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Protein

Toxin B

Gene

toxB

Organism
Peptoclostridium difficile (Clostridium difficile)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cytotoxin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei653 – 6531PROSITE-ProRule annotation
Active sitei698 – 6981NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  • cysteine-type peptidase activity Source: UniProtKB-KW
  • glucosyltransferase activity Source: UniProtKB

GO - Biological processi

  • pathogenesis Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease, Toxin

Enzyme and pathway databases

BRENDAi2.4.1.B62. 13625.

Protein family/group databases

CAZyiGT44. Glycosyltransferase Family 44.
MEROPSiC80.003.
TCDBi1.C.57.1.1. the clostridial cytotoxin (cct) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin B (EC:3.4.22.-)
Gene namesi
Name:toxB
Synonyms:tcdB
OrganismiPeptoclostridium difficile (Clostridium difficile)
Taxonomic identifieri1496 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaePeptoclostridium

Subcellular locationi

GO - Cellular componenti

  • host cell plasma membrane Source: AgBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 23662365Toxin BPRO_0000072636Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP18177. 3 interactions.
MINTiMINT-2634250.
STRINGi272563.CD0660.

Structurei

Secondary structure

1
2366
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 127Combined sources
Helixi22 – 3413Combined sources
Helixi42 – 6221Combined sources
Turni63 – 653Combined sources
Helixi69 – 8921Combined sources
Beta strandi96 – 1016Combined sources
Helixi109 – 12113Combined sources
Beta strandi125 – 1317Combined sources
Helixi138 – 15720Combined sources
Helixi158 – 1614Combined sources
Beta strandi163 – 1653Combined sources
Helixi168 – 19629Combined sources
Helixi202 – 21413Combined sources
Helixi218 – 23316Combined sources
Turni234 – 2363Combined sources
Beta strandi237 – 2393Combined sources
Helixi240 – 2423Combined sources
Helixi244 – 2474Combined sources
Helixi252 – 2609Combined sources
Helixi265 – 28016Combined sources
Beta strandi282 – 2854Combined sources
Turni295 – 3006Combined sources
Helixi309 – 32416Combined sources
Helixi335 – 3373Combined sources
Helixi340 – 35112Combined sources
Helixi356 – 3583Combined sources
Beta strandi374 – 3785Combined sources
Beta strandi381 – 3899Combined sources
Helixi394 – 41926Combined sources
Helixi424 – 43815Combined sources
Turni441 – 4433Combined sources
Helixi444 – 4507Combined sources
Helixi451 – 4555Combined sources
Turni456 – 4583Combined sources
Helixi465 – 4684Combined sources
Helixi471 – 48313Combined sources
Helixi495 – 4984Combined sources
Helixi499 – 5013Combined sources
Helixi505 – 5073Combined sources
Helixi514 – 5163Combined sources
Helixi524 – 54017Combined sources
Beta strandi1835 – 18384Combined sources
Beta strandi1841 – 18466Combined sources
