P18161 (SPLB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein kinase splB
EC=2.7.10.2

Alternative name(s):
Non-receptor tyrosine kinase spore lysis B
Tyrosine-protein kinase 2

Gene names

Name:	splB
Synonyms:	DpyK2, pyk2, pykB
ORF Names:	DDB_G0285321

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Tyr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from direct assay Ref.2. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 1155	1154	Dual specificity protein kinase splB		PRO_0000088122
Regions
	Domain	853 – 1126	274	Protein kinase
	Nucleotide binding	859 – 867	9	ATP By similarity
	Compositional bias	103 – 124	22	Gln-rich
	Compositional bias	142 – 746	605	Asn-rich
	Compositional bias	709 – 758	50	Thr-rich
Sites
	Active site	977	1	Proton acceptor By similarity
	Binding site	880	1	ATP By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
Experimental info
	Sequence conflict	746 – 749	4	NSTS → RFYN in AAA33203. Ref.2
	Sequence conflict	1000	1	R → A in AAA33203. Ref.2
	Sequence conflict	1063	1	A → R in AAA33203. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18161 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 2A00D04A39262BC2	FASTA	1,155	127,437
	10 20 30 40 50 60 MGITSSKPVS GSNTTTNSPT NQKAISEKKQ KPKRTNKRNL STDSKSSTCS SPTNNYLADE 70 80 90 100 110 120 LVVDLNSMEI EGADSIQYSG HSHSSSMTNS MTNSMRNSAN EKQLQQKQQQ PQQQPQQQQP 130 140 150 160 170 180 QPHQSHDDLN SSALDQDDGE FNNNFENESE VESISSSSIN NNNTNNNSNN NNNNNNNNNN 190 200 210 220 230 240 NNNNNNNIPS ISNISNNNIN NNINNNNNNN SCKQKNSKNK KNKNKNNNNK NSSSSNERPY 250 260 270 280 290 300 SISYDNEGST TKSNSTLSLY SNISNLHSIT SHSIQDASIV CENCNHHMTK PIHLLSLSLE 310 320 330 340 350 360 PTSAASLDIH RNGNFSITLA SNSRSSSPAT ILQQNNKHFL INSNINIINT SLNNNNKNKD 370 380 390 400 410 420 EDNVNNNNGN NSNNNNNINN NNDEDNDEDN DENNNNNNNN NNNNNNNNNN NNNNTSNNDI 430 440 450 460 470 480 PDLSWTNYNS DSFNNNNNNN NIFNTFLSIS SPISDQQNTS PQIQEIISPL PSPPTVQQSD 490 500 510 520 530 540 VIQQTTTTSP SPLPSPQKQK QQQQRPLQKS SPPPTTITTS TTTTTQFNNN NLASSLGSTS 550 560 570 580 590 600 DLFNKSLISS FLNGQGISSI NNSNPFENSY KNQINSSTNS GTNTFSNTNT NINTTNSSNT 610 620 630 640 650 660 NVGYDQYSFN YSNGSNSNIN NSNNGLKSFL SNSNSSFSAL KNCSYDAKDM SSFTITLSNS 670 680 690 700 710 720 ELNTMIAESL NRSSASNSIV KSSNTVHTTS GGNNSLCSDG YNQNIISATT NTNTTTTNSS 730 740 750 760 770 780 STTTTTTATT TTASTTNNNI NNNNNNSTST TNSTKDITFL VCDNPDSTKE KSNVSNTSSI 790 800 810 820 830 840 ISASNLNRHI TPNSHMRPRG RSISESLIMS PINKESLNDI QRAIESEKIK KTKFEELKSI 850 860 870 880 890 900 LGEREYIIDI NDIQFIQKVG EGAFSEVWEG WWKGIHVAIK KLKIIGDEEQ FKERFIREVQ 910 920 930 940 950 960 NLKKGNHQNI VMFIGACYKP ACIITEYMAG GSLYNILHNP NSSTPKVKYS FPLVLKMATD 970 980 990 1000 1010 1020 MALGLLHLHS ITIVHRDLTS QNILLDELGN IKISDFGLSR EKSREGSMTM TNGGICNPRW 1030 1040 1050 1060 1070 1080 RPPELTKNLG HYSEKVDVYC FSLVVWEILT GEIPFSDLDG SQASAQVAYA GLRPPIPEYC 1090 1100 1110 1120 1130 1140 DPELKLLLTQ CWEADPNDRP PFTYIVNKLK EISWNNPIGF VSDQFYQYSE PSTPRLALSN 1150 QSSNSSSISL SPTKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum." Tan J.L., Spudich J.A. Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 746-1155.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000079 Genomic DNA. Translation: EAL64735.1. M33784 mRNA. Translation: AAA33203.1.
PIR	B35670.
RefSeq	XP_638253.1. XM_633161.1.
3D structure databases
ProteinModelPortal	P18161.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191483.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191483; DDB0191483; DDB_G0285321.
GeneID	8625061.
KEGG	ddi:DDB_G0285321.
Organism-specific databases
dictyBase	DDB_G0285321. splB.
Phylogenomic databases
eggNOG	COG0515.
OMA	ASIVCEN.
ProtClustDB	CLSZ2846645.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPLB_DICDI

Accession

Primary (citable) accession number: P18161
Secondary accession number(s): Q54NC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)