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P18160

- SPLA_DICDI

UniProt

P18160 - SPLA_DICDI

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Protein

Dual specificity protein kinase splA

Gene

splA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential for spore differentiation.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei2142 – 21421ATPPROSITE-ProRule annotation
Active sitei2243 – 22431Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi2121 – 21299ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein serine/threonine/tyrosine kinase activity Source: dictyBase
  4. protein tyrosine kinase activity Source: dictyBase

GO - Biological processi

  1. anatomical structure morphogenesis Source: dictyBase
  2. peptidyl-tyrosine phosphorylation Source: GOC
  3. sorocarp development Source: dictyBase
  4. sporulation resulting in formation of a cellular spore Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase splA (EC:2.7.10.2)
Alternative name(s):
Non-receptor tyrosine kinase spore lysis A
Tyrosine-protein kinase 1
Gene namesi
Name:splA
Synonyms:DpyK1, pyK1, pykA
ORF Names:DDB_G0283385
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283385. splA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24102410Dual specificity protein kinase splAPRO_0000088121Add
BLAST

Post-translational modificationi

Tyrosine kinase domain is capable of autophosphorylation, in vitro; however it is also autophosphorylated on serine and threonine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP18160.

Expressioni

Developmental stagei

Expressed in vegetative cells and throughout development with a peak during the mound stage of morphogenesis.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0215670.

Structurei

3D structure databases

ProteinModelPortaliP18160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini822 – 1004183B30.2/SPRY 1PROSITE-ProRule annotationAdd
BLAST
Domaini1020 – 1209190B30.2/SPRY 2PROSITE-ProRule annotationAdd
BLAST
Domaini1481 – 1703223B30.2/SPRY 3PROSITE-ProRule annotationAdd
BLAST
Domaini1734 – 179865SAMPROSITE-ProRule annotationAdd
BLAST
Domaini2115 – 2387273Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 5230Poly-AsnAdd
BLAST
Compositional biasi162 – 320159Ser-richAdd
BLAST
Compositional biasi213 – 2197Poly-Asn
Compositional biasi235 – 24511Poly-AsnAdd
BLAST
Compositional biasi321 – 35232Poly-GlnAdd
BLAST
Compositional biasi447 – 51468Poly-GlnAdd
BLAST
Compositional biasi515 – 648134Pro-richAdd
BLAST
Compositional biasi601 – 61010Poly-Asn
Compositional biasi631 – 759129Ser-richAdd
BLAST
Compositional biasi706 – 72015Poly-GlnAdd
BLAST
Compositional biasi777 – 81337Poly-AsnAdd
BLAST
Compositional biasi1229 – 124618Poly-AsnAdd
BLAST
Compositional biasi1275 – 130632Poly-AsnAdd
BLAST
Compositional biasi1309 – 13179Poly-Asn
Compositional biasi1320 – 135839Poly-AsnAdd
BLAST
Compositional biasi1422 – 14265Poly-Asn
Compositional biasi1852 – 18554Poly-Ser
Compositional biasi2021 – 203616Poly-AsnAdd
BLAST
Compositional biasi2041 – 20466Poly-Gln
Compositional biasi2050 – 205910Poly-Gln
Compositional biasi2088 – 210316Pro-richAdd
BLAST

