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Protein

Cathepsin D

Gene

Ctsd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation.By similarity

Catalytic activityi

Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei97PROSITE-ProRule annotation1
Active sitei293PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: MGI
  • hydrolase activity Source: MGI
  • peptidase activity Source: MGI

GO - Biological processi

  • autophagosome assembly Source: MGI
  • protein catabolic process Source: GO_Central
  • proteolysis Source: MGI

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.23.5. 3474.
ReactomeiR-MMU-1442490. Collagen degradation.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiA01.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin D (EC:3.4.23.5)
Gene namesi
Name:Ctsd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:88562. Ctsd.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • lysosome Source: MGI
  • melanosome Source: UniProtKB-SubCell
  • membrane raft Source: MGI
  • mitochondrion Source: MGI

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002595321 – 64Activation peptideSequence analysisAdd BLAST44
ChainiPRO_000002595465 – 410Cathepsin DAdd BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi91 ↔ 160By similarity
Disulfide bondi110 ↔ 117By similarity
Glycosylationi134N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi261N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Disulfide bondi284 ↔ 288By similarity
Disulfide bondi327 ↔ 364By similarity

Post-translational modificationi

N- and O-glycosylated.By similarity1 Publication
Undergoes proteolytic cleavage and activation by ADAM30.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP18242.
PaxDbiP18242.
PeptideAtlasiP18242.
PRIDEiP18242.

PTM databases

iPTMnetiP18242.
PhosphoSitePlusiP18242.
UniCarbKBiP18242.

Expressioni

Gene expression databases

BgeeiENSMUSG00000007891.
CleanExiMM_CTSD.
ExpressionAtlasiP18242. baseline and differential.
GenevisibleiP18242. MM.

Interactioni

Subunit structurei

Consists of a light chain and a heavy chain. Interacts with ADAM30; this leads to activation of CTSD.By similarity

Protein-protein interaction databases

BioGridi198970. 3 interactors.
IntActiP18242. 10 interactors.
MINTiMINT-4089919.
STRINGi10090.ENSMUSP00000121203.

Structurei

3D structure databases

ProteinModelPortaliP18242.
SMRiP18242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 405Peptidase A1PROSITE-ProRule annotationAdd BLAST327

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP18242.
KOiK01379.
OMAiWNGQYTV.
OrthoDBiEOG091G0JP7.
TreeFamiTF314990.

Family and domain databases

CDDicd05490. Cathepsin_D2. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033736. Cathepsin_chordata.
IPR033144. Cathepsin_D.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF487. PTHR13683:SF487. 1 hit.
PfamiView protein in Pfam
PF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTPGVLLLI LGLLASSSFA IIRIPLRKFT SIRRTMTEVG GSVEDLILKG
60 70 80 90 100
PITKYSMQSS PKTTEPVSEL LKNYLDAQYY GDIGIGTPPQ CFTVVFDTGS
110 120 130 140 150
SNLWVPSIHC KILDIACWVH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY
160 170 180 190 200
LSQDTVSVPC KSDQSKARGI KVEKQIFGEA TKQPGIVFVA AKFDGILGMG
210 220 230 240 250
YPHISVNNVL PVFDNLMQQK LVDKNIFSFY LNRDPEGQPG GELMLGGTDS
260 270 280 290 300
KYYHGELSYL NVTRKAYWQV HMDQLEVGNE LTLCKGGCEA IVDTGTSLLV
310 320 330 340 350
GPVEEVKELQ KAIGAVPLIQ GEYMIPCEKV SSLPTVYLKL GGKNYELHPD
360 370 380 390 400
KYILKVSQGG KTICLSGFMG MDIPPPSGPL WILGDVFIGS YYTVFDRDNN
410
RVGFANAVVL
Length:410
Mass (Da):44,954
Last modified:November 1, 1990 - v1
Checksum:iDC4928EC46928BF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53337 mRNA. Translation: CAA37423.1.
X52886 mRNA. Translation: CAA37067.1.
X68378
, X68379, X68380, X68381, X68382, X68383 Genomic DNA. Translation: CAA48453.1.
BC054758 mRNA. Translation: AAH54758.1.
BC057931 mRNA. Translation: AAH57931.1.
CCDSiCCDS22029.1.
PIRiI48278. KHMSD.
RefSeqiNP_034113.1. NM_009983.2.
UniGeneiMm.231395.

Genome annotation databases

EnsembliENSMUST00000151120; ENSMUSP00000121203; ENSMUSG00000007891.
GeneIDi13033.
KEGGimmu:13033.
UCSCiuc009kmv.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCATD_MOUSE
AccessioniPrimary (citable) accession number: P18242
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 7, 2017
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families