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P18158 (GLPD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aerobic glycerol-3-phosphate dehydrogenase

EC=1.1.5.3
Gene names
Name:glpD
Ordered Locus Names:BSU09300
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactor

FAD.

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (aerobic route): step 1/1.

Subcellular location

Cytoplasm.

Induction

Requires glycerol 3-phosphate and the GlpP product; repressed by glucose. Ref.5

Sequence similarities

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processGlycerol metabolism
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycerol catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

glycerol-3-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentglycerol-3-phosphate dehydrogenase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionsn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555Aerobic glycerol-3-phosphate dehydrogenase
PRO_0000126097

Regions

Nucleotide binding24 – 5229FAD Potential

Experimental info

Sequence conflict385 – 3862EH → DD in AAA22487. Ref.1
Sequence conflict385 – 3862EH → DD in CAA74430. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P18158 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: A4181ACBC7E7F87A

FASTA55562,568
        10         20         30         40         50         60 
MMNHQFSSLE RDRMLTDMTK KTYDLFIIGG GITGAGTALD AASRGMKVAL SEMQDFAAGT 

        70         80         90        100        110        120 
SSRSTKLVHG GLRYLKQFEV KMVAEVGKER AIVYENGPHV TTPEWMLLPF HKGGTFGSFT 

       130        140        150        160        170        180 
TSIGLRVYDF LAGVKKSERR SMLSAKETLQ KEPLVKKDGL KGGGYYVEYR TDDARLTIEV 

       190        200        210        220        230        240 
MKEAVKFGAE PVNYSKVKEL LYEKGKAVGV LIEDVLTKKE YKVYAKKIVN ATGPWVDQLR 

       250        260        270        280        290        300 
EKDHSKNGKH LQHTKGIHLV FDQSVFPLKQ AVYFDTPDGR MVFAIPREGK TYVGTTDTVY 

       310        320        330        340        350        360 
KEALEHPRMT TEDRDYVIKS INYMFPELNI TANDIESSWA GLRPLIHEEG KDPSEISRKD 

       370        380        390        400        410        420 
EIWTSDSGLI TIAGGKLTGY RKMAEHIVDL VRDRLKEEGE KDFGPCKTKN MPISGGHVGG 

       430        440        450        460        470        480 
SKNLMSFVTA KTKEGIAAGL SEKDAKQLAI RYGSNVDRVF DRVEALKDEA AKRNIPVHIL 

       490        500        510        520        530        540 
AEAEYSIEEE MTATPADFFV RRTGRLFFDI NWVRTYKDAV IDFMSERFQW DEQAKNKHTE 

       550 
NLNKLLHDAV VPLEQ 

« Hide

References

« Hide 'large scale' references
[1]"Glycerol catabolism in Bacillus subtilis: nucleotide sequence of the genes encoding glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD)."
Holmberg C., Beijer L., Rutberg B., Rutberg L.
J. Gen. Microbiol. 136:2367-2375(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene."
Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.
Microbiology 144:859-875(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 385-386.
[5]"Expression of the gene encoding glycerol-3-phosphate dehydrogenase (glpD) in Bacillus subtilis is controlled by antitermination."
Holmberg C., Rutberg B.
Mol. Microbiol. 5:2891-2900(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: TRANSCRIPTIONAL REGULATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34393 Genomic DNA. Translation: AAA22487.1.
Y14079 Genomic DNA. Translation: CAA74430.1.
AL009126 Genomic DNA. Translation: CAB12758.2.
PIRC45868.
RefSeqNP_388811.2. NC_000964.3.

3D structure databases

ProteinModelPortalP18158.
SMRP18158. Positions 6-555.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU09300.

Proteomic databases

PaxDbP18158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12758; CAB12758; BSU09300.
GeneID936250.
KEGGbsu:BSU09300.
PATRIC18973556. VBIBacSub10457_0972.

Organism-specific databases

GenoListBSU09300. [Micado]

Phylogenomic databases

eggNOGCOG0578.
HOGENOMHOG000004812.
KOK00111.
OrthoDBEOG651SR7.
PhylomeDBP18158.

Enzyme and pathway databases

BioCycBSUB:BSU09300-MONOMER.
UniPathwayUPA00618; UER00674.

Family and domain databases

InterProIPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamPF01266. DAO. 1 hit.
[Graphical view]
PRINTSPR01001. FADG3PDH.
PROSITEPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPD_BACSU
AccessionPrimary (citable) accession number: P18158
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: June 16, 2009
Last modified: July 9, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList