Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P18160 (SPLA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity protein kinase splA

EC=2.7.10.2
Alternative name(s):
Non-receptor tyrosine kinase spore lysis A
Tyrosine-protein kinase 1
Gene names
Name:splA
Synonyms:DpyK1, pyK1, pykA
ORF Names:DDB_G0283385
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length2410 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for spore differentiation.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Developmental stage

Expressed in vegetative cells and throughout development with a peak during the mound stage of morphogenesis. Ref.2

Post-translational modification

Tyrosine kinase domain is capable of autophosphorylation, in vitro; however it is also autophosphorylated on serine and threonine residues. Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. TKL Tyr protein kinase family.

Contains 3 B30.2/SPRY domains.

Contains 1 protein kinase domain.

Contains 1 SAM (sterile alpha motif) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 24102410Dual specificity protein kinase splA
PRO_0000088121

Regions

Domain822 – 1004183B30.2/SPRY 1
Domain1020 – 1209190B30.2/SPRY 2
Domain1481 – 1703223B30.2/SPRY 3
Domain1734 – 179865SAM
Domain2115 – 2387273Protein kinase
Nucleotide binding2121 – 21299ATP By similarity
Compositional bias23 – 5230Poly-Asn
Compositional bias162 – 320159Ser-rich
Compositional bias213 – 2197Poly-Asn
Compositional bias235 – 24511Poly-Asn
Compositional bias321 – 35232Poly-Gln
Compositional bias447 – 51468Poly-Gln
Compositional bias515 – 648134Pro-rich
Compositional bias601 – 61010Poly-Asn
Compositional bias631 – 759129Ser-rich
Compositional bias706 – 72015Poly-Gln
Compositional bias777 – 81337Poly-Asn
Compositional bias1229 – 124618Poly-Asn
Compositional bias1275 – 130632Poly-Asn
Compositional bias1309 – 13179Poly-Asn
Compositional bias1320 – 135839Poly-Asn
Compositional bias1422 – 14265Poly-Asn
Compositional bias1852 – 18554Poly-Ser
Compositional bias2021 – 203616Poly-Asn
Compositional bias2041 – 20466Poly-Gln
Compositional bias2050 – 205910Poly-Gln
Compositional bias2088 – 210316Pro-rich

Sites

Active site22431Proton acceptor By similarity
Binding site21421ATP By similarity

Experimental info

Sequence conflict20741D → R in AAA33202. Ref.3
Sequence conflict22611V → L in AAA33202. Ref.3
Sequence conflict22821M → K in AAB41125. Ref.2
Sequence conflict22821M → K in AAA33202. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P18160 [UniParc].

Last modified December 4, 2007. Version 3.
Checksum: 768EC59E9E05C229

FASTA2,410264,919
        10         20         30         40         50         60 
MNSKNDLFIG FFFFFYNYYY YYNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNIYIIVIIG 

        70         80         90        100        110        120 
LDPQQNHPSL KIPPPPSPTS PYVRRHARQH SRSNSSNSPG ELTGGVEIIQ QQNSINTQTS 

       130        140        150        160        170        180 
PPTSTSPNTV PPPPPTNTTT SSTTITRNSN NINNSNGGII NSSSGSNINN SSSSGVINTN 

       190        200        210        220        230        240 
INNSGGNISP TSNSLPSSNN NILYTSSGSN SGNNNNNNNT INISSGSSGI NNSSNSNINN 

       250        260        270        280        290        300 
NNNNNSSSSS GIHASGSLTA IPTNTNSNSS IFHSSNSVNR INKSNYSADS LVLPPNRISQ 

       310        320        330        340        350        360 
VPQSQTQPQL QISPSTSFSS QQQQQQQLQQ QLQLQQQLQQ QIQQQQQIQQ QQNLHFTYSK 

       370        380        390        400        410        420 
TFTSGQPNTL VNLSSPPPQS KHLHVSSDHS FFNEVLTPTP VIHNSTNQNN QLLFGDSDPL 

