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Protein

Carbamoyl-phosphate synthase arginine-specific large chain

Gene

carB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 4 Mg2+ or Mn2+ ions per subunit.By similarity

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase arginine-specific small chain (carA), Carbamoyl-phosphate synthase arginine-specific large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi284Magnesium or manganese 1By similarity1
Metal bindingi298Magnesium or manganese 1By similarity1
Metal bindingi298Magnesium or manganese 2By similarity1
Metal bindingi300Magnesium or manganese 2By similarity1
Metal bindingi822Magnesium or manganese 3By similarity1
Metal bindingi834Magnesium or manganese 3By similarity1
Metal bindingi834Magnesium or manganese 4By similarity1
Metal bindingi836Magnesium or manganese 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi159 – 216ATPBy similarityAdd BLAST58
Nucleotide bindingi701 – 756ATPBy similarityAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU11240-MONOMER
SABIO-RKiP18185
UniPathwayiUPA00068; UER00171

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase arginine-specific large chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain
Gene namesi
Name:carB
Synonyms:cpaB
Ordered Locus Names:BSU11240
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001449921 – 1030Carbamoyl-phosphate synthase arginine-specific large chainAdd BLAST1030

Proteomic databases

PaxDbiP18185

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Protein-protein interaction databases

IntActiP18185, 1 interactor
MINTiP18185
STRINGi224308.Bsubs1_010100006211

Structurei

3D structure databases

ProteinModelPortaliP18185
SMRiP18185
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 327ATP-grasp 1Add BLAST195
Domaini675 – 863ATP-grasp 2Add BLAST189
Domaini925 – 1027MGS-likePROSITE-ProRule annotationAdd BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 401Carboxyphosphate synthetic domainAdd BLAST401
Regioni402 – 548Oligomerization domainAdd BLAST147
Regioni549 – 928Carbamoyl phosphate synthetic domainAdd BLAST380
Regioni929 – 1030Allosteric domainAdd BLAST102

Sequence similaritiesi

Belongs to the CarB family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0458 LUCA
HOGENOMiHOG000234582
InParanoidiP18185
KOiK01955
OMAiFADEIYF
PhylomeDBiP18185

Family and domain databases

Gene3Di1.10.1030.10, 1 hit
3.30.1490.20, 1 hit
HAMAPiMF_01210_B CPSase_L_chain_B, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR006275 CarbamoylP_synth_lsu
IPR005480 CarbamoylP_synth_lsu_oligo
IPR036897 CarbamoylP_synth_lsu_oligo_sf
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR005483 CbamoylP_synth_lsu_CPSase_dom
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF02786 CPSase_L_D2, 2 hits
PF02787 CPSase_L_D3, 1 hit
PRINTSiPR00098 CPSASE
SMARTiView protein in SMART
SM01096 CPSase_L_D3, 1 hit
SUPFAMiSSF48108 SSF48108, 1 hit
SSF52440 SSF52440, 2 hits
TIGRFAMsiTIGR01369 CPSaseII_lrg, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 2 hits
PS00866 CPSASE_1, 2 hits
PS00867 CPSASE_2, 2 hits
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

P18185-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKDTSISSI LVIGSGPIII GQAAEFDYSG TQGCIALKEE GYRVILVNSN
60 70 80 90 100
PATIMTDEAF ADEIYFEPLT AESLTAIIKK ERPDGLLANL GGQTALNLAV
110 120 130 140 150
ELEETGVLKE HGVKLLGTSV ETIQKGEDRE KFRSLMNELK QPVPESEIVD
160 170 180 190 200
NEADALHFAE SIGFPVIIRP AYTLGGKGGG IAPDKEAFTA MIKQALLASP
210 220 230 240 250
INQCLVEKSI AGFKEIEYEV MRDSNNTCIT VCNMENIDPV GVHTGDSIVV
260 270 280 290 300
APSQTLTDED YQMLRTASLT IISALDVVGG CNIQFALDPF SKQYYVIEVN
310 320 330 340 350
PRVSRSSALA SKATGYPIAK MAAKLAVGYT LDELKNPLTG STYASFEPAL
360 370 380 390 400
DYVIVKFPRW PFDKFKNADR KLGTKMKATG EVMAIERNLE AAIQKAAASL
410 420 430 440 450
ELKNIGTHLP ELSGLSIDTL WDLAITPDDR RFFVVMELLS RSVSIDDIHE
460 470 480 490 500
KTKIDPFFLH TFDNIIKLEN RLMEAGSDLS FELLKKAKEK GFSDATIASL
510 520 530 540 550
ISKTEEEVRA LRKEMGITPS FKIVDTCAAE FDAKTNYFYS TYFGETDGDI
560 570 580 590 600
SRKEKKRALI IGSGPIRIGQ GVEFDYSAVH GVLTLQELGF ETIMINNNPE
610 620 630 640 650
TVSTDYEIAD RLYFEPMTTE HIVNVAEQEN IDFAIVQFGG QTAINAAEAL
660 670 680 690 700
EKAGITLLGT SFQTLDVLED RDQFYQLLDE LGLKHAKGEI AYTKEEAASK
710 720 730 740 750
ASEIGYPVLI RPSYVIGGMG MIIVDSQAQL SQLLNDEDSM PYPILIDQYV
760 770 780 790 800
SGKEVEIDLI SDGEEVFIPT YTEHIERAGV HSGDSFAILP GPSITSGLQQ
810 820 830 840 850
GMKDAAQKIA RKLSFKGIMN IQFVIDNGNI LVLEVNPRAS RTVPVVSKVM
860 870 880 890 900
GVPMIPLATR LLAGASLKDL NPAVQNHHGV AVKFPVFSSH AIQDVDVKLG
910 920 930 940 950
PEMKSTGEGM CVAYDSNSAL KKIYTRVWSQ KGSIYLQNVP EDVKELAENA
960 970 980 990 1000
GFTIHEGTFA SWMEQEGNSL HINLSGSEEA RKERLEAMTH GIPVFTEEET
1010 1020 1030
VRAFLQSGSG HPQPVSLKDL YKKEVASCTQ
Length:1,030
Mass (Da):112,798
Last modified:July 28, 2009 - v4
Checksum:i73508761F7E25C9E
GO

Sequence cautioni

The sequence CAA37443 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti397A → G in CAA81548 (PubMed:8025667).Curated1
Sequence conflicti510A → R in CAA81548 (PubMed:8025667).Curated1
Sequence conflicti626A → R in CAA81548 (PubMed:8025667).Curated1
Sequence conflicti854 – 857MIPL → R in CAA81548 (PubMed:8025667).Curated4
Sequence conflicti879G → A in CAA37443 (PubMed:2117745).Curated1
Sequence conflicti913A → R in CAA37443 (PubMed:2117745).Curated1
Sequence conflicti1004F → S in CAA37443 (PubMed:2117745).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26919 Genomic DNA Translation: CAA81548.1
AL009126 Genomic DNA Translation: CAB12965.3
X53360 Genomic DNA Translation: CAA37443.1 Different initiation.
PIRiI40377
RefSeqiNP_389006.3, NC_000964.3
WP_003232982.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB12965; CAB12965; BSU11240
GeneIDi939353
KEGGibsu:BSU11240
PATRICifig|224308.179.peg.1209

Similar proteinsi

Entry informationi

Entry nameiCARY_BACSU
AccessioniPrimary (citable) accession number: P18185
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 28, 2009
Last modified: March 28, 2018
This is version 142 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health