Reviewed, UniProtKB/Swiss-Prot P18278 (DHET_ACEAC)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase [acceptor]
    EC=1.1.99.8

Gene names

Name:	adhA
Synonyms:	adh1

Organism

Acetobacter aceti

Taxonomic identifier

435 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acetobacter › Acetobacter subgen. Acetobacter

Protein attributes

Sequence length	742 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A primary alcohol + acceptor = an aldehyde + reduced acceptor.
Cofactor	Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-142-Cys-143 and the indole ring of Trp-280 By similarity. Binds 1 calcium ion per subunit By similarity. Binds 1 heme group per subunit By similarity.
Subunit structure	Tetramer of non identical chains (dehydrogenase, cytochrome, and two smaller unknown subunits).
Subcellular location	Periplasm.
Sequence similarities	Belongs to the bacterial PQQ dehydrogenase family. Contains 1 cytochrome c domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond PQQ
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: InterPro outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	alcohol dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Ref.1
	Chain	36 – 742	707	Alcohol dehydrogenase [acceptor]		PRO_0000025559
Regions
	Domain	633 – 742	110	Cytochrome c
Sites
	Active site	343	1	Proton acceptor Potential
	Metal binding	216	1	Calcium By similarity
	Metal binding	298	1	Calcium By similarity
	Metal binding	653	1	Iron (heme axial ligand) By similarity
	Binding site	649	1	Heme (covalent) By similarity
	Binding site	652	1	Heme (covalent) By similarity
Amino acid modifications
	Disulfide bond	142 ↔ 143		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18278-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 9C6C9268DABB825A	FASTA	742	81,521
	10 20 30 40 50 60 MTRPASAKRR SLLGILAAGT ICAAALPYAA VPARADGQGN TGEAIIHADD HPENWLSYGR 70 80 90 100 110 120 TYSEQRYSPL DQINRSNVGD LKLLGYYTLD TNRGQEATPL VVDGIMYATT NWSKMEALDA 130 140 150 160 170 180 ATGKLLWQYD PKVPGNIADK GCCDTVNRGA GYWNGKVFWG TFDGRLVAAD AKTGKKVWAV 190 200 210 220 230 240 NTIPADASLG KQRSYTVDGA VRVAKGLVLI GNGGAEFGAR GFVSAFDAET GKLKWRFYTV 250 260 270 280 290 300 PNNKNEPDHA ASDNILMNKA YKTWGPKGAW VRQGGGGTVW DSLVYDPVSD LIYLAVGNGS 310 320 330 340 350 360 PWNYKYRSEG IGSNLFLGSI VALKPETGEY VWHFQATPMD QWDYTSVQQI MTLDMPVKGE 370 380 390 400 410 420 MRHVIVHAPK NGFFYVLDAK TGEFLSGKNY VYQNWANGLD PLTGRPMYNP DGLYTLNGKF 430 440 450 460 470 480 WYGIPGPLGA HNFMAMAYSP KTHLVYIPAH QIPFGYKNQV GGFKPHADSW NVGLDMTKNG 490 500 510 520 530 540 LPDTPEARTA YIKDLHGWLL AWDPVKMETV WKIDHKGPWN GGILATGGDL LFQGLANGEF 550 560 570 580 590 600 HAYDATNGSD LYKFDAQSGI IAPPMTYSVN GKQYVAVEVG WGGIYPISMG GVGRTSGWTV 610 620 630 640 650 660 NHSYIAAFSL DGKAKLPALN NRGFLPVKPP AQYDQKVVDN GYFQYQTYCQ TCHGDNGEGA 670 680 690 700 710 720 GMLPDLRWAG AIRHQDAFYN VVGRGALTAY GMDRFDTSMT PDEIEAIRQY LIKRANDTYQ 730 740 REVDARKNDK NIPENPTLGI NP

References

[1]	"Cloning and sequencing of the gene encoding the 72-kilodalton dehydrogenase subunit of alcohol dehydrogenase from Acetobacter aceti." Inoue T., Sunagawa M., Mori A., Imai C., Fukuda M., Takagi M., Yano K. J. Bacteriol. 171:3115-3122(1989) [PubMed: 2722742] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-50.
[2]	"The structure of the quinoprotein alcohol dehydrogenase of Acetobacter aceti modelled on that of methanol dehydrogenase from Methylobacterium extorquens." Cozier G.E., Giles I.G., Anthony C. Biochem. J. 308:375-379(1995) [PubMed: 7772016] [Abstract] Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90004 Genomic DNA. Translation: BAA14058.1.
PIR	JS0326.
3D structure databases
HSSP	HSSP built from PDB template 1KB0 based on UniProtKB Q46444.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.99.8. 273.
Family and domain databases
InterPro	IPR009056. Cyt_c_monohaem. IPR019556. PQQ-dependent_C. IPR019551. PQQ-dependent_N. IPR018391. PQQ_beta_propeller_repeat. IPR017512. PQQ_MeOH/EtOH_DH. IPR002372. PQQ_repeat. IPR011047. Quino_AlcDH-like. IPR001479. Quinoprotein_DH_CS. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit. G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.
Pfam	PF01011. PQQ. 6 hits. PF10535. PQQ_C. 1 hit. PF10527. PQQ_N. 1 hit. [Graphical view]
SMART	SM00564. PQQ. 5 hits. [Graphical view]
TIGRFAMs	TIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITE	PS00363. BACTERIAL_PQQ_1. 1 hit. PS00364. BACTERIAL_PQQ_2. 1 hit. PS51007. CYTC. 1 hit. PS51318. TAT. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHET_ACEAC

Accession

Primary (citable) accession number: P18278

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)