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P18272 (NCAP_EBOZM) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name=Protein N
Gene names
Name:NP
OrganismZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) [Reference proteome]
Taxonomic identifier128952 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length739 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.

Subunit structure

Homooligomer. Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with VP35 and VP30 to form the nucleocapsid. Also interacts with VP24 and VP40. Ref.5 Ref.7

Subcellular location

Virion. Host cytoplasm.

Domain

This protein can be divided into a hydrophobic N-terminal half, and a hydrophilic and highly acidic C-terminal half.

Post-translational modification

Phosphorylated and O-glycosylated by host.

Sequence similarities

Belongs to the filoviruses nucleoprotein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 739739Nucleoprotein
PRO_0000222171

Regions

Coiled coil334 – 36330 Potential

Natural variations

Natural variant721S → G in strain: Isolate mouse-adapted.
Natural variant5241S → F in strain: Isolate guinea pig-adapted.
Natural variant6481F → L in strain: Isolate guinea pig-adapted.

Experimental info

Sequence conflict1701E → R in AAA42977. Ref.1
Sequence conflict2801F → L in AAA42977. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P18272 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 159C254EA0478886

FASTA73983,287
        10         20         30         40         50         60 
MDSRPQKIWM APSLTESDMD YHKILTAGLS VQQGIVRQRV IPVYQVNNLE EICQLIIQAF 

        70         80         90        100        110        120 
EAGVDFQESA DSFLLMLCLH HAYQGDYKLF LESGAVKYLE GHGFRFEVKK RDGVKRLEEL 

       130        140        150        160        170        180 
LPAVSSGKNI KRTLAAMPEE ETTEANAGQF LSFASLFLPK LVVGEKACLE KVQRQIQVHA 

       190        200        210        220        230        240 
EQGLIQYPTA WQSVGHMMVI FRLMRTNFLI KFLLIHQGMH MVAGHDANDA VISNSVAQAR 

       250        260        270        280        290        300 
FSGLLIVKTV LDHILQKTER GVRLHPLART AKVKNEVNSF KAALSSLAKH GEYAPFARLL 

       310        320        330        340        350        360 
NLSGVNNLEH GLFPQLSAIA LGVATAHGST LAGVNVGEQY QQLREAATEA EKQLQQYAES 

       370        380        390        400        410        420 
RELDHLGLDD QEKKILMNFH QKKNEISFQQ TNAMVTLRKE RLAKLTEAIT AASLPKTSGH 

       430        440        450        460        470        480 
YDDDDDIPFP GPINDDDNPG HQDDDPTDSQ DTTIPDVVVD PDDGSYGEYQ SYSENGMNAP 

       490        500        510        520        530        540 
DDLVLFDLDE DDEDTKPVPN RSTKGGQQKN SQKGQHIEGR QTQSRPIQNV PGPHRTIHHA 

       550        560        570        580        590        600 
SAPLTDNDRR NEPSGSTSPR MLTPINEEAD PLDDADDETS SLPPLESDDE EQDRDGTSNR 

       610        620        630        640        650        660 
TPTVAPPAPV YRDHSEKKEL PQDEQQDQDH TQEARNQDSD NTQSEHSFEE MYRHILRSQG 

       670        680        690        700        710        720 
PFDAVLYYHM MKDEPVVFST SDGKEYTYPD SLEEEYPPWL TEKEAMNEEN RFVTLDGQQF 

       730 
YWPVMNHKNK FMAILQHHQ 

« Hide

References

[1]"The nucleoprotein gene of Ebola virus: cloning, sequencing, and in vitro expression."
Sanchez A., Kiley M.P., Holloway B.P., McCormick J.B., Auperin D.D.
Virology 170:81-91(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Molecular characterization of guinea pig-adapted variants of Ebola virus."
Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate guinea pig-adapted.
[4]Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate mouse-adapted.
[5]"The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
Huang Y., Xu L., Sun Y., Nabel G.J.
Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VP35; VP30 AND VP24.
[6]"Functional mapping of the nucleoprotein of Ebola virus."
Watanabe S., Noda T., Kawaoka Y.
J. Virol. 80:3743-3751(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION.
[7]"Mapping of the VP40-binding regions of the nucleoprotein of Ebola virus."
Noda T., Watanabe S., Sagara H., Kawaoka Y.
J. Virol. 81:3554-3562(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VP40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04337 Genomic RNA. Translation: AAA42977.1.
L11365 Genomic RNA. Translation: AAB81001.1.
AF086833 Genomic RNA. Translation: AAD14590.1.
AF272001 Genomic RNA. Translation: AAG40164.1.
AY142960 Genomic RNA. Translation: AAN37504.1.
AF499101 Genomic RNA. Translation: AAM76031.1.
PIRVHIWEB. A31471.
RefSeqNP_066243.1. NC_002549.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID911830.

Family and domain databases

InterProIPR008609. Ebola_NP.
[Graphical view]
PfamPF05505. Ebola_NP. 1 hit.
[Graphical view]
PIRSFPIRSF003900. N_FiloV. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNCAP_EBOZM
AccessionPrimary (citable) accession number: P18272
Secondary accession number(s): Q773N6 expand/collapse secondary AC list , Q8JS64, Q9DQD3, Q9YMG4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 15, 1999
Last modified: November 13, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families