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P18161

- SPLB_DICDI

UniProt

P18161 - SPLB_DICDI

Protein

Dual specificity protein kinase splB

Gene

splB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei880 – 8801ATPPROSITE-ProRule annotation
    Active sitei977 – 9771Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi859 – 8679ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein binding Source: dictyBase
    4. protein tyrosine kinase activity Source: dictyBase

    GO - Biological processi

    1. positive regulation of STAT protein import into nucleus Source: dictyBase
    2. tyrosine phosphorylation of STAT protein Source: dictyBase

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein kinase splB (EC:2.7.10.2)
    Alternative name(s):
    Non-receptor tyrosine kinase spore lysis B
    Tyrosine-protein kinase 2
    Gene namesi
    Name:splB
    Synonyms:DpyK2, pyk2, pykB
    ORF Names:DDB_G0285321
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0285321. splB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedSequence Analysis
    Chaini2 – 11551154Dual specificity protein kinase splBPRO_0000088122Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Myristate

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0191483.

    Structurei

    3D structure databases

    ProteinModelPortaliP18161.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini853 – 1126274Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi103 – 12422Gln-richAdd
    BLAST
    Compositional biasi142 – 746605Asn-richAdd
    BLAST
    Compositional biasi709 – 75850Thr-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    OMAiASIVCEN.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18161-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGITSSKPVS GSNTTTNSPT NQKAISEKKQ KPKRTNKRNL STDSKSSTCS     50
    SPTNNYLADE LVVDLNSMEI EGADSIQYSG HSHSSSMTNS MTNSMRNSAN 100
    EKQLQQKQQQ PQQQPQQQQP QPHQSHDDLN SSALDQDDGE FNNNFENESE 150
    VESISSSSIN NNNTNNNSNN NNNNNNNNNN NNNNNNNIPS ISNISNNNIN 200
    NNINNNNNNN SCKQKNSKNK KNKNKNNNNK NSSSSNERPY SISYDNEGST 250
    TKSNSTLSLY SNISNLHSIT SHSIQDASIV CENCNHHMTK PIHLLSLSLE 300
    PTSAASLDIH RNGNFSITLA SNSRSSSPAT ILQQNNKHFL INSNINIINT 350
    SLNNNNKNKD EDNVNNNNGN NSNNNNNINN NNDEDNDEDN DENNNNNNNN 400
    NNNNNNNNNN NNNNTSNNDI PDLSWTNYNS DSFNNNNNNN NIFNTFLSIS 450
    SPISDQQNTS PQIQEIISPL PSPPTVQQSD VIQQTTTTSP SPLPSPQKQK 500
    QQQQRPLQKS SPPPTTITTS TTTTTQFNNN NLASSLGSTS DLFNKSLISS 550
    FLNGQGISSI NNSNPFENSY KNQINSSTNS GTNTFSNTNT NINTTNSSNT 600
    NVGYDQYSFN YSNGSNSNIN NSNNGLKSFL SNSNSSFSAL KNCSYDAKDM 650
    SSFTITLSNS ELNTMIAESL NRSSASNSIV KSSNTVHTTS GGNNSLCSDG 700
    YNQNIISATT NTNTTTTNSS STTTTTTATT TTASTTNNNI NNNNNNSTST 750
    TNSTKDITFL VCDNPDSTKE KSNVSNTSSI ISASNLNRHI TPNSHMRPRG 800
    RSISESLIMS PINKESLNDI QRAIESEKIK KTKFEELKSI LGEREYIIDI 850
    NDIQFIQKVG EGAFSEVWEG WWKGIHVAIK KLKIIGDEEQ FKERFIREVQ 900
    NLKKGNHQNI VMFIGACYKP ACIITEYMAG GSLYNILHNP NSSTPKVKYS 950
    FPLVLKMATD MALGLLHLHS ITIVHRDLTS QNILLDELGN IKISDFGLSR 1000
    EKSREGSMTM TNGGICNPRW RPPELTKNLG HYSEKVDVYC FSLVVWEILT 1050
    GEIPFSDLDG SQASAQVAYA GLRPPIPEYC DPELKLLLTQ CWEADPNDRP 1100
    PFTYIVNKLK EISWNNPIGF VSDQFYQYSE PSTPRLALSN QSSNSSSISL 1150
    SPTKL 1155
    Length:1,155
    Mass (Da):127,437
    Last modified:December 4, 2007 - v2
    Checksum:i2A00D04A39262BC2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti746 – 7494NSTS → RFYN in AAA33203. (PubMed:1972546)Curated
    Sequence conflicti1000 – 10001R → A in AAA33203. (PubMed:1972546)Curated
    Sequence conflicti1063 – 10631A → R in AAA33203. (PubMed:1972546)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000079 Genomic DNA. Translation: EAL64735.1.
    M33784 mRNA. Translation: AAA33203.1.
    PIRiB35670.
    RefSeqiXP_638253.1. XM_633161.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191483; DDB0191483; DDB_G0285321.
    GeneIDi8625061.
    KEGGiddi:DDB_G0285321.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000079 Genomic DNA. Translation: EAL64735.1 .
    M33784 mRNA. Translation: AAA33203.1 .
    PIRi B35670.
    RefSeqi XP_638253.1. XM_633161.1.

    3D structure databases

    ProteinModelPortali P18161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191483.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191483 ; DDB0191483 ; DDB_G0285321 .
    GeneIDi 8625061.
    KEGGi ddi:DDB_G0285321.

    Organism-specific databases

    dictyBasei DDB_G0285321. splB.

    Phylogenomic databases

    eggNOGi COG0515.
    OMAi ASIVCEN.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum."
      Tan J.L., Spudich J.A.
      Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 746-1155.

    Entry informationi

    Entry nameiSPLB_DICDI
    AccessioniPrimary (citable) accession number: P18161
    Secondary accession number(s): Q54NC7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3