Reviewed, UniProtKB/Swiss-Prot P18192 (PGLR1_PECCC)

Names and origin

Protein names

Recommended name:
    Endo-polygalacturonase
    EC=3.2.1.15

Gene names

Name:	peh
Synonyms:	peh-1, peh1

Organism

Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)

Taxonomic identifier

555 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subunit structure	Monomer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 402	379	Endo-polygalacturonase		PRO_0000024758
Sites
	Active site	249	1	Proton donor By similarity
	Active site	277	1	By similarity
Natural variations
	Natural variant	37	1	A → T in strain: 71.
	Natural variant	297	1	I → V in strain: 71.
	Natural variant	399	1	T → N in strain: 71.
Experimental info
	Sequence conflict	396	1	K → N in AAA57139. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18192-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 2D37097DF1AE4916	FASTA	402	42,634
	10 20 30 40 50 60 MEYQSGKRVL SLSLGLIGLF SASAFASDSR TVSEPKAPSS CTVLKADSST ATSTIQKALN 70 80 90 100 110 120 NCGQGKAVKL SAGSSSVFLS GPLSLPSGVS LLIDKGVTLR AVNNAKSFEN APSSCGVVDT 130 140 150 160 170 180 NGKGCDAFIT ATSTTNSGIY GPGTIDGQGG VKLQDKKVSW WDLAADAKVK KLKQNTPRLI 190 200 210 220 230 240 QINKSKNFTL YNVSLINSPN FHVVFSDGDG FTAWKTTIKT PSTARNTDGI DPMSSKNITI 250 260 270 280 290 300 AHSNISTGDD NVAIKAYKGR SETRNISILH NEFGTGHGMS IGSETMGVYN VTVDDLIMTG 310 320 330 340 350 360 TTNGLRIKSD KSAAGVVNGV RYSNVVMKNV AKPIVIDTVY EKKEGSNVPD WSDITFKDIT 370 380 390 400 SQTKGVVVLN GENAKKPIEV TMKNVKLTSD STWQIKNVTV KK

References

[1]	"Sequence of the peh gene of Erwinia carotovora: homology between Erwinia and plant enzymes." Hinton J.C.D., Gill D.R., Lalo D., Plastow G.S., Salmond G.P.C. Mol. Microbiol. 4:1029-1036(1990) [PubMed: 2215211] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SCRI 193.
[2]	"Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71." Liu Y., Chatterjee A., Chatterjee A.K. Appl. Environ. Microbiol. 60:2545-2552(1994) [PubMed: 8074530] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 71.
[3]	"A note on the primary structure and expression of an Erwinia carotovora polygalacturonase-encoding gene (peh1) in Escherichia coli and Saccharomyces cerevisiae." Laing E., Pretorius I.S. J. Appl. Bacteriol. 75:149-158(1993) [PubMed: 8407675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 71.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52944 Genomic DNA. Translation: CAA37119.1. L32172 Genomic DNA. Translation: AAA57139.1. M83222 Unassigned RNA. Translation: AAA03624.1.
PIR	S11773.
3D structure databases
HSSP	HSSP built from PDB template 1BHE based on UniProtKB P26509.
SMR	P18192. Positions 27-402.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 290410.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR1_PECCC

Accession

Primary (citable) accession number: P18192
Secondary accession number(s): Q08933, Q47464

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)