P18160 (SPLA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein kinase splA
EC=2.7.10.2

Alternative name(s):
Non-receptor tyrosine kinase spore lysis A
Tyrosine-protein kinase 1

Gene names

Name:	splA
Synonyms:	DpyK1, pyK1, pykA
ORF Names:	DDB_G0283385

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	2410 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for spore differentiation.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Developmental stage	Expressed in vegetative cells and throughout development with a peak during the mound stage of morphogenesis. Ref.2
Post-translational modification	Tyrosine kinase domain is capable of autophosphorylation, in vitro; however it is also autophosphorylated on serine and threonine residues. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. TKL Tyr protein kinase family. Contains 3 B30.2/SPRY domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sorocarp development Inferred from mutant phenotype PubMed 17659086. Source: dictyBase sporulation resulting in formation of a cellular spore Inferred from mutant phenotype Ref.2. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine/tyrosine kinase activity Inferred from direct assay Ref.2. Source: dictyBase protein tyrosine kinase activity Inferred from direct assay Ref.3. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2410	2410	Dual specificity protein kinase splA		PRO_0000088121
Regions
	Domain	822 – 1004	183	B30.2/SPRY 1
	Domain	1020 – 1209	190	B30.2/SPRY 2
	Domain	1481 – 1703	223	B30.2/SPRY 3
	Domain	1734 – 1798	65	SAM
	Domain	2115 – 2387	273	Protein kinase
	Nucleotide binding	2121 – 2129	9	ATP By similarity
	Compositional bias	23 – 52	30	Poly-Asn
	Compositional bias	162 – 320	159	Ser-rich
	Compositional bias	213 – 219	7	Poly-Asn
	Compositional bias	235 – 245	11	Poly-Asn
	Compositional bias	321 – 352	32	Poly-Gln
	Compositional bias	447 – 514	68	Poly-Gln
	Compositional bias	515 – 648	134	Pro-rich
	Compositional bias	601 – 610	10	Poly-Asn
	Compositional bias	631 – 759	129	Ser-rich
	Compositional bias	706 – 720	15	Poly-Gln
	Compositional bias	777 – 813	37	Poly-Asn
	Compositional bias	1229 – 1246	18	Poly-Asn
	Compositional bias	1275 – 1306	32	Poly-Asn
	Compositional bias	1309 – 1317	9	Poly-Asn
	Compositional bias	1320 – 1358	39	Poly-Asn
	Compositional bias	1422 – 1426	5	Poly-Asn
	Compositional bias	1852 – 1855	4	Poly-Ser
	Compositional bias	2021 – 2036	16	Poly-Asn
	Compositional bias	2041 – 2046	6	Poly-Gln
	Compositional bias	2050 – 2059	10	Poly-Gln
	Compositional bias	2088 – 2103	16	Pro-rich
Sites
	Active site	2243	1	Proton acceptor By similarity
	Binding site	2142	1	ATP By similarity
Experimental info
	Sequence conflict	2074	1	D → R in AAA33202. Ref.3
	Sequence conflict	2261	1	V → L in AAA33202. Ref.3
	Sequence conflict	2282	1	M → K in AAB41125. Ref.2
