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P18200

- PGPA_ECOLI

UniProt

P18200 - PGPA_ECOLI

Protein

Phosphatidylglycerophosphatase A

Gene

pgpA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG).4 Publications

    Catalytic activityi

    Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.4 Publications

    Cofactori

    Magnesium.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. lipid phosphatase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW
    3. phosphatidylglycerophosphatase activity Source: UniProtKB

    GO - Biological processi

    1. glycerophospholipid biosynthetic process Source: EcoCyc
    2. phosphatidylglycerol biosynthetic process Source: UniProtKB
    3. phospholipid catabolic process Source: UniProtKB-KW
    4. phospholipid dephosphorylation Source: UniProtKB
    5. response to magnesium ion Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PGPPHOSPHAA-MONOMER.
    ECOL316407:JW0408-MONOMER.
    MetaCyc:PGPPHOSPHAA-MONOMER.
    UniPathwayiUPA00084; UER00504.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylglycerophosphatase A (EC:3.1.3.27)
    Alternative name(s):
    Phosphatidylglycerolphosphate phosphatase A
    Short name:
    PGP phosphatase A
    Gene namesi
    Name:pgpA
    Synonyms:yajN
    Ordered Locus Names:b0418, JW0408
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10704. pgpA.

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of plasma membrane Source: EcoCyc
    2. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Displays 7-10 fold higher levels of phosphatidylglycerophosphate (PGP) than wild-type. Simultaneous deletion of pgpA and pgpB leads to 40 times higher PGP levels compared to wild-type, while simultaneous deletion of pgpA and pgpC leads to almost 100 times higher PGP levels. Lethal when combined with the deletion of both pgpB and pgpC.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 172172Phosphatidylglycerophosphatase APRO_0000058359Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP18200.

    Interactioni

    Protein-protein interaction databases

    STRINGi511145.b0418.

    Structurei

    3D structure databases

    ProteinModelPortaliP18200.
    SMRiP18200. Positions 84-163.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3131CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini53 – 531PeriplasmicSequence Analysis
    Topological domaini75 – 14167CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini163 – 17210PeriplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei54 – 7421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei142 – 16221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1267.
    HOGENOMiHOG000256112.
    KOiK01095.
    OMAiYICDKAA.
    OrthoDBiEOG6P5ZM2.
    PhylomeDBiP18200.

    Family and domain databases

    InterProiIPR026037. PgpA.
    IPR007686. YutG/PgpA.
    [Graphical view]
    PfamiPF04608. PgpA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006162. PgpA. 1 hit.
    SUPFAMiSSF101307. SSF101307. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P18200-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTILPRHKDV AKSRLKMSNP WHLLAVGFGS GLSPIVPGTM GSLAAIPFWY    50
    LMTFLPWQLY SLVVMLGICI GVYLCHQTAK DMGVHDHGSI VWDEFIGMWI 100
    TLMALPTNDW QWVAAGFVIF RILDMWKPWP IRWFDRNVHG GMGIMIDDIV 150
    AGVISAGILY FIGHHWPLGI LS 172
    Length:172
    Mass (Da):19,418
    Last modified:November 1, 1997 - v2
    Checksum:i9DA1C817CA36C8B9
    GO

    Sequence cautioni

    The sequence AAA24325.1 differs from that shown. Reason: Frameshift at position 42. In addition, due to translation of the incorrect DNA strand, an unrelated ORF was predicted.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23546 Genomic DNA. Translation: AAA24325.1. Sequence problems.
    D17333 Genomic DNA. Translation: BAA21779.1.
    U82664 Genomic DNA. Translation: AAB40174.1.
    U00096 Genomic DNA. Translation: AAC73521.1.
    AP009048 Genomic DNA. Translation: BAE76198.1.
    PIRiA30192. PAECGA.
    B64771.
    RefSeqiNP_414952.1. NC_000913.3.
    YP_488710.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73521; AAC73521; b0418.
    BAE76198; BAE76198; BAE76198.
    GeneIDi12930347.
    947542.
    KEGGiecj:Y75_p0406.
    eco:b0418.
    PATRICi32115985. VBIEscCol129921_0434.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23546 Genomic DNA. Translation: AAA24325.1 . Sequence problems.
    D17333 Genomic DNA. Translation: BAA21779.1 .
    U82664 Genomic DNA. Translation: AAB40174.1 .
    U00096 Genomic DNA. Translation: AAC73521.1 .
    AP009048 Genomic DNA. Translation: BAE76198.1 .
    PIRi A30192. PAECGA.
    B64771.
    RefSeqi NP_414952.1. NC_000913.3.
    YP_488710.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P18200.
    SMRi P18200. Positions 84-163.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b0418.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73521 ; AAC73521 ; b0418 .
    BAE76198 ; BAE76198 ; BAE76198 .
    GeneIDi 12930347.
    947542.
    KEGGi ecj:Y75_p0406.
    eco:b0418.
    PATRICi 32115985. VBIEscCol129921_0434.

    Organism-specific databases

    EchoBASEi EB0698.
    EcoGenei EG10704. pgpA.

    Phylogenomic databases

    eggNOGi COG1267.
    HOGENOMi HOG000256112.
    KOi K01095.
    OMAi YICDKAA.
    OrthoDBi EOG6P5ZM2.
    PhylomeDBi P18200.

    Enzyme and pathway databases

    UniPathwayi UPA00084 ; UER00504 .
    BioCyci EcoCyc:PGPPHOSPHAA-MONOMER.
    ECOL316407:JW0408-MONOMER.
    MetaCyc:PGPPHOSPHAA-MONOMER.

    Miscellaneous databases

    PROi P18200.

    Gene expression databases

    Genevestigatori P18200.

    Family and domain databases

    InterProi IPR026037. PgpA.
    IPR007686. YutG/PgpA.
    [Graphical view ]
    Pfami PF04608. PgpA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006162. PgpA. 1 hit.
    SUPFAMi SSF101307. SSF101307. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Membrane-bound phosphatases in Escherichia coli: sequence of the pgpA gene."
      Icho T.
      J. Bacteriol. 170:5110-5116(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
      Strain: K12.
    2. Iida A., Hayashi M., Fujio T., Teshiba S.
      Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Multiple genes for membrane-bound phosphatases in Escherichia coli and their action on phospholipid precursors."
      Icho T., Raetz C.R.
      J. Bacteriol. 153:722-730(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
      Strain: K12.
    7. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.
    8. "A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4."
      Osman C., Haag M., Wieland F.T., Brugger B., Langer T.
      EMBO J. 29:1976-1987(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    9. "Three phosphatidylglycerol-phosphate phosphatases in the inner membrane of Escherichia coli."
      Lu Y.H., Guan Z., Zhao J., Raetz C.R.
      J. Biol. Chem. 286:5506-5518(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiPGPA_ECOLI
    AccessioniPrimary (citable) accession number: P18200
    Secondary accession number(s): P77321, Q2MC08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3