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P18146 (EGR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Early growth response protein 1

Short name=EGR-1
Alternative name(s):
AT225
Nerve growth factor-induced protein A
Short name=NGFI-A
Transcription factor ETR103
Transcription factor Zif268
Zinc finger protein 225
Zinc finger protein Krox-24
Gene names
Name:EGR1
Synonyms:KROX24, ZNF225
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Subunit structure

Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction. Ref.6

Subcellular location

Nucleus.

Induction

By growth factors.

Sequence similarities

Belongs to the EGR C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

T cell differentiation

Inferred from electronic annotation. Source: Ensembl

cellular response to antibiotic

Inferred from electronic annotation. Source: Ensembl

cellular response to cAMP

Inferred from Biological aspect of Ancestor. Source: RefGenome

cellular response to drug

Inferred from electronic annotation. Source: Ensembl

cellular response to follicle-stimulating hormone stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to gamma radiation

Inferred from electronic annotation. Source: Ensembl

cellular response to gonadotropin stimulus

Inferred from Biological aspect of Ancestor. Source: RefGenome

cellular response to growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to heparin

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to hyperoxia

Inferred from electronic annotation. Source: Ensembl

cellular response to hypoxia

Inferred from electronic annotation. Source: Ensembl

cellular response to insulin stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to isoquinoline alkaloid

Inferred from electronic annotation. Source: Ensembl

cellular response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to mycophenolic acid

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to steroid hormone stimulus

Inferred from electronic annotation. Source: Ensembl

circadian rhythm

Inferred from electronic annotation. Source: Ensembl

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

glomerular mesangial cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

interleukin-1-mediated signaling pathway

Inferred from mutant phenotype PubMed 20018936. Source: BHF-UCL

learning or memory

Inferred from electronic annotation. Source: Ensembl

long-term synaptic potentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of apoptotic process

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

oligodendrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of glomerular metanephric mesangial cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19057511. Source: BHF-UCL

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 12560508. Source: UniProtKB

regulation of long-term neuronal synaptic plasticity

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of protein sumoylation

Inferred from direct assay PubMed 19057511. Source: BHF-UCL

response to amphetamine

Inferred from electronic annotation. Source: Ensembl

response to carbon monoxide

Inferred from electronic annotation. Source: Ensembl

response to cocaine

Inferred from electronic annotation. Source: Ensembl

response to electrical stimulus

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to glucose

Inferred from electronic annotation. Source: Ensembl

response to norepinephrine

Inferred from electronic annotation. Source: Ensembl

response to nutrient levels

Inferred from electronic annotation. Source: Ensembl

skeletal muscle cell differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19057511. Source: BHF-UCL

type I interferon signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12560508. Source: UniProtKB

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II core promoter sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

histone acetyltransferase binding

Inferred from physical interaction PubMed 20018936. Source: BHF-UCL

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding

Inferred from direct assay PubMed 1662794. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 12560508. Source: UniProtKB

transcription regulatory region sequence-specific DNA binding

Inferred from direct assay PubMed 18718911. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDKN2AQ8N7264EBI-2834611,EBI-625922
CTNNB1P352227EBI-2834611,EBI-491549

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 543543Early growth response protein 1
PRO_0000047109

Regions

Zinc finger338 – 36225C2H2-type 1
Zinc finger368 – 39023C2H2-type 2
Zinc finger396 – 41823C2H2-type 3
Compositional bias57 – 8428Gly/Ser-rich

Natural variations

Natural variant281T → I.
Corresponds to variant rs13181973 [ dbSNP | Ensembl ].
VAR_052712
Natural variant1441N → K.
Corresponds to variant rs28365166 [ dbSNP | Ensembl ].
VAR_029330
Natural variant1451S → R.
Corresponds to variant rs28365164 [ dbSNP | Ensembl ].
VAR_029331
Natural variant2191E → D.
Corresponds to variant rs28365165 [ dbSNP | Ensembl ].
VAR_029332

Sequences

Sequence LengthMass (Da)Tools
P18146 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 768CE670D9743E4E

FASTA54357,507
        10         20         30         40         50         60 
MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG AAGAPEGSGS 

        70         80         90        100        110        120 
NSSSSSSGGG GGGGGGSNSS SSSSTFNPQA DTGEQPYEHL TAESFPDISL NNEKVLVETS 