       430        440        450        460        470        480 
SFLEYNNFIN RYQPALKNNQ PVSSYRQQQQ IQHQIQLQQI QQQQQQQQQI QQQQLQQQQQ 

       490        500        510        520        530        540 
IQQQQIQQQQ QQIQQQQQQQ QQQQQQQQQL QQIQPPPTIQ PPPQQTTPTL RGNRSSGNLS 

       550        560        570        580        590        600 
GLNSFSLKQS TDSLTPPPNS QQSTVSSNST PIAATPISPL TAPTSPPPPP PPPTNFNSKF 

       610        620        630        640        650        660 
NNNNNNNINN SSNNNTTVPP SPPPIIVLPK SPSSRSPRPA SAPVPSAPFL VNNRVINTSS 

       670        680        690        700        710        720 
SSNISTNNTD LNLSSSSSSS PPLNISTASP SKSEDSPPTV SPSYKQQQQQ QQQQQQQQQQ 

       730        740        750        760        770        780 
LNNSSNSSYS PKPRSPSVSS PPPSSISPNS SPIGSPNISF HHHQQHQIPP PPPVLSNTNN 

       790        800        810        820        830        840 
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTNHTNKKEG DSSWFNMSFK FFKKKLVPSN 

       850        860        870        880        890        900 
EYRWDLRKSN SLTLNIEDKS RCSYRLPTSG SKGIAKSTQP FSSSFTYFEL FITNGNGDKI 

       910        920        930        940        950        960 
CFGLTTNDHP IEVYPGNYQG SYGYSGDGKC YFGTNEGRVY GPSFSSGDVV GCGYDSSSKT 

       970        980        990       1000       1010       1020 
LYFTKNGVYL GVAAQKVNLI GLYPTVGLQN PGESVVINFF GPFSYRGAPE KPSKQSTIKD 

      1030       1040       1050       1060       1070       1080 
SGGSSIIPSE DLIPKEEFEV CRWSEKKNYH GKHVVVRNRT AFLPLDSPKD TIGGVRATQP 

      1090       1100       1110       1120       1130       1140 
FGEGFCYFEV IIDQLDKGQL SIGLANLEYP TFYHVGWMPR SYGYHNDDGR KFRWREEPGV 

      1150       1160       1170       1180       1190       1200 
NEGESYGSSY KKGDIIGCGL SFTSREIFFT KNGMYLGTAF SNVYGVFYPS VAFNEPGISI 

      1210       1220       1230       1240       1250       1260 
TGVFGPPFKF SQVTLMLKNV NSTSILVPNG NNNNNSNNNN NNNNNNIIGN GKITTTTTTS 

      1270       1280       1290       1300       1310       1320 
TSPSSINNNE DISSNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNSNSSNT NNNNINNTTN 

      1330       1340       1350       1360       1370       1380 
NNNSNSNNNN NNNNSNSNSN SNNNNINNNN NNNNNNNNIY LTKKPSIGST DESSTGSLGG 

      1390       1400       1410       1420       1430       1440 
NNSSGNNNSS SGSIGNNSSI IKQRSPPHSI NGPLMLPPSS TNNNNNIYSS YNSTTAGSST 

      1450       1460       1470       1480       1490       1500 
TILPTLNHPI FGNTTSNNNS SSTLSVGGNN NLLGRHCQSL PITASTNHTL SSSLGVSFSS 

      1510       1520       1530       1540       1550       1560 
PSSSPKTSPR KIVNSSEDLG FVQTFQDQDG QPPSAWRRCG KSIKTKDDIT LTIIKKKTSV 

      1570       1580       1590       1600       1610       1620 
AMADRPFSSN SSSTICYFEV YLEGHDKKGS ITVGLSHSTY PFIKHIGREP KSYGFSSEGE 

      1630       1640       1650       1660       1670       1680 
KYGGSEIGEP YGPFFFFDGD SIASSCVIGC GINTSTRDIF FTKNGHYLGV AFSRVTSDPL 

      1690       1700       1710       1720       1730       1740 
YPSISFRGVV GGLCVATFPG GHFRFNIEDL PGISPSVWTE ALGPDRQGSG FKNWAPNDVA 

