P18242 (CATD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cathepsin D
EC=3.4.23.5

Gene names

Name:

Ctsd

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acid protease active in intracellular protein breakdown.
Catalytic activity	Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.
Subunit structure	Consists of a light chain and a heavy chain By similarity.
Subcellular location	Lysosome. Melanosome By similarity. Secreted › extracellular space By similarity.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from mutant phenotype PubMed 15452145. Source: MGI proteolysis Inferred by curator PubMed 15588329. Source: MGI
Cellular_component	extracellular matrix Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from direct assay PubMed 10591619PubMed 12775715PubMed 15452145PubMed 16242638PubMed 18337546PubMed 9034150PubMed 9645704. Source: MGI melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 14651853. Source: MGI
Molecular_function	aspartic-type endopeptidase activity Inferred from direct assay PubMed 19293336. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 64	44	Activation peptide Potential		PRO_0000025953
	Chain	65 – 410	346	Cathepsin D		PRO_0000025954
Sites
	Active site	97	1	By similarity
	Active site	293	1	By similarity
Amino acid modifications
	Glycosylation	134	1	N-linked (GlcNAc...) By similarity
	Glycosylation	261	1	N-linked (GlcNAc...) (high mannose) Ref.5
	Disulfide bond	91 ↔ 160		By similarity
	Disulfide bond	110 ↔ 117		By similarity
	Disulfide bond	284 ↔ 288		By similarity
	Disulfide bond	327 ↔ 364		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18242 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: DC4928EC46928BF0	FASTA	410	44,954
	10 20 30 40 50 60 MKTPGVLLLI LGLLASSSFA IIRIPLRKFT SIRRTMTEVG GSVEDLILKG PITKYSMQSS 70 80 90 100 110 120 PKTTEPVSEL LKNYLDAQYY GDIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KILDIACWVH 130 140 150 160 170 180 HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC KSDQSKARGI KVEKQIFGEA 190 200 210 220 230 240 TKQPGIVFVA AKFDGILGMG YPHISVNNVL PVFDNLMQQK LVDKNIFSFY LNRDPEGQPG 250 260 270 280 290 300 GELMLGGTDS KYYHGELSYL NVTRKAYWQV HMDQLEVGNE LTLCKGGCEA IVDTGTSLLV 310 320 330 340 350 360 GPVEEVKELQ KAIGAVPLIQ GEYMIPCEKV SSLPTVYLKL GGKNYELHPD KYILKVSQGG 370 380 390 400 410 KTICLSGFMG MDIPPPSGPL WILGDVFIGS YYTVFDRDNN RVGFANAVVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cDNA encoding mouse cathepsin D." Diedrich J.F., Staskus K.A., Retzel E.F., Haase A.T. Nucleic Acids Res. 18:7184-7184(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Brain.
[2]	"Molecular cloning of mouse cathepsin D." Grusby M.J., Mitchell S.C., Glimcher L.H. Nucleic Acids Res. 18:4008-4008(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Mouse cathepsin D gene: molecular organization, characterization of the promoter, and chromosomal localization." Hetman M., Perschl A., Saftig P., von Figura K., Peters C. DNA Cell Biol. 13:419-427(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Brain and Mammary gland.
[5]	"High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis." Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S. Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-261, MASS SPECTROMETRY. Tissue: Epidermis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53337 mRNA. Translation: CAA37423.1. X52886 mRNA. Translation: CAA37067.1. X68378 , X68379, X68380, X68381, X68382, X68383 Genomic DNA. Translation: CAA48453.1. BC054758 mRNA. Translation: AAH54758.1. BC057931 mRNA. Translation: AAH57931.1.
IPI	IPI00111013.
PIR	KHMSD. I48278.
RefSeq	NP_034113.1. NM_009983.2.
UniGene	Mm.231395.
3D structure databases
ProteinModelPortal	P18242.
SMR	P18242. Positions 21-408.
ModBase	Search...
Protein-protein interaction databases
IntAct	P18242. 2 interactions.
Protein family/group databases
MEROPS	A01.009.
PTM databases
PhosphoSite	P18242.
Proteomic databases
PaxDb	P18242.
PRIDE	P18242.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000151120; ENSMUSP00000121203; ENSMUSG00000007891.
GeneID	13033.
KEGG	mmu:13033.
Organism-specific databases
CTD	1509.
MGI	MGI:88562. Ctsd.
Phylogenomic databases
eggNOG	NOG248684.
HOGENOM	HOG000197681.
HOVERGEN	HBG000482.
KO	K01379.
OMA	NIACLMH.
Gene expression databases
ArrayExpress	P18242.
Bgee	P18242.
CleanEx	MM_CTSD.
Genevestigator	P18242.
GermOnline	ENSMUSG00000007891. Mus musculus.
Family and domain databases
Gene3D	2.40.70.10. 2 hits.
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR012848. Propep_A1. [Graphical view]
PANTHER	PTHR13683. PTHR13683. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CTSD. mouse.
NextBio	282908.
SOURCE	Search...

Entry information

Entry name

CATD_MOUSE

Accession

Primary (citable) accession number: P18242

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program