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Protein

Dual specificity protein kinase splA

Gene

splA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for spore differentiation.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei2142ATPPROSITE-ProRule annotation1
Active sitei2243Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi2121 – 2129ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • anatomical structure morphogenesis Source: dictyBase
  • intracellular signal transduction Source: GO_Central
  • sorocarp development Source: dictyBase
  • sporulation resulting in formation of a cellular spore Source: dictyBase

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1 1939

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase splA (EC:2.7.10.2)
Alternative name(s):
Non-receptor tyrosine kinase spore lysis A
Tyrosine-protein kinase 1
Gene namesi
Name:splA
Synonyms:DpyK1, pyK1, pykA
ORF Names:DDB_G0283385
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283385 splA

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881211 – 2410Dual specificity protein kinase splAAdd BLAST2410

Post-translational modificationi

Tyrosine kinase domain is capable of autophosphorylation, in vitro; however it is also autophosphorylated on serine and threonine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP18160
PRIDEiP18160

Expressioni

Developmental stagei

Expressed in vegetative cells and throughout development with a peak during the mound stage of morphogenesis.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0252636

Structurei

3D structure databases

ProteinModelPortaliP18160
SMRiP18160
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini822 – 1004B30.2/SPRY 1PROSITE-ProRule annotationAdd BLAST183
Domaini1020 – 1209B30.2/SPRY 2PROSITE-ProRule annotationAdd BLAST190
Domaini1481 – 1703B30.2/SPRY 3PROSITE-ProRule annotationAdd BLAST223
Domaini1734 – 1798SAMPROSITE-ProRule annotationAdd BLAST65
Domaini2115 – 2387Protein kinasePROSITE-ProRule annotationAdd BLAST273

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 52Poly-AsnAdd BLAST30
Compositional biasi162 – 320Ser-richAdd BLAST159
Compositional biasi213 – 219Poly-Asn7
Compositional biasi235 – 245Poly-AsnAdd BLAST11
Compositional biasi321 – 352Poly-GlnAdd BLAST32
Compositional biasi447 – 514Poly-GlnAdd BLAST68
Compositional biasi515 – 648Pro-richAdd BLAST134
Compositional biasi601 – 610Poly-Asn10
Compositional biasi631 – 759Ser-richAdd BLAST129
Compositional biasi706 – 720Poly-GlnAdd BLAST15
Compositional biasi777 – 813Poly-AsnAdd BLAST37
Compositional biasi1229 – 1246Poly-AsnAdd BLAST18
Compositional biasi1275 – 1306Poly-AsnAdd BLAST32
Compositional biasi1309 – 1317Poly-Asn9
Compositional biasi1320 – 1358Poly-AsnAdd BLAST39
Compositional biasi1422 – 1426Poly-Asn5
Compositional biasi1852 – 1855Poly-Ser4
Compositional biasi2021 – 2036Poly-AsnAdd BLAST16
Compositional biasi2041 – 2046Poly-Gln6
Compositional biasi2050 – 2059Poly-Gln10
Compositional biasi2088 – 2103Pro-richAdd BLAST16

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0192 Eukaryota
COG0515 LUCA
InParanoidiP18160
OMAiFRDNNIS

Family and domain databases

InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR003877 SPRY_dom
IPR008266 Tyr_kinase_AS
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PF07647 SAM_2, 1 hit
PF00622 SPRY, 3 hits
SMARTiView protein in SMART
SM00454 SAM, 1 hit
SM00449 SPRY, 3 hits
SUPFAMiSSF47769 SSF47769, 1 hit
SSF49899 SSF49899, 3 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 3 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