Turni1847 – 18504Combined sources
Beta strandi1855 – 18595Combined sources
Beta strandi1862 – 18676Combined sources
Turni1868 – 18725Combined sources
Beta strandi1877 – 18815Combined sources
Beta strandi1884 – 18885Combined sources
Beta strandi1897 – 19004Combined sources
Beta strandi1902 – 19098Combined sources
Beta strandi1927 – 19315Combined sources
Beta strandi1934 – 19396Combined sources
Turni1940 – 19423Combined sources
Beta strandi1947 – 19515Combined sources
Beta strandi1954 – 19585Combined sources
Turni1960 – 19623Combined sources
Beta strandi1968 – 19725Combined sources
Beta strandi1975 – 19795Combined sources
Beta strandi1988 – 19925Combined sources
Beta strandi1995 – 19995Combined sources
Beta strandi2008 – 20125Combined sources
Beta strandi2015 – 20195Combined sources
Beta strandi2028 – 20325Combined sources
Beta strandi2035 – 20406Combined sources
Turni2044 – 20463Combined sources
Beta strandi2058 – 20625Combined sources
Beta strandi2065 – 20695Combined sources
Beta strandi2073 – 20753Combined sources
Beta strandi2078 – 20814Combined sources
Beta strandi2087 – 20904Combined sources
Turni2092 – 20943Combined sources
Beta strandi2097 – 20993Combined sources
Beta strandi2254 – 22585Combined sources
Beta strandi2261 – 22655Combined sources
Beta strandi2274 – 22785Combined sources
Beta strandi2281 – 22855Combined sources
Beta strandi2294 – 22985Combined sources
Beta strandi2301 – 23055Combined sources
Beta strandi2324 – 23285Combined sources
Beta strandi2331 – 23355Combined sources
Beta strandi2339 – 23413Combined sources
Beta strandi2344 – 23485Combined sources
Beta strandi2351 – 23555Combined sources
Turni2357 – 23593Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BVLX-ray2.20A2-541[»]
2BVMX-ray2.55A2-542[»]
4NC2X-ray2.50A2248-2366[»]
4NP4X-ray2.89A1834-2099[»]
ProteinModelPortaliP18177.
SMRiP18177. Positions 1-541.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18177.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini567 – 774208Peptidase C80PROSITE-ProRule annotationAdd
BLAST
Repeati1832 – 185120Cell wall-binding 1Add
BLAST
Repeati1853 – 187220Cell wall-binding 2Add
BLAST
Repeati1875 – 189420Cell wall-binding 3Add
BLAST
Repeati1925 – 194420Cell wall-binding 4Add
BLAST
Repeati1966 – 198520Cell wall-binding 5Add
BLAST
Repeati1986 – 200520Cell wall-binding 6Add
BLAST
Repeati2006 – 202520Cell wall-binding 7Add
BLAST
Repeati2056 – 207520Cell wall-binding 8Add
BLAST
Repeati2076 – 209621Cell wall-binding 9Add
BLAST
Repeati2098 – 211720Cell wall-binding 10Add
BLAST
Repeati2118 – 213720Cell wall-binding 11Add
BLAST
Repeati2138 – 215720Cell wall-binding 12Add
BLAST
Repeati2208 – 223023Cell wall-binding 13Add
BLAST
Repeati2232 – 225120Cell wall-binding 14Add
BLAST
Repeati2252 – 227120Cell wall-binding 15Add
BLAST
Repeati2272 – 229120Cell wall-binding 16Add
BLAST
Repeati2322 – 234120Cell wall-binding 17Add
BLAST
Repeati2342 – 236120Cell wall-binding 18Add
BLAST

