Reviewed, UniProtKB/Swiss-Prot P18191 (MBHL_AZOCH)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase large subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase large subunit

Gene names

Name:	hupL
Synonyms:	hupB

Organism

Azotobacter chroococcum mcd 1

Taxonomic identifier

355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Uptake hydrogenase large subunit		PRO_0000199708
Sites
	Metal binding	74	1	Nickel Potential
	Metal binding	77	1	Nickel Potential
	Metal binding	580	1	Nickel Potential
	Metal binding	583	1	Nickel Potential
Experimental info
	Sequence conflict	444 – 450	7	GLKQFLP → ASSSSAL in CAA37134. Ref.1
	Sequence conflict	457	1	L → S in CAA37134. Ref.1
	Sequence conflict	495 – 498	4	DPST → GPEH in CAA37134. Ref.1
	Sequence conflict	515 – 517	3	ALG → RSA in CAA37134. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P18191-1 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 568E6387C2E342FD	FASTA	601	66,546
	10 20 30 40 50 60 MSSLPNASQL DKSGRRIVVD PVTRIEGHMR CEVNVDANNI ITNAVSTGTM WRGLEVILKG 70 80 90 100 110 120 RDPRDAWAFV ERICGVCTGT TRWTSVRAVE DALGIQIPYN AHLIRNLMDK QLQVQDHIVP 130 140 150 160 170 180 FYHLLRLDWV NPVNALKADP KATSALPAAL AAHAKSSPGY FRHVQTRLKK FVESGATACS 190 200 210 220 230 240 PNGYWDNPAY QAPARPDLMA VAHYLEALDV QKDIVEIHTI FGGKNPHPNY MVGGVACAIN 250 260 270 280 290 300 LDDVGAAGGR STCTSLNFVL ERIHEAREFT RNVYLPDVLA VAGIYKDWLY GGGLPGHNLL 310 320 330 340 350 360 SYGTFTKVPG DKSSDLLPAG AIVGGNWDEV LPVDVRVPEE IQEFVSHSWY RYADETKGLH 370 380 390 400 410 420 PWDGVTEPKF ELGPNTKGTR TNIKELDEAH KYSWIKARAW RGHAMEVGPL ARYIIAYRSG 430 440 450 460 470 480 REYVKEQVDR SLAAFNQSTG LNLGLKQFLP STLGRTLARA LECELAVDSM LDDWQALVGN 490 500 510 520 530 540 IKAGDRATAN VEKWDPSTWP KEAKGVGINE APRGALGHWI RLKDGKIENY QAIVPTTWNG 550 560 570 580 590 600 TPRDHLGNIG AYEAALLNTR MERPDEPVEI LRTLHSFDPC LACSTHVMSP DGQELTRVKV R

References

[1]	"The identification, characterization, sequencing and mutagenesis of the genes (hupSL) encoding the small and large subunits of the H2-uptake hydrogenase of Azotobacter chroococcum." Ford C.M., Garg N., Garg R.P., Tibelius K.H., Yates M.G., Arp D.J., Seefeldt L.C. Mol. Microbiol. 4:999-1008(1990) [PubMed: 2215219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Yates M.G. Submitted (SEP-1990) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 194 AND 564.
[3]	"Sequences, organization and analysis of the hupZMNOQRTV genes from the Azotobacter chroococcum hydrogenase gene cluster." Du L., Tibelius K.H., Souza E.M., Garg R.P., Yates M.G. J. Mol. Biol. 243:549-557(1994) [PubMed: 7966281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 428-601.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52961 Genomic DNA. Translation: CAA37134.1. L25315 Genomic DNA. Translation: AAA64446.1.
PIR	S11777. S53655.
3D structure databases
HSSP	HSSP built from PDB template 2FRV based on UniProtKB P12944.
ModBase	Search...
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_AZOCH

Accession

Primary (citable) accession number: P18191
Secondary accession number(s): Q43952

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)