Reviewed, UniProtKB/Swiss-Prot P18239 (ADT2_YEAST)

Names and origin

Protein names

Recommended name:
    ADP,ATP carrier protein 2
Alternative name(s):
    ADP/ATP translocase 2
    Adenine nucleotide translocator 2
      Short name=ANT 2

Gene names

Name:	AAC2
Synonyms:	PET9
Ordered Locus Names:	YBL030C
ORF Names:	YBL0421

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Miscellaneous	The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. At least 2 of the odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue By similarity.
Sequence similarities	Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ADP transport Inferred from direct assay. Source: SGD ATP transport Inferred from direct assay. Source: SGD aerobic respiration Inferred from mutant phenotype. Source: SGD anaerobic respiration Inferred from genetic interaction. Source: SGD apoptosis Inferred from mutant phenotype. Source: SGD heme biosynthetic process Inferred from genetic interaction. Source: SGD mitochondrial transport Inferred from mutant phenotype. Source: SGD transmembrane transport Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from direct assay. Source: SGD
Molecular function	ATP:ADP antiporter activity Inferred from direct assay. Source: SGD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	ADP,ATP carrier protein 2		PRO_0000090594
Regions
	Transmembrane	20 – 54	35	1 By similarity
	Transmembrane	91 – 115	25	2 By similarity
	Transmembrane	123 – 157	35	3 By similarity
	Transmembrane	192 – 219	28	4 By similarity
	Transmembrane	224 – 258	35	5 By similarity
	Transmembrane	287 – 312	26	6 By similarity
	Repeat	21 – 114	94	Solcar 1
	Repeat	125 – 217	93	Solcar 2
	Repeat	225 – 311	87	Solcar 3
	Motif	252 – 257	6	Substrate recognition By similarity
Sites
	Binding site	96	1	Nucleotide By similarity
Amino acid modifications
	Modified residue	39	1	Phosphothreonine Ref.8
	Modified residue	42	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	58	1	L → I in AAA97484. Ref.2
	Sequence conflict	65	1	R → K in AAA97484. Ref.2
	Sequence conflict	68	1	A → S in AAA97484. Ref.2
	Sequence conflict	71	1	L → V in AAA97484. Ref.2
	Sequence conflict	79	1	T → K in AAA97484. Ref.2
	Sequence conflict	83	1	V → L in AAA97484. Ref.2
	Sequence conflict	113	1	A → L in AAA97484. Ref.2
	Sequence conflict	124	1	A → G in AAA97484. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18239-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: A9805DC33D9E24AC	FASTA	318	34,426
	10 20 30 40 50 60 MSSNAQVKTP LPPAPAPKKE SNFLIDFLMG GVSAAVAKTA ASPIERVKLL IQNQDEMLKQ 70 80 90 100 110 120 GTLDRKYAGI LDCFKRTATQ EGVISFWRGN TANVIRYFPT QALNFAFKDK IKAMFGFKKE 130 140 150 160 170 180 EGYAKWFAGN LASGGAAGAL SLLFVYSLDY ARTRLAADSK SSKKGGARQF NGLIDVYKKT 190 200 210 220 230 240 LKSDGVAGLY RGFLPSVVGI VVYRGLYFGM YDSLKPLLLT GSLEGSFLAS FLLGWVVTTG 250 260 270 280 290 300 ASTCSYPLDT VRRRMMMTSG QAVKYDGAFD CLRKIVAAEG VGSLFKGCGA NILRGVAGAG 310 VISMYDQLQM ILFGKKFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Separate genes encode functionally equivalent ADP/ATP carrier proteins in Saccharomyces cerevisiae. Isolation and analysis of AAC2." Lawson J.E., Douglas M.G. J. Biol. Chem. 263:14812-14818(1988) [PubMed: 2844786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A third ADP/ATP translocator gene in yeast." Kolarov J., Kolarova N., Nelson N. J. Biol. Chem. 265:12711-12716(1990) [PubMed: 2165073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Trezeguet V., le Saux A., Lauquin G. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen." van Dyck L., Jonniaux J.-L., Barreiros T.D.M., Kleine K., Goffeau A. Yeast 10:1663-1673(1994) [PubMed: 7725803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Site-directed mutagenesis of the yeast mitochondrial ADP/ATP translocator. Six arginines and one lysine are essential." Nelson D.R., Lawson J.E., Klingenberg M., Douglas M.G. J. Mol. Biol. 230:1159-1170(1993) [PubMed: 8487299] [Abstract] Cited for: MUTAGENESIS.
[7]	"Function-based mapping of the yeast mitochondrial ADP/ATP translocator by selection for second site revertants." Nelson D.R., Douglas M.G. J. Mol. Biol. 230:1171-1182(1993) [PubMed: 7683726] [Abstract] Cited for: MUTAGENESIS, TOPOLOGY.
[8]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-39 AND SER-42, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77291 Genomic DNA. Translation: CAA54501.1. J04021 Genomic DNA. Translation: AAA34381.1. M34075 Genomic DNA. Translation: AAA97484.1. X74427 Genomic DNA. Translation: CAA52446.1. Z35791 Genomic DNA. Translation: CAA84850.1.
PIR	A31978.
RefSeq	NP_009523.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2917N.
IntAct	P18239. 58 interactions.
STRING	P18239.
Protein family/group databases
TCDB	2.A.29.1.7. mitochondrial carrier (MC) family.
Proteomic databases
PeptideAtlas	P18239.
PRIDE	P18239.
Genome annotation databases
Ensembl	YBL030C; YBL030C; YBL030C; Saccharomyces cerevisiae. [Genome view]
GeneID	852250.
KEGG	sce:YBL030C.
NMPDR	fig|4932.3.peg.215.
Organism-specific databases
CYGD	YBL030c.
SGD	S000000126. PET9.
Phylogenomic databases
HOGENOM	HBG610399.
OMA	ISPEAQY.
OrthoDB	EOG9WWT2K.
Gene expression databases
ArrayExpress	P18239.
Genevestigator	P18239.
GermOnline	YBL030C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002113. Aden_trnslctor. IPR002067. Mit_carrier. IPR001993. Mitochondria_substrate_carrier. IPR018108. Mitochondrial_sb/sol_carrier. [Graphical view]
PANTHER	PTHR11896. Mitoch_carrier. 1 hit.
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00927. ADPTRNSLCASE. PR00926. MITOCARRIER.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970820.

Entry information

Entry name

ADT2_YEAST

Accession

Primary (citable) accession number: P18239

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	October 1, 1994
Last modified:	December 15, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)