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Protein

Early growth response protein 1

Gene

EGR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri338 – 36225C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri368 – 39023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri396 – 41823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. double-stranded DNA binding Source: Ensembl
  3. histone acetyltransferase binding Source: BHF-UCL
  4. metal ion binding Source: UniProtKB-KW
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  6. RNA polymerase II core promoter sequence-specific DNA binding Source: Ensembl
  7. sequence-specific DNA binding Source: UniProtKB
  8. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  9. transcription regulatory region sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  1. BMP signaling pathway Source: Ensembl
  2. cellular response to antibiotic Source: Ensembl
  3. cellular response to cAMP Source: GO_Central
  4. cellular response to drug Source: Ensembl
  5. cellular response to follicle-stimulating hormone stimulus Source: Ensembl
  6. cellular response to gamma radiation Source: Ensembl
  7. cellular response to gonadotropin stimulus Source: GO_Central
  8. cellular response to growth factor stimulus Source: Ensembl
  9. cellular response to heparin Source: UniProtKB
  10. cellular response to hyperoxia Source: Ensembl
  11. cellular response to hypoxia Source: Ensembl
  12. cellular response to insulin stimulus Source: Ensembl
  13. cellular response to isoquinoline alkaloid Source: Ensembl
  14. cellular response to mechanical stimulus Source: Ensembl
  15. cellular response to mycophenolic acid Source: UniProtKB
  16. cellular response to steroid hormone stimulus Source: Ensembl
  17. circadian rhythm Source: Ensembl
  18. cytokine-mediated signaling pathway Source: Reactome
  19. glomerular mesangial cell proliferation Source: UniProtKB
  20. interleukin-1-mediated signaling pathway Source: BHF-UCL
  21. learning or memory Source: Ensembl
  22. long-term synaptic potentiation Source: Ensembl
  23. negative regulation of apoptotic process Source: GO_Central
  24. negative regulation of canonical Wnt signaling pathway Source: Ensembl
  25. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  26. oligodendrocyte differentiation Source: Ensembl
  27. positive regulation of glomerular metanephric mesangial cell proliferation Source: UniProtKB
  28. positive regulation of neuron apoptotic process Source: Ensembl
  29. positive regulation of transcription, DNA-templated Source: UniProtKB
  30. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  31. regulation of long-term neuronal synaptic plasticity Source: GO_Central
  32. regulation of protein sumoylation Source: BHF-UCL
  33. response to amphetamine Source: Ensembl
  34. response to carbon monoxide Source: Ensembl
  35. response to cocaine Source: Ensembl
  36. response to electrical stimulus Source: Ensembl
  37. response to ethanol Source: Ensembl
  38. response to glucose Source: Ensembl
  39. response to norepinephrine Source: Ensembl
  40. response to nutrient levels Source: Ensembl
  41. skeletal muscle cell differentiation Source: GO_Central
  42. T cell differentiation Source: Ensembl
  43. transcription from RNA polymerase II promoter Source: BHF-UCL
  44. type I interferon signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_25162. Interferon alpha/beta signaling.
SignaLinkiP18146.

Names & Taxonomyi

Protein namesi
Recommended name:
Early growth response protein 1
Short name:
EGR-1
Alternative name(s):
AT225
Nerve growth factor-induced protein A
Short name:
NGFI-A
Transcription factor ETR103
Transcription factor Zif268
Zinc finger protein 225
Zinc finger protein Krox-24
Gene namesi
Name:EGR1
Synonyms:KROX24, ZNF225
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:3238. EGR1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleoplasm Source: HPA
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27673.

Polymorphism and mutation databases

BioMutaiEGR1.
DMDMi119242.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Early growth response protein 1PRO_0000047109Add
BLAST

Proteomic databases

PaxDbiP18146.
PRIDEiP18146.

PTM databases

PhosphoSiteiP18146.

Expressioni

Inductioni

By growth factors.

Gene expression databases

BgeeiP18146.
CleanExiHS_EGR1.
HS_ZNF225.
ExpressionAtlasiP18146. baseline and differential.
GenevestigatoriP18146.

Organism-specific databases

HPAiCAB019427.
HPA029938.

Interactioni

Subunit structurei

Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CDKN2AQ8N7264EBI-2834611,EBI-625922
CTNNB1P352227EBI-2834611,EBI-491549

Protein-protein interaction databases

BioGridi108278. 23 interactions.
IntActiP18146. 13 interactions.
MINTiMINT-234136.
STRINGi9606.ENSP00000239938.

