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P18200

- PGPA_ECOLI

UniProt

P18200 - PGPA_ECOLI

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Protein

Phosphatidylglycerophosphatase A

Gene

pgpA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG).4 Publications

Catalytic activityi

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.4 Publications

Cofactori

Magnesium.1 Publication

Pathwayi

GO - Molecular functioni

  1. lipid phosphatase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. phosphatidylglycerophosphatase activity Source: UniProtKB

GO - Biological processi

  1. glycerophospholipid biosynthetic process Source: EcoCyc
  2. phosphatidylglycerol biosynthetic process Source: UniProtKB
  3. phospholipid catabolic process Source: UniProtKB-KW
  4. phospholipid dephosphorylation Source: UniProtKB
  5. response to magnesium ion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:PGPPHOSPHAA-MONOMER.
ECOL316407:JW0408-MONOMER.
MetaCyc:PGPPHOSPHAA-MONOMER.
UniPathwayiUPA00084; UER00504.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylglycerophosphatase A (EC:3.1.3.27)
Alternative name(s):
Phosphatidylglycerolphosphate phosphatase A
Short name:
PGP phosphatase A
Gene namesi
Name:pgpA
Synonyms:yajN
Ordered Locus Names:b0418, JW0408
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10704. pgpA.

Subcellular locationi

Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of plasma membrane Source: EcoCyc
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Displays 7-10 fold higher levels of phosphatidylglycerophosphate (PGP) than wild-type. Simultaneous deletion of pgpA and pgpB leads to 40 times higher PGP levels compared to wild-type, while simultaneous deletion of pgpA and pgpC leads to almost 100 times higher PGP levels. Lethal when combined with the deletion of both pgpB and pgpC.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172Phosphatidylglycerophosphatase APRO_0000058359Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP18200.

Interactioni

Protein-protein interaction databases

STRINGi511145.b0418.

Structurei

3D structure databases

ProteinModelPortaliP18200.
SMRiP18200. Positions 84-163.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini53 – 531PeriplasmicSequence Analysis
Topological domaini75 – 14167CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini163 – 17210PeriplasmicSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei32 – 5221HelicalSequence AnalysisAdd
BLAST
Transmembranei54 – 7421HelicalSequence AnalysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1267.
HOGENOMiHOG000256112.
InParanoidiP18200.
KOiK01095.
OMAiYICDKAA.
OrthoDBiEOG6P5ZM2.
PhylomeDBiP18200.

Family and domain databases

InterProiIPR026037. PgpA.
IPR007686. YutG/PgpA.
[Graphical view]
PfamiPF04608. PgpA. 1 hit.
[Graphical view]
PIRSFiPIRSF006162. PgpA. 1 hit.
SUPFAMiSSF101307. SSF101307. 1 hit.

Sequencei

Sequence statusi: Complete.

P18200-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTILPRHKDV AKSRLKMSNP WHLLAVGFGS GLSPIVPGTM GSLAAIPFWY
60 70 80 90 100
LMTFLPWQLY SLVVMLGICI GVYLCHQTAK DMGVHDHGSI VWDEFIGMWI
110 120 130 140 150
TLMALPTNDW QWVAAGFVIF RILDMWKPWP IRWFDRNVHG GMGIMIDDIV
160 170
AGVISAGILY FIGHHWPLGI LS
Length:172
Mass (Da):19,418
Last modified:November 1, 1997 - v2
Checksum:i9DA1C817CA36C8B9
GO

Sequence cautioni

The sequence AAA24325.1 differs from that shown. Reason: Frameshift at position 42. In addition, due to translation of the incorrect DNA strand, an unrelated ORF was predicted.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23546 Genomic DNA. Translation: AAA24325.1. Sequence problems.
D17333 Genomic DNA. Translation: BAA21779.1.
U82664 Genomic DNA. Translation: AAB40174.1.
U00096 Genomic DNA. Translation: AAC73521.1.
AP009048 Genomic DNA. Translation: BAE76198.1.
PIRiA30192. PAECGA.
B64771.
RefSeqiNP_414952.1. NC_000913.3.
YP_488710.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73521; AAC73521; b0418.
BAE76198; BAE76198; BAE76198.
GeneIDi12930347.
947542.
KEGGiecj:Y75_p0406.
eco:b0418.
PATRICi32115985. VBIEscCol129921_0434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23546 Genomic DNA. Translation: AAA24325.1 . Sequence problems.
D17333 Genomic DNA. Translation: BAA21779.1 .
U82664 Genomic DNA. Translation: AAB40174.1 .
U00096 Genomic DNA. Translation: AAC73521.1 .
AP009048 Genomic DNA. Translation: BAE76198.1 .
PIRi A30192. PAECGA.
B64771.
RefSeqi NP_414952.1. NC_000913.3.
YP_488710.1. NC_007779.1.

3D structure databases

ProteinModelPortali P18200.
SMRi P18200. Positions 84-163.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b0418.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73521 ; AAC73521 ; b0418 .
BAE76198 ; BAE76198 ; BAE76198 .
GeneIDi 12930347.
947542.
KEGGi ecj:Y75_p0406.
eco:b0418.
PATRICi 32115985. VBIEscCol129921_0434.

Organism-specific databases

EchoBASEi EB0698.
EcoGenei EG10704. pgpA.

Phylogenomic databases

eggNOGi COG1267.
HOGENOMi HOG000256112.
InParanoidi P18200.
KOi K01095.
OMAi YICDKAA.
OrthoDBi EOG6P5ZM2.
PhylomeDBi P18200.

Enzyme and pathway databases

UniPathwayi UPA00084 ; UER00504 .
BioCyci EcoCyc:PGPPHOSPHAA-MONOMER.
ECOL316407:JW0408-MONOMER.
MetaCyc:PGPPHOSPHAA-MONOMER.

Miscellaneous databases

PROi P18200.

Gene expression databases

Genevestigatori P18200.

Family and domain databases

InterProi IPR026037. PgpA.
IPR007686. YutG/PgpA.
[Graphical view ]
Pfami PF04608. PgpA. 1 hit.
[Graphical view ]
PIRSFi PIRSF006162. PgpA. 1 hit.
SUPFAMi SSF101307. SSF101307. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Membrane-bound phosphatases in Escherichia coli: sequence of the pgpA gene."
    Icho T.
    J. Bacteriol. 170:5110-5116(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    Strain: K12.
  2. Iida A., Hayashi M., Fujio T., Teshiba S.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Multiple genes for membrane-bound phosphatases in Escherichia coli and their action on phospholipid precursors."
    Icho T., Raetz C.R.
    J. Bacteriol. 153:722-730(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    Strain: K12.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  8. "A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4."
    Osman C., Haag M., Wieland F.T., Brugger B., Langer T.
    EMBO J. 29:1976-1987(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  9. "Three phosphatidylglycerol-phosphate phosphatases in the inner membrane of Escherichia coli."
    Lu Y.H., Guan Z., Zhao J., Raetz C.R.
    J. Biol. Chem. 286:5506-5518(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPGPA_ECOLI
AccessioniPrimary (citable) accession number: P18200
Secondary accession number(s): P77321, Q2MC08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3