P18196 (MINC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Septum site-determining protein MinC

Gene names

Name:	minC
Ordered Locus Names:	b1176, JW1165

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization. Ref.7
Subunit structure	Interacts with MinD and FtsZ; homodimer.
Disruption phenotype	In a minCDE operon disruption (minC-minD-minE), cells divide not only at midpoint but also at their poles, yielding small minicells and long rods. Loss of polar localization of several polar-localized proteins including GroEL-GroES, TnaA and YqjD. Ref.7
Sequence similarities	Belongs to the MinC family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Septation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	barrier septum assembly Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from experiment PubMed 2137246. Source: EcoliWiki cell morphogenesis Inferred from electronic annotation. Source: InterPro regulation of cell cycle Inferred from electronic annotation. Source: HAMAP regulation of cell division Inferred from experiment PubMed 2137246. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Septum site-determining protein MinC HAMAP-Rule MF_00267		PRO_0000189033
Natural variations
	Natural variant	10	1	G → D in minC19; reduces affinity of MinC for FtsZ.

Sequences

	Sequence		Length	Mass (Da)
	P18196 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: E4283A6AD850DAD0	FASTA	231	24,776
	10 20 30 40 50 60 MSNTPIELKG SSFTLSVVHL HEAEPKVIHQ ALEDKIAQAP AFLKHAPVVL NVSALEDPVN 70 80 90 100 110 120 WSAMHKAVSA TGLRVIGVSG CKDAQLKAEI EKMGLPILTE GKEKAPRPAP TPQAPAQNTT 130 140 150 160 170 180 PVTKTRLIDT PVRSGQRIYA PQCDLIVTSH VSAGAELIAD GNIHVYGMMR GRALAGASGD 190 200 210 220 230 RETQIFCTNL MAELVSIAGE YWLSDQIPAE FYGKAARLQL VENALTVQPL N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli." de Boer P.A.J., Crossley R.E., Rothfield L.I. Cell 56:641-649(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization." Hu Z., Mukherjee A., Pichoff S., Lutkenhaus J. Proc. Natl. Acad. Sci. U.S.A. 96:14819-14824(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Analysis of MinC reveals two independent domains involved in interaction with MinD and FtsZ." Hu Z., Lutkenhaus J. J. Bacteriol. 182:3965-3971(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Isolation and identification of new inner membrane-associated proteins that localize to cell poles in Escherichia coli." Li G., Young K.D. Mol. Microbiol. 84:276-295(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN LOCATION AT CELL POLES, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03153 Genomic DNA. Translation: AAB59061.1. U00096 Genomic DNA. Translation: AAC74260.1. AP009048 Genomic DNA. Translation: BAA36010.1.
PIR	CEECIC. A31877.
RefSeq	NP_415694.1. NC_000913.2. YP_489443.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P18196.
SMR	P18196. Positions 5-104.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10214N.
IntAct	P18196. 12 interactions.
MINT	MINT-113907.
STRING	511145.b1176.
Proteomic databases
PaxDb	P18196.
PRIDE	P18196.
Protocols and materials databases
DNASU	945744.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74260; AAC74260; b1176. BAA36010; BAA36010; BAA36010.
GeneID	12931104. 945744.
KEGG	ecj:Y75_p1148. eco:b1176.
PATRIC	32117596. VBIEscCol129921_1220.
Organism-specific databases
EchoBASE	EB0591.
EcoGene	EG10596. minC.
Phylogenomic databases
eggNOG	COG0850.
HOGENOM	HOG000062060.
KO	K03610.
OMA	DWIKLQQ.
ProtClustDB	PRK03511.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10596-MONOMER. ECOL316407:JW1165-MONOMER.
Gene expression databases
Genevestigator	P18196.
Family and domain databases
Gene3D	2.160.20.70. 1 hit.
HAMAP	MF_00267. MinC.
InterPro	IPR016098. CAP/MinC_C. IPR013033. MinC. IPR007874. MinC_N. IPR005526. Septum_form_inhib_MinC_C. [Graphical view]
Pfam	PF03775. MinC_C. 1 hit. PF05209. MinC_N. 1 hit. [Graphical view]
SUPFAM	SSF63848. MinC_C. 1 hit.
TIGRFAMs	TIGR01222. minC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MINC_ECOLI

Accession

Primary (citable) accession number: P18196

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program