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Protein

Phosphatidylglycerophosphatase A

Gene

pgpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG).4 Publications

Catalytic activityi

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.4 Publications

Cofactori

Mg2+1 Publication

Pathwayi: phosphatidylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA)
  2. Phosphatidylglycerophosphatase C (pgpC), Phosphatidylglycerophosphatase A (pgpA), Phosphatidylglycerophosphatase B (pgpB)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • lipid phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phosphatidylglycerophosphatase activity Source: UniProtKB

GO - Biological processi

  • glycerophospholipid biosynthetic process Source: EcoCyc
  • phosphatidylglycerol biosynthetic process Source: UniProtKB
  • phospholipid catabolic process Source: UniProtKB-KW
  • phospholipid dephosphorylation Source: UniProtKB
  • response to magnesium ion Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:PGPPHOSPHAA-MONOMER
MetaCyc:PGPPHOSPHAA-MONOMER
UniPathwayiUPA00084; UER00504

Chemistry databases

SwissLipidsiSLP:000000222

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylglycerophosphatase A (EC:3.1.3.27)
Alternative name(s):
Phosphatidylglycerolphosphate phosphatase A
Short name:
PGP phosphatase A
Gene namesi
Name:pgpA
Synonyms:yajN
Ordered Locus Names:b0418, JW0408
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10704 pgpA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31CytoplasmicSequence analysisAdd BLAST31
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Topological domaini53PeriplasmicSequence analysis1
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 141CytoplasmicSequence analysisAdd BLAST67
Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
Topological domaini163 – 172PeriplasmicSequence analysis10

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: UniProtKB

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Displays 7-10 fold higher levels of phosphatidylglycerophosphate (PGP) than wild-type. Simultaneous deletion of pgpA and pgpB leads to 40 times higher PGP levels compared to wild-type, while simultaneous deletion of pgpA and pgpC leads to almost 100 times higher PGP levels. Lethal when combined with the deletion of both pgpB and pgpC.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000583591 – 172Phosphatidylglycerophosphatase AAdd BLAST172

Proteomic databases

PaxDbiP18200
PRIDEiP18200

Interactioni

Protein-protein interaction databases

BioGridi4259341, 300 interactors
STRINGi316385.ECDH10B_0374

Structurei

3D structure databases

ProteinModelPortaliP18200
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108ZAI Bacteria
COG1267 LUCA
HOGENOMiHOG000256112
InParanoidiP18200
KOiK01095
OMAiPKAPGTF
PhylomeDBiP18200

Family and domain databases

CDDicd06971 PgpA, 1 hit
InterProiView protein in InterPro
IPR026037 PgpA
IPR036681 PgpA-like_sf
IPR007686 YutG/PgpA
PANTHERiPTHR36305 PTHR36305, 1 hit
PfamiView protein in Pfam
PF04608 PgpA, 1 hit
PIRSFiPIRSF006162 PgpA, 1 hit
SUPFAMiSSF101307 SSF101307, 1 hit

Sequencei

Sequence statusi: Complete.

P18200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTILPRHKDV AKSRLKMSNP WHLLAVGFGS GLSPIVPGTM GSLAAIPFWY
60 70 80 90 100
LMTFLPWQLY SLVVMLGICI GVYLCHQTAK DMGVHDHGSI VWDEFIGMWI
110 120 130 140 150
TLMALPTNDW QWVAAGFVIF RILDMWKPWP IRWFDRNVHG GMGIMIDDIV
160 170
AGVISAGILY FIGHHWPLGI LS
Length:172
Mass (Da):19,418
Last modified:November 1, 1997 - v2
Checksum:i9DA1C817CA36C8B9
GO

Sequence cautioni

The sequence AAA24325 differs from that shown. Reason: Frameshift at position 42. In addition, due to translation of the incorrect DNA strand, an unrelated ORF was predicted.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23546 Genomic DNA Translation: AAA24325.1 Sequence problems.
D17333 Genomic DNA Translation: BAA21779.1
U82664 Genomic DNA Translation: AAB40174.1
U00096 Genomic DNA Translation: AAC73521.1
AP009048 Genomic DNA Translation: BAE76198.1
PIRiA30192 PAECGA
B64771
RefSeqiNP_414952.1, NC_000913.3
WP_000154044.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73521; AAC73521; b0418
BAE76198; BAE76198; BAE76198
GeneIDi947542
KEGGiecj:JW0408
eco:b0418
PATRICifig|1411691.4.peg.1859

Similar proteinsi

Entry informationi

Entry nameiPGPA_ECOLI
AccessioniPrimary (citable) accession number: P18200
Secondary accession number(s): P77321, Q2MC08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health