Sequence similaritiesi

Contains 3 B30.2/SPRY domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP18160.
OMAiRWIVEDE.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR003877. SPRY_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF07647. SAM_2. 1 hit.
PF00622. SPRY. 3 hits.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
SM00449. SPRY. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49899. SSF49899. 3 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50188. B302_SPRY. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18160-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSKNDLFIG FFFFFYNYYY YYNNNNNNNN NNNNNNNNNN NNNNNNNNNN
60 70 80 90 100
NNIYIIVIIG LDPQQNHPSL KIPPPPSPTS PYVRRHARQH SRSNSSNSPG
110 120 130 140 150
ELTGGVEIIQ QQNSINTQTS PPTSTSPNTV PPPPPTNTTT SSTTITRNSN
160 170 180 190 200
NINNSNGGII NSSSGSNINN SSSSGVINTN INNSGGNISP TSNSLPSSNN
210 220 230 240 250
NILYTSSGSN SGNNNNNNNT INISSGSSGI NNSSNSNINN NNNNNSSSSS
260 270 280 290 300
GIHASGSLTA IPTNTNSNSS IFHSSNSVNR INKSNYSADS LVLPPNRISQ
310 320 330 340 350
VPQSQTQPQL QISPSTSFSS QQQQQQQLQQ QLQLQQQLQQ QIQQQQQIQQ
360 370 380 390 400
QQNLHFTYSK TFTSGQPNTL VNLSSPPPQS KHLHVSSDHS FFNEVLTPTP
410 420 430 440 450
VIHNSTNQNN QLLFGDSDPL SFLEYNNFIN RYQPALKNNQ PVSSYRQQQQ
460 470 480 490 500
IQHQIQLQQI QQQQQQQQQI QQQQLQQQQQ IQQQQIQQQQ QQIQQQQQQQ
510 520 530 540 550
QQQQQQQQQL QQIQPPPTIQ PPPQQTTPTL RGNRSSGNLS GLNSFSLKQS
560 570 580 590 600
TDSLTPPPNS QQSTVSSNST PIAATPISPL TAPTSPPPPP PPPTNFNSKF
610 620 630 640 650
NNNNNNNINN SSNNNTTVPP SPPPIIVLPK SPSSRSPRPA SAPVPSAPFL
660 670 680 690 700
VNNRVINTSS SSNISTNNTD LNLSSSSSSS PPLNISTASP SKSEDSPPTV
710 720 730 740 750
SPSYKQQQQQ QQQQQQQQQQ LNNSSNSSYS PKPRSPSVSS PPPSSISPNS
760 770 780 790 800
SPIGSPNISF HHHQQHQIPP PPPVLSNTNN NNNNNNNNNN NNNNNNNNNN
810 820 830 840 850
NNNNNNNNNN NTNHTNKKEG DSSWFNMSFK FFKKKLVPSN EYRWDLRKSN
860 870 880 890 900
SLTLNIEDKS RCSYRLPTSG SKGIAKSTQP FSSSFTYFEL FITNGNGDKI
910 920 930 940 950
CFGLTTNDHP IEVYPGNYQG SYGYSGDGKC YFGTNEGRVY GPSFSSGDVV
960 970 980 990 1000
GCGYDSSSKT LYFTKNGVYL GVAAQKVNLI GLYPTVGLQN PGESVVINFF
1010 1020 1030 1040 1050
GPFSYRGAPE KPSKQSTIKD SGGSSIIPSE DLIPKEEFEV CRWSEKKNYH
1060 1070 1080 1090 1100
GKHVVVRNRT AFLPLDSPKD TIGGVRATQP FGEGFCYFEV IIDQLDKGQL
1110 1120 1130 1140 1150
SIGLANLEYP TFYHVGWMPR SYGYHNDDGR KFRWREEPGV NEGESYGSSY
1160 1170 1180 1190 1200
KKGDIIGCGL SFTSREIFFT KNGMYLGTAF SNVYGVFYPS VAFNEPGISI
1210 1220 1230 1240 1250
TGVFGPPFKF SQVTLMLKNV NSTSILVPNG NNNNNSNNNN NNNNNNIIGN
1260 1270 1280 1290 1300
GKITTTTTTS TSPSSINNNE DISSNNNNNN NNNNNNNNNN NNNNNNNNNN
1310 1320 1330 1340 1350
NNNNSNSSNT NNNNINNTTN NNNSNSNNNN NNNNSNSNSN SNNNNINNNN
1360 1370 1380 1390 1400
NNNNNNNNIY LTKKPSIGST DESSTGSLGG NNSSGNNNSS SGSIGNNSSI
1410 1420 1430 1440 1450
IKQRSPPHSI NGPLMLPPSS TNNNNNIYSS YNSTTAGSST TILPTLNHPI
1460 1470 1480 1490 1500
FGNTTSNNNS SSTLSVGGNN NLLGRHCQSL PITASTNHTL SSSLGVSFSS
1510 1520 1530 1540 1550
PSSSPKTSPR KIVNSSEDLG FVQTFQDQDG QPPSAWRRCG KSIKTKDDIT
1560 1570 1580 1590 1600
LTIIKKKTSV AMADRPFSSN SSSTICYFEV YLEGHDKKGS ITVGLSHSTY
1610 1620 1630 1640 1650
PFIKHIGREP KSYGFSSEGE KYGGSEIGEP YGPFFFFDGD SIASSCVIGC
1660 1670 1680 1690 1700
GINTSTRDIF FTKNGHYLGV AFSRVTSDPL YPSISFRGVV GGLCVATFPG
1710 1720 1730 1740 1750
GHFRFNIEDL PGISPSVWTE ALGPDRQGSG FKNWAPNDVA IWLESFNYGQ
1760 1770 1780 1790 1800
YRKNFRDNNI SGRHLEGITH AMLKNDLGIE PYGHREDIIN RLNRMIQIWN
1810 1820 1830 1840 1850
DKSPDSYPKI AIDSSDKIRW PASGGSSGGI NISGGVVIGS SSGSDDGITE
1860 1870 1880 1890 1900
ISSSSKNIRP YKSYTQKEIE DRNRRSTISG GEKKNKYYID NQMDPHQIGS
1910 1920 1930 1940 1950
MDSDGLLPDF GQGPPDEKNS SKTLSNEQIR YLQQRKDEPP IAISSTGNGG
1960 1970 1980 1990 2000
SVSSTGGSSG FLTFPSSNSL THPPQRDKPT QEFTHLPPIT SNYKGITNTG
2010 2020 2030 2040 2050
QPHKSFDQPL ELFPRHSAFS NNGNNGNNNN NNNNNNIKAN QQQQQQSSYQ
2060 2070 2080 2090 2100
QSQTQQQQQH ITSTSTSTTN KWIDPFGGWE TQSSLSHPPS RPPPPPPPPP
2110 2120 2130 2140 2150
QLPVRSEYEI DFNELEFGQT IGKGFFGEVK RGYWRETDVA IKIIYRDQFK
2160 2170 2180 2190 2200
TKSSLVMFQN EVGILSKLRH PNVVQFLGAC TAGGEDHHCI VTEWMGGGSL
2210 2220 2230 2240 2250
RQFLTDHFNL LEQNPHIRLK LALDIAKGMN YLHGWTPPIL HRDLSSRNIL
2260 2270 2280 2290 2300
LDHNIDPKNP VVSSRQDIKC KISDFGLSRL KMEQASQMTQ SVGCIPYMAP
2310 2320 2330 2340 2350
EVFKGDSNSE KSDVYSYGMV LFELLTSDEP QQDMKPMKMA HLAAYESYRP
2360 2370 2380 2390 2400
PIPLTTSSKW KEILTQCWDS NPDSRPTFKQ IIVHLKEMED QGVSSFASVP
2410
VQTIDTGVYA
Length:2,410
Mass (Da):264,919
Last modified:December 4, 2007 - v3
Checksum:i768EC59E9E05C229
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2074 – 20741D → R in AAA33202. (PubMed:1972546)Curated
Sequence conflicti2261 – 22611V → L in AAA33202. (PubMed:1972546)Curated
Sequence conflicti2282 – 22821M → K in AAB41125. (PubMed:8898241)Curated
Sequence conflicti2282 – 22821M → K in AAA33202. (PubMed:1972546)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000055 Genomic DNA. Translation: EAL65677.1.
U32174 Genomic DNA. Translation: AAB41125.1.
M33785 mRNA. Translation: AAA33202.1.
PIRiT18276.
RefSeqiXP_639040.1. XM_633948.1.

