P18155 (MTDC_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 114.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 350 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | 5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. |
| Cofactor | Magnesium. |
| Subunit structure | Homodimer. |
| Subcellular location | |
| Miscellaneous | This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD. |
| Sequence similarities | Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | Magnesium NAD |
| Molecular function | Hydrolase Oxidoreductase |
| PTM | Acetylation |
| Technical term | Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome |
| Gene Ontology (GO) | |
| Biological process | folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrion Inferred from direct assay. Source: MGI |
| Molecular function | methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: EC methylenetetrahydrofolate dehydrogenase (NAD+) activityInferred from electronic annotation. Source: EC nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 35 | 35 | Mitochondrion Ref.1 | ||||||
| Chain | 36 – 350 | 315 | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial | PRO_0000034050 | |||||
Amino acid modifications | |||||||||
| Modified residue | 50 | 1 | N6-acetyllysine By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation and characterization of cDNA clones encoding the murine NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase." Belanger C., Mackenzie R.E. J. Biol. Chem. 264:4837-4843(1989) [PubMed: 2647744] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 36-55. |
| [2] | "Structural organization of the murine gene encoding NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase." Belanger C., Mackenzie R.E. Gene 97:283-288(1991) [PubMed: 1999293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland. |
| [5] | "Analysis of the promoter region of the gene encoding NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase." Belanger C., Peri K.G., Mackenzie R.E. Nucleic Acids Res. 19:4341-4345(1991) [PubMed: 1843253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | J04627 mRNA. Translation: AAA39827.1. M63445 M63444 Genomic DNA. Translation: AAA39828.1.AK076019 mRNA. Translation: BAC36124.1. AK159680 mRNA. Translation: BAE35283.1. AK165840 mRNA. Translation: BAE38407.1. BC019511 mRNA. Translation: AAH19511.1. S52980 Genomic DNA. No translation available. |
| IPI | IPI00109824. |
| PIR | A33267. |
| RefSeq | NP_032664.1. NM_008638.2. |
| UniGene | Mm.443. Mm.475021. |
3D structure databases | |
| ProteinModelPortal | P18155. |
| SMR | P18155. Positions 37-336. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P18155. |
PTM databases | |
| PhosphoSite | P18155. |
Proteomic databases | |
| PRIDE | P18155. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000005810; ENSMUSP00000005810; ENSMUSG00000005667. |
| GeneID | 17768. |
| KEGG | mmu:17768. |
| NMPDR | fig|10090.3.peg.14707. |
| UCSC | uc009cne.2. mouse. |
Organism-specific databases | |
| CTD | 10797. |
| MGI | MGI:1338850. Mthfd2. |
Phylogenomic databases | |
| eggNOG | roNOG07067. |
| HOGENOM | HBG328751. |
| HOVERGEN | HBG006411. |
| InParanoid | P18155. |
| OMA | NQDERVD. |
| OrthoDB | EOG4N5VZ4. |
| PhylomeDB | P18155. |
Enzyme and pathway databases | |
| BRENDA | 1.5.1.15. 3474. |
Gene expression databases | |
| ArrayExpress | P18155. |
| Bgee | P18155. |
| Genevestigator | P18155. |
| GermOnline | ENSMUSG00000005667. Mus musculus. |
Family and domain databases | |
| InterPro | IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020867. THF_DH/CycHdrlase_CS. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K13403. |
| Pfam | PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view] |
| PRINTS | PR00085. THFDHDRGNASE. |
| PROSITE | PS00766. THF_DHG_CYH_1. 1 hit. PS00767. THF_DHG_CYH_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 292477. |
| SOURCE | Search... |
Entry information
| Entry name | MTDC_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P18155 Secondary accession number(s): Q3TMN4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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