Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P18177 (TOXB_CLODI)

Last modified May 26, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Toxin B
Gene names
Name: toxB
Synonyms: tcdB
OrganismClostridium difficile
Taxonomic identifier1496 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Hide | Top

Protein attributes

Sequence length2366 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Cytotoxin.

Sequence similarities

Belongs to the peptidase C80 family.

Contains 18 cell wall-binding repeats.

Hide | Top

Ontologies

Keywords
   DomainRepeat
   Molecular functionToxin
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 23662365Toxin B
PRO_0000072636

Regions

Domain581 – 766186Peptidase C80
Repeat1832 – 185120Cell wall-binding 1
Repeat1853 – 187220Cell wall-binding 2
Repeat1875 – 189420Cell wall-binding 3
Repeat1925 – 194420Cell wall-binding 4
Repeat1966 – 198520Cell wall-binding 5
Repeat1986 – 200520Cell wall-binding 6
Repeat2006 – 202520Cell wall-binding 7
Repeat2056 – 207520Cell wall-binding 8
Repeat2076 – 209621Cell wall-binding 9
Repeat2098 – 211720Cell wall-binding 10
Repeat2118 – 213720Cell wall-binding 11
Repeat2138 – 215720Cell wall-binding 12
Repeat2208 – 223023Cell wall-binding 13
Repeat2232 – 225120Cell wall-binding 14
Repeat2252 – 227120Cell wall-binding 15
Repeat2272 – 229120Cell wall-binding 16
Repeat2322 – 234120Cell wall-binding 17
Repeat2342 – 236120Cell wall-binding 18

Secondary structure

1 ......................................................................... 2366
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P18177-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: E1024BD8B8A56ADF

FASTA2,366269,712
        10         20         30         40         50         60 
MSLVNRKQLE KMANVRFRTQ EDEYVAILDA LEEYHNMSEN TVVEKYLKLK DINSLTDIYI 

        70         80         90        100        110        120 
DTYKKSGRNK ALKKFKEYLV TEVLELKNNN LTPVEKNLHF VWIGGQINDT AINYINQWKD 

       130        140        150        160        170        180 
VNSDYNVNVF YDSNAFLINT LKKTVVESAI NDTLESFREN LNDPRFDYNK FFRKRMEIIY 

       190        200        210        220        230        240 
DKQKNFINYY KAQREENPEL IIDDIVKTYL SNEYSKEIDE LNTYIEESLN KITQNSGNDV 

       250        260        270        280        290        300 
RNFEEFKNGE SFNLYEQELV ERWNLAAASD ILRISALKEI GGMYLDVDML PGIQPDLFES 

       310        320        330        340        350        360 
IEKPSSVTVD FWEMTKLEAI MKYKEYIPEY TSEHFDMLDE EVQSSFESVL ASKSDKSEIF 

       370        380        390        400        410        420 
SSLGDMEASP LEVKIAFNSK GIINQGLISV KDSYCSNLIV KQIENRYKIL NNSLNPAISE 

       430        440        450        460        470        480 
DNDFNTTTNT FIDSIMAEAN ADNGRFMMEL GKYLRVGFFP DVKTTINLSG PEAYAAAYQD 

       490        500        510        520        530        540 
LLMFKEGSMN IHLIEADLRN FEISKTNISQ STEQEMASLW SFDDARAKAQ FEEYKRNYFE 

       550        560        570        580        590        600 
GSLGEDDNLD FSQNIVVDKE YLLEKISSLA RSSERGYIHY IVQLQGDKIS YEAACNLFAK 

       610        620        630        640        650        660 
TPYDSVLFQK NIEDSEIAYY YNPGDGEIQE IDKYKIPSII SDRPKIKLTF IGHGKDEFNT 

       670        680        690        700        710        720 
DIFAGFDVDS LSTEIEAAID LAKEDISPKS IEINLLGCNM FSYSINVEET YPGKLLLKVK 

       730        740        750        760        770        780 
DKISELMPSI SQDSIIVSAN QYEVRINSEG RRELLDHSGE WINKEESIIK DISSKEYISF 

       790        800        810        820        830        840 
NPKENKITVK SKNLPELSTL LQEIRNNSNS SDIELEEKVM LTECEINVIS NIDTQIVEER 

       850        860        870        880        890        900 
IEEAKNLTSD SINYIKDEFK LIESISDALC DLKQQNELED SHFISFEDIS ETDEGFSIRF 

       910        920        930        940        950        960 
INKETGESIF VETEKTIFSE YANHITEEIS KIKGTIFDTV NGKLVKKVNL DTTHEVNTLN 

       970        980        990       1000       1010       1020 
AAFFIQSLIE YNSSKESLSN LSVAMKVQVY AQLFSTGLNT ITDAAKVVEL VSTALDETID 

      1030       1040       1050       1060       1070       1080 
LLPTLSEGLP IIATIIDGVS LGAAIKELSE TSDPLLRQEI EAKIGIMAVN LTTATTAIIT 

      1090       1100       1110       1120       1130       1140 
SSLGIASGFS ILLVPLAGIS AGIPSLVNNE LVLRDKATKV VDYFKHVSLV ETEGVFTLLD 

      1150       1160       1170       1180       1190       1200 
DKIMMPQDDL VISEIDFNNN SIVLGKCEIW RMEGGSGHTV TDDIDHFFSA PSITYREPHL 

      1210       1220       1230       1240       1250       1260 
SIYDVLEVQK EELDLSKDLM VLPNAPNRVF AWETGWTPGL RSLENDGTKL LDRIRDNYEG 