P18160-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSKNDLFIG FFFFFYNYYY YYNNNNNNNN NNNNNNNNNN NNNNNNNNNN
60 70 80 90 100
NNIYIIVIIG LDPQQNHPSL KIPPPPSPTS PYVRRHARQH SRSNSSNSPG
110 120 130 140 150
ELTGGVEIIQ QQNSINTQTS PPTSTSPNTV PPPPPTNTTT SSTTITRNSN
160 170 180 190 200
NINNSNGGII NSSSGSNINN SSSSGVINTN INNSGGNISP TSNSLPSSNN
210 220 230 240 250
NILYTSSGSN SGNNNNNNNT INISSGSSGI NNSSNSNINN NNNNNSSSSS
260 270 280 290 300
GIHASGSLTA IPTNTNSNSS IFHSSNSVNR INKSNYSADS LVLPPNRISQ
310 320 330 340 350
VPQSQTQPQL QISPSTSFSS QQQQQQQLQQ QLQLQQQLQQ QIQQQQQIQQ
360 370 380 390 400
QQNLHFTYSK TFTSGQPNTL VNLSSPPPQS KHLHVSSDHS FFNEVLTPTP
410 420 430 440 450
VIHNSTNQNN QLLFGDSDPL SFLEYNNFIN RYQPALKNNQ PVSSYRQQQQ
460 470 480 490 500
IQHQIQLQQI QQQQQQQQQI QQQQLQQQQQ IQQQQIQQQQ QQIQQQQQQQ
510 520 530 540 550
QQQQQQQQQL QQIQPPPTIQ PPPQQTTPTL RGNRSSGNLS GLNSFSLKQS
560 570 580 590 600
TDSLTPPPNS QQSTVSSNST PIAATPISPL TAPTSPPPPP PPPTNFNSKF
610 620 630 640 650
NNNNNNNINN SSNNNTTVPP SPPPIIVLPK SPSSRSPRPA SAPVPSAPFL
660 670 680 690 700
VNNRVINTSS SSNISTNNTD LNLSSSSSSS PPLNISTASP SKSEDSPPTV
710 720 730 740 750
SPSYKQQQQQ QQQQQQQQQQ LNNSSNSSYS PKPRSPSVSS PPPSSISPNS
760 770 780 790 800
SPIGSPNISF HHHQQHQIPP PPPVLSNTNN NNNNNNNNNN NNNNNNNNNN
810 820 830 840 850
NNNNNNNNNN NTNHTNKKEG DSSWFNMSFK FFKKKLVPSN EYRWDLRKSN
860 870 880 890 900
SLTLNIEDKS RCSYRLPTSG SKGIAKSTQP FSSSFTYFEL FITNGNGDKI
910 920 930 940 950
CFGLTTNDHP IEVYPGNYQG SYGYSGDGKC YFGTNEGRVY GPSFSSGDVV
960 970 980 990 1000
GCGYDSSSKT LYFTKNGVYL GVAAQKVNLI GLYPTVGLQN PGESVVINFF
1010 1020 1030 1040 1050
GPFSYRGAPE KPSKQSTIKD SGGSSIIPSE DLIPKEEFEV CRWSEKKNYH
1060 1070 1080 1090 1100
GKHVVVRNRT AFLPLDSPKD TIGGVRATQP FGEGFCYFEV IIDQLDKGQL
1110 1120 1130 1140 1150
SIGLANLEYP TFYHVGWMPR SYGYHNDDGR KFRWREEPGV NEGESYGSSY
1160 1170 1180 1190 1200
KKGDIIGCGL SFTSREIFFT KNGMYLGTAF SNVYGVFYPS VAFNEPGISI
1210 1220 1230 1240 1250
TGVFGPPFKF SQVTLMLKNV NSTSILVPNG NNNNNSNNNN NNNNNNIIGN
1260 1270 1280 1290 1300
GKITTTTTTS TSPSSINNNE DISSNNNNNN NNNNNNNNNN NNNNNNNNNN
1310 1320 1330 1340 1350
NNNNSNSSNT NNNNINNTTN NNNSNSNNNN NNNNSNSNSN SNNNNINNNN
1360 1370 1380 1390 1400
NNNNNNNNIY LTKKPSIGST DESSTGSLGG NNSSGNNNSS SGSIGNNSSI
1410 1420 1430 1440 1450
IKQRSPPHSI NGPLMLPPSS TNNNNNIYSS YNSTTAGSST TILPTLNHPI
1460 1470 1480 1490 1500
FGNTTSNNNS SSTLSVGGNN NLLGRHCQSL PITASTNHTL SSSLGVSFSS
1510 1520 1530 1540 1550
