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P18161 (SPLB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity protein kinase splB

EC=2.7.10.2
Alternative name(s):
Non-receptor tyrosine kinase spore lysis B
Tyrosine-protein kinase 2
Gene names
Name:splB
Synonyms:DpyK2, pyk2, pykB
ORF Names:DDB_G0285321
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1155 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Tyr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Potential
Chain2 – 11551154Dual specificity protein kinase splB
PRO_0000088122

Regions

Domain853 – 1126274Protein kinase
Nucleotide binding859 – 8679ATP By similarity
Compositional bias103 – 12422Gln-rich
Compositional bias142 – 746605Asn-rich
Compositional bias709 – 75850Thr-rich

Sites

Active site9771Proton acceptor By similarity
Binding site8801ATP By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine Potential

Experimental info

Sequence conflict746 – 7494NSTS → RFYN in AAA33203. Ref.2
Sequence conflict10001R → A in AAA33203. Ref.2
Sequence conflict10631A → R in AAA33203. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P18161 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 2A00D04A39262BC2

FASTA1,155127,437
        10         20         30         40         50         60 
MGITSSKPVS GSNTTTNSPT NQKAISEKKQ KPKRTNKRNL STDSKSSTCS SPTNNYLADE 

        70         80         90        100        110        120 
LVVDLNSMEI EGADSIQYSG HSHSSSMTNS MTNSMRNSAN EKQLQQKQQQ PQQQPQQQQP 

       130        140        150        160        170        180 
QPHQSHDDLN SSALDQDDGE FNNNFENESE VESISSSSIN NNNTNNNSNN NNNNNNNNNN 

       190        200        210        220        230        240 
NNNNNNNIPS ISNISNNNIN NNINNNNNNN SCKQKNSKNK KNKNKNNNNK NSSSSNERPY 

       250        260        270        280        290        300 
SISYDNEGST TKSNSTLSLY SNISNLHSIT SHSIQDASIV CENCNHHMTK PIHLLSLSLE 

       310        320        330        340        350        360 
PTSAASLDIH RNGNFSITLA SNSRSSSPAT ILQQNNKHFL INSNINIINT SLNNNNKNKD 

       370        380        390        400        410        420 
EDNVNNNNGN NSNNNNNINN NNDEDNDEDN DENNNNNNNN NNNNNNNNNN NNNNTSNNDI 

       430        440        450        460        470        480 
PDLSWTNYNS DSFNNNNNNN NIFNTFLSIS SPISDQQNTS PQIQEIISPL PSPPTVQQSD 

       490        500        510        520        530        540 
VIQQTTTTSP SPLPSPQKQK QQQQRPLQKS SPPPTTITTS TTTTTQFNNN NLASSLGSTS 

       550        560        570        580        590        600 
DLFNKSLISS FLNGQGISSI NNSNPFENSY KNQINSSTNS GTNTFSNTNT NINTTNSSNT 

       610        620        630        640        650        660 
NVGYDQYSFN YSNGSNSNIN NSNNGLKSFL SNSNSSFSAL KNCSYDAKDM SSFTITLSNS 

       670        680        690        700        710        720 
ELNTMIAESL NRSSASNSIV KSSNTVHTTS GGNNSLCSDG YNQNIISATT NTNTTTTNSS 

       730        740        750        760        770        780 
STTTTTTATT TTASTTNNNI NNNNNNSTST TNSTKDITFL VCDNPDSTKE KSNVSNTSSI 

       790        800        810        820        830        840 
ISASNLNRHI TPNSHMRPRG RSISESLIMS PINKESLNDI QRAIESEKIK KTKFEELKSI 

       850        860        870        880        890        900 
LGEREYIIDI NDIQFIQKVG EGAFSEVWEG WWKGIHVAIK KLKIIGDEEQ FKERFIREVQ 

       910        920        930        940        950        960 
NLKKGNHQNI VMFIGACYKP ACIITEYMAG GSLYNILHNP NSSTPKVKYS FPLVLKMATD 

       970        980        990       1000       1010       1020 
MALGLLHLHS ITIVHRDLTS QNILLDELGN IKISDFGLSR EKSREGSMTM TNGGICNPRW 

      1030       1040       1050       1060       1070       1080 
RPPELTKNLG HYSEKVDVYC FSLVVWEILT GEIPFSDLDG SQASAQVAYA GLRPPIPEYC 

      1090       1100       1110       1120       1130       1140 
DPELKLLLTQ CWEADPNDRP PFTYIVNKLK EISWNNPIGF VSDQFYQYSE PSTPRLALSN 

      1150 
QSSNSSSISL SPTKL 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum."
Tan J.L., Spudich J.A.
Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 746-1155.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000079 Genomic DNA. Translation: EAL64735.1.
M33784 mRNA. Translation: AAA33203.1.
PIRB35670.
RefSeqXP_638253.1. XM_633161.1.

3D structure databases

ProteinModelPortalP18161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191483.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191483; DDB0191483; DDB_G0285321.
GeneID8625061.
KEGGddi:DDB_G0285321.

Organism-specific databases

dictyBaseDDB_G0285321. splB.

Phylogenomic databases

eggNOGCOG0515.
OMAASIVCEN.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPLB_DICDI
AccessionPrimary (citable) accession number: P18161
Secondary accession number(s): Q54NC7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase