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Protein

Septum site-determining protein MinC

Gene

minC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization.1 Publication

GO - Biological processi

  1. barrier septum assembly Source: UniProtKB-HAMAP
  2. cell division Source: EcoliWiki
  3. cell morphogenesis Source: InterPro
  4. regulation of cell cycle Source: UniProtKB-HAMAP
  5. regulation of cell division Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Septation

Enzyme and pathway databases

BioCyciEcoCyc:EG10596-MONOMER.
ECOL316407:JW1165-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Septum site-determining protein MinC
Gene namesi
Name:minC
Ordered Locus Names:b1176, JW1165
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10596. minC.

Pathology & Biotechi

Disruption phenotypei

In a minCDE operon disruption (minC-minD-minE), cells divide not only at midpoint but also at their poles, yielding small minicells and long rods. Loss of polar localization of several polar-localized proteins including GroEL-GroES, TnaA and YqjD.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 231231Septum site-determining protein MinCPRO_0000189033Add
BLAST

Proteomic databases

PaxDbiP18196.
PRIDEiP18196.

Expressioni

Gene expression databases

GenevestigatoriP18196.

Interactioni

Subunit structurei

Interacts with MinD and FtsZ; homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
eutNP0AEJ83EBI-554060,EBI-8767793
manAP009463EBI-554060,EBI-554045
minDP0AEZ32EBI-554060,EBI-554545
yjjWP394092EBI-554060,EBI-9132384

Protein-protein interaction databases

DIPiDIP-10214N.
IntActiP18196. 32 interactions.
MINTiMINT-113907.
STRINGi511145.b1176.

Structurei

3D structure databases

ProteinModelPortaliP18196.
SMRiP18196. Positions 4-101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MinC family.Curated

Phylogenomic databases

eggNOGiCOG0850.
HOGENOMiHOG000062060.
InParanoidiP18196.
KOiK03610.
OMAiDCDLIVT.
OrthoDBiEOG606588.
PhylomeDBiP18196.

Family and domain databases

Gene3Di2.160.20.70. 1 hit.
HAMAPiMF_00267. MinC.
InterProiIPR016098. CAP/MinC_C.
IPR013033. MinC.
IPR007874. MinC_N.
IPR005526. Septum_form_inhib_MinC_C.
[Graphical view]
PfamiPF03775. MinC_C. 1 hit.
PF05209. MinC_N. 1 hit.
[Graphical view]
SUPFAMiSSF63848. SSF63848. 1 hit.
TIGRFAMsiTIGR01222. minC. 1 hit.

Sequencei

Sequence statusi: Complete.

P18196-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNTPIELKG SSFTLSVVHL HEAEPKVIHQ ALEDKIAQAP AFLKHAPVVL
60 70 80 90 100
NVSALEDPVN WSAMHKAVSA TGLRVIGVSG CKDAQLKAEI EKMGLPILTE
110 120 130 140 150
GKEKAPRPAP TPQAPAQNTT PVTKTRLIDT PVRSGQRIYA PQCDLIVTSH
160 170 180 190 200
VSAGAELIAD GNIHVYGMMR GRALAGASGD RETQIFCTNL MAELVSIAGE
210 220 230
YWLSDQIPAE FYGKAARLQL VENALTVQPL N
Length:231
Mass (Da):24,776
Last modified:November 1, 1990 - v1
Checksum:iE4283A6AD850DAD0
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti10 – 101G → D in minC19; reduces affinity of MinC for FtsZ.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03153 Genomic DNA. Translation: AAB59061.1.
U00096 Genomic DNA. Translation: AAC74260.1.
AP009048 Genomic DNA. Translation: BAA36010.1.
PIRiA31877. CEECIC.
RefSeqiNP_415694.1. NC_000913.3.
YP_489443.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74260; AAC74260; b1176.
BAA36010; BAA36010; BAA36010.
GeneIDi12931104.
945744.
KEGGiecj:Y75_p1148.
eco:b1176.
PATRICi32117596. VBIEscCol129921_1220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03153 Genomic DNA. Translation: AAB59061.1.
U00096 Genomic DNA. Translation: AAC74260.1.
AP009048 Genomic DNA. Translation: BAA36010.1.
PIRiA31877. CEECIC.
RefSeqiNP_415694.1. NC_000913.3.
YP_489443.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP18196.
SMRiP18196. Positions 4-101.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10214N.
IntActiP18196. 32 interactions.
MINTiMINT-113907.
STRINGi511145.b1176.

Proteomic databases

PaxDbiP18196.
PRIDEiP18196.

Protocols and materials databases

DNASUi945744.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74260; AAC74260; b1176.
BAA36010; BAA36010; BAA36010.
GeneIDi12931104.
945744.
KEGGiecj:Y75_p1148.
eco:b1176.
PATRICi32117596. VBIEscCol129921_1220.

Organism-specific databases

EchoBASEiEB0591.
EcoGeneiEG10596. minC.

Phylogenomic databases

eggNOGiCOG0850.
HOGENOMiHOG000062060.
InParanoidiP18196.
KOiK03610.
OMAiDCDLIVT.
OrthoDBiEOG606588.
PhylomeDBiP18196.

Enzyme and pathway databases

BioCyciEcoCyc:EG10596-MONOMER.
ECOL316407:JW1165-MONOMER.

Miscellaneous databases

PROiP18196.

Gene expression databases

GenevestigatoriP18196.

Family and domain databases

Gene3Di2.160.20.70. 1 hit.
HAMAPiMF_00267. MinC.
InterProiIPR016098. CAP/MinC_C.
IPR013033. MinC.
IPR007874. MinC_N.
IPR005526. Septum_form_inhib_MinC_C.
[Graphical view]
PfamiPF03775. MinC_C. 1 hit.
PF05209. MinC_N. 1 hit.
[Graphical view]
SUPFAMiSSF63848. SSF63848. 1 hit.
TIGRFAMsiTIGR01222. minC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli."
    de Boer P.A.J., Crossley R.E., Rothfield L.I.
    Cell 56:641-649(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization."
    Hu Z., Mukherjee A., Pichoff S., Lutkenhaus J.
    Proc. Natl. Acad. Sci. U.S.A. 96:14819-14824(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Analysis of MinC reveals two independent domains involved in interaction with MinD and FtsZ."
    Hu Z., Lutkenhaus J.
    J. Bacteriol. 182:3965-3971(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Isolation and identification of new inner membrane-associated proteins that localize to cell poles in Escherichia coli."
    Li G., Young K.D.
    Mol. Microbiol. 84:276-295(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LOCATION AT CELL POLES, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMINC_ECOLI
AccessioniPrimary (citable) accession number: P18196
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: February 4, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.