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P18272

- NCAP_EBOZM

UniProt

P18272 - NCAP_EBOZM

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Protein

Nucleoprotein

Gene

NP

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral RNA genome packaging Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:NP
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000007209: Genome

Subcellular locationi

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell cytoplasm Source: UniProtKB-KW
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. viral nucleocapsid Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 739739NucleoproteinPRO_0000222171Add
BLAST

Post-translational modificationi

Phosphorylated and O-glycosylated by host.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homooligomer. Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with VP35 and VP30 to form the nucleocapsid. Also interacts with VP24 and VP40.2 Publications

Protein-protein interaction databases

IntActiP18272. 1 interaction.

Structurei

Secondary structure

1
739
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi646 – 65914Combined sources
Helixi661 – 67212Combined sources
Beta strandi676 – 6794Combined sources
Beta strandi685 – 6884Combined sources
Helixi690 – 6923Combined sources
Beta strandi693 – 6964Combined sources
Helixi702 – 7065Combined sources
Helixi708 – 7114Combined sources
Beta strandi712 – 7154Combined sources
Beta strandi718 – 7214Combined sources
Helixi722 – 7243Combined sources
Helixi727 – 73711Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QAZX-ray1.98A641-739[»]
4QB0X-ray1.75A641-739[»]
ProteinModelPortaliP18272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili334 – 36330Sequence AnalysisAdd
BLAST

Domaini

This protein can be divided into a hydrophobic N-terminal half, and a hydrophilic and highly acidic C-terminal half.

Sequence similaritiesi

Belongs to the filoviruses nucleoprotein family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR008609. Ebola_NP.
[Graphical view]
PfamiPF05505. Ebola_NP. 1 hit.
[Graphical view]
PIRSFiPIRSF003900. N_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

P18272-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSRPQKIWM APSLTESDMD YHKILTAGLS VQQGIVRQRV IPVYQVNNLE
60 70 80 90 100
EICQLIIQAF EAGVDFQESA DSFLLMLCLH HAYQGDYKLF LESGAVKYLE
110 120 130 140 150
GHGFRFEVKK RDGVKRLEEL LPAVSSGKNI KRTLAAMPEE ETTEANAGQF
160 170 180 190 200
LSFASLFLPK LVVGEKACLE KVQRQIQVHA EQGLIQYPTA WQSVGHMMVI
210 220 230 240 250
FRLMRTNFLI KFLLIHQGMH MVAGHDANDA VISNSVAQAR FSGLLIVKTV
260 270 280 290 300
LDHILQKTER GVRLHPLART AKVKNEVNSF KAALSSLAKH GEYAPFARLL
310 320 330 340 350
NLSGVNNLEH GLFPQLSAIA LGVATAHGST LAGVNVGEQY QQLREAATEA
360 370 380 390 400
EKQLQQYAES RELDHLGLDD QEKKILMNFH QKKNEISFQQ TNAMVTLRKE
410 420 430 440 450
RLAKLTEAIT AASLPKTSGH YDDDDDIPFP GPINDDDNPG HQDDDPTDSQ
460 470 480 490 500
DTTIPDVVVD PDDGSYGEYQ SYSENGMNAP DDLVLFDLDE DDEDTKPVPN
510 520 530 540 550
RSTKGGQQKN SQKGQHIEGR QTQSRPIQNV PGPHRTIHHA SAPLTDNDRR
560 570 580 590 600
NEPSGSTSPR MLTPINEEAD PLDDADDETS SLPPLESDDE EQDRDGTSNR
610 620 630 640 650
TPTVAPPAPV YRDHSEKKEL PQDEQQDQDH TQEARNQDSD NTQSEHSFEE
660 670 680 690 700
MYRHILRSQG PFDAVLYYHM MKDEPVVFST SDGKEYTYPD SLEEEYPPWL
710 720 730
TEKEAMNEEN RFVTLDGQQF YWPVMNHKNK FMAILQHHQ
Length:739
Mass (Da):83,287
Last modified:July 15, 1999 - v2
Checksum:i159C254EA0478886
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1701E → R in AAA42977. (PubMed:2718390)Curated
Sequence conflicti280 – 2801F → L in AAA42977. (PubMed:2718390)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721S → G in strain: Isolate mouse-adapted.
Natural varianti524 – 5241S → F in strain: Isolate guinea pig-adapted.
Natural varianti648 – 6481F → L in strain: Isolate guinea pig-adapted.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04337 Genomic RNA. Translation: AAA42977.1.
L11365 Genomic RNA. Translation: AAB81001.1.
AF086833 Genomic RNA. Translation: AAD14590.1.
AF272001 Genomic RNA. Translation: AAG40164.1.
AY142960 Genomic RNA. Translation: AAN37504.1.
AF499101 Genomic RNA. Translation: AAM76031.1.
PIRiA31471. VHIWEB.
RefSeqiNP_066243.1. NC_002549.1.

Genome annotation databases

GeneIDi911830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04337 Genomic RNA. Translation: AAA42977.1 .
L11365 Genomic RNA. Translation: AAB81001.1 .
AF086833 Genomic RNA. Translation: AAD14590.1 .
AF272001 Genomic RNA. Translation: AAG40164.1 .
AY142960 Genomic RNA. Translation: AAN37504.1 .
AF499101 Genomic RNA. Translation: AAM76031.1 .
PIRi A31471. VHIWEB.
RefSeqi NP_066243.1. NC_002549.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4QAZ X-ray 1.98 A 641-739 [» ]
4QB0 X-ray 1.75 A 641-739 [» ]
ProteinModelPortali P18272.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P18272. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911830.

Family and domain databases

InterProi IPR008609. Ebola_NP.
[Graphical view ]
Pfami PF05505. Ebola_NP. 1 hit.
[Graphical view ]
PIRSFi PIRSF003900. N_FiloV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The nucleoprotein gene of Ebola virus: cloning, sequencing, and in vitro expression."
    Sanchez A., Kiley M.P., Holloway B.P., McCormick J.B., Auperin D.D.
    Virology 170:81-91(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
    Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
    Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Molecular characterization of guinea pig-adapted variants of Ebola virus."
    Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
    Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate guinea pig-adapted.
  4. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate mouse-adapted.
  5. "The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
    Huang Y., Xu L., Sun Y., Nabel G.J.
    Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VP35; VP30 AND VP24.
  6. "Functional mapping of the nucleoprotein of Ebola virus."
    Watanabe S., Noda T., Kawaoka Y.
    J. Virol. 80:3743-3751(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
  7. "Mapping of the VP40-binding regions of the nucleoprotein of Ebola virus."
    Noda T., Watanabe S., Sagara H., Kawaoka Y.
    J. Virol. 81:3554-3562(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VP40.

Entry informationi

Entry nameiNCAP_EBOZM
AccessioniPrimary (citable) accession number: P18272
Secondary accession number(s): Q773N6
, Q8JS64, Q9DQD3, Q9YMG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 15, 1999
Last modified: November 26, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3