P18251 (BLA1_ENTCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-lactamase Ohio-1 EC=3.5.2.6
Encoded on	Plasmid pDS075
Organism	Enterobacter cloacae
Taxonomic identifier	550 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 286	265	Beta-lactamase Ohio-1		PRO_0000016996
Regions
	Region	230 – 232	3	Substrate binding By similarity
Sites
	Active site	66	1	Acyl-ester intermediate By similarity
	Active site	164	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 119		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18251 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 9FE16709361AF9C5	FASTA	286	31,354
	10 20 30 40 50 60 MRYFRLCIIS LLATLPLRVH AGPQPLEQIK LSESQLSGSV GMIEMDLARP GTLTAWRADE 70 80 90 100 110 120 RFPMMSTFKV VLCGAGLARV DAGDEQLERK IHYRRQDLVD YSPVSEKHLA DGMTVGELCA 130 140 150 160 170 180 AAITMSDNSA ANLLLPAVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTAR 190 200 210 220 230 240 SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGRLIRSV LPAGWFIADK TGAGERGARG 250 260 270 280 IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIAGAL IEHWQR

References

[1]	"OHIO-1 beta-lactamase is part of the SHV-1 family." Shlaes D.M., Currie-Mccumber C., Hull A., Behlau I., Kron M. Antimicrob. Agents Chemother. 34:1570-1576(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33655 Unassigned DNA. Translation: AAA72078.1.
PIR	A44958.
3D structure databases
ProteinModelPortal	P18251.
SMR	P18251. Positions 23-286.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P18251.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA1_ENTCL

Accession

Primary (citable) accession number: P18251

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program