Reviewed, UniProtKB/Swiss-Prot P18266 (GSK3B_RAT)

Names and origin

Protein names

Recommended name:
    Glycogen synthase kinase-3 beta
      Short name=GSK-3 beta
    EC=2.7.11.26
Alternative name(s):
    Factor A
      Short name=FA

Gene names

Name:

Gsk3b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin By similarity.
Catalytic activity	ATP + [tau protein] = ADP + [tau protein] phosphate.
Subunit structure	Monomer. Interacts with AXIN1, CABYR, MUC1, NIN and PRUNE By similarity.
Post-translational modification	Phosphorylation of Tyr-216 is necessary for the activity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW establishment of cell polarity Inferred from direct assay. Source: RGD induction of apoptosis Inferred from direct assay. Source: RGD negative regulation of MAP kinase activity Inferred from mutant phenotype. Source: RGD negative regulation of dendrite morphogenesis Inferred from mutant phenotype. Source: RGD negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: RGD protein amino acid phosphorylation Inferred from direct assay. Source: UniProtKB regulation of neuronal synaptic plasticity Inferred from mutant phenotype. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD dendritic spine Inferred from direct assay. Source: RGD membrane raft Inferred from direct assay. Source: RGD protein complex Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from direct assay. Source: RGD glycogen synthase kinase 3 activity Ref.1 Inferred from direct assay. Source: UniProtKB ionotropic glutamate receptor binding Inferred from physical interaction. Source: RGD protein kinase binding Inferred from physical interaction. Source: RGD tau protein binding Inferred from direct assay. Source: RGD tau-protein kinase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Glycogen synthase kinase-3 beta		PRO_0000085982
Regions
	Domain	56 – 340	285	Protein kinase
	Nucleotide binding	62 – 70	9	ATP By similarity
Sites
	Active site	181	1	Proton acceptor By similarity
	Binding site	85	1	ATP By similarity
Amino acid modifications
	Modified residue	7	1	Phosphothreonine By similarity
	Modified residue	9	1	Phosphoserine; by PKB/AKT1 By similarity
	Modified residue	215	1	Phosphoserine By similarity
	Modified residue	216	1	Phosphotyrosine Ref.3
	Modified residue	219	1	Phosphoserine By similarity
	Modified residue	275	1	Phosphothreonine By similarity
	Modified residue	277	1	Phosphothreonine By similarity
	Modified residue	389	1	Phosphoserine By similarity
	Modified residue	395	1	Phosphothreonine By similarity
Experimental info
	Mutagenesis	216	1	Y → F: Loss of phosphorylation.
	Sequence conflict	240	1	M → V in CAA52020. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18266-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 2F473FCAB89B4398	FASTA	420	46,742
	10 20 30 40 50 60 MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK 70 80 90 100 110 120 VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG 130 140 150 160 170 180 EKKDEVYLNL VLDYVPETVY RVARHYSRAK QTLPVIYVKL YMYQLFRSLA YIHSFGICHR 190 200 210 220 230 240 DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDM 250 260 270 280 290 300 WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP 310 320 330 340 350 360 WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL PNGRDTPALF 370 380 390 400 410 420 NFTTQELSSN PPLATILIPP HARIQAAASP PANATAASDT NAGDRGQTNN AASASASNST

References

[1]	"Molecular cloning and expression of glycogen synthase kinase-3/factor A." Woodgett J.R. EMBO J. 9:2431-2438(1990) [PubMed: 2164470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"Glycogen synthase kinase 3 beta is identical to tau protein kinase I generating several epitopes of paired helical filaments." Ishiguro K., Shiratsuchi A., Sato S., Omori A., Arioka M., Kobayashi S., Uchida T., Imahori K. FEBS Lett. 325:167-172(1993) [PubMed: 7686508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain cortex.
[3]	"Modulation of the glycogen synthase kinase-3 family by tyrosine phosphorylation." Hughes K., Nikolakaki E., Plyte S.E., Totty N.F., Woodgett J.R. EMBO J. 12:803-808(1993) [PubMed: 8382613] [Abstract] Cited for: PHOSPHORYLATION AT TYR-216.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53428 mRNA. Translation: CAA37519.1. X73653 mRNA. Translation: CAA52020.1.
IPI	IPI00324168.
PIR	TVRTKB. S14708.
RefSeq	NP_114469.1.
UniGene	Rn.10426
3D structure databases
HSSP	HSSP built from PDB template 1I09 based on UniProtKB P49841.
SMR	P18266. Positions 23-386.
ModBase	Search...
Protein-protein interaction databases
STRING	P18266.
PTM databases
PhosphoSite	P18266.
Genome annotation databases
Ensembl	ENSRNOT00000003867; ENSRNOP00000003867; ENSRNOG00000002833; Rattus norvegicus. [Genome view]
GeneID	84027.
KEGG	rno:84027.
UCSC	NM_032080. rat.
Organism-specific databases
CTD	84027.
RGD	70982. Gsk3b.
Phylogenomic databases
HOVERGEN	P18266.
Enzyme and pathway databases
BRENDA	2.7.11.26. 248.
Gene expression databases
ArrayExpress	P18266.
Genevestigator	P18266.
GermOnline	ENSRNOG00000002833. Rattus norvegicus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	616603.

Entry information

Entry name

GSK3B_RAT

Accession

Primary (citable) accession number: P18266

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	November 3, 2009
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)