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Protein

Nucleoprotein

Gene

NP

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:NP
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Proteomesi
  • UP000007209 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • helical viral capsid Source: UniProtKB-KW
  • host cell cytoplasm Source: UniProtKB-SubCell
  • viral nucleocapsid Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002221711 – 739NucleoproteinAdd BLAST739

Post-translational modificationi

Phosphorylated and O-glycosylated by host.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homooligomer. Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with VP35 and VP30 to form the nucleocapsid. Also interacts with VP24 and VP40.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VP35Q051272EBI-9820219,EBI-6148294

Protein-protein interaction databases

IntActiP18272. 1 interactor.

Structurei

Secondary structure

1739
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 45Combined sources7
Helixi49 – 61Combined sources13
Helixi67 – 69Combined sources3
Helixi70 – 82Combined sources13
Helixi87 – 91Combined sources5
Helixi94 – 100Combined sources7
Turni101 – 103Combined sources3
Beta strandi104 – 110Combined sources7
Helixi117 – 120Combined sources4
Helixi128 – 135Combined sources8
Helixi147 – 155Combined sources9
Turni156 – 160Combined sources5
Helixi161 – 163Combined sources3
Helixi165 – 181Combined sources17
Helixi189 – 192Combined sources4
Helixi194 – 206Combined sources13
Helixi208 – 219Combined sources12
Helixi227 – 239Combined sources13
Turni240 – 243Combined sources4
Helixi245 – 253Combined sources9
Beta strandi255 – 257Combined sources3
Beta strandi262 – 264Combined sources3
Helixi266 – 268Combined sources3
Helixi271 – 273Combined sources3
Helixi274 – 288Combined sources15
Helixi289 – 296Combined sources8
Turni297 – 301Combined sources5
Helixi305 – 308Combined sources4
Helixi310 – 312Combined sources3
Helixi314 – 324Combined sources11
Turni326 – 328Combined sources3
Helixi336 – 349Combined sources14
Helixi646 – 659Combined sources14
Helixi661 – 672Combined sources12
Beta strandi676 – 679Combined sources4
Beta strandi685 – 688Combined sources4
Helixi690 – 692Combined sources3
Beta strandi693 – 696Combined sources4
Helixi702 – 706Combined sources5
Helixi708 – 711Combined sources4
Beta strandi712 – 715Combined sources4
Beta strandi718 – 721Combined sources4
Helixi722 – 724Combined sources3
Helixi727 – 737Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QAZX-ray1.98A641-739[»]
4QB0X-ray1.75A641-739[»]
4YPIX-ray3.71A/B/C/D38-385[»]
4Z9PX-ray1.79A36-351[»]
4ZTAX-ray2.40A33-367[»]
4ZTGX-ray2.80A33-367[»]
4ZTIX-ray2.40A/B33-367[»]
ProteinModelPortaliP18272.
SMRiP18272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili334 – 363Sequence analysisAdd BLAST30

Domaini

This protein can be divided into a hydrophobic N-terminal half, and a hydrophilic and highly acidic C-terminal half.

Sequence similaritiesi

Belongs to the filoviruses nucleoprotein family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR008609. Ebola_NP.
[Graphical view]
PfamiPF05505. Ebola_NP. 1 hit.
[Graphical view]
PIRSFiPIRSF003900. N_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

