Reviewed, UniProtKB/Swiss-Prot P18209 (PELD_ERWCH)

Names and origin

Protein names

Recommended name:
    Pectate lyase D
    EC=4.2.2.2

Gene names

Name:

pelD

Organism

Erwinia chrysanthemi

Taxonomic identifier

556 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family. PLBC subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31
	Chain	32 – 391	360	Pectate lyase D		PRO_0000024856
Sites
	Active site	266	1	Potential
	Metal binding	170	1	Calcium By similarity
	Metal binding	213	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P18209-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: C8EEF1C9493F2ABC	FASTA	391	42,066
	10 20 30 40 50 60 MNNTRVSFRS TKSLLAAIIA TSMMTWSVNR ATLQTTKATE AASTGWATQG GTTGGAKAAS 70 80 90 100 110 120 AKIYAVKNIS EFKAALNGTD TDPKIIQVTG AIDISGGKAY TSFDDQKARS QISVPSNTTI 130 140 150 160 170 180 IGIGSNGKFT NGSLVIKGVS NVILRNLYIE TPVDVAPHYE EGDGWNAEWD AAVIDNSTRV 190 200 210 220 230 240 WVDHVTISDG SFTDDKYTTK NGEKYVQHDG ALDIKKGSDY VTISSSRFEL HDKTILIGHS 250 260 270 280 290 300 DSNGSQDSGK LRVTFHNNVF DRVTERTPRV RFGSIHAYNN VYLGDVKNSV YPYLYSFGLG 310 320 330 340 350 360 TSGTILSESN SFTLSNLKSI DGKNPECSIV KQFNSKVFSD NGSLVNGSST TKLDTCAVTA 370 380 390 YKPTLPYKYS AQTMTSSLAS SINSNAGYGK L

References

[1]	"Relationship between the pel genes of the pelADE cluster in Erwinia chrysanthemi strain B374." van Gijsegem F. Mol. Microbiol. 3:1415-1424(1989) [PubMed: 2615652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B374.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17284 Genomic DNA. Translation: CAA35176.1.
PIR	S06969.
3D structure databases
SMR	P18209. Positions 31-391.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Enzyme and pathway databases
BRENDA	4.2.2.2. 1459.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PELD_ERWCH

Accession

Primary (citable) accession number: P18209

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	January 19, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)