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P18196 (MINC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Septum site-determining protein MinC
Gene names
Name:minC
Ordered Locus Names:b1176, JW1165
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length231 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization. Ref.7

Subunit structure

Interacts with MinD and FtsZ; homodimer.

Disruption phenotype

In a minCDE operon disruption (minC-minD-minE), cells divide not only at midpoint but also at their poles, yielding small minicells and long rods. Loss of polar localization of several polar-localized proteins including GroEL-GroES, TnaA and YqjD. Ref.7

Sequence similarities

Belongs to the MinC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 231231Septum site-determining protein MinC HAMAP-Rule MF_00267
PRO_0000189033

Natural variations

Natural variant101G → D in minC19; reduces affinity of MinC for FtsZ.

Sequences

Sequence LengthMass (Da)Tools
P18196 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: E4283A6AD850DAD0

FASTA23124,776
        10         20         30         40         50         60 
MSNTPIELKG SSFTLSVVHL HEAEPKVIHQ ALEDKIAQAP AFLKHAPVVL NVSALEDPVN 

        70         80         90        100        110        120 
WSAMHKAVSA TGLRVIGVSG CKDAQLKAEI EKMGLPILTE GKEKAPRPAP TPQAPAQNTT 

       130        140        150        160        170        180 
PVTKTRLIDT PVRSGQRIYA PQCDLIVTSH VSAGAELIAD GNIHVYGMMR GRALAGASGD 

       190        200        210        220        230 
RETQIFCTNL MAELVSIAGE YWLSDQIPAE FYGKAARLQL VENALTVQPL N 

« Hide

References

« Hide 'large scale' references
[1]"A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli."
de Boer P.A.J., Crossley R.E., Rothfield L.I.
Cell 56:641-649(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization."
Hu Z., Mukherjee A., Pichoff S., Lutkenhaus J.
Proc. Natl. Acad. Sci. U.S.A. 96:14819-14824(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Analysis of MinC reveals two independent domains involved in interaction with MinD and FtsZ."
Hu Z., Lutkenhaus J.
J. Bacteriol. 182:3965-3971(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"Isolation and identification of new inner membrane-associated proteins that localize to cell poles in Escherichia coli."
Li G., Young K.D.
Mol. Microbiol. 84:276-295(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN LOCATION AT CELL POLES, DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03153 Genomic DNA. Translation: AAB59061.1.
U00096 Genomic DNA. Translation: AAC74260.1.
AP009048 Genomic DNA. Translation: BAA36010.1.
PIRCEECIC. A31877.
RefSeqNP_415694.1. NC_000913.3.
YP_489443.1. NC_007779.1.

3D structure databases

ProteinModelPortalP18196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10214N.
IntActP18196. 32 interactions.
MINTMINT-113907.
STRING511145.b1176.

Proteomic databases

PaxDbP18196.
PRIDEP18196.

Protocols and materials databases

DNASU945744.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74260; AAC74260; b1176.
BAA36010; BAA36010; BAA36010.
GeneID12931104.
945744.
KEGGecj:Y75_p1148.
eco:b1176.
PATRIC32117596. VBIEscCol129921_1220.

Organism-specific databases

EchoBASEEB0591.
EcoGeneEG10596. minC.

Phylogenomic databases

eggNOGCOG0850.
HOGENOMHOG000062060.
KOK03610.
OMARIYAPNC.
OrthoDBEOG606588.
PhylomeDBP18196.

Enzyme and pathway databases

BioCycEcoCyc:EG10596-MONOMER.
ECOL316407:JW1165-MONOMER.

Gene expression databases

GenevestigatorP18196.

Family and domain databases

Gene3D2.160.20.70. 1 hit.
HAMAPMF_00267. MinC.
InterProIPR016098. CAP/MinC_C.
IPR013033. MinC.
IPR007874. MinC_N.
IPR005526. Septum_form_inhib_MinC_C.
[Graphical view]
PfamPF03775. MinC_C. 1 hit.
PF05209. MinC_N. 1 hit.
[Graphical view]
SUPFAMSSF63848. SSF63848. 1 hit.
TIGRFAMsTIGR01222. minC. 1 hit.
ProtoNetSearch...

Other

PROP18196.

Entry information

Entry nameMINC_ECOLI
AccessionPrimary (citable) accession number: P18196
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 11, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene