P18289 (JRA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor AP-1

Alternative name(s):
Jun-related antigen
dJRA
dJun

Gene names

Name:	Jra
Synonyms:	jun
ORF Names:	CG2275

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
Subunit structure	AP-1/dJRA interacts with AP-1/dFra to form a dimer. The dFra-dJRA complex is bound more stably to the AP-1 site than either of the two proteins alone.
Subcellular location	Nucleus.
Developmental stage	Expressed during embryonic development.
Sequence similarities	Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	JNK cascade Inferred from mutant phenotype PubMed 9224721. Source: FlyBase R3/R4 cell fate commitment Traceable author statement PubMed 12414186. Source: FlyBase R7 cell fate commitment Traceable author statement PubMed 8946916. Source: FlyBase dorsal appendage formation Inferred from mutant phenotype PubMed 11543614. Source: FlyBase dorsal closure Non-traceable author statement PubMed 12000787. Source: FlyBase imaginal disc fusion, thorax closure Inferred from mutant phenotype PubMed 16930585. Source: FlyBase micropyle formation Inferred from mutant phenotype PubMed 11543614. Source: FlyBase negative regulation of antimicrobial humoral response Inferred from mutant phenotype PubMed 17803358. Source: FlyBase phagocytosis, engulfment Inferred from mutant phenotype PubMed 16336044. Source: FlyBase positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.2. Source: FlyBase synaptic growth at neuromuscular junction Inferred from genetic interaction PubMed 18832361. Source: FlyBase wound healing Inferred from mutant phenotype PubMed 19884309. Source: FlyBase
Cellular_component	cytoplasm Inferred from direct assay PubMed 11509232. Source: FlyBase transcription factor complex Inferred from physical interaction Ref.2. Source: FlyBase
Molecular_function	protein heterodimerization activity Inferred from sequence or structural similarity PubMed 12176927. Source: FlyBase sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from direct assay Ref.2. Source: FlyBase transcription factor binding Traceable author statement PubMed 12000787. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Transcription factor AP-1		PRO_0000076437
Regions
	Domain	212 – 275	64	bZIP
	Region	212 – 239	28	Basic motif By similarity
	Region	240 – 268	29	Leucine-zipper By similarity
Amino acid modifications
	Modified residue	82	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	267 – 268	2	QL → HV in AAA28650. Ref.1
	Sequence conflict	267 – 268	2	QL → HV in CAA73154. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P18289 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: FCCA13A6D2AF32BF	FASTA	289	31,021
	10 20 30 40 50 60 MKTPVSAAAN LSIQNAGSSG ATAIQIIPKT EPVGEEGPMS LDFQSPNLNT STPNPNKRPG 70 80 90 100 110 120 SLDLNSKSAK NKRIFAPLVI NSPDLSSKTV NTPDLEKILL SNNLMQTPQP GKVFPTKAGP 130 140 150 160 170 180 VTVEQLDFGR GFEEALHNLH TNSQAFPSAN SAANSAANNT TAAAMTAVNN GISGGTFTYT 190 200 210 220 230 240 NMTEGFSVIK DEPVNQASSP TVNPIDMEAQ EKIKLERKRQ RNRVAASKCR KRKLERISKL 250 260 270 280 EDRVKVLKGE NVDLASIVKN LKDHVAQLKQ QVMEHIAAGC TVPPNSTDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila homolog of the mammalian jun oncogene is expressed during embryonic development and activates transcription in mammalian cells." Zhang K., Chaillet J.R., Perkins L.A., Halazonetis T.D., Perrimon N. Proc. Natl. Acad. Sci. U.S.A. 87:6281-6285(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Oregon-R.
[2]	"The Drosophila Fos-related AP-1 protein is a developmentally regulated transcription factor." Perkins K.K., Admon A., Patel N., Tjian R. Genes Dev. 4:822-834(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Embryo.
[3]	"Jun in Drosophila development: redundant and nonredundant functions and regulation by two MAPK signal transduction pathways." Kockel L., Zeitlinger J., Staszewski L., Mlodzik M., Bohmann D. Genes Dev. 11:1748-1758(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Oregon-R.
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[7]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36181 Genomic DNA. Translation: AAA28650.1. X54144 mRNA. Translation: CAA38083.1. Y12573 Genomic DNA. Translation: CAA73154.1. AE013599 Genomic DNA. Translation: AAF58845.1. AY058562 mRNA. Translation: AAL13791.1.
PIR	TVFFJD. A36011.
RefSeq	NP_476586.1. NM_057238.4. NP_724882.1. NM_165739.2.
UniGene	Dm.3574.
3D structure databases
ProteinModelPortal	P18289.
SMR	P18289. Positions 216-273.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-18982N.
IntAct	P18289. 8 interactions.
MINT	MINT-278412.
Proteomic databases
PaxDb	P18289.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0088410; FBpp0087498; FBgn0001291. FBtr0088411; FBpp0087499; FBgn0001291.
GeneID	36057.
KEGG	dme:Dmel_CG2275.
Organism-specific databases
CTD	36057.
FlyBase	FBgn0001291. Jra.
Phylogenomic databases
eggNOG	NOG283376.
GeneTree	ENSGT00390000009929.
HOGENOM	HOG000047089.
InParanoid	P18289.
OMA	KDHVAQL.
OrthoDB	EOG4KWH8S.
PhylomeDB	P18289.
Gene expression databases
Bgee	P18289.
GermOnline	CG2275. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.880.10. 1 hit.
InterPro	IPR004827. bZIP. IPR008917. Euk_TF_DNA-bd. IPR005643. JNK. IPR002112. Leuzip_Jun. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. PF03957. Jun. 1 hit. [Graphical view]
PRINTS	PR00043. LEUZIPPRJUN.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	36057.
NextBio	796620.

Entry information

Entry name

JRA_DROME

Accession

Primary (citable) accession number: P18289
Secondary accession number(s): Q0E9D8, Q9V5G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 1, 2000
Last modified:	April 3, 2013
This is version 141 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project