Sequence similaritiesi

Contains 18 cell wall-binding repeats.PROSITE-ProRule annotation
Contains 1 peptidase C80 domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108N67. Bacteria.
COG5263. LUCA.

Family and domain databases

InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR020974. CPD_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR024770. TcdA/TcdB_cat.
IPR024772. TcdA/TcdB_N.
IPR024769. TcdA/TcdB_pore_forming.
[Graphical view]
PfamiPF01473. CW_binding_1. 4 hits.
PF11713. Peptidase_C80. 1 hit.
PF12919. TcdA_TcdB. 1 hit.
PF12920. TcdA_TcdB_pore. 1 hit.
PF12918. TcdB_N. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51771. CGT_MARTX_CPD. 1 hit.
PS51170. CW. 18 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVNRKQLE KMANVRFRTQ EDEYVAILDA LEEYHNMSEN TVVEKYLKLK
60 70 80 90 100
DINSLTDIYI DTYKKSGRNK ALKKFKEYLV TEVLELKNNN LTPVEKNLHF
110 120 130 140 150
VWIGGQINDT AINYINQWKD VNSDYNVNVF YDSNAFLINT LKKTVVESAI
160 170 180 190 200
NDTLESFREN LNDPRFDYNK FFRKRMEIIY DKQKNFINYY KAQREENPEL
210 220 230 240 250
IIDDIVKTYL SNEYSKEIDE LNTYIEESLN KITQNSGNDV RNFEEFKNGE
260 270 280 290 300
SFNLYEQELV ERWNLAAASD ILRISALKEI GGMYLDVDML PGIQPDLFES
310 320 330 340 350
IEKPSSVTVD FWEMTKLEAI MKYKEYIPEY TSEHFDMLDE EVQSSFESVL
360 370 380 390 400
ASKSDKSEIF SSLGDMEASP LEVKIAFNSK GIINQGLISV KDSYCSNLIV
410 420 430 440 450
KQIENRYKIL NNSLNPAISE DNDFNTTTNT FIDSIMAEAN ADNGRFMMEL
460 470 480 490 500
GKYLRVGFFP DVKTTINLSG PEAYAAAYQD LLMFKEGSMN IHLIEADLRN
510 520 530 540 550
FEISKTNISQ STEQEMASLW SFDDARAKAQ FEEYKRNYFE GSLGEDDNLD
560 570 580 590 600
FSQNIVVDKE YLLEKISSLA RSSERGYIHY IVQLQGDKIS YEAACNLFAK
610 620 630 640 650
TPYDSVLFQK NIEDSEIAYY YNPGDGEIQE IDKYKIPSII SDRPKIKLTF
660 670 680 690 700
IGHGKDEFNT DIFAGFDVDS LSTEIEAAID LAKEDISPKS IEINLLGCNM
710 720 730 740 750
FSYSINVEET YPGKLLLKVK DKISELMPSI SQDSIIVSAN QYEVRINSEG
760 770 780 790 800
RRELLDHSGE WINKEESIIK DISSKEYISF NPKENKITVK SKNLPELSTL
810 820 830 840 850
LQEIRNNSNS SDIELEEKVM LTECEINVIS NIDTQIVEER IEEAKNLTSD
860 870 880 890 900
SINYIKDEFK LIESISDALC DLKQQNELED SHFISFEDIS ETDEGFSIRF
910 920 930 940 950
INKETGESIF VETEKTIFSE YANHITEEIS KIKGTIFDTV NGKLVKKVNL
960 970 980 990 1000
DTTHEVNTLN AAFFIQSLIE YNSSKESLSN LSVAMKVQVY AQLFSTGLNT
1010 1020 1030 1040 1050
ITDAAKVVEL VSTALDETID LLPTLSEGLP IIATIIDGVS LGAAIKELSE
1060 1070 1080 1090 1100
TSDPLLRQEI EAKIGIMAVN LTTATTAIIT SSLGIASGFS ILLVPLAGIS
1110 1120 1130 1140 1150
AGIPSLVNNE LVLRDKATKV VDYFKHVSLV ETEGVFTLLD DKIMMPQDDL
1160 1170 1180 1190 1200
VISEIDFNNN SIVLGKCEIW RMEGGSGHTV TDDIDHFFSA PSITYREPHL
1210 1220 1230 1240 1250
SIYDVLEVQK EELDLSKDLM VLPNAPNRVF AWETGWTPGL RSLENDGTKL
1260 1270 1280 1290 1300
LDRIRDNYEG EFYWRYFAFI ADALITTLKP RYEDTNIRIN LDSNTRSFIV
1310 1320 1330 1340 1350
PIITTEYIRE KLSYSFYGSG GTYALSLSQY NMGINIELSE SDVWIIDVDN
1360 1370 1380 1390 1400
VVRDVTIESD KIKKGDLIEG ILSTLSIEEN KIILNSHEIN FSGEVNGSNG
1410 1420 1430 1440 1450
FVSLTFSILE GINAIIEVDL LSKSYKLLIS GELKILMLNS NHIQQKIDYI
1460 1470 1480 1490 1500
GFNSELQKNI PYSFVDSEGK ENGFINGSTK EGLFVSELPD VVLISKVYMD
1510 1520 1530 1540 1550
DSKPSFGYYS NNLKDVKVIT KDNVNILTGY YLKDDIKISL SLTLQDEKTI
1560 1570 1580 1590 1600