       130        140        150        160        170        180 
YPSQTTRLPP ITYTGRFSLE PAPNSGNTLW PEPLFSLVSG LVSMTNPPAS SSSAPSPAAS 

       190        200        210        220        230        240 
SASASQSPPL SCAVPSNDSS PIYSAAPTFP TPNTDIFPEP QSQAFPGSAG TALQYPPPAY 

       250        260        270        280        290        300 
PAAKGGFQVP MIPDYLFPQQ QGDLGLGTPD QKPFQGLESR TQQPSLTPLS TIKAFATQSG 

       310        320        330        340        350        360 
SQDLKALNTS YQSQLIKPSR MRKYPNRPSK TPPHERPYAC PVESCDRRFS RSDELTRHIR 

       370        380        390        400        410        420 
IHTGQKPFQC RICMRNFSRS DHLTTHIRTH TGEKPFACDI CGRKFARSDE RKRHTKIHLR 

       430        440        450        460        470        480 
QKDKKADKSV VASSATSSLS SYPSPVATSY PSPVTTSYPS PATTSYPSPV PTSFSSPGSS 

       490        500        510        520        530        540 
TYPSPVHSGF PSPSVATTYS SVPPAFPAQV SSFPSSAVTN SFSASTGLSD MTATFSPRTI 


EIC 

« Hide

References

« Hide 'large scale' references
[1]"cDNA sequence of the human cellular early growth response gene Egr-1."
Suggs S.V., Katzowitz J.L., Tsai-Morris C.-H., Sukhatme V.P.
Nucleic Acids Res. 18:4283-4283(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Foreskin.
[2]"A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation."
Shimizu N., Ohta M., Fujiwara C., Sagara J., Mochizuki N., Oda T., Utiyama H.
J. Biochem. 111:272-277(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Expression of a zinc finger gene in HTLV-I- and HTLV-II-transformed cells."
Wright J.J., Gunter K.C., Mitsuya H., Irving S.G., Kelly K., Siebenlist U.
Science 248:588-591(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: PNS.
[5]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[6]"Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b."
Hu C.T., Chang T.Y., Cheng C.C., Liu C.S., Wu J.R., Li M.C., Wu W.S.
FEBS J. 277:1202-1218(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNAI1 AND SP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52541 mRNA. Translation: CAA36777.1.
M62829 mRNA. Translation: AAA35815.1.
M80583 mRNA. No translation available.
BC073983 mRNA. Translation: AAH73983.1.
PIRA41211.
RefSeqNP_001955.1. NM_001964.2.
UniGeneHs.326035.
Hs.708393.

3D structure databases

ProteinModelPortalP18146.
SMRP18146. Positions 336-443.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108278. 23 interactions.
IntActP18146. 13 interactions.
MINTMINT-234136.
STRING9606.ENSP00000239938.

PTM databases

PhosphoSiteP18146.

Polymorphism databases

DMDM119242.

Proteomic databases

PaxDbP18146.
PRIDEP18146.

Protocols and materials databases

DNASU1958.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000239938; ENSP00000239938; ENSG00000120738.
GeneID1958.
KEGGhsa:1958.
UCSCuc003ldb.1. human.

Organism-specific databases

CTD1958.
GeneCardsGC05P137801.
HGNCHGNC:3238. EGR1.
HPACAB019427.
HPA029938.
MIM128990. gene.
neXtProtNX_P18146.
PharmGKBPA27673.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000036856.
HOVERGENHBG003909.
InParanoidP18146.
KOK09203.
OMAAVTNSFS.
OrthoDBEOG7Z69C7.
PhylomeDBP18146.
TreeFamTF318980.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
SignaLinkP18146.

Gene expression databases

ArrayExpressP18146.
BgeeP18146.
CleanExHS_EGR1.
HS_ZNF225.
GenevestigatorP18146.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF11914. DUF3432. 2 hits.
PF11928. DUF3446. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEGR1. human.
GeneWikiEGR1.
GenomeRNAi1958.
NextBio7943.
PROP18146.
SOURCESearch...

Entry information

Entry nameEGR1_HUMAN
AccessionPrimary (citable) accession number: P18146
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM