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Reviewed, UniProtKB/Swiss-Prot P18157 (GLPK_BACSU)

Last modified November 3, 2009. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK
Gene names
Name: glpK
Ordered Locus Names: BSU09290
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186

Enzyme regulation

Inhibited by fructose 1,6-biphosphate (FBP). HAMAP MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186

Subunit structure

Homotetramer By similarity.

Induction

Requires glycerol 3-phosphate and the glpP product; repressed by glucose. HAMAP MF_00186

Post-translational modification

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation of His-230, which leads to the activation of the enzyme. HAMAP MF_00186

Sequence similarities

Belongs to the FGGY kinase family.

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Ontologies

Keywords
   Biological processGlycerol metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycerol-3-phosphate metabolic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

glycerol kinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Glycerol kinase HAMAP MF_00186
PRO_0000059434

Regions

Nucleotide binding410 – 4145ATP By similarity

Sites

Binding site121Substrate By similarity
Binding site161ATP By similarity
Binding site821Substrate By similarity
Binding site1341Substrate By similarity
Binding site2441Substrate By similarity
Binding site2661ATP By similarity
Binding site3091ATP; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue2301Phosphohistidine; by HPr By similarity

Experimental info

Mutagenesis2301H → R: Increased activity.
Mutagenesis2321F → S: Increased activity.
Sequence conflict2011E → Q in AAA22486. Ref.1
Sequence conflict2011E → Q in CAA74429. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P18157-1 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: BBEADD9ED28C609E

FASTA49655,080
        10         20         30         40         50         60 
METYILSLDQ GTTSSRAILF NKEGKIVHSA QKEFTQYFPH PGWVEHNANE IWGSVLAVIA 

        70         80         90        100        110        120 
SVISESGISA SQIAGIGITN QRETTVVWDK DTGSPVYNAI VWQSRQTSGI CEELREKGYN 

       130        140        150        160        170        180 
DKFREKTGLL IDPYFSGTKV KWILDNVEGA REKAEKGELL FGTIDTWLIW KMSGGKAHVT 

       190        200        210        220        230        240 
DYSNASRTLM FNIYDLKWDD ELLDILGVPK SMLPEVKPSS HVYAETVDYH FFGKNIPIAG 

       250        260        270        280        290        300 
AAGDQQSALF GQACFEEGMG KNTYGTGCFM LMNTGEKAIK SEHGLLTTIA WGIDGKVNYA 

       310        320        330        340        350        360 
LEGSIFVAGS AIQWLRDGLR MFQDSSLSES YAEKVDSTDG VYVVPAFVGL GTPYWDSDVR 

       370        380        390        400        410        420 
GSVFGLTRGT TKEHFIRATL ESLAYQTKDV LDAMEADSNI SLKTLRVDGG AVKNNFLMQF 

       430        440        450        460        470        480 
QGDLLNVPVE RPEINETTAL GAAYLAGIAV GFWKDRSEIA NQWNLDKRFE PELEEEKRNE 

       490 
LYKGWQKAVK AAMAFK

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References

« Hide 'large scale' references
[1]"Glycerol catabolism in Bacillus subtilis: nucleotide sequence of the genes encoding glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD)."
Holmberg C., Beijer L., Rutberg B., Rutberg L.
J. Gen. Microbiol. 136:2367-2375(1990) [PubMed: 2127799] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene."
Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.
Microbiology 144:859-875(1998) [PubMed: 9579061] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 201.
[5]"The glpP and glpF genes of the glycerol regulon in Bacillus subtilis."
Beijer L., Nilsson R.-P., Holmberg C., Rutberg L.
J. Gen. Microbiol. 139:349-359(1993) [PubMed: 8436953] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
[6]"Mutations in the glycerol kinase gene restore the ability of a ptsGHI mutant of Bacillus subtilis to grow on glycerol."
Wehtje C., Beijer L., Nilsson R.P., Rutberg B.
Microbiology 141:1193-1198(1995) [PubMed: 7773413] [Abstract]
Cited for: MUTAGENESIS.

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Cross-references

Sequence databases

M34393 Genomic DNA. Translation: AAA22486.1.
Y14079 Genomic DNA. Translation: CAA74429.1.
AL009126 Genomic DNA. Translation: CAB12757.2.
M99611 Genomic DNA. Translation: AAA22491.1.
PIRB45868.
RefSeqNP_388810.2.

3D structure databases

HSSPHSSP built from PDB template 1GLC based on UniProtKB P08859.
ModBaseSearch...

Genome annotation databases

GeneID939741.
GenomeReviewsGene locus BSU09290 in contig AL009126_GR.
KEGGbsu:BSU09290.
NMPDRfig|224308.1.peg.929.

Organism-specific databases

SubtiListBG10187. glpK. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP18157.
OMARQTSGIC.

Enzyme and pathway databases

BioCycBSUB224308:BSU0929-MON.
BRENDA2.7.1.30. 150.

Family and domain databases

HAMAPMF_00186.
[Tree]
InterProIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PANTHERPTHR10196. FGGY_kin. 1 hit.
PTHR10196:SF9. Glycerol_kin. 1 hit.
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameGLPK_BACSU
AccessionPrimary (citable) accession number: P18157
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: June 16, 2009
Last modified: November 3, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents