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P18160

- SPLA_DICDI

UniProt

P18160 - SPLA_DICDI

Protein

Dual specificity protein kinase splA

Gene

splA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 3 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Essential for spore differentiation.

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei2142 – 21421ATPPROSITE-ProRule annotation
    Active sitei2243 – 22431Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi2121 – 21299ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein serine/threonine/tyrosine kinase activity Source: dictyBase
    4. protein tyrosine kinase activity Source: dictyBase

    GO - Biological processi

    1. anatomical structure morphogenesis Source: dictyBase
    2. peptidyl-tyrosine phosphorylation Source: GOC
    3. sorocarp development Source: dictyBase
    4. sporulation resulting in formation of a cellular spore Source: dictyBase

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.12.1. 1939.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein kinase splA (EC:2.7.10.2)
    Alternative name(s):
    Non-receptor tyrosine kinase spore lysis A
    Tyrosine-protein kinase 1
    Gene namesi
    Name:splA
    Synonyms:DpyK1, pyK1, pykA
    ORF Names:DDB_G0283385
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0283385. splA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 24102410Dual specificity protein kinase splAPRO_0000088121Add
    BLAST

    Post-translational modificationi

    Tyrosine kinase domain is capable of autophosphorylation, in vitro; however it is also autophosphorylated on serine and threonine residues.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP18160.

    Expressioni

    Developmental stagei

    Expressed in vegetative cells and throughout development with a peak during the mound stage of morphogenesis.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0215670.

