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P18160

- SPLA_DICDI

UniProt

P18160 - SPLA_DICDI

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Protein
Dual specificity protein kinase splA
Gene
splA, DpyK1, pyK1, pykA, DDB_G0283385
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for spore differentiation.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei2142 – 21421ATP By similarity
Active sitei2243 – 22431Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi2121 – 21299ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein serine/threonine/tyrosine kinase activity Source: dictyBase
  4. protein tyrosine kinase activity Source: dictyBase

GO - Biological processi

  1. anatomical structure morphogenesis Source: dictyBase
  2. peptidyl-tyrosine phosphorylation Source: GOC
  3. sorocarp development Source: dictyBase
  4. sporulation resulting in formation of a cellular spore Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase splA (EC:2.7.10.2)
Alternative name(s):
Non-receptor tyrosine kinase spore lysis A
Tyrosine-protein kinase 1
Gene namesi
Name:splA
Synonyms:DpyK1, pyK1, pykA
ORF Names:DDB_G0283385
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283385. splA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24102410Dual specificity protein kinase splA
PRO_0000088121Add
BLAST

Post-translational modificationi

Tyrosine kinase domain is capable of autophosphorylation, in vitro; however it is also autophosphorylated on serine and threonine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP18160.

Expressioni

Developmental stagei

Expressed in vegetative cells and throughout development with a peak during the mound stage of morphogenesis.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0215670.

Structurei

3D structure databases

ProteinModelPortaliP18160.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini822 – 1004183B30.2/SPRY 1
Add
BLAST
Domaini1020 – 1209190B30.2/SPRY 2
Add
BLAST
Domaini1481 – 1703223B30.2/SPRY 3
Add
BLAST
Domaini1734 – 179865SAM
Add
BLAST
Domaini2115 – 2387273Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 5230Poly-Asn
Add
BLAST
Compositional biasi162 – 320159Ser-rich
Add
BLAST
Compositional biasi213 – 2197Poly-Asn
Compositional biasi235 – 24511Poly-Asn
Add
BLAST
Compositional biasi321 – 35232Poly-Gln
Add
BLAST
Compositional biasi447 – 51468Poly-Gln
Add
BLAST
Compositional biasi515 – 648134Pro-rich
Add
BLAST
Compositional biasi601 – 61010Poly-Asn
Compositional biasi631 – 759129Ser-rich
Add
BLAST
Compositional biasi706 – 72015Poly-Gln
Add
BLAST
Compositional biasi777 – 81337Poly-Asn
Add
BLAST
Compositional biasi1229 – 124618Poly-Asn
Add
BLAST
Compositional biasi1275 – 130632Poly-Asn
Add
BLAST
Compositional biasi1309 – 13179Poly-Asn
Compositional biasi1320 – 135839Poly-Asn
Add
BLAST
Compositional biasi1422 – 14265Poly-Asn
Compositional biasi1852 – 18554Poly-Ser
Compositional biasi2021 – 203616Poly-Asn
Add
BLAST
Compositional biasi2041 – 20466Poly-Gln
Compositional biasi2050 – 205910Poly-Gln
Compositional biasi2088 – 210316Pro-rich
Add
BLAST

Sequence similaritiesi

Contains 3 B30.2/SPRY domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
OMAiRWIVEDE.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR008985. ConA-like_lec_gl_sf.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF07647. SAM_2. 1 hit.
PF00622. SPRY. 3 hits.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
SM00449. SPRY. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49899. SSF49899. 3 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50188. B302_SPRY. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18160-1 [UniParc]FASTAAdd to Basket