Structurei

Secondary structure

1
543
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi348 – 3514Combined sources
Helixi352 – 36312Combined sources
Turni371 – 3733Combined sources
Beta strandi376 – 3783Combined sources
Helixi380 – 39112Combined sources
Turni399 – 4013Combined sources
Beta strandi404 – 4074Combined sources
Helixi408 – 4158Combined sources
Helixi416 – 4183Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4R2AX-ray1.59A335-423[»]
4R2CX-ray1.89A335-423[»]
4R2DX-ray2.09A335-423[»]
ProteinModelPortaliP18146.
SMRiP18146. Positions 336-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi57 – 8428Gly/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri338 – 36225C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri368 – 39023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri396 – 41823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP18146.
KOiK09203.
OMAiAFATQTG.
OrthoDBiEOG7Z69C7.
PhylomeDBiP18146.
TreeFamiTF318980.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 2 hits.
PF11928. DUF3446. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG
60 70 80 90 100
AAGAPEGSGS NSSSSSSGGG GGGGGGSNSS SSSSTFNPQA DTGEQPYEHL
110 120 130 140 150
TAESFPDISL NNEKVLVETS YPSQTTRLPP ITYTGRFSLE PAPNSGNTLW
160 170 180 190 200
PEPLFSLVSG LVSMTNPPAS SSSAPSPAAS SASASQSPPL SCAVPSNDSS
210 220 230 240 250
PIYSAAPTFP TPNTDIFPEP QSQAFPGSAG TALQYPPPAY PAAKGGFQVP
260 270 280 290 300
MIPDYLFPQQ QGDLGLGTPD QKPFQGLESR TQQPSLTPLS TIKAFATQSG
310 320 330 340 350
SQDLKALNTS YQSQLIKPSR MRKYPNRPSK TPPHERPYAC PVESCDRRFS
360 370 380 390 400
RSDELTRHIR IHTGQKPFQC RICMRNFSRS DHLTTHIRTH TGEKPFACDI
410 420 430 440 450
CGRKFARSDE RKRHTKIHLR QKDKKADKSV VASSATSSLS SYPSPVATSY
460 470 480 490 500
PSPVTTSYPS PATTSYPSPV PTSFSSPGSS TYPSPVHSGF PSPSVATTYS
510 520 530 540
SVPPAFPAQV SSFPSSAVTN SFSASTGLSD MTATFSPRTI EIC
Length:543
Mass (Da):57,507
Last modified:November 1, 1990 - v1
Checksum:i768CE670D9743E4E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti28 – 281T → I.
Corresponds to variant rs13181973 [ dbSNP | Ensembl ].
VAR_052712
Natural varianti144 – 1441N → K.
Corresponds to variant rs28365166 [ dbSNP | Ensembl ].
VAR_029330
Natural varianti145 – 1451S → R.
Corresponds to variant rs28365164 [ dbSNP | Ensembl ].
VAR_029331
Natural varianti219 – 2191E → D.
Corresponds to variant rs28365165 [ dbSNP | Ensembl ].
VAR_029332

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52541 mRNA. Translation: CAA36777.1.
M62829 mRNA. Translation: AAA35815.1.
M80583 mRNA. No translation available.
BC073983 mRNA. Translation: AAH73983.1.
CCDSiCCDS4206.1.
PIRiA41211.
RefSeqiNP_001955.1. NM_001964.2.
UniGeneiHs.326035.
Hs.708393.

Genome annotation databases

EnsembliENST00000239938; ENSP00000239938; ENSG00000120738.
GeneIDi1958.
KEGGihsa:1958.
UCSCiuc003ldb.1. human.

Polymorphism and mutation databases

BioMutaiEGR1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52541 mRNA. Translation: CAA36777.1.
M62829 mRNA. Translation: AAA35815.1.
M80583 mRNA. No translation available.
BC073983 mRNA. Translation: AAH73983.1.
CCDSiCCDS4206.1.
PIRiA41211.
RefSeqiNP_001955.1. NM_001964.2.
UniGeneiHs.326035.
Hs.708393.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4R2AX-ray1.59A335-423[»]
4R2CX-ray1.89A335-423[»]
4R2DX-ray2.09A335-423[»]
ProteinModelPortaliP18146.
SMRiP18146. Positions 336-420.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108278. 23 interactions.
IntActiP18146. 13 interactions.
MINTiMINT-234136.
STRINGi9606.ENSP00000239938.

PTM databases

PhosphoSiteiP18146.

Polymorphism and mutation databases

BioMutaiEGR1.
DMDMi119242.

Proteomic databases

PaxDbiP18146.
PRIDEiP18146.

Protocols and materials databases

DNASUi1958.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000239938; ENSP00000239938; ENSG00000120738.
GeneIDi1958.
KEGGihsa:1958.
UCSCiuc003ldb.1. human.

Organism-specific databases

CTDi1958.
GeneCardsiGC05P137801.
HGNCiHGNC:3238. EGR1.
HPAiCAB019427.
HPA029938.
MIMi128990. gene.
neXtProtiNX_P18146.
PharmGKBiPA27673.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP18146.
KOiK09203.
OMAiAFATQTG.
OrthoDBiEOG7Z69C7.
PhylomeDBiP18146.
TreeFamiTF318980.

Enzyme and pathway databases

ReactomeiREACT_25162. Interferon alpha/beta signaling.
SignaLinkiP18146.

Miscellaneous databases

ChiTaRSiEGR1. human.
GeneWikiiEGR1.
GenomeRNAii1958.
NextBioi7943.
PROiP18146.
SOURCEiSearch...

Gene expression databases

BgeeiP18146.
CleanExiHS_EGR1.
HS_ZNF225.
ExpressionAtlasiP18146. baseline and differential.
GenevestigatoriP18146.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 2 hits.
PF11928. DUF3446. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence of the human cellular early growth response gene Egr-1."
    Suggs S.V., Katzowitz J.L., Tsai-Morris C.-H., Sukhatme V.P.
    Nucleic Acids Res. 18:4283-4283(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Foreskin.
  2. "A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation."
    Shimizu N., Ohta M., Fujiwara C., Sagara J., Mochizuki N., Oda T., Utiyama H.
    J. Biochem. 111:272-277(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Expression of a zinc finger gene in HTLV-I- and HTLV-II-transformed cells."
    Wright J.J., Gunter K.C., Mitsuya H., Irving S.G., Kelly K., Siebenlist U.
    Science 248:588-591(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: PNS.
  5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  6. "Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b."
    Hu C.T., Chang T.Y., Cheng C.C., Liu C.S., Wu J.R., Li M.C., Wu W.S.
    FEBS J. 277:1202-1218(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNAI1 AND SP1.

Entry informationi

Entry nameiEGR1_HUMAN
AccessioniPrimary (citable) accession number: P18146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 29, 2015
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.