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P18251 (BLA1_ENTCL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase Ohio-1

EC=3.5.2.6
Encoded onPlasmid pDS075
OrganismEnterobacter cloacae
Taxonomic identifier550 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 286265Beta-lactamase Ohio-1
PRO_0000016996

Regions

Region230 – 2323Substrate binding By similarity

Sites

Active site661Acyl-ester intermediate By similarity
Active site1641Proton acceptor By similarity

Amino acid modifications

Disulfide bond73 ↔ 119 By similarity

Sequences

Sequence LengthMass (Da)Tools
P18251 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 9FE16709361AF9C5

FASTA28631,354
        10         20         30         40         50         60 
MRYFRLCIIS LLATLPLRVH AGPQPLEQIK LSESQLSGSV GMIEMDLARP GTLTAWRADE 

        70         80         90        100        110        120 
RFPMMSTFKV VLCGAGLARV DAGDEQLERK IHYRRQDLVD YSPVSEKHLA DGMTVGELCA 

       130        140        150        160        170        180 
AAITMSDNSA ANLLLPAVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTAR 

       190        200        210        220        230        240 
SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGRLIRSV LPAGWFIADK TGAGERGARG 

       250        260        270        280 
IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIAGAL IEHWQR 

« Hide

References

[1]"OHIO-1 beta-lactamase is part of the SHV-1 family."
Shlaes D.M., Currie-Mccumber C., Hull A., Behlau I., Kron M.
Antimicrob. Agents Chemother. 34:1570-1576(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33655 Unassigned DNA. Translation: AAA72078.1.
PIRA44958.

3D structure databases

ProteinModelPortalP18251.
SMRP18251. Positions 23-286.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP18251.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLA1_ENTCL
AccessionPrimary (citable) accession number: P18251
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families