	Sequence conflict	2282	1	M → K in AAA33202. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P18160 [UniParc]. Last modified December 4, 2007. Version 3. Checksum: 768EC59E9E05C229	FASTA	2,410	264,919
	10 20 30 40 50 60 MNSKNDLFIG FFFFFYNYYY YYNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNIYIIVIIG 70 80 90 100 110 120 LDPQQNHPSL KIPPPPSPTS PYVRRHARQH SRSNSSNSPG ELTGGVEIIQ QQNSINTQTS 130 140 150 160 170 180 PPTSTSPNTV PPPPPTNTTT SSTTITRNSN NINNSNGGII NSSSGSNINN SSSSGVINTN 190 200 210 220 230 240 INNSGGNISP TSNSLPSSNN NILYTSSGSN SGNNNNNNNT INISSGSSGI NNSSNSNINN 250 260 270 280 290 300 NNNNNSSSSS GIHASGSLTA IPTNTNSNSS IFHSSNSVNR INKSNYSADS LVLPPNRISQ 310 320 330 340 350 360 VPQSQTQPQL QISPSTSFSS QQQQQQQLQQ QLQLQQQLQQ QIQQQQQIQQ QQNLHFTYSK 370 380 390 400 410 420 TFTSGQPNTL VNLSSPPPQS KHLHVSSDHS FFNEVLTPTP VIHNSTNQNN QLLFGDSDPL 430 440 450 460 470 480 SFLEYNNFIN RYQPALKNNQ PVSSYRQQQQ IQHQIQLQQI QQQQQQQQQI QQQQLQQQQQ 490 500 510 520 530 540 IQQQQIQQQQ QQIQQQQQQQ QQQQQQQQQL QQIQPPPTIQ PPPQQTTPTL RGNRSSGNLS 550 560 570 580 590 600 GLNSFSLKQS TDSLTPPPNS QQSTVSSNST PIAATPISPL TAPTSPPPPP PPPTNFNSKF 610 620 630 640 650 660 NNNNNNNINN SSNNNTTVPP SPPPIIVLPK SPSSRSPRPA SAPVPSAPFL VNNRVINTSS 670 680 690 700 710 720 SSNISTNNTD LNLSSSSSSS PPLNISTASP SKSEDSPPTV SPSYKQQQQQ QQQQQQQQQQ 730 740 750 760 770 780 LNNSSNSSYS PKPRSPSVSS PPPSSISPNS SPIGSPNISF HHHQQHQIPP PPPVLSNTNN 790 800 810 820 830 840 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTNHTNKKEG DSSWFNMSFK FFKKKLVPSN 850 860 870 880 890 900 EYRWDLRKSN SLTLNIEDKS RCSYRLPTSG SKGIAKSTQP FSSSFTYFEL FITNGNGDKI 910 920 930 940 950 960 CFGLTTNDHP IEVYPGNYQG SYGYSGDGKC YFGTNEGRVY GPSFSSGDVV GCGYDSSSKT 970 980 990 1000 1010 1020 LYFTKNGVYL GVAAQKVNLI GLYPTVGLQN PGESVVINFF GPFSYRGAPE KPSKQSTIKD 1030 1040 1050 1060 1070 1080 SGGSSIIPSE DLIPKEEFEV CRWSEKKNYH GKHVVVRNRT AFLPLDSPKD TIGGVRATQP 1090 1100 1110 1120 1130 1140 FGEGFCYFEV IIDQLDKGQL SIGLANLEYP TFYHVGWMPR SYGYHNDDGR KFRWREEPGV 1150 1160 1170 1180 1190 1200 NEGESYGSSY KKGDIIGCGL SFTSREIFFT KNGMYLGTAF SNVYGVFYPS VAFNEPGISI 1210 1220 1230 1240 1250 1260 TGVFGPPFKF SQVTLMLKNV NSTSILVPNG NNNNNSNNNN NNNNNNIIGN GKITTTTTTS 1270 1280 1290 1300 1310 1320 TSPSSINNNE DISSNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNSNSSNT NNNNINNTTN 1330 1340 1350 1360 1370 1380 NNNSNSNNNN NNNNSNSNSN SNNNNINNNN NNNNNNNNIY LTKKPSIGST DESSTGSLGG 1390 1400 1410 1420 1430 1440 NNSSGNNNSS SGSIGNNSSI IKQRSPPHSI NGPLMLPPSS TNNNNNIYSS YNSTTAGSST 1450 1460 1470 1480 1490 1500 TILPTLNHPI FGNTTSNNNS SSTLSVGGNN NLLGRHCQSL PITASTNHTL SSSLGVSFSS 1510 1520 1530 1540 1550 1560 PSSSPKTSPR KIVNSSEDLG FVQTFQDQDG QPPSAWRRCG KSIKTKDDIT LTIIKKKTSV 1570 1580 1590 1600 1610 1620 AMADRPFSSN SSSTICYFEV YLEGHDKKGS ITVGLSHSTY PFIKHIGREP KSYGFSSEGE 1630 1640 1650 1660 1670 1680 KYGGSEIGEP YGPFFFFDGD SIASSCVIGC GINTSTRDIF FTKNGHYLGV AFSRVTSDPL 1690 1700 1710 1720 1730 1740 YPSISFRGVV GGLCVATFPG GHFRFNIEDL PGISPSVWTE ALGPDRQGSG FKNWAPNDVA 1750 1760 1770 1780 1790 1800 IWLESFNYGQ YRKNFRDNNI SGRHLEGITH AMLKNDLGIE PYGHREDIIN RLNRMIQIWN 1810 1820 1830 1840 1850 1860 DKSPDSYPKI AIDSSDKIRW PASGGSSGGI NISGGVVIGS SSGSDDGITE ISSSSKNIRP 1870 1880 1890 1900 1910 1920 YKSYTQKEIE DRNRRSTISG GEKKNKYYID NQMDPHQIGS MDSDGLLPDF GQGPPDEKNS 1930 1940 1950 1960 1970 1980 SKTLSNEQIR YLQQRKDEPP IAISSTGNGG SVSSTGGSSG FLTFPSSNSL THPPQRDKPT 1990 2000 2010 2020 2030 2040 QEFTHLPPIT SNYKGITNTG QPHKSFDQPL ELFPRHSAFS NNGNNGNNNN NNNNNNIKAN 2050 2060 2070 2080 2090 2100 QQQQQQSSYQ QSQTQQQQQH ITSTSTSTTN KWIDPFGGWE TQSSLSHPPS RPPPPPPPPP 2110 2120 2130 2140 2150 2160 QLPVRSEYEI DFNELEFGQT IGKGFFGEVK RGYWRETDVA IKIIYRDQFK TKSSLVMFQN 2170 2180 2190 2200 2210 2220 EVGILSKLRH PNVVQFLGAC TAGGEDHHCI VTEWMGGGSL RQFLTDHFNL LEQNPHIRLK 2230 2240 2250 2260 2270 2280 LALDIAKGMN YLHGWTPPIL HRDLSSRNIL LDHNIDPKNP VVSSRQDIKC KISDFGLSRL 2290 2300 2310 2320 2330 2340 KMEQASQMTQ SVGCIPYMAP EVFKGDSNSE KSDVYSYGMV LFELLTSDEP QQDMKPMKMA 2350 2360 2370 2380 2390 2400 HLAAYESYRP PIPLTTSSKW KEILTQCWDS NPDSRPTFKQ IIVHLKEMED QGVSSFASVP 2410 VQTIDTGVYA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The Dictyostelium dual-specificity kinase splA is essential for spore differentiation." Nuckolls G.H., Osherov N., Loomis W.F., Spudich J.A. Development 122:3295-3305(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 827-2410, AUTOPHOSPHORYLATION, DEVELOPMENTAL STAGE. Strain: JH10.
[3]	"Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum." Tan J.L., Spudich J.A. Mol. Cell. Biol. 10:3578-3583(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2074-2410.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000055 Genomic DNA. Translation: EAL65677.1. U32174 Genomic DNA. Translation: AAB41125.1. M33785 mRNA. Translation: AAA33202.1.
PIR	T18276.
RefSeq	XP_639040.1. XM_633948.1.
3D structure databases
ProteinModelPortal	P18160.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0252636; DDB0252636; DDB_G0283385.
GeneID	8624065.
KEGG	ddi:DDB_G0283385.
Organism-specific databases
dictyBase	DDB_G0283385. splA.
Phylogenomic databases
eggNOG	COG0515.
Enzyme and pathway databases
BRENDA	2.7.12.1. 1939.
Family and domain databases
Gene3D	1.10.150.50. 1 hit.
InterPro	IPR001870. B30.2/SPRY. IPR008985. ConA-like_lec_gl_sf. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR013761. SAM/pointed. IPR011510. SAM_2. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR018355. SPla/RYanodine_receptor_subgr. IPR003877. SPRY_rcpt. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF07647. SAM_2. 1 hit. PF00622. SPRY. 3 hits. [Graphical view]
SMART	SM00454. SAM. 1 hit. SM00449. SPRY. 3 hits. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 3 hits. SSF56112. Kinase_like. 1 hit. SSF47769. SAM_homology. 1 hit.
PROSITE	PS50188. B302_SPRY. 3 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPLA_DICDI

Accession

Primary (citable) accession number: P18160
Secondary accession number(s): Q54R51

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)