PSSSPKTSPR KIVNSSEDLG FVQTFQDQDG QPPSAWRRCG KSIKTKDDIT
1560 1570 1580 1590 1600
LTIIKKKTSV AMADRPFSSN SSSTICYFEV YLEGHDKKGS ITVGLSHSTY
1610 1620 1630 1640 1650
PFIKHIGREP KSYGFSSEGE KYGGSEIGEP YGPFFFFDGD SIASSCVIGC
1660 1670 1680 1690 1700
GINTSTRDIF FTKNGHYLGV AFSRVTSDPL YPSISFRGVV GGLCVATFPG
1710 1720 1730 1740 1750
GHFRFNIEDL PGISPSVWTE ALGPDRQGSG FKNWAPNDVA IWLESFNYGQ
1760 1770 1780 1790 1800
YRKNFRDNNI SGRHLEGITH AMLKNDLGIE PYGHREDIIN RLNRMIQIWN
1810 1820 1830 1840 1850
DKSPDSYPKI AIDSSDKIRW PASGGSSGGI NISGGVVIGS SSGSDDGITE
1860 1870 1880 1890 1900
ISSSSKNIRP YKSYTQKEIE DRNRRSTISG GEKKNKYYID NQMDPHQIGS
1910 1920 1930 1940 1950
MDSDGLLPDF GQGPPDEKNS SKTLSNEQIR YLQQRKDEPP IAISSTGNGG
1960 1970 1980 1990 2000
SVSSTGGSSG FLTFPSSNSL THPPQRDKPT QEFTHLPPIT SNYKGITNTG
2010 2020 2030 2040 2050
QPHKSFDQPL ELFPRHSAFS NNGNNGNNNN NNNNNNIKAN QQQQQQSSYQ
2060 2070 2080 2090 2100
QSQTQQQQQH ITSTSTSTTN KWIDPFGGWE TQSSLSHPPS RPPPPPPPPP
2110 2120 2130 2140 2150
QLPVRSEYEI DFNELEFGQT IGKGFFGEVK RGYWRETDVA IKIIYRDQFK
2160 2170 2180 2190 2200
TKSSLVMFQN EVGILSKLRH PNVVQFLGAC TAGGEDHHCI VTEWMGGGSL
2210 2220 2230 2240 2250
RQFLTDHFNL LEQNPHIRLK LALDIAKGMN YLHGWTPPIL HRDLSSRNIL
2260 2270 2280 2290 2300
LDHNIDPKNP VVSSRQDIKC KISDFGLSRL KMEQASQMTQ SVGCIPYMAP
2310 2320 2330 2340 2350
EVFKGDSNSE KSDVYSYGMV LFELLTSDEP QQDMKPMKMA HLAAYESYRP
2360 2370 2380 2390 2400
PIPLTTSSKW KEILTQCWDS NPDSRPTFKQ IIVHLKEMED QGVSSFASVP
2410
VQTIDTGVYA
Length:2,410
Mass (Da):264,919
Last modified:December 4, 2007 - v3
Checksum:i768EC59E9E05C229
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2074D → R in AAA33202 (PubMed:1972546).Curated1
Sequence conflicti2261V → L in AAA33202 (PubMed:1972546).Curated1
Sequence conflicti2282M → K in AAB41125 (PubMed:8898241).Curated1
Sequence conflicti2282M → K in AAA33202 (PubMed:1972546).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000055 Genomic DNA Translation: EAL65677.1
U32174 Genomic DNA Translation: AAB41125.1
M33785 mRNA Translation: AAA33202.1
PIRiT18276
RefSeqiXP_639040.1, XM_633948.1

Genome annotation databases

EnsemblProtistsiEAL65677; EAL65677; DDB_G0283385
GeneIDi8624065
KEGGiddi:DDB_G0283385

Entry informationi

Entry nameiSPLA_DICDI
AccessioniPrimary (citable) accession number: P18160
Secondary accession number(s): Q54R51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

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