      1750       1760       1770       1780       1790       1800 
IWLESFNYGQ YRKNFRDNNI SGRHLEGITH AMLKNDLGIE PYGHREDIIN RLNRMIQIWN 

      1810       1820       1830       1840       1850       1860 
DKSPDSYPKI AIDSSDKIRW PASGGSSGGI NISGGVVIGS SSGSDDGITE ISSSSKNIRP 

      1870       1880       1890       1900       1910       1920 
YKSYTQKEIE DRNRRSTISG GEKKNKYYID NQMDPHQIGS MDSDGLLPDF GQGPPDEKNS 

      1930       1940       1950       1960       1970       1980 
SKTLSNEQIR YLQQRKDEPP IAISSTGNGG SVSSTGGSSG FLTFPSSNSL THPPQRDKPT 

      1990       2000       2010       2020       2030       2040 
QEFTHLPPIT SNYKGITNTG QPHKSFDQPL ELFPRHSAFS NNGNNGNNNN NNNNNNIKAN 

      2050       2060       2070       2080       2090       2100 
QQQQQQSSYQ QSQTQQQQQH ITSTSTSTTN KWIDPFGGWE TQSSLSHPPS RPPPPPPPPP 

      2110       2120       2130       2140       2150       2160 
QLPVRSEYEI DFNELEFGQT IGKGFFGEVK RGYWRETDVA IKIIYRDQFK TKSSLVMFQN 

      2170       2180       2190       2200       2210       2220 
EVGILSKLRH PNVVQFLGAC TAGGEDHHCI VTEWMGGGSL RQFLTDHFNL LEQNPHIRLK 

      2230       2240       2250       2260       2270       2280 
LALDIAKGMN YLHGWTPPIL HRDLSSRNIL LDHNIDPKNP VVSSRQDIKC KISDFGLSRL 

      2290       2300       2310       2320       2330       2340 
KMEQASQMTQ SVGCIPYMAP EVFKGDSNSE KSDVYSYGMV LFELLTSDEP QQDMKPMKMA 

      2350       2360       2370       2380       2390       2400 
HLAAYESYRP PIPLTTSSKW KEILTQCWDS NPDSRPTFKQ IIVHLKEMED QGVSSFASVP 

      2410 
VQTIDTGVYA 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The Dictyostelium dual-specificity kinase splA is essential for spore differentiation."
Nuckolls G.H., Osherov N., Loomis W.F., Spudich J.A.
Development 122:3295-3305(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 827-2410, AUTOPHOSPHORYLATION, DEVELOPMENTAL STAGE.
Strain: JH10.
[3]"Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum."
Tan J.L., Spudich J.A.
Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2074-2410.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000055 Genomic DNA. Translation: EAL65677.1.
U32174 Genomic DNA. Translation: AAB41125.1.
M33785 mRNA. Translation: AAA33202.1.
PIRT18276.
RefSeqXP_639040.1. XM_633948.1.

3D structure databases

ProteinModelPortalP18160.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0215670.

Proteomic databases

PRIDEP18160.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0252636; DDB0252636; DDB_G0283385.
GeneID8624065.
KEGGddi:DDB_G0283385.

Organism-specific databases

dictyBaseDDB_G0283385. splA.

Phylogenomic databases

eggNOGCOG0515.
OMARWIVEDE.

Enzyme and pathway databases

BRENDA2.7.12.1. 1939.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
InterProIPR001870. B30.2/SPRY.
IPR008985. ConA-like_lec_gl_sf.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
PF07647. SAM_2. 1 hit.
PF00622. SPRY. 3 hits.
[Graphical view]
SMARTSM00454. SAM. 1 hit.
SM00449. SPRY. 3 hits.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
SSF49899. SSF49899. 3 hits.
SSF56112. SSF56112. 1 hit.
PROSITEPS50188. B302_SPRY. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPLA_DICDI
AccessionPrimary (citable) accession number: P18160
Secondary accession number(s): Q54R51
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 4, 2007
Last modified: May 14, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase