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Protein

Glycerol uptake facilitator protein

Gene

glpF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Glycerol metabolism, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU09280-MONOMER.

Protein family/group databases

TCDBi1.A.8.2.1. the major intrinsic protein (mip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol uptake facilitator protein
Gene namesi
Name:glpF
Ordered Locus Names:BSU09280
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalSequence analysisAdd
BLAST
Transmembranei38 – 5821HelicalSequence analysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Transmembranei131 – 15121HelicalSequence analysisAdd
BLAST
Transmembranei164 – 18421HelicalSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Glycerol uptake facilitator proteinPRO_0000064077Add
BLAST

Proteomic databases

PaxDbiP18156.

Expressioni

Inductioni

Requires glycerol 3-phosphate and the GlpP product; repressed by glucose.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005123.

Structurei

3D structure databases

ProteinModelPortaliP18156.
SMRiP18156. Positions 1-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 663NPA 1
Motifi185 – 1873NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0580. LUCA.
HOGENOMiHOG000288285.
InParanoidiP18156.
KOiK02440.
OMAiYMIGYGH.
PhylomeDBiP18156.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAFWGEVIG TMLLIIFGAG VCAGVNLKKS LSFQSGWIVV VFGWGLGVAM
60 70 80 90 100
AAYAVGGISG AHLNPALTIA LAFVGDFPWK EVPVYIAAQM IGAIIGAVII
110 120 130 140 150
YLHYLPHWKS TDDPAAKLGV FSTGPSIPHT FANVLSEVIG TFVLVLGILA
160 170 180 190 200
IGANQFTEGL NPLIVGFLIV AIGISLGGTT GYAINPARDL GPRIAHAFLP
210 220 230 240 250
IPGKGSSNWK YAWVPVVGPI LGGSFGGVFY NAAFKGHITS SFWIVSVILV
260 270
VVLLGLYVYT KSHSAKTLSN SKYI
Length:274
Mass (Da):28,735
Last modified:April 1, 1993 - v2
Checksum:i8624D0046A63075D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99611 Genomic DNA. Translation: AAA22490.1.
Y14079 Genomic DNA. Translation: CAA74428.1.
AL009126 Genomic DNA. Translation: CAB12756.1.
M34393 Genomic DNA. Translation: AAA22485.1. Sequence problems.
PIRiC47700.
RefSeqiNP_388809.1. NC_000964.3.
WP_003233386.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB12756; CAB12756; BSU09280.
GeneIDi939266.
KEGGibsu:BSU09280.
PATRICi18973552. VBIBacSub10457_0970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99611 Genomic DNA. Translation: AAA22490.1.
Y14079 Genomic DNA. Translation: CAA74428.1.
AL009126 Genomic DNA. Translation: CAB12756.1.
M34393 Genomic DNA. Translation: AAA22485.1. Sequence problems.
PIRiC47700.
RefSeqiNP_388809.1. NC_000964.3.
WP_003233386.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP18156.
SMRiP18156. Positions 1-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005123.

Protein family/group databases

TCDBi1.A.8.2.1. the major intrinsic protein (mip) family.

Proteomic databases

PaxDbiP18156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12756; CAB12756; BSU09280.
GeneIDi939266.
KEGGibsu:BSU09280.
PATRICi18973552. VBIBacSub10457_0970.

Phylogenomic databases

eggNOGiCOG0580. LUCA.
HOGENOMiHOG000288285.
InParanoidiP18156.
KOiK02440.
OMAiYMIGYGH.
PhylomeDBiP18156.

Enzyme and pathway databases

BioCyciBSUB:BSU09280-MONOMER.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPF_BACSU
AccessioniPrimary (citable) accession number: P18156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: April 1, 1993
Last modified: September 7, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.