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Protein

Cathepsin D

Gene

Ctsd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acid protease active in intracellular protein breakdown.

Catalytic activityi

Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei97 – 971PROSITE-ProRule annotation
Active sitei293 – 2931PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: MGI
  2. peptidase activity Source: MGI

GO - Biological processi

  1. autophagic vacuole assembly Source: MGI
  2. proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.23.5. 3474.
ReactomeiREACT_313067. Collagen degradation.
REACT_316383. MHC class II antigen presentation.

Protein family/group databases

MEROPSiA01.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin D (EC:3.4.23.5)
Gene namesi
Name:Ctsd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:88562. Ctsd.

Subcellular locationi

  1. Lysosome
  2. Melanosome By similarity
  3. Secretedextracellular space By similarity

GO - Cellular componenti

  1. extracellular space Source: MGI
  2. extracellular vesicular exosome Source: MGI
  3. lysosome Source: MGI
  4. melanosome Source: UniProtKB-SubCell
  5. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Propeptidei21 – 6444Activation peptideSequence AnalysisPRO_0000025953Add
BLAST
Chaini65 – 410346Cathepsin DPRO_0000025954Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi91 ↔ 160By similarity
Disulfide bondi110 ↔ 117By similarity
Glycosylationi134 – 1341N-linked (GlcNAc...)By similarity
Glycosylationi261 – 2611N-linked (GlcNAc...) (high mannose)1 Publication
Disulfide bondi284 ↔ 288By similarity
Disulfide bondi327 ↔ 364By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP18242.
PaxDbiP18242.
PRIDEiP18242.

PTM databases

PhosphoSiteiP18242.

Expressioni

Gene expression databases

BgeeiP18242.
CleanExiMM_CTSD.
ExpressionAtlasiP18242. baseline and differential.
GenevestigatoriP18242.

Interactioni

Subunit structurei

Consists of a light chain and a heavy chain.By similarity

Protein-protein interaction databases

IntActiP18242. 10 interactions.
MINTiMINT-4089919.

Structurei

3D structure databases

ProteinModelPortaliP18242.
SMRiP18242. Positions 21-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248684.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP18242.
KOiK01379.
OMAiTEGPIPE.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTPGVLLLI LGLLASSSFA IIRIPLRKFT SIRRTMTEVG GSVEDLILKG
60 70 80 90 100
PITKYSMQSS PKTTEPVSEL LKNYLDAQYY GDIGIGTPPQ CFTVVFDTGS
110 120 130 140 150
SNLWVPSIHC KILDIACWVH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY
160 170 180 190 200
LSQDTVSVPC KSDQSKARGI KVEKQIFGEA TKQPGIVFVA AKFDGILGMG
210 220 230 240 250
YPHISVNNVL PVFDNLMQQK LVDKNIFSFY LNRDPEGQPG GELMLGGTDS
260 270 280 290 300
KYYHGELSYL NVTRKAYWQV HMDQLEVGNE LTLCKGGCEA IVDTGTSLLV
310 320 330 340 350
GPVEEVKELQ KAIGAVPLIQ GEYMIPCEKV SSLPTVYLKL GGKNYELHPD
360 370 380 390 400
KYILKVSQGG KTICLSGFMG MDIPPPSGPL WILGDVFIGS YYTVFDRDNN
410
RVGFANAVVL
Length:410
Mass (Da):44,954
Last modified:November 1, 1990 - v1
Checksum:iDC4928EC46928BF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53337 mRNA. Translation: CAA37423.1.
X52886 mRNA. Translation: CAA37067.1.
X68378
, X68379, X68380, X68381, X68382, X68383 Genomic DNA. Translation: CAA48453.1.
BC054758 mRNA. Translation: AAH54758.1.
BC057931 mRNA. Translation: AAH57931.1.
CCDSiCCDS22029.1.
PIRiI48278. KHMSD.
RefSeqiNP_034113.1. NM_009983.2.
UniGeneiMm.231395.

Genome annotation databases

EnsembliENSMUST00000151120; ENSMUSP00000121203; ENSMUSG00000007891.
GeneIDi13033.
KEGGimmu:13033.
UCSCiuc009kmv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53337 mRNA. Translation: CAA37423.1.
X52886 mRNA. Translation: CAA37067.1.
X68378
, X68379, X68380, X68381, X68382, X68383 Genomic DNA. Translation: CAA48453.1.
BC054758 mRNA. Translation: AAH54758.1.
BC057931 mRNA. Translation: AAH57931.1.
CCDSiCCDS22029.1.
PIRiI48278. KHMSD.
RefSeqiNP_034113.1. NM_009983.2.
UniGeneiMm.231395.

3D structure databases

ProteinModelPortaliP18242.
SMRiP18242. Positions 21-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP18242. 10 interactions.
MINTiMINT-4089919.

Protein family/group databases

MEROPSiA01.009.

PTM databases

PhosphoSiteiP18242.

Proteomic databases

MaxQBiP18242.
PaxDbiP18242.
PRIDEiP18242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000151120; ENSMUSP00000121203; ENSMUSG00000007891.
GeneIDi13033.
KEGGimmu:13033.
UCSCiuc009kmv.1. mouse.

Organism-specific databases

CTDi1509.
MGIiMGI:88562. Ctsd.

Phylogenomic databases

eggNOGiNOG248684.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP18242.
KOiK01379.
OMAiTEGPIPE.
TreeFamiTF314990.

Enzyme and pathway databases

BRENDAi3.4.23.5. 3474.
ReactomeiREACT_313067. Collagen degradation.
REACT_316383. MHC class II antigen presentation.

Miscellaneous databases

ChiTaRSiCtsd. mouse.
NextBioi282908.
PROiP18242.
SOURCEiSearch...

Gene expression databases

BgeeiP18242.
CleanExiMM_CTSD.
ExpressionAtlasiP18242. baseline and differential.
GenevestigatoriP18242.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a cDNA encoding mouse cathepsin D."
    Diedrich J.F., Staskus K.A., Retzel E.F., Haase A.T.
    Nucleic Acids Res. 18:7184-7184(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Mouse cathepsin D gene: molecular organization, characterization of the promoter, and chromosomal localization."
    Hetman M., Perschl A., Saftig P., von Figura K., Peters C.
    DNA Cell Biol. 13:419-427(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Mammary gland.
  5. "High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis."
    Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S.
    Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-261.
    Tissue: Epidermis.

Entry informationi

Entry nameiCATD_MOUSE
AccessioniPrimary (citable) accession number: P18242
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 1, 2015
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.