    Structurei

    3D structure databases

    ProteinModelPortaliP18160.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini822 – 1004183B30.2/SPRY 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1020 – 1209190B30.2/SPRY 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1481 – 1703223B30.2/SPRY 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini1734 – 179865SAMPROSITE-ProRule annotationAdd
    BLAST
    Domaini2115 – 2387273Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi23 – 5230Poly-AsnAdd
    BLAST
    Compositional biasi162 – 320159Ser-richAdd
    BLAST
    Compositional biasi213 – 2197Poly-Asn
    Compositional biasi235 – 24511Poly-AsnAdd
    BLAST
    Compositional biasi321 – 35232Poly-GlnAdd
    BLAST
    Compositional biasi447 – 51468Poly-GlnAdd
    BLAST
    Compositional biasi515 – 648134Pro-richAdd
    BLAST
    Compositional biasi601 – 61010Poly-Asn
    Compositional biasi631 – 759129Ser-richAdd
    BLAST
    Compositional biasi706 – 72015Poly-GlnAdd
    BLAST
    Compositional biasi777 – 81337Poly-AsnAdd
    BLAST
    Compositional biasi1229 – 124618Poly-AsnAdd
    BLAST
    Compositional biasi1275 – 130632Poly-AsnAdd
    BLAST
    Compositional biasi1309 – 13179Poly-Asn
    Compositional biasi1320 – 135839Poly-AsnAdd
    BLAST
    Compositional biasi1422 – 14265Poly-Asn
    Compositional biasi1852 – 18554Poly-Ser
    Compositional biasi2021 – 203616Poly-AsnAdd
    BLAST
    Compositional biasi2041 – 20466Poly-Gln
    Compositional biasi2050 – 205910Poly-Gln
    Compositional biasi2088 – 210316Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 3 B30.2/SPRY domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    OMAiRWIVEDE.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    InterProiIPR001870. B30.2/SPRY.
    IPR008985. ConA-like_lec_gl_sf.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR018355. SPla/RYanodine_receptor_subgr.
    IPR003877. SPRY_rcpt.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF07647. SAM_2. 1 hit.
    PF00622. SPRY. 3 hits.
    [Graphical view]
    SMARTiSM00454. SAM. 1 hit.
    SM00449. SPRY. 3 hits.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF49899. SSF49899. 3 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50188. B302_SPRY. 3 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18160-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSKNDLFIG FFFFFYNYYY YYNNNNNNNN NNNNNNNNNN NNNNNNNNNN     50
    NNIYIIVIIG LDPQQNHPSL KIPPPPSPTS PYVRRHARQH SRSNSSNSPG 100
    ELTGGVEIIQ QQNSINTQTS PPTSTSPNTV PPPPPTNTTT SSTTITRNSN 150
    NINNSNGGII NSSSGSNINN SSSSGVINTN INNSGGNISP TSNSLPSSNN 200
    NILYTSSGSN SGNNNNNNNT INISSGSSGI NNSSNSNINN NNNNNSSSSS 250
    GIHASGSLTA IPTNTNSNSS IFHSSNSVNR INKSNYSADS LVLPPNRISQ 300
    VPQSQTQPQL QISPSTSFSS QQQQQQQLQQ QLQLQQQLQQ QIQQQQQIQQ 350
    QQNLHFTYSK TFTSGQPNTL VNLSSPPPQS KHLHVSSDHS FFNEVLTPTP 400
    VIHNSTNQNN QLLFGDSDPL SFLEYNNFIN RYQPALKNNQ PVSSYRQQQQ 450
    IQHQIQLQQI QQQQQQQQQI QQQQLQQQQQ IQQQQIQQQQ QQIQQQQQQQ 500
    QQQQQQQQQL QQIQPPPTIQ PPPQQTTPTL RGNRSSGNLS GLNSFSLKQS 550
    TDSLTPPPNS QQSTVSSNST PIAATPISPL TAPTSPPPPP PPPTNFNSKF 600
    NNNNNNNINN SSNNNTTVPP SPPPIIVLPK SPSSRSPRPA SAPVPSAPFL 650
    VNNRVINTSS SSNISTNNTD LNLSSSSSSS PPLNISTASP SKSEDSPPTV 700
    SPSYKQQQQQ QQQQQQQQQQ LNNSSNSSYS PKPRSPSVSS PPPSSISPNS 750
    SPIGSPNISF HHHQQHQIPP PPPVLSNTNN NNNNNNNNNN NNNNNNNNNN 800
    NNNNNNNNNN NTNHTNKKEG DSSWFNMSFK FFKKKLVPSN EYRWDLRKSN 850
    SLTLNIEDKS RCSYRLPTSG SKGIAKSTQP FSSSFTYFEL FITNGNGDKI 900
    CFGLTTNDHP IEVYPGNYQG SYGYSGDGKC YFGTNEGRVY GPSFSSGDVV 950
    GCGYDSSSKT LYFTKNGVYL GVAAQKVNLI GLYPTVGLQN PGESVVINFF 1000
    GPFSYRGAPE KPSKQSTIKD SGGSSIIPSE DLIPKEEFEV CRWSEKKNYH 1050
    GKHVVVRNRT AFLPLDSPKD TIGGVRATQP FGEGFCYFEV IIDQLDKGQL 1100
    SIGLANLEYP TFYHVGWMPR SYGYHNDDGR KFRWREEPGV NEGESYGSSY 1150
    KKGDIIGCGL SFTSREIFFT KNGMYLGTAF SNVYGVFYPS VAFNEPGISI 1200
    TGVFGPPFKF SQVTLMLKNV NSTSILVPNG NNNNNSNNNN NNNNNNIIGN 1250
    GKITTTTTTS TSPSSINNNE DISSNNNNNN NNNNNNNNNN NNNNNNNNNN 1300
    NNNNSNSSNT NNNNINNTTN NNNSNSNNNN NNNNSNSNSN SNNNNINNNN 1350
    NNNNNNNNIY LTKKPSIGST DESSTGSLGG NNSSGNNNSS SGSIGNNSSI 1400
    IKQRSPPHSI NGPLMLPPSS TNNNNNIYSS YNSTTAGSST TILPTLNHPI 1450
    FGNTTSNNNS SSTLSVGGNN NLLGRHCQSL PITASTNHTL SSSLGVSFSS 1500
    PSSSPKTSPR KIVNSSEDLG FVQTFQDQDG QPPSAWRRCG KSIKTKDDIT 1550
    LTIIKKKTSV AMADRPFSSN SSSTICYFEV YLEGHDKKGS ITVGLSHSTY 1600
    PFIKHIGREP KSYGFSSEGE KYGGSEIGEP YGPFFFFDGD SIASSCVIGC 1650
    GINTSTRDIF FTKNGHYLGV AFSRVTSDPL YPSISFRGVV GGLCVATFPG 1700
    GHFRFNIEDL PGISPSVWTE ALGPDRQGSG FKNWAPNDVA IWLESFNYGQ 1750
    YRKNFRDNNI SGRHLEGITH AMLKNDLGIE PYGHREDIIN RLNRMIQIWN 1800
    DKSPDSYPKI AIDSSDKIRW PASGGSSGGI NISGGVVIGS SSGSDDGITE 1850
    ISSSSKNIRP YKSYTQKEIE DRNRRSTISG GEKKNKYYID NQMDPHQIGS 1900
    MDSDGLLPDF GQGPPDEKNS SKTLSNEQIR YLQQRKDEPP IAISSTGNGG 1950
    SVSSTGGSSG FLTFPSSNSL THPPQRDKPT QEFTHLPPIT SNYKGITNTG 2000
    QPHKSFDQPL ELFPRHSAFS NNGNNGNNNN NNNNNNIKAN QQQQQQSSYQ 2050
    QSQTQQQQQH ITSTSTSTTN KWIDPFGGWE TQSSLSHPPS RPPPPPPPPP 2100
    QLPVRSEYEI DFNELEFGQT IGKGFFGEVK RGYWRETDVA IKIIYRDQFK 2150
    TKSSLVMFQN EVGILSKLRH PNVVQFLGAC TAGGEDHHCI VTEWMGGGSL 2200
    RQFLTDHFNL LEQNPHIRLK LALDIAKGMN YLHGWTPPIL HRDLSSRNIL 2250
    LDHNIDPKNP VVSSRQDIKC KISDFGLSRL KMEQASQMTQ SVGCIPYMAP 2300
    EVFKGDSNSE KSDVYSYGMV LFELLTSDEP QQDMKPMKMA HLAAYESYRP 2350
    PIPLTTSSKW KEILTQCWDS NPDSRPTFKQ IIVHLKEMED QGVSSFASVP 2400
    VQTIDTGVYA 2410
    Length:2,410
    Mass (Da):264,919
    Last modified:December 4, 2007 - v3
    Checksum:i768EC59E9E05C229
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2074 – 20741D → R in AAA33202. (PubMed:1972546)Curated
    Sequence conflicti2261 – 22611V → L in AAA33202. (PubMed:1972546)Curated
    Sequence conflicti2282 – 22821M → K in AAB41125. (PubMed:8898241)Curated
    Sequence conflicti2282 – 22821M → K in AAA33202. (PubMed:1972546)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000055 Genomic DNA. Translation: EAL65677.1.
    U32174 Genomic DNA. Translation: AAB41125.1.
    M33785 mRNA. Translation: AAA33202.1.
    PIRiT18276.
    RefSeqiXP_639040.1. XM_633948.1.

