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Protein

Dual specificity protein kinase splB

Gene

splB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei880ATPPROSITE-ProRule annotation1
Active sitei977Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi859 – 867ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • hyperosmotic response Source: dictyBase
  • positive regulation of gene expression Source: dictyBase
  • positive regulation of protein import into nucleus Source: dictyBase
  • positive regulation of STAT cascade Source: dictyBase
  • protein autophosphorylation Source: dictyBase
  • response to differentiation-inducing factor 1 Source: dictyBase
  • sorocarp development Source: dictyBase
  • tyrosine phosphorylation of STAT protein Source: dictyBase

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase splB (EC:2.7.10.2)
Alternative name(s):
Non-receptor tyrosine kinase spore lysis B
Tyrosine-protein kinase 2
Gene namesi
Name:splB
Synonyms:DpyK2, pyk2, pykB
ORF Names:DDB_G0285321
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285321 splB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000881222 – 1155Dual specificity protein kinase splBAdd BLAST1154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP18161
PRIDEiP18161

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0191483

Structurei

3D structure databases

ProteinModelPortaliP18161
SMRiP18161
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini853 – 1126Protein kinasePROSITE-ProRule annotationAdd BLAST274

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi103 – 124Gln-richAdd BLAST22
Compositional biasi142 – 746Asn-richAdd BLAST605
Compositional biasi709 – 758Thr-richAdd BLAST50

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0192 Eukaryota
COG0515 LUCA
InParanoidiP18161
OMAiASIVCEN

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008266 Tyr_kinase_AS
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGITSSKPVS GSNTTTNSPT NQKAISEKKQ KPKRTNKRNL STDSKSSTCS
60 70 80 90 100
SPTNNYLADE LVVDLNSMEI EGADSIQYSG HSHSSSMTNS MTNSMRNSAN
110 120 130 140 150
EKQLQQKQQQ PQQQPQQQQP QPHQSHDDLN SSALDQDDGE FNNNFENESE
160 170 180 190 200
VESISSSSIN NNNTNNNSNN NNNNNNNNNN NNNNNNNIPS ISNISNNNIN
210 220 230 240 250
NNINNNNNNN SCKQKNSKNK KNKNKNNNNK NSSSSNERPY SISYDNEGST
260 270 280 290 300
TKSNSTLSLY SNISNLHSIT SHSIQDASIV CENCNHHMTK PIHLLSLSLE
310 320 330 340 350
PTSAASLDIH RNGNFSITLA SNSRSSSPAT ILQQNNKHFL INSNINIINT
360 370 380 390 400
SLNNNNKNKD EDNVNNNNGN NSNNNNNINN NNDEDNDEDN DENNNNNNNN
410 420 430 440 450
NNNNNNNNNN NNNNTSNNDI PDLSWTNYNS DSFNNNNNNN NIFNTFLSIS
460 470 480 490 500
SPISDQQNTS PQIQEIISPL PSPPTVQQSD VIQQTTTTSP SPLPSPQKQK
510 520 530 540 550
QQQQRPLQKS SPPPTTITTS TTTTTQFNNN NLASSLGSTS DLFNKSLISS
560 570 580 590 600
FLNGQGISSI NNSNPFENSY KNQINSSTNS GTNTFSNTNT NINTTNSSNT
610 620 630 640 650
NVGYDQYSFN YSNGSNSNIN NSNNGLKSFL SNSNSSFSAL KNCSYDAKDM
660 670 680 690 700
SSFTITLSNS ELNTMIAESL NRSSASNSIV KSSNTVHTTS GGNNSLCSDG
710 720 730 740 750
YNQNIISATT NTNTTTTNSS STTTTTTATT TTASTTNNNI NNNNNNSTST
760 770 780 790 800
TNSTKDITFL VCDNPDSTKE KSNVSNTSSI ISASNLNRHI TPNSHMRPRG
810 820 830 840 850
RSISESLIMS PINKESLNDI QRAIESEKIK KTKFEELKSI LGEREYIIDI
860 870 880 890 900
NDIQFIQKVG EGAFSEVWEG WWKGIHVAIK KLKIIGDEEQ FKERFIREVQ
910 920 930 940 950
NLKKGNHQNI VMFIGACYKP ACIITEYMAG GSLYNILHNP NSSTPKVKYS
960 970 980 990 1000
FPLVLKMATD MALGLLHLHS ITIVHRDLTS QNILLDELGN IKISDFGLSR
1010 1020 1030 1040 1050
EKSREGSMTM TNGGICNPRW RPPELTKNLG HYSEKVDVYC FSLVVWEILT
1060 1070 1080 1090 1100
GEIPFSDLDG SQASAQVAYA GLRPPIPEYC DPELKLLLTQ CWEADPNDRP
1110 1120 1130 1140 1150
PFTYIVNKLK EISWNNPIGF VSDQFYQYSE PSTPRLALSN QSSNSSSISL

SPTKL
Length:1,155
Mass (Da):127,437
Last modified:December 4, 2007 - v2
Checksum:i2A00D04A39262BC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti746 – 749NSTS → RFYN in AAA33203 (PubMed:1972546).Curated4
Sequence conflicti1000R → A in AAA33203 (PubMed:1972546).Curated1
Sequence conflicti1063A → R in AAA33203 (PubMed:1972546).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000079 Genomic DNA Translation: EAL64735.1
M33784 mRNA Translation: AAA33203.1
PIRiB35670
RefSeqiXP_638253.1, XM_633161.1

Genome annotation databases

EnsemblProtistsiEAL64735; EAL64735; DDB_G0285321
GeneIDi8625061
KEGGiddi:DDB_G0285321

Entry informationi

Entry nameiSPLB_DICDI
AccessioniPrimary (citable) accession number: P18161
Secondary accession number(s): Q54NC7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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