npr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811ChlorideBy similarity
Binding sitei113 – 1131Chloride; via amide nitrogenBy similarity
Binding sitei114 – 1141Chloride; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor, Vasoactive

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

Chloride, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2. 3474.
ReactomeiR-MMU-5578768. Physiological factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Atrial natriuretic peptide receptor 1 (EC:4.6.1.2)
Alternative name(s):
Atrial natriuretic peptide receptor type A
Short name:
ANP-A
Short name:
ANPR-A
Short name:
NPR-A
Guanylate cyclase A
Short name:
GC-A
Gene namesi
Name:Npr1
Synonyms:Npra
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:97371. Npr1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 469441ExtracellularSequence analysisAdd
BLAST
Transmembranei470 – 49021HelicalSequence analysisAdd
BLAST
Topological domaini491 – 1057567CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display systemic hypertension associated with cardiac hypertrophy and ventricular enlargement.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Add
BLAST
Chaini29 – 10571029Atrial natriuretic peptide receptor 1PRO_0000012361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence analysis
Disulfide bondi88 ↔ 114By similarity
Disulfide bondi192 ↔ 241By similarity
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence analysis
Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence analysis
Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence analysis
Glycosylationi382 – 3821N-linked (GlcNAc...)Sequence analysis
Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence analysis
Disulfide bondi451 ↔ 460By similarity
Modified residuei515 – 5151PhosphoserineBy similarity
Modified residuei525 – 5251PhosphoserineBy similarity
Modified residuei528 – 5281PhosphothreonineBy similarity
Modified residuei530 – 5301PhosphoserineBy similarity
Modified residuei534 – 5341PhosphoserineBy similarity
Modified residuei538 – 5381PhosphoserineBy similarity
Modified residuei541 – 5411PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylation of the protein kinase-like domain is required for full activation by ANP.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP18293.
PaxDbiP18293.
PRIDEiP18293.

PTM databases

iPTMnetiP18293.
PhosphoSiteiP18293.

Expressioni

Gene expression databases

BgeeiP18293.
CleanExiMM_NPR1.
ExpressionAtlasiP18293. baseline and differential.
GenevisibleiP18293. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi201830. 2 interactions.
STRINGi10090.ENSMUSP00000029540.

Structurei

3D structure databases

ProteinModelPortaliP18293.
SMRiP18293. Positions 29-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini524 – 801278Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini872 – 1002131Guanylate cyclasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiP18293.
KOiK12323.
OMAiCFFLVEG.
OrthoDBiEOG7Z69BJ.
PhylomeDBiP18293.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR001170. ANPR/GUC.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGSRRVRPR LRALLLLPPL LLLRSGHASD LTVAVVLPLT NTSYPWSWAR
60 70 80 90 100
VGPAVELALG RVKARPDLLP GWTVRMVLGS SENAAGVCSD TAAPLAAVDL
110 120 130 140 150
KWEHSPAVFL GPGCVYSAAP VGRFTAHWRV PLLTAGAPAL GIGVKDEYAL
160 170 180 190 200
TTRTGPSHVK LGDFVTALHR RLGWEHQALV LYADRLGDDR PCFFIVEGLY
210 220 230 240 250
MRVRERLNIT VNHQEFVEGD PDHYTKLLRT VQRKGRVIYI CSSPDAFRNL
260 270 280 290 300
MLLALDAGLT GEDYVFFHLD VFGQSLQGAQ GPVPRKPWER DDGQDRRARQ
310 320 330 340 350
AFQAAKIITY KEPDNPEYLE FLKQLKLLAD KKFNFTMEDG LKNIIPASFH
360 370 380 390 400
DGLLLYVQAV TETLAQGGTV TDGENITQRM WNRSFQGVTG YLKIDRNGDR
410 420 430 440 450
DTDFSLWDMD PETGAFRVVL NFNGTSQELM AVSEHRLYWP LGYPPPDIPK
460 470 480 490 500
CGFDNEDPAC NQDHFSTLEV LALVGSLSLV SFLIVSFFIY RKMQLEKELV
510 520 530 540 550
SELWRVRWED LQPSSLERHL RSAGSRLTLS GRGSNYGSLL TTEGQFQVFA
560 570 580 590 600
KTAYYKGNLV AVKRVNRKRI ELTRKVLFEL KHMRDVQNEH LTRFVGACTD
610 620 630 640 650
PPNICILTEY CPRGSLQDIL ENESITLDWM FRYSLTNDIV KGMLFLHNGA
660 670 680 690 700
IGSHGNLKSS NCVVDGRFVL KITDYGLESF RDPEPEQGHT LFAKKLWTAP
710 720 730 740 750
ELLRMASPPA RGSQAGDVYS FGIILQEIAL RSGVFYVEGL DLSPKEIIER
760 770 780 790 800
VTRGEQPPFR PSMDLQSHLE ELGQLMQRCW AEDPQERPPF QQIRLALRKF
810 820 830 840 850
NKENSSNILD NLLSRMEQYA NNLEELVEER TQAYLEEKRK AEALLYQILP
860 870 880 890 900
HSVAEQLKRG ETVQAEAFDS VTIYFSDIVG FTALSAESTP MQVVTLLNDL
910 920 930 940 950
YTCFDAVIDN FDVYKVETIG DAYMVVSGLP VRNGQLHARE VARMALALLD
960 970 980 990 1000
AVRSFRIRHR PQEQLRLRIG IHTGPVCAGV VGLKMPRYCL FGDTVNTASR
1010 1020 1030 1040 1050
MESNGEALRI HLSSETKAVL EEFDGFELEL RGDVEMKGKG KVRTYWLLGE

RGCSTRG
Length:1,057
Mass (Da):119,109
Last modified:February 1, 1995 - v2
Checksum:i53A544FB2C8EF253
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31G → R in AAA37670 (PubMed:1973687).Curated
Sequence conflicti39 – 391L → V in AAA37670 (PubMed:1973687).Curated
Sequence conflicti122 – 1221G → D in AAA37670 (PubMed:1973687).Curated
Sequence conflicti130 – 1301V → L in AAA37670 (PubMed:1973687).Curated
Sequence conflicti285 – 2851R → E in AAA37670 (PubMed:1973687).Curated
Sequence conflicti301 – 3011A → R in AAA37670 (PubMed:1973687).Curated
Sequence conflicti404 – 4052FS → SP in AAA37670 (PubMed:1973687).Curated
Sequence conflicti590 – 5901H → Q in AAA37670 (PubMed:1973687).Curated
Sequence conflicti652 – 6521G → C in AAA37670 (PubMed:1973687).Curated
Sequence conflicti833 – 8331A → P in AAA37670 (PubMed:1973687).Curated
Sequence conflicti958 – 9581R → G in AAA37670 (PubMed:1973687).Curated
Sequence conflicti1044 – 10441T → S in AAA37670 (PubMed:1973687).Curated
Sequence conflicti1050 – 10501E → D in AAA37670 (PubMed:1973687).Curated
Sequence conflicti1055 – 10573TRG → SRA in AAA37670 (PubMed:1973687).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05504 mRNA. Translation: AAA37670.1.
L31932 mRNA. Translation: AAA66945.1.
CCDSiCCDS17529.1.
PIRiA36568. OYMSAR.
I57963.
RefSeqiNP_032753.5. NM_008727.5.
UniGeneiMm.4627.

Genome annotation databases

EnsembliENSMUST00000029540; ENSMUSP00000029540; ENSMUSG00000027931.
GeneIDi18160.
KEGGimmu:18160.
UCSCiuc008qcg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05504 mRNA. Translation: AAA37670.1.
L31932 mRNA. Translation: AAA66945.1.
CCDSiCCDS17529.1.
PIRiA36568. OYMSAR.
I57963.
RefSeqiNP_032753.5. NM_008727.5.
UniGeneiMm.4627.

3D structure databases

ProteinModelPortaliP18293.
SMRiP18293. Positions 29-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201830. 2 interactions.
STRINGi10090.ENSMUSP00000029540.

PTM databases

iPTMnetiP18293.
PhosphoSiteiP18293.

Proteomic databases

EPDiP18293.
PaxDbiP18293.
PRIDEiP18293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029540; ENSMUSP00000029540; ENSMUSG00000027931.
GeneIDi18160.
KEGGimmu:18160.
UCSCiuc008qcg.1. mouse.

Organism-specific databases

CTDi4881.
MGIiMGI:97371. Npr1.

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiP18293.
KOiK12323.
OMAiCFFLVEG.
OrthoDBiEOG7Z69BJ.
PhylomeDBiP18293.
TreeFamiTF106338.

Enzyme and pathway databases

BRENDAi4.6.1.2. 3474.
ReactomeiR-MMU-5578768. Physiological factors.

Miscellaneous databases

ChiTaRSiNpr1. mouse.
PROiP18293.
SOURCEiSearch...

Gene expression databases

BgeeiP18293.
CleanExiMM_NPR1.
ExpressionAtlasiP18293. baseline and differential.
GenevisibleiP18293. MM.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR001170. ANPR/GUC.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of murine guanylate cyclase/atrial natriuretic factor receptor cDNA."
    Pandey K.N., Singh S.
    J. Biol. Chem. 265:12342-12348(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "Agonist selectivity for three species of natriuretic peptide receptor-A."
    Schoenfeld J.R., Sehl P., Quan C., Burnier J.P., Lowe D.G.
    Mol. Pharmacol. 47:172-180(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Hypertension, cardiac hypertrophy, and sudden death in mice lacking natriuretic peptide receptor A."
    Oliver P.M., Fox J.E., Kim R., Rockman H.A., Kim H.S., Reddick R.L., Pandey K.N., Milgram S.L., Smithies O., Maeda N.
    Proc. Natl. Acad. Sci. U.S.A. 94:14730-14735(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Liver, Lung and Testis.

Entry informationi

Entry nameiANPRA_MOUSE
AccessioniPrimary (citable) accession number: P18293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.