KLNSVHLDES GVAEILKFMN RKGNTNTSDS LMSFLESMNI KSIFVNFLQS
1610 1620 1630 1640 1650
NIKFILDANF IISGTTSIGQ FEFICDENDN IQPYFIKFNT LETNYTLYVG
1660 1670 1680 1690 1700
NRQNMIVEPN YDLDDSGDIS STVINFSQKY LYGIDSCVNK VVISPNIYTD
1710 1720 1730 1740 1750
EINITPVYET NNTYPEVIVL DANYINEKIN VNINDLSIRY VWSNDGNDFI
1760 1770 1780 1790 1800
LMSTSEENKV SQVKIRFVNV FKDKTLANKL SFNFSDKQDV PVSEIILSFT
1810 1820 1830 1840 1850
PSYYEDGLIG YDLGLVSLYN EKFYINNFGM MVSGLIYIND SLYYFKPPVN
1860 1870 1880 1890 1900
NLITGFVTVG DDKYYFNPIN GGAASIGETI IDDKNYYFNQ SGVLQTGVFS
1910 1920 1930 1940 1950
TEDGFKYFAP ANTLDENLEG EAIDFTGKLI IDENIYYFDD NYRGAVEWKE
1960 1970 1980 1990 2000
LDGEMHYFSP ETGKAFKGLN QIGDYKYYFN SDGVMQKGFV SINDNKHYFD
2010 2020 2030 2040 2050
DSGVMKVGYT EIDGKHFYFA ENGEMQIGVF NTEDGFKYFA HHNEDLGNEE
2060 2070 2080 2090 2100
GEEISYSGIL NFNNKIYYFD DSFTAVVGWK DLEDGSKYYF DEDTAEAYIG
2110 2120 2130 2140 2150
LSLINDGQYY FNDDGIMQVG FVTINDKVFY FSDSGIIESG VQNIDDNYFY
2160 2170 2180 2190 2200
IDDNGIVQIG VFDTSDGYKY FAPANTVNDN IYGQAVEYSG LVRVGEDVYY
2210 2220 2230 2240 2250
FGETYTIETG WIYDMENESD KYYFNPETKK ACKGINLIDD IKYYFDEKGI
2260 2270 2280 2290 2300
MRTGLISFEN NNYYFNENGE MQFGYINIED KMFYFGEDGV MQIGVFNTPD
2310 2320 2330 2340 2350
GFKYFAHQNT LDENFEGESI NYTGWLDLDE KRYYFTDEYI AATGSVIIDG
2360
EEYYFDPDTA QLVISE
Length:2,366
Mass (Da):269,712
Last modified:January 23, 2007 - v3
Checksum:iE1024BD8B8A56ADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53138 Genomic DNA. Translation: CAA37298.1.
X92982 Genomic DNA. Translation: CAA63562.1.
X60984 Genomic DNA. Translation: CAA43299.1.
PIRiA27636.
S10317.
RefSeqiWP_009902069.1. NZ_LN831031.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53138 Genomic DNA. Translation: CAA37298.1.
X92982 Genomic DNA. Translation: CAA63562.1.
X60984 Genomic DNA. Translation: CAA43299.1.
PIRiA27636.
S10317.
RefSeqiWP_009902069.1. NZ_LN831031.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BVLX-ray2.20A2-541[»]
2BVMX-ray2.55A2-542[»]
4NC2X-ray2.50A2248-2366[»]
4NP4X-ray2.89A1834-2099[»]
ProteinModelPortaliP18177.
SMRiP18177. Positions 1-541.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP18177. 3 interactions.
MINTiMINT-2634250.
STRINGi272563.CD0660.

Protein family/group databases

CAZyiGT44. Glycosyltransferase Family 44.
MEROPSiC80.003.
TCDBi1.C.57.1.1. the clostridial cytotoxin (cct) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108N67. Bacteria.
COG5263. LUCA.

Enzyme and pathway databases

BRENDAi2.4.1.B62. 13625.

Miscellaneous databases

EvolutionaryTraceiP18177.

Family and domain databases

InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR020974. CPD_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR024770. TcdA/TcdB_cat.
IPR024772. TcdA/TcdB_N.
IPR024769. TcdA/TcdB_pore_forming.
[Graphical view]
PfamiPF01473. CW_binding_1. 4 hits.
PF11713. Peptidase_C80. 1 hit.
PF12919. TcdA_TcdB. 1 hit.
PF12920. TcdA_TcdB_pore. 1 hit.
PF12918. TcdB_N. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS51771. CGT_MARTX_CPD. 1 hit.
PS51170. CW. 18 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOXB_PEPDI
AccessioniPrimary (citable) accession number: P18177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.