    Genome annotation databases

    EnsemblProtistsiDDB0252636; DDB0252636; DDB_G0283385.
    GeneIDi8624065.
    KEGGiddi:DDB_G0283385.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000055 Genomic DNA. Translation: EAL65677.1 .
    U32174 Genomic DNA. Translation: AAB41125.1 .
    M33785 mRNA. Translation: AAA33202.1 .
    PIRi T18276.
    RefSeqi XP_639040.1. XM_633948.1.

    3D structure databases

    ProteinModelPortali P18160.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0215670.

    Proteomic databases

    PRIDEi P18160.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0252636 ; DDB0252636 ; DDB_G0283385 .
    GeneIDi 8624065.
    KEGGi ddi:DDB_G0283385.

    Organism-specific databases

    dictyBasei DDB_G0283385. splA.

    Phylogenomic databases

    eggNOGi COG0515.
    OMAi RWIVEDE.

    Enzyme and pathway databases

    BRENDAi 2.7.12.1. 1939.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    InterProi IPR001870. B30.2/SPRY.
    IPR008985. ConA-like_lec_gl_sf.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR018355. SPla/RYanodine_receptor_subgr.
    IPR003877. SPRY_rcpt.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF07647. SAM_2. 1 hit.
    PF00622. SPRY. 3 hits.
    [Graphical view ]
    SMARTi SM00454. SAM. 1 hit.
    SM00449. SPRY. 3 hits.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF49899. SSF49899. 3 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50188. B302_SPRY. 3 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The Dictyostelium dual-specificity kinase splA is essential for spore differentiation."
      Nuckolls G.H., Osherov N., Loomis W.F., Spudich J.A.
      Development 122:3295-3305(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 827-2410, AUTOPHOSPHORYLATION, DEVELOPMENTAL STAGE.
      Strain: JH10.
    3. "Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum."
      Tan J.L., Spudich J.A.
      Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2074-2410.

    Entry informationi

    Entry nameiSPLA_DICDI
    AccessioniPrimary (citable) accession number: P18160
    Secondary accession number(s): Q54R51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 118 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3