      1270       1280       1290       1300       1310       1320 
EFYWRYFAFI ADALITTLKP RYEDTNIRIN LDSNTRSFIV PIITTEYIRE KLSYSFYGSG 

      1330       1340       1350       1360       1370       1380 
GTYALSLSQY NMGINIELSE SDVWIIDVDN VVRDVTIESD KIKKGDLIEG ILSTLSIEEN 

      1390       1400       1410       1420       1430       1440 
KIILNSHEIN FSGEVNGSNG FVSLTFSILE GINAIIEVDL LSKSYKLLIS GELKILMLNS 

      1450       1460       1470       1480       1490       1500 
NHIQQKIDYI GFNSELQKNI PYSFVDSEGK ENGFINGSTK EGLFVSELPD VVLISKVYMD 

      1510       1520       1530       1540       1550       1560 
DSKPSFGYYS NNLKDVKVIT KDNVNILTGY YLKDDIKISL SLTLQDEKTI KLNSVHLDES 

      1570       1580       1590       1600       1610       1620 
GVAEILKFMN RKGNTNTSDS LMSFLESMNI KSIFVNFLQS NIKFILDANF IISGTTSIGQ 

      1630       1640       1650       1660       1670       1680 
FEFICDENDN IQPYFIKFNT LETNYTLYVG NRQNMIVEPN YDLDDSGDIS STVINFSQKY 

      1690       1700       1710       1720       1730       1740 
LYGIDSCVNK VVISPNIYTD EINITPVYET NNTYPEVIVL DANYINEKIN VNINDLSIRY 

      1750       1760       1770       1780       1790       1800 
VWSNDGNDFI LMSTSEENKV SQVKIRFVNV FKDKTLANKL SFNFSDKQDV PVSEIILSFT 

      1810       1820       1830       1840       1850       1860 
PSYYEDGLIG YDLGLVSLYN EKFYINNFGM MVSGLIYIND SLYYFKPPVN NLITGFVTVG 

      1870       1880       1890       1900       1910       1920 
DDKYYFNPIN GGAASIGETI IDDKNYYFNQ SGVLQTGVFS TEDGFKYFAP ANTLDENLEG 

      1930       1940       1950       1960       1970       1980 
EAIDFTGKLI IDENIYYFDD NYRGAVEWKE LDGEMHYFSP ETGKAFKGLN QIGDYKYYFN 

      1990       2000       2010       2020       2030       2040 
SDGVMQKGFV SINDNKHYFD DSGVMKVGYT EIDGKHFYFA ENGEMQIGVF NTEDGFKYFA 

      2050       2060       2070       2080       2090       2100 
HHNEDLGNEE GEEISYSGIL NFNNKIYYFD DSFTAVVGWK DLEDGSKYYF DEDTAEAYIG 

      2110       2120       2130       2140       2150       2160 
LSLINDGQYY FNDDGIMQVG FVTINDKVFY FSDSGIIESG VQNIDDNYFY IDDNGIVQIG 

      2170       2180       2190       2200       2210       2220 
VFDTSDGYKY FAPANTVNDN IYGQAVEYSG LVRVGEDVYY FGETYTIETG WIYDMENESD 

      2230       2240       2250       2260       2270       2280 
KYYFNPETKK ACKGINLIDD IKYYFDEKGI MRTGLISFEN NNYYFNENGE MQFGYINIED 

      2290       2300       2310       2320       2330       2340 
KMFYFGEDGV MQIGVFNTPD GFKYFAHQNT LDENFEGESI NYTGWLDLDE KRYYFTDEYI 

      2350       2360 
AATGSVIIDG EEYYFDPDTA QLVISE

« Hide

Hide | Top

References

[1]"Nucleotide sequence of Clostridium difficile toxin B gene."
Barroso L.A., Wang S.Z., Phelps C.J., Johnson J.L., Wilkins T.D.
Nucleic Acids Res. 18:4004-4004(1990) [PubMed: 2374729] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 4325 / VPI 10463.
[2]von Eichel-Streiber C.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 4325 / VPI 10463.
[3]"Purification and characterization of toxin B from Clostridium difficile."
Meador J. III, Tweten R.K.
Infect. Immun. 56:1708-1714(1988) [PubMed: 3384474] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-18.
[4]"Comparative sequence analysis of the Clostridium difficile toxins A and B."
von Eichel-Streiber C., Laufenberg-Feldmann R., Sartingen S., Schulze J., Sauerborn M.
Mol. Gen. Genet. 233:260-268(1992) [PubMed: 1603068] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1271-2366.
Strain: ATCC 4325 / VPI 10463.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

X53138 Genomic DNA. Translation: CAA37298.1.
X92982 Genomic DNA. Translation: CAA63562.1.
X60984 Genomic DNA. Translation: CAA43299.1.
PIRA27636.
S10317.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2BVLX-ray2.20A2-541[»]
2BVMX-ray2.55A2-541[»]
ModBaseSearch...

Protein family/group databases

CAZyGT44. Glycosyltransferase Family 44.
MEROPSC80.003.
TCDB1.C.57.1.1. clostridial cytotoxin (CCT) family.

Family and domain databases

InterProIPR018337. Cell_wall/Cho-bd_repeat.
IPR002479. Cell_wall_bd_put.
IPR007577. Glyco_trans_s_bd.
[Graphical view]
PfamPF01473. CW_binding_1. 2 hits.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
PROSITEPS51170. CW. 18 hits.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameTOXB_CLODI
AccessionPrimary (citable) accession number: P18177
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: May 26, 2009
This is version 63 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents