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P18161

- SPLB_DICDI

UniProt

P18161 - SPLB_DICDI

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Protein

Dual specificity protein kinase splB

Gene

splB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei880 – 8801ATPPROSITE-ProRule annotation
Active sitei977 – 9771Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi859 – 8679ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein tyrosine kinase activity Source: dictyBase

GO - Biological processi

  1. positive regulation of STAT protein import into nucleus Source: dictyBase
  2. tyrosine phosphorylation of STAT protein Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase splB (EC:2.7.10.2)
Alternative name(s):
Non-receptor tyrosine kinase spore lysis B
Tyrosine-protein kinase 2
Gene namesi
Name:splB
Synonyms:DpyK2, pyk2, pykB
ORF Names:DDB_G0285321
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285321. splB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 11551154Dual specificity protein kinase splBPRO_0000088122Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence Analysis

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0191483.

Structurei

3D structure databases

ProteinModelPortaliP18161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini853 – 1126274Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi103 – 12422Gln-richAdd
BLAST
Compositional biasi142 – 746605Asn-richAdd
BLAST
Compositional biasi709 – 75850Thr-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP18161.
OMAiASIVCEN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18161-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGITSSKPVS GSNTTTNSPT NQKAISEKKQ KPKRTNKRNL STDSKSSTCS
60 70 80 90 100
SPTNNYLADE LVVDLNSMEI EGADSIQYSG HSHSSSMTNS MTNSMRNSAN
110 120 130 140 150
EKQLQQKQQQ PQQQPQQQQP QPHQSHDDLN SSALDQDDGE FNNNFENESE
160 170 180 190 200
VESISSSSIN NNNTNNNSNN NNNNNNNNNN NNNNNNNIPS ISNISNNNIN
210 220 230 240 250
NNINNNNNNN SCKQKNSKNK KNKNKNNNNK NSSSSNERPY SISYDNEGST
260 270 280 290 300
TKSNSTLSLY SNISNLHSIT SHSIQDASIV CENCNHHMTK PIHLLSLSLE
310 320 330 340 350
PTSAASLDIH RNGNFSITLA SNSRSSSPAT ILQQNNKHFL INSNINIINT
360 370 380 390 400
SLNNNNKNKD EDNVNNNNGN NSNNNNNINN NNDEDNDEDN DENNNNNNNN
410 420 430 440 450
NNNNNNNNNN NNNNTSNNDI PDLSWTNYNS DSFNNNNNNN NIFNTFLSIS
460 470 480 490 500
SPISDQQNTS PQIQEIISPL PSPPTVQQSD VIQQTTTTSP SPLPSPQKQK
510 520 530 540 550
QQQQRPLQKS SPPPTTITTS TTTTTQFNNN NLASSLGSTS DLFNKSLISS
560 570 580 590 600
FLNGQGISSI NNSNPFENSY KNQINSSTNS GTNTFSNTNT NINTTNSSNT
610 620 630 640 650
NVGYDQYSFN YSNGSNSNIN NSNNGLKSFL SNSNSSFSAL KNCSYDAKDM
660 670 680 690 700
SSFTITLSNS ELNTMIAESL NRSSASNSIV KSSNTVHTTS GGNNSLCSDG
710 720 730 740 750
YNQNIISATT NTNTTTTNSS STTTTTTATT TTASTTNNNI NNNNNNSTST
760 770 780 790 800
TNSTKDITFL VCDNPDSTKE KSNVSNTSSI ISASNLNRHI TPNSHMRPRG
810 820 830 840 850
RSISESLIMS PINKESLNDI QRAIESEKIK KTKFEELKSI LGEREYIIDI
860 870 880 890 900
NDIQFIQKVG EGAFSEVWEG WWKGIHVAIK KLKIIGDEEQ FKERFIREVQ
910 920 930 940 950
NLKKGNHQNI VMFIGACYKP ACIITEYMAG GSLYNILHNP NSSTPKVKYS
960 970 980 990 1000
FPLVLKMATD MALGLLHLHS ITIVHRDLTS QNILLDELGN IKISDFGLSR
1010 1020 1030 1040 1050
EKSREGSMTM TNGGICNPRW RPPELTKNLG HYSEKVDVYC FSLVVWEILT
1060 1070 1080 1090 1100
GEIPFSDLDG SQASAQVAYA GLRPPIPEYC DPELKLLLTQ CWEADPNDRP
1110 1120 1130 1140 1150
PFTYIVNKLK EISWNNPIGF VSDQFYQYSE PSTPRLALSN QSSNSSSISL

SPTKL
Length:1,155
Mass (Da):127,437
Last modified:December 4, 2007 - v2
Checksum:i2A00D04A39262BC2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti746 – 7494NSTS → RFYN in AAA33203. (PubMed:1972546)Curated
Sequence conflicti1000 – 10001R → A in AAA33203. (PubMed:1972546)Curated
Sequence conflicti1063 – 10631A → R in AAA33203. (PubMed:1972546)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000079 Genomic DNA. Translation: EAL64735.1.
M33784 mRNA. Translation: AAA33203.1.
PIRiB35670.
RefSeqiXP_638253.1. XM_633161.1.

Genome annotation databases

EnsemblProtistsiDDB0191483; DDB0191483; DDB_G0285321.
GeneIDi8625061.
KEGGiddi:DDB_G0285321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000079 Genomic DNA. Translation: EAL64735.1 .
M33784 mRNA. Translation: AAA33203.1 .
PIRi B35670.
RefSeqi XP_638253.1. XM_633161.1.

3D structure databases

ProteinModelPortali P18161.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191483.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191483 ; DDB0191483 ; DDB_G0285321 .
GeneIDi 8625061.
KEGGi ddi:DDB_G0285321.

Organism-specific databases

dictyBasei DDB_G0285321. splB.

Phylogenomic databases

eggNOGi COG0515.
InParanoidi P18161.
OMAi ASIVCEN.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
[Graphical view ]
PRINTSi PR00109. TYRKINASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum."
    Tan J.L., Spudich J.A.
    Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 746-1155.

Entry informationi

Entry nameiSPLB_DICDI
AccessioniPrimary (citable) accession number: P18161
Secondary accession number(s): Q54NC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3