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P18163

- ACSL1_RAT

UniProt

P18163 - ACSL1_RAT

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Protein

Long-chain-fatty-acid--CoA ligase 1

Gene
Acsl1, Acs1, Acsl2, Facl2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses oleate, arachidonate, eicosapentaenoate and docosahexaenoate as substrates.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Magnesium.

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: RGD
  2. ATP binding Source: UniProtKB-KW
  3. long-chain fatty acid-CoA ligase activity Source: UniProtKB

GO - Biological processi

  1. adiponectin-activated signaling pathway Source: Ensembl
  2. fatty acid metabolic process Source: RGD
  3. fatty acid transport Source: RGD
  4. lipid biosynthetic process Source: Ensembl
  5. lipid metabolic process Source: RGD
  6. long-chain fatty acid import Source: Ensembl
  7. positive regulation of protein serine/threonine kinase activity Source: Ensembl
  8. response to drug Source: RGD
  9. response to nutrient Source: RGD
  10. response to oleic acid Source: RGD
  11. response to organic cyclic compound Source: RGD
  12. response to organic substance Source: RGD
  13. triglyceride metabolic process Source: RGD
  14. xenobiotic catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14442.
BRENDAi6.2.1.3. 5301.
ReactomeiREACT_196408. PPARA activates gene expression.
REACT_198818. alpha-linolenic acid (ALA) metabolism.
REACT_198820. Linoleic acid (LA) metabolism.
SABIO-RKP18163.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 1
Short name:
LACS 1
Long-chain-fatty-acid--CoA ligase, liver isozyme
Gene namesi
Name:Acsl1
Synonyms:Acs1, Acsl2, Facl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 16

Organism-specific databases

RGDi2015. Acsl1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei25 – 4521Helical; Signal-anchor for type III membrane protein; Reviewed predictionAdd
BLAST
Topological domaini46 – 699654Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: RGD
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. mitochondrial outer membrane Source: RGD
  5. peroxisomal membrane Source: HGNC
  6. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 699699Long-chain-fatty-acid--CoA ligase 1PRO_0000193106Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei9 – 91Nitrated tyrosine1 Publication
Modified residuei85 – 851Phosphotyrosine1 Publication
Modified residuei86 – 861Nitrated tyrosine1 Publication
Glycosylationi136 – 1361O-linked (GlcNAc)
Modified residuei208 – 2081N6-acetyllysine By similarity
Modified residuei357 – 3571N6-acetyllysine By similarity
Modified residuei387 – 3871N6-acetyllysine By similarity
Modified residuei621 – 6211Phosphoserine By similarity
Modified residuei633 – 6331N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP18163.
PRIDEiP18163.

PTM databases

PhosphoSiteiP18163.

Expressioni

Tissue specificityi

Liver, heart, epididymal adipose and to a lesser extent brain, small intestine and lung.

Developmental stagei

Levels remain constant during development.

Inductioni

By high fat and high carbohydrate diets.

Gene expression databases

GenevestigatoriP18163.

Interactioni

Protein-protein interaction databases

IntActiP18163. 28 interactions.
MINTiMINT-4570488.
STRINGi10116.ENSRNOP00000014235.

Structurei

3D structure databases

ProteinModelPortaliP18163.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000101725.
HOGENOMiHOG000159459.
HOVERGENiHBG050452.
InParanoidiP18163.
KOiK01897.
OMAiWDRLIFH.
OrthoDBiEOG71CFKN.
PhylomeDBiP18163.
TreeFamiTF313877.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18163-1 [UniParc]FASTAAdd to Basket

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MEVHELFRYF RMPELIDIRQ YVRTLPTNTL MGFGAFAALT TFWYATRPKA    50
LKPPCDLSMQ SVEVTGTTEG VRRSAVLEDD KLLLYYYDDV RTMYDGFQRG 100
IQVSNDGPCL GSRKPNQPYE WISYKQVAEM AECIGSALIQ KGFKPCSEQF 150
IGIFSQNRPE WVTIEQGCFT YSMVVVPLYD TLGTDAITYI VNKAELSVIF 200
ADKPEKAKLL LEGVENKLTP CLKIIVIMDS YDNDLVERGQ KCGVEIIGLK 250
ALEDLGRVNR TKPKPPEPED LAIICFTSGT TGNPKGAMVT HQNIMNDCSG 300
FIKATESAFI ASPEDVLISF LPLAHMFETV VECVMLCHGA KIGFFQGDIR 350
LLMDDLKVLQ PTIFPVVPRL LNRMFDRIFG QANTSVKRWL LDFASKRKEA 400
ELRSGIVRNN SLWDKLIFHK IQSSLGGKVR LMITGAAPVS ATVLTFLRAA 450
LGCQFYEGYG QTECTAGCCL SLPGDWTAGH VGAPMPCNYI KLVDVEDMNY 500
QAAKGEGEVC VKGANVFKGY LKDPARTAEA LDKDGWLHTG DIGKWLPNGT 550
LKIIDRKKHI FKLAQGEYIA PEKIENIYLR SEAVAQVFVH GESLQAFLIA 600
IVVPDVEILP SWAQKRGFQG SFEELCRNKD INKAILEDMV KLGKNAGLKP 650
FEQVKGIAVH PELFSIDNGL LTPTLKAKRP ELRNYFRSQI DELYSTIKI 699
Length:699
Mass (Da):78,179
Last modified:November 1, 1990 - v1
Checksum:i6E1EACE0EAE8A85C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90109 mRNA. Translation: BAA14136.1.
PIRiA36275.
RefSeqiNP_036952.1. NM_012820.1.
XP_006253184.1. XM_006253122.1.
XP_006253185.1. XM_006253123.1.
XP_006253186.1. XM_006253124.1.
XP_006253187.1. XM_006253125.1.
XP_006253188.1. XM_006253126.1.
XP_006253189.1. XM_006253127.1.
UniGeneiRn.6215.

Genome annotation databases

EnsembliENSRNOT00000014235; ENSRNOP00000014235; ENSRNOG00000010633.
GeneIDi25288.
KEGGirno:25288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90109 mRNA. Translation: BAA14136.1 .
PIRi A36275.
RefSeqi NP_036952.1. NM_012820.1.
XP_006253184.1. XM_006253122.1.
XP_006253185.1. XM_006253123.1.
XP_006253186.1. XM_006253124.1.
XP_006253187.1. XM_006253125.1.
XP_006253188.1. XM_006253126.1.
XP_006253189.1. XM_006253127.1.
UniGenei Rn.6215.

3D structure databases

ProteinModelPortali P18163.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P18163. 28 interactions.
MINTi MINT-4570488.
STRINGi 10116.ENSRNOP00000014235.

PTM databases

PhosphoSitei P18163.

Proteomic databases

PaxDbi P18163.
PRIDEi P18163.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000014235 ; ENSRNOP00000014235 ; ENSRNOG00000010633 .
GeneIDi 25288.
KEGGi rno:25288.

Organism-specific databases

CTDi 2180.
RGDi 2015. Acsl1.

Phylogenomic databases

eggNOGi COG1022.
GeneTreei ENSGT00690000101725.
HOGENOMi HOG000159459.
HOVERGENi HBG050452.
InParanoidi P18163.
KOi K01897.
OMAi WDRLIFH.
OrthoDBi EOG71CFKN.
PhylomeDBi P18163.
TreeFami TF313877.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-14442.
BRENDAi 6.2.1.3. 5301.
Reactomei REACT_196408. PPARA activates gene expression.
REACT_198818. alpha-linolenic acid (ALA) metabolism.
REACT_198820. Linoleic acid (LA) metabolism.
SABIO-RK P18163.

Miscellaneous databases

NextBioi 606025.
PROi P18163.

Gene expression databases

Genevestigatori P18163.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and regulation of rat long-chain acyl-CoA synthetase."
    Suzuki H., Kawarabayasi Y., Kondo J., Abe T., Nishikawa K., Kimura S., Hashimoto T., Yamamoto T.
    J. Biol. Chem. 265:8681-8685(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Wistar.
    Tissue: Liver.
  2. "Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry."
    Distler A.M., Kerner J., Hoppel C.L.
    Biochim. Biophys. Acta 1774:628-636(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-19; 82-91 AND 628-641, ACETYLATION AT MET-1 AND LYS-633, NITRATION AT TYR-9 AND TYR-86, PHOSPHORYLATION AT TYR-85, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Liver.
  3. "Biochemical studies of two rat acyl-CoA synthetases, ACS1 and ACS2."
    Iijima H., Fujino T., Minekura H., Suzuki H., Kang M.-J., Yamamoto T.T.
    Eur. J. Biochem. 242:186-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Discovery and confirmation of O-GlcNAcylated proteins in rat liver mitochondria by combination of mass spectrometry and immunological methods."
    Cao W., Cao J., Huang J., Yao J., Yan G., Xu H., Yang P.
    PLoS ONE 8:E76399-E76399(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-136.

Entry informationi

Entry nameiACSL1_RAT
AccessioniPrimary (citable) accession number: P18163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: September 3, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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