Genome annotation databases

EnsemblProtistsiDDB0252636; DDB0252636; DDB_G0283385.
GeneIDi8624065.
KEGGiddi:DDB_G0283385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000055 Genomic DNA. Translation: EAL65677.1 .
U32174 Genomic DNA. Translation: AAB41125.1 .
M33785 mRNA. Translation: AAA33202.1 .
PIRi T18276.
RefSeqi XP_639040.1. XM_633948.1.

3D structure databases

ProteinModelPortali P18160.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0215670.

Proteomic databases

PRIDEi P18160.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0252636 ; DDB0252636 ; DDB_G0283385 .
GeneIDi 8624065.
KEGGi ddi:DDB_G0283385.

Organism-specific databases

dictyBasei DDB_G0283385. splA.

Phylogenomic databases

eggNOGi COG0515.
InParanoidi P18160.
OMAi RWIVEDE.

Enzyme and pathway databases

BRENDAi 2.7.12.1. 1939.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
InterProi IPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR003877. SPRY_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF07647. SAM_2. 1 hit.
PF00622. SPRY. 3 hits.
[Graphical view ]
SMARTi SM00454. SAM. 1 hit.
SM00449. SPRY. 3 hits.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF49899. SSF49899. 3 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50188. B302_SPRY. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The Dictyostelium dual-specificity kinase splA is essential for spore differentiation."
    Nuckolls G.H., Osherov N., Loomis W.F., Spudich J.A.
    Development 122:3295-3305(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 827-2410, AUTOPHOSPHORYLATION, DEVELOPMENTAL STAGE.
    Strain: JH10.
  3. "Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum."
    Tan J.L., Spudich J.A.
    Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2074-2410.

Entry informationi

Entry nameiSPLA_DICDI
AccessioniPrimary (citable) accession number: P18160
Secondary accession number(s): Q54R51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3