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P18161

- SPLB_DICDI

UniProt

P18161 - SPLB_DICDI

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Protein
Dual specificity protein kinase splB
Gene
splB, DpyK2, pyk2, pykB, DDB_G0285321
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei880 – 8801ATP By similarity
Active sitei977 – 9771Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi859 – 8679ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein binding Source: dictyBase
  4. protein tyrosine kinase activity Source: dictyBase
Complete GO annotation...

GO - Biological processi

  1. positive regulation of STAT protein import into nucleus Source: dictyBase
  2. tyrosine phosphorylation of STAT protein Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase splB (EC:2.7.10.2)
Alternative name(s):
Non-receptor tyrosine kinase spore lysis B
Tyrosine-protein kinase 2
Gene namesi
Name:splB
Synonyms:DpyK2, pyk2, pykB
ORF Names:DDB_G0285321
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285321. splB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed Reviewed prediction
Chaini2 – 11551154Dual specificity protein kinase splB
PRO_0000088122Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine Reviewed prediction

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0191483.

Structurei

3D structure databases

ProteinModelPortaliP18161.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini853 – 1126274Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi103 – 12422Gln-rich
Add
BLAST
Compositional biasi142 – 746605Asn-rich
Add
BLAST
Compositional biasi709 – 75850Thr-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
OMAiASIVCEN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18161-1 [UniParc]FASTAAdd to Basket

« Hide

MGITSSKPVS GSNTTTNSPT NQKAISEKKQ KPKRTNKRNL STDSKSSTCS     50
SPTNNYLADE LVVDLNSMEI EGADSIQYSG HSHSSSMTNS MTNSMRNSAN 100
EKQLQQKQQQ PQQQPQQQQP QPHQSHDDLN SSALDQDDGE FNNNFENESE 150
VESISSSSIN NNNTNNNSNN NNNNNNNNNN NNNNNNNIPS ISNISNNNIN 200
NNINNNNNNN SCKQKNSKNK KNKNKNNNNK NSSSSNERPY SISYDNEGST 250
TKSNSTLSLY SNISNLHSIT SHSIQDASIV CENCNHHMTK PIHLLSLSLE 300
PTSAASLDIH RNGNFSITLA SNSRSSSPAT ILQQNNKHFL INSNINIINT 350
SLNNNNKNKD EDNVNNNNGN NSNNNNNINN NNDEDNDEDN DENNNNNNNN 400
NNNNNNNNNN NNNNTSNNDI PDLSWTNYNS DSFNNNNNNN NIFNTFLSIS 450
SPISDQQNTS PQIQEIISPL PSPPTVQQSD VIQQTTTTSP SPLPSPQKQK 500
QQQQRPLQKS SPPPTTITTS TTTTTQFNNN NLASSLGSTS DLFNKSLISS 550
FLNGQGISSI NNSNPFENSY KNQINSSTNS GTNTFSNTNT NINTTNSSNT 600
NVGYDQYSFN YSNGSNSNIN NSNNGLKSFL SNSNSSFSAL KNCSYDAKDM 650
SSFTITLSNS ELNTMIAESL NRSSASNSIV KSSNTVHTTS GGNNSLCSDG 700
YNQNIISATT NTNTTTTNSS STTTTTTATT TTASTTNNNI NNNNNNSTST 750
TNSTKDITFL VCDNPDSTKE KSNVSNTSSI ISASNLNRHI TPNSHMRPRG 800
RSISESLIMS PINKESLNDI QRAIESEKIK KTKFEELKSI LGEREYIIDI 850
NDIQFIQKVG EGAFSEVWEG WWKGIHVAIK KLKIIGDEEQ FKERFIREVQ 900
NLKKGNHQNI VMFIGACYKP ACIITEYMAG GSLYNILHNP NSSTPKVKYS 950
FPLVLKMATD MALGLLHLHS ITIVHRDLTS QNILLDELGN IKISDFGLSR 1000
EKSREGSMTM TNGGICNPRW RPPELTKNLG HYSEKVDVYC FSLVVWEILT 1050
GEIPFSDLDG SQASAQVAYA GLRPPIPEYC DPELKLLLTQ CWEADPNDRP 1100
PFTYIVNKLK EISWNNPIGF VSDQFYQYSE PSTPRLALSN QSSNSSSISL 1150
SPTKL 1155
Length:1,155
Mass (Da):127,437
Last modified:December 4, 2007 - v2
Checksum:i2A00D04A39262BC2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti746 – 7494NSTS → RFYN in AAA33203. 1 Publication
Sequence conflicti1000 – 10001R → A in AAA33203. 1 Publication
Sequence conflicti1063 – 10631A → R in AAA33203. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000079 Genomic DNA. Translation: EAL64735.1.
M33784 mRNA. Translation: AAA33203.1.
PIRiB35670.
RefSeqiXP_638253.1. XM_633161.1.

Genome annotation databases

EnsemblProtistsiDDB0191483; DDB0191483; DDB_G0285321.
GeneIDi8625061.
KEGGiddi:DDB_G0285321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000079 Genomic DNA. Translation: EAL64735.1 .
M33784 mRNA. Translation: AAA33203.1 .
PIRi B35670.
RefSeqi XP_638253.1. XM_633161.1.

3D structure databases

ProteinModelPortali P18161.
ModBasei Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191483.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191483 ; DDB0191483 ; DDB_G0285321 .
GeneIDi 8625061.
KEGGi ddi:DDB_G0285321.

Organism-specific databases

dictyBasei DDB_G0285321. splB.

Phylogenomic databases

eggNOGi COG0515.
OMAi ASIVCEN.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
[Graphical view ]
PRINTSi PR00109. TYRKINASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum."
    Tan J.L., Spudich J.A.
    Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 746-1155.

Entry informationi

Entry nameiSPLB_DICDI
AccessioniPrimary (citable) accession number: P18161
Secondary accession number(s): Q54NC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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