P18146 (EGR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Early growth response protein 1
Short name=EGR-1

Alternative name(s):
AT225
Nerve growth factor-induced protein A
Short name=NGFI-A
Transcription factor ETR103
Transcription factor Zif268
Zinc finger protein 225
Zinc finger protein Krox-24

Gene names

Name:	EGR1
Synonyms:	KROX24, ZNF225

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Subunit structure	Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction. Ref.6
Subcellular location	Nucleus.
Induction	By growth factors.
Sequence similarities	Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from electronic annotation. Source: Compara T cell differentiation Inferred from electronic annotation. Source: Compara cellular response to antibiotic Inferred from electronic annotation. Source: Compara cellular response to cAMP Inferred from electronic annotation. Source: Compara cellular response to drug Inferred from electronic annotation. Source: Compara cellular response to follicle-stimulating hormone stimulus Inferred from electronic annotation. Source: Compara cellular response to gamma radiation Inferred from electronic annotation. Source: Compara cellular response to growth factor stimulus Inferred from electronic annotation. Source: Compara cellular response to heparin Inferred from sequence or structural similarity. Source: UniProtKB cellular response to hyperoxia Inferred from electronic annotation. Source: Compara cellular response to hypoxia Inferred from electronic annotation. Source: Compara cellular response to insulin stimulus Inferred from electronic annotation. Source: Compara cellular response to isoquinoline alkaloid Inferred from electronic annotation. Source: Compara cellular response to mechanical stimulus Inferred from electronic annotation. Source: Compara cellular response to mycophenolic acid Inferred from sequence or structural similarity. Source: UniProtKB cellular response to steroid hormone stimulus Inferred from electronic annotation. Source: Compara circadian rhythm Inferred from electronic annotation. Source: Compara glomerular mesangial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB interleukin-1-mediated signaling pathway Inferred from mutant phenotype PubMed 20018936. Source: BHF-UCL learning or memory Inferred from electronic annotation. Source: Compara long-term synaptic potentiation Inferred from electronic annotation. Source: Compara negative regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara oligodendrocyte differentiation Inferred from electronic annotation. Source: Compara positive regulation of glomerular metanephric mesangial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 19057511. Source: BHF-UCL regulation of long-term neuronal synaptic plasticity Inferred from electronic annotation. Source: Compara regulation of protein sumoylation Inferred from direct assay PubMed 19057511. Source: BHF-UCL response to amphetamine Inferred from electronic annotation. Source: Compara response to carbon monoxide Inferred from electronic annotation. Source: Compara response to cocaine Inferred from electronic annotation. Source: Compara response to electrical stimulus Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to glucose stimulus Inferred from electronic annotation. Source: Compara response to norepinephrine stimulus Inferred from electronic annotation. Source: Compