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P18272

- NCAP_EBOZM

UniProt

P18272 - NCAP_EBOZM

Protein

Nucleoprotein

Gene

NP

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral RNA genome packaging Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    RNA-binding, Viral nucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoprotein
    Alternative name(s):
    Nucleocapsid protein
    Short name:
    Protein N
    Gene namesi
    Name:NP
    OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
    Taxonomic identifieri128952 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    ProteomesiUP000007209: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. helical viral capsid Source: UniProtKB-KW
    2. host cell cytoplasm Source: UniProtKB-SubCell
    3. ribonucleoprotein complex Source: UniProtKB-KW
    4. viral nucleocapsid Source: CACAO

    Keywords - Cellular componenti

    Capsid protein, Helical capsid protein, Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 739739NucleoproteinPRO_0000222171Add
    BLAST

    Post-translational modificationi

    Phosphorylated and O-glycosylated by host.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homooligomer. Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with VP35 and VP30 to form the nucleocapsid. Also interacts with VP24 and VP40.2 Publications

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili334 – 36330Sequence AnalysisAdd
    BLAST

    Domaini

    This protein can be divided into a hydrophobic N-terminal half, and a hydrophilic and highly acidic C-terminal half.

    Sequence similaritiesi

    Belongs to the filoviruses nucleoprotein family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR008609. Ebola_NP.
    [Graphical view]
    PfamiPF05505. Ebola_NP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003900. N_FiloV. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P18272-1 [UniParc]FASTAAdd to Basket

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    MDSRPQKIWM APSLTESDMD YHKILTAGLS VQQGIVRQRV IPVYQVNNLE    50
    EICQLIIQAF EAGVDFQESA DSFLLMLCLH HAYQGDYKLF LESGAVKYLE 100
    GHGFRFEVKK RDGVKRLEEL LPAVSSGKNI KRTLAAMPEE ETTEANAGQF 150
    LSFASLFLPK LVVGEKACLE KVQRQIQVHA EQGLIQYPTA WQSVGHMMVI 200
    FRLMRTNFLI KFLLIHQGMH MVAGHDANDA VISNSVAQAR FSGLLIVKTV 250
    LDHILQKTER GVRLHPLART AKVKNEVNSF KAALSSLAKH GEYAPFARLL 300
    NLSGVNNLEH GLFPQLSAIA LGVATAHGST LAGVNVGEQY QQLREAATEA 350
    EKQLQQYAES RELDHLGLDD QEKKILMNFH QKKNEISFQQ TNAMVTLRKE 400
    RLAKLTEAIT AASLPKTSGH YDDDDDIPFP GPINDDDNPG HQDDDPTDSQ 450
    DTTIPDVVVD PDDGSYGEYQ SYSENGMNAP DDLVLFDLDE DDEDTKPVPN 500
    RSTKGGQQKN SQKGQHIEGR QTQSRPIQNV PGPHRTIHHA SAPLTDNDRR 550
    NEPSGSTSPR MLTPINEEAD PLDDADDETS SLPPLESDDE EQDRDGTSNR 600
    TPTVAPPAPV YRDHSEKKEL PQDEQQDQDH TQEARNQDSD NTQSEHSFEE 650
    MYRHILRSQG PFDAVLYYHM MKDEPVVFST SDGKEYTYPD SLEEEYPPWL 700
    TEKEAMNEEN RFVTLDGQQF YWPVMNHKNK FMAILQHHQ 739
    Length:739
    Mass (Da):83,287
    Last modified:July 15, 1999 - v2
    Checksum:i159C254EA0478886
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti170 – 1701E → R in AAA42977. (PubMed:2718390)Curated
    Sequence conflicti280 – 2801F → L in AAA42977. (PubMed:2718390)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti72 – 721S → G in strain: Isolate mouse-adapted.
    Natural varianti524 – 5241S → F in strain: Isolate guinea pig-adapted.
    Natural varianti648 – 6481F → L in strain: Isolate guinea pig-adapted.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04337 Genomic RNA. Translation: AAA42977.1.
    L11365 Genomic RNA. Translation: AAB81001.1.
    AF086833 Genomic RNA. Translation: AAD14590.1.
    AF272001 Genomic RNA. Translation: AAG40164.1.
    AY142960 Genomic RNA. Translation: AAN37504.1.
    AF499101 Genomic RNA. Translation: AAM76031.1.
    PIRiA31471. VHIWEB.
    RefSeqiNP_066243.1. NC_002549.1.

    Genome annotation databases

    GeneIDi911830.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04337 Genomic RNA. Translation: AAA42977.1 .
    L11365 Genomic RNA. Translation: AAB81001.1 .
    AF086833 Genomic RNA. Translation: AAD14590.1 .
    AF272001 Genomic RNA. Translation: AAG40164.1 .
    AY142960 Genomic RNA. Translation: AAN37504.1 .
    AF499101 Genomic RNA. Translation: AAM76031.1 .
    PIRi A31471. VHIWEB.
    RefSeqi NP_066243.1. NC_002549.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 911830.

    Family and domain databases

    InterProi IPR008609. Ebola_NP.
    [Graphical view ]
    Pfami PF05505. Ebola_NP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003900. N_FiloV. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleoprotein gene of Ebola virus: cloning, sequencing, and in vitro expression."
      Sanchez A., Kiley M.P., Holloway B.P., McCormick J.B., Auperin D.D.
      Virology 170:81-91(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
      Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
      Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Molecular characterization of guinea pig-adapted variants of Ebola virus."
      Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
      Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate guinea pig-adapted.
    4. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate mouse-adapted.
    5. "The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
      Huang Y., Xu L., Sun Y., Nabel G.J.
      Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VP35; VP30 AND VP24.
    6. "Functional mapping of the nucleoprotein of Ebola virus."
      Watanabe S., Noda T., Kawaoka Y.
      J. Virol. 80:3743-3751(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.
    7. "Mapping of the VP40-binding regions of the nucleoprotein of Ebola virus."
      Noda T., Watanabe S., Sagara H., Kawaoka Y.
      J. Virol. 81:3554-3562(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VP40.

    Entry informationi

    Entry nameiNCAP_EBOZM
    AccessioniPrimary (citable) accession number: P18272
    Secondary accession number(s): Q773N6
    , Q8JS64, Q9DQD3, Q9YMG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3