P18272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSRPQKIWM APSLTESDMD YHKILTAGLS VQQGIVRQRV IPVYQVNNLE
60 70 80 90 100
EICQLIIQAF EAGVDFQESA DSFLLMLCLH HAYQGDYKLF LESGAVKYLE
110 120 130 140 150
GHGFRFEVKK RDGVKRLEEL LPAVSSGKNI KRTLAAMPEE ETTEANAGQF
160 170 180 190 200
LSFASLFLPK LVVGEKACLE KVQRQIQVHA EQGLIQYPTA WQSVGHMMVI
210 220 230 240 250
FRLMRTNFLI KFLLIHQGMH MVAGHDANDA VISNSVAQAR FSGLLIVKTV
260 270 280 290 300
LDHILQKTER GVRLHPLART AKVKNEVNSF KAALSSLAKH GEYAPFARLL
310 320 330 340 350
NLSGVNNLEH GLFPQLSAIA LGVATAHGST LAGVNVGEQY QQLREAATEA
360 370 380 390 400
EKQLQQYAES RELDHLGLDD QEKKILMNFH QKKNEISFQQ TNAMVTLRKE
410 420 430 440 450
RLAKLTEAIT AASLPKTSGH YDDDDDIPFP GPINDDDNPG HQDDDPTDSQ
460 470 480 490 500
DTTIPDVVVD PDDGSYGEYQ SYSENGMNAP DDLVLFDLDE DDEDTKPVPN
510 520 530 540 550
RSTKGGQQKN SQKGQHIEGR QTQSRPIQNV PGPHRTIHHA SAPLTDNDRR
560 570 580 590 600
NEPSGSTSPR MLTPINEEAD PLDDADDETS SLPPLESDDE EQDRDGTSNR
610 620 630 640 650
TPTVAPPAPV YRDHSEKKEL PQDEQQDQDH TQEARNQDSD NTQSEHSFEE
660 670 680 690 700
MYRHILRSQG PFDAVLYYHM MKDEPVVFST SDGKEYTYPD SLEEEYPPWL
710 720 730
TEKEAMNEEN RFVTLDGQQF YWPVMNHKNK FMAILQHHQ
Length:739
Mass (Da):83,287
Last modified:July 15, 1999 - v2
Checksum:i159C254EA0478886
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170E → R in AAA42977 (PubMed:2718390).Curated1
Sequence conflicti280F → L in AAA42977 (PubMed:2718390).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti72S → G in strain: Isolate mouse-adapted. 1
Natural varianti524S → F in strain: Isolate guinea pig-adapted. 1
Natural varianti648F → L in strain: Isolate guinea pig-adapted. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04337 Genomic RNA. Translation: AAA42977.1.
L11365 Genomic RNA. Translation: AAB81001.1.
AF086833 Genomic RNA. Translation: AAD14590.1.
AF272001 Genomic RNA. Translation: AAG40164.1.
AY142960 Genomic RNA. Translation: AAN37504.1.
AF499101 Genomic RNA. Translation: AAM76031.1.
PIRiA31471. VHIWEB.
RefSeqiNP_066243.1. NC_002549.1.

Genome annotation databases

GeneIDi911830.
KEGGivg:911830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04337 Genomic RNA. Translation: AAA42977.1.
L11365 Genomic RNA. Translation: AAB81001.1.
AF086833 Genomic RNA. Translation: AAD14590.1.
AF272001 Genomic RNA. Translation: AAG40164.1.
AY142960 Genomic RNA. Translation: AAN37504.1.
AF499101 Genomic RNA. Translation: AAM76031.1.
PIRiA31471. VHIWEB.
RefSeqiNP_066243.1. NC_002549.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QAZX-ray1.98A641-739[»]
4QB0X-ray1.75A641-739[»]
4YPIX-ray3.71A/B/C/D38-385[»]
4Z9PX-ray1.79A36-351[»]
4ZTAX-ray2.40A33-367[»]
4ZTGX-ray2.80A33-367[»]
4ZTIX-ray2.40A/B33-367[»]
ProteinModelPortaliP18272.
SMRiP18272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP18272. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi911830.
KEGGivg:911830.

Family and domain databases

InterProiIPR008609. Ebola_NP.
[Graphical view]
PfamiPF05505. Ebola_NP. 1 hit.
[Graphical view]
PIRSFiPIRSF003900. N_FiloV. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCAP_EBOZM
AccessioniPrimary (citable) accession number: P18272
Secondary accession number(s): Q773N6
, Q8JS64, Q9DQD3, Q9YMG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 15, 1999
Last modified: November 30, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.