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Protein

RNA polymerase principal sigma factor HrdA

Gene

hrdA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi357 – 37620H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Sigma factor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase principal sigma factor HrdA
Gene namesi
Name:hrdA
Ordered Locus Names:SCO2465
ORF Names:SC7A8.04c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396RNA polymerase principal sigma factor HrdAPRO_0000093990Add
BLAST

Interactioni

Subunit structurei

Interacts transiently with the RNA polymerase catalytic core (By similarity). Interacts with RNA polymerase-binding protein RbpA.By similarity1 Publication

Protein-protein interaction databases

STRINGi100226.SCO2465.

Structurei

3D structure databases

ProteinModelPortaliP18182.
SMRiP18182. Positions 157-230, 326-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 20014Polymerase core bindingAdd
BLAST

Sequence similaritiesi

Belongs to the sigma-70 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270273.
InParanoidiP18182.
KOiK03086.
OMAiTKGHKFS.
OrthoDBiEOG6XHC70.
PhylomeDBiP18182.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGGQRRASR LRPPTYRRRP PPAASILEVA PVQTQTLTQT DTAAGGAEPD
60 70 80 90 100
AERGVLLAMP AQPGAGAALP HPGAPVDVPE HPEPPPPTRT ESGGPSSDLF
110 120 130 140 150
RQYLREIGRI PLLSAAEEVD LARRVEAGLF AEEKLRCSPG LDDRLALDLD
160 170 180 190 200
RLVVLGRLAK RRLIEANLRL VVSVAKRYVG RGLTMLDLVQ EGNLGLIRAV
210 220 230 240 250
EKFDYARGYK FSTYATWWIR QAMSRALADQ ARTIRVPVHV VELINRVVRV
260 270 280 290 300
QRRMLQERGC EPTPQEVAAH LDLAPERVGE VLRLAQEPVS LHAPVGEEDD
310 320 330 340 350
VALGDLIEDG DAASPVESAA FLLLRQHLEA VLSTLGERER KVVQLRYGLA
360 370 380 390
DGRPRTLEEI GRLFGVTRER IRQIESKTLS KLRDHAYADQ LRGYLD
Length:396
Mass (Da):43,837
Last modified:October 18, 2001 - v2
Checksum:iEB711BA589E58FCA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti211 – 2111F → I no nucleotide entry (PubMed:3194753).Curated
Sequence conflicti364 – 3641F → G in CAA37172 (PubMed:1944221).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52980 Genomic DNA. Translation: CAA37172.1.
AL939112 Genomic DNA. Translation: CAB69753.1.
PIRiS17929.
RefSeqiNP_626707.1. NC_003888.3.
WP_003976339.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB69753; CAB69753; CAB69753.
GeneIDi1097899.
KEGGisco:SCO2465.
PATRICi23734602. VBIStrCoe124346_2506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52980 Genomic DNA. Translation: CAA37172.1.
AL939112 Genomic DNA. Translation: CAB69753.1.
PIRiS17929.
RefSeqiNP_626707.1. NC_003888.3.
WP_003976339.1. NC_003888.3.

3D structure databases

ProteinModelPortaliP18182.
SMRiP18182. Positions 157-230, 326-385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO2465.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB69753; CAB69753; CAB69753.
GeneIDi1097899.
KEGGisco:SCO2465.
PATRICi23734602. VBIStrCoe124346_2506.

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270273.
InParanoidiP18182.
KOiK03086.
OMAiTKGHKFS.
OrthoDBiEOG6XHC70.
PhylomeDBiP18182.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of genes hrdA, hrdC, and hrdD from Streptomyces coelicolor A3(2) having similarity to rpoD genes."
    Tanaka K., Shiina T., Takahashi H.
    Mol. Gen. Genet. 229:334-340(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A3(2) / NRRL B-16638.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "Multiple principal sigma factor homologs in eubacteria: identification of the 'rpoD box'."
    Tanaka K., Shiina T., Takahashi H.
    Science 242:1040-1042(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 191-242.
    Strain: A3(2) / NRRL B-16638.
  4. "The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase."
    Tabib-Salazar A., Liu B., Doughty P., Lewis R.A., Ghosh S., Parsy M.L., Simpson P.J., O'Dwyer K., Matthews S.J., Paget M.S.
    Nucleic Acids Res. 41:5679-5691(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiHRDA_STRCO
AccessioniPrimary (citable) accession number: P18182
Secondary accession number(s): Q9L2F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 18, 2001
Last modified: November 11, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.