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P18239 (ADT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ADP,ATP carrier protein 2
Alternative name(s):
ADP/ATP translocase 2
Adenine nucleotide translocator 2
Short name=ANT 2
Petite colonies protein 9
Gene names
Name:PET9
Synonyms:AAC2
Ordered Locus Names:YBL030C
ORF Names:YBL0421
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein.

Miscellaneous

The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. At least 2 of the odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue By similarity.

Sequence similarities

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]

Contains 3 Solcar repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318ADP,ATP carrier protein 2
PRO_0000090594

Regions

Transmembrane20 – 5435Helical; Name=1; By similarity
Transmembrane91 – 11525Helical; Name=2; By similarity
Transmembrane123 – 15735Helical; Name=3; By similarity
Transmembrane192 – 21928Helical; Name=4; By similarity
Transmembrane224 – 25835Helical; Name=5; By similarity
Transmembrane287 – 31226Helical; Name=6; By similarity
Repeat21 – 11494Solcar 1
Repeat125 – 21793Solcar 2
Repeat225 – 31187Solcar 3
Motif252 – 2576Substrate recognition By similarity

Sites

Binding site961Nucleotide By similarity

Experimental info

Sequence conflict581L → I in AAA97484. Ref.2
Sequence conflict651R → K in AAA97484. Ref.2
Sequence conflict681A → S in AAA97484. Ref.2
Sequence conflict711L → V in AAA97484. Ref.2
Sequence conflict791T → K in AAA97484. Ref.2
Sequence conflict831V → L in AAA97484. Ref.2
Sequence conflict1131A → L in AAA97484. Ref.2
Sequence conflict1241A → G in AAA97484. Ref.2

Secondary structure

.................................... 318
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18239 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: A9805DC33D9E24AC

FASTA31834,426
        10         20         30         40         50         60 
MSSNAQVKTP LPPAPAPKKE SNFLIDFLMG GVSAAVAKTA ASPIERVKLL IQNQDEMLKQ 

        70         80         90        100        110        120 
GTLDRKYAGI LDCFKRTATQ EGVISFWRGN TANVIRYFPT QALNFAFKDK IKAMFGFKKE 

       130        140        150        160        170        180 
EGYAKWFAGN LASGGAAGAL SLLFVYSLDY ARTRLAADSK SSKKGGARQF NGLIDVYKKT 

       190        200        210        220        230        240 
LKSDGVAGLY RGFLPSVVGI VVYRGLYFGM YDSLKPLLLT GSLEGSFLAS FLLGWVVTTG 

       250        260        270        280        290        300 
ASTCSYPLDT VRRRMMMTSG QAVKYDGAFD CLRKIVAAEG VGSLFKGCGA NILRGVAGAG 

       310 
VISMYDQLQM ILFGKKFK 

« Hide

References

« Hide 'large scale' references
[1]"Separate genes encode functionally equivalent ADP/ATP carrier proteins in Saccharomyces cerevisiae. Isolation and analysis of AAC2."
Lawson J.E., Douglas M.G.
J. Biol. Chem. 263:14812-14818(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A third ADP/ATP translocator gene in yeast."
Kolarov J., Kolarova N., Nelson N.
J. Biol. Chem. 265:12711-12716(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]Trezeguet V., le Saux A., Lauquin G.
Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen."
van Dyck L., Jonniaux J.-L., Barreiros T.D.M., Kleine K., Goffeau A.
Yeast 10:1663-1673(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Site-directed mutagenesis of the yeast mitochondrial ADP/ATP translocator. Six arginines and one lysine are essential."
Nelson D.R., Lawson J.E., Klingenberg M., Douglas M.G.
J. Mol. Biol. 230:1159-1170(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[8]"Function-based mapping of the yeast mitochondrial ADP/ATP translocator by selection for second site revertants."
Nelson D.R., Douglas M.G.
J. Mol. Biol. 230:1171-1182(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS, TOPOLOGY.
[9]"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77291 Genomic DNA. Translation: CAA54501.1.
J04021 Genomic DNA. Translation: AAA34381.1.
M34075 Genomic DNA. Translation: AAA97484.1.
X74427 Genomic DNA. Translation: CAA52446.1.
Z35791 Genomic DNA. Translation: CAA84850.1.
BK006936 Genomic DNA. Translation: DAA07089.1.
PIRA31978.
RefSeqNP_009523.1. NM_001178270.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4C9GX-ray2.49A1-318[»]
4C9HX-ray3.20A/B1-318[»]
ProteinModelPortalP18239.
SMRP18239. Positions 22-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32667. 125 interactions.
DIPDIP-2917N.
IntActP18239. 42 interactions.
MINTMINT-1360850.
STRING4932.YBL030C.

Protein family/group databases

TCDB2.A.29.1.7. the mitochondrial carrier (mc) family.

Proteomic databases

PaxDbP18239.
PeptideAtlasP18239.
PRIDEP18239.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL030C; YBL030C; YBL030C.
GeneID852250.
KEGGsce:YBL030C.

Organism-specific databases

CYGDYBL030c.
SGDS000000126. PET9.

Phylogenomic databases

eggNOGNOG238123.
GeneTreeENSGT00390000011543.
HOGENOMHOG000165727.
KOK05863.
OMAVECFKRT.
OrthoDBEOG7NCVDV.

Enzyme and pathway databases

BioCycYEAST:G3O-28933-MONOMER.

Gene expression databases

GenevestigatorP18239.

Family and domain databases

Gene3D1.50.40.10. 1 hit.
InterProIPR002113. Aden_trnslctor.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSPR00927. ADPTRNSLCASE.
PR00926. MITOCARRIER.
SUPFAMSSF103506. SSF103506. 1 hit.
PROSITEPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio970820.

Entry information

Entry nameADT2_YEAST
AccessionPrimary (citable) accession number: P18239
Secondary accession number(s): D6VPW9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references