« Hide

MNSKNDLFIG FFFFFYNYYY YYNNNNNNNN NNNNNNNNNN NNNNNNNNNN     50
NNIYIIVIIG LDPQQNHPSL KIPPPPSPTS PYVRRHARQH SRSNSSNSPG 100
ELTGGVEIIQ QQNSINTQTS PPTSTSPNTV PPPPPTNTTT SSTTITRNSN 150
NINNSNGGII NSSSGSNINN SSSSGVINTN INNSGGNISP TSNSLPSSNN 200
NILYTSSGSN SGNNNNNNNT INISSGSSGI NNSSNSNINN NNNNNSSSSS 250
GIHASGSLTA IPTNTNSNSS IFHSSNSVNR INKSNYSADS LVLPPNRISQ 300
VPQSQTQPQL QISPSTSFSS QQQQQQQLQQ QLQLQQQLQQ QIQQQQQIQQ 350
QQNLHFTYSK TFTSGQPNTL VNLSSPPPQS KHLHVSSDHS FFNEVLTPTP 400
VIHNSTNQNN QLLFGDSDPL SFLEYNNFIN RYQPALKNNQ PVSSYRQQQQ 450
IQHQIQLQQI QQQQQQQQQI QQQQLQQQQQ IQQQQIQQQQ QQIQQQQQQQ 500
QQQQQQQQQL QQIQPPPTIQ PPPQQTTPTL RGNRSSGNLS GLNSFSLKQS 550
TDSLTPPPNS QQSTVSSNST PIAATPISPL TAPTSPPPPP PPPTNFNSKF 600
NNNNNNNINN SSNNNTTVPP SPPPIIVLPK SPSSRSPRPA SAPVPSAPFL 650
VNNRVINTSS SSNISTNNTD LNLSSSSSSS PPLNISTASP SKSEDSPPTV 700
SPSYKQQQQQ QQQQQQQQQQ LNNSSNSSYS PKPRSPSVSS PPPSSISPNS 750
SPIGSPNISF HHHQQHQIPP PPPVLSNTNN NNNNNNNNNN NNNNNNNNNN 800
NNNNNNNNNN NTNHTNKKEG DSSWFNMSFK FFKKKLVPSN EYRWDLRKSN 850
SLTLNIEDKS RCSYRLPTSG SKGIAKSTQP FSSSFTYFEL FITNGNGDKI 900
CFGLTTNDHP IEVYPGNYQG SYGYSGDGKC YFGTNEGRVY GPSFSSGDVV 950
GCGYDSSSKT LYFTKNGVYL GVAAQKVNLI GLYPTVGLQN PGESVVINFF 1000
GPFSYRGAPE KPSKQSTIKD SGGSSIIPSE DLIPKEEFEV CRWSEKKNYH 1050
GKHVVVRNRT AFLPLDSPKD TIGGVRATQP FGEGFCYFEV IIDQLDKGQL 1100
SIGLANLEYP TFYHVGWMPR SYGYHNDDGR KFRWREEPGV NEGESYGSSY 1150
KKGDIIGCGL SFTSREIFFT KNGMYLGTAF SNVYGVFYPS VAFNEPGISI 1200
TGVFGPPFKF SQVTLMLKNV NSTSILVPNG NNNNNSNNNN NNNNNNIIGN 1250
GKITTTTTTS TSPSSINNNE DISSNNNNNN NNNNNNNNNN NNNNNNNNNN 1300
NNNNSNSSNT NNNNINNTTN NNNSNSNNNN NNNNSNSNSN SNNNNINNNN 1350
NNNNNNNNIY LTKKPSIGST DESSTGSLGG NNSSGNNNSS SGSIGNNSSI 1400
IKQRSPPHSI NGPLMLPPSS TNNNNNIYSS YNSTTAGSST TILPTLNHPI 1450
FGNTTSNNNS SSTLSVGGNN NLLGRHCQSL PITASTNHTL SSSLGVSFSS 1500
PSSSPKTSPR KIVNSSEDLG FVQTFQDQDG QPPSAWRRCG KSIKTKDDIT 1550
LTIIKKKTSV AMADRPFSSN SSSTICYFEV YLEGHDKKGS ITVGLSHSTY 1600
PFIKHIGREP KSYGFSSEGE KYGGSEIGEP YGPFFFFDGD SIASSCVIGC 1650
GINTSTRDIF FTKNGHYLGV AFSRVTSDPL YPSISFRGVV GGLCVATFPG 1700
GHFRFNIEDL PGISPSVWTE ALGPDRQGSG FKNWAPNDVA IWLESFNYGQ 1750
YRKNFRDNNI SGRHLEGITH AMLKNDLGIE PYGHREDIIN RLNRMIQIWN 1800
DKSPDSYPKI AIDSSDKIRW PASGGSSGGI NISGGVVIGS SSGSDDGITE 1850
ISSSSKNIRP YKSYTQKEIE DRNRRSTISG GEKKNKYYID NQMDPHQIGS 1900
MDSDGLLPDF GQGPPDEKNS SKTLSNEQIR YLQQRKDEPP IAISSTGNGG 1950
SVSSTGGSSG FLTFPSSNSL THPPQRDKPT QEFTHLPPIT SNYKGITNTG 2000
QPHKSFDQPL ELFPRHSAFS NNGNNGNNNN NNNNNNIKAN QQQQQQSSYQ 2050
QSQTQQQQQH ITSTSTSTTN KWIDPFGGWE TQSSLSHPPS RPPPPPPPPP 2100
QLPVRSEYEI DFNELEFGQT IGKGFFGEVK RGYWRETDVA IKIIYRDQFK 2150
TKSSLVMFQN EVGILSKLRH PNVVQFLGAC TAGGEDHHCI VTEWMGGGSL 2200
RQFLTDHFNL LEQNPHIRLK LALDIAKGMN YLHGWTPPIL HRDLSSRNIL 2250
LDHNIDPKNP VVSSRQDIKC KISDFGLSRL KMEQASQMTQ SVGCIPYMAP 2300
EVFKGDSNSE KSDVYSYGMV LFELLTSDEP QQDMKPMKMA HLAAYESYRP 2350
PIPLTTSSKW KEILTQCWDS NPDSRPTFKQ IIVHLKEMED QGVSSFASVP 2400
VQTIDTGVYA 2410
Length:2,410
Mass (Da):264,919
Last modified:December 4, 2007 - v3
Checksum:i768EC59E9E05C229
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2074 – 20741D → R in AAA33202. 1 Publication
Sequence conflicti2261 – 22611V → L in AAA33202. 1 Publication
Sequence conflicti2282 – 22821M → K in AAB41125. 1 Publication
Sequence conflicti2282 – 22821M → K in AAA33202. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000055 Genomic DNA. Translation: EAL65677.1.
U32174 Genomic DNA. Translation: AAB41125.1.
M33785 mRNA. Translation: AAA33202.1.
PIRiT18276.
RefSeqiXP_639040.1. XM_633948.1.

Genome annotation databases

EnsemblProtistsiDDB0252636; DDB0252636; DDB_G0283385.
GeneIDi8624065.
KEGGiddi:DDB_G0283385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000055 Genomic DNA. Translation: EAL65677.1 .
U32174 Genomic DNA. Translation: AAB41125.1 .
M33785 mRNA. Translation: AAA33202.1 .
PIRi T18276.
RefSeqi XP_639040.1. XM_633948.1.

3D structure databases

ProteinModelPortali P18160.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0215670.

Proteomic databases

PRIDEi P18160.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0252636 ; DDB0252636 ; DDB_G0283385 .
GeneIDi 8624065.
KEGGi ddi:DDB_G0283385.

Organism-specific databases

dictyBasei DDB_G0283385. splA.

Phylogenomic databases

eggNOGi COG0515.
OMAi RWIVEDE.

Enzyme and pathway databases

BRENDAi 2.7.12.1. 1939.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
InterProi IPR001870. B30.2/SPRY.
IPR008985. ConA-like_lec_gl_sf.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF07647. SAM_2. 1 hit.
PF00622. SPRY. 3 hits.
[Graphical view ]
SMARTi SM00454. SAM. 1 hit.
SM00449. SPRY. 3 hits.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF49899. SSF49899. 3 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50188. B302_SPRY. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The Dictyostelium dual-specificity kinase splA is essential for spore differentiation."
    Nuckolls G.H., Osherov N., Loomis W.F., Spudich J.A.
    Development 122:3295-3305(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 827-2410, AUTOPHOSPHORYLATION, DEVELOPMENTAL STAGE.
    Strain: JH10.
  3. "Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum."
    Tan J.L., Spudich J.A.
    Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2074-2410.

Entry informationi

Entry nameiSPLA_DICDI
AccessioniPrimary (citable) accession number: P18160
Secondary accession number(s): Q54R51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 4, 2007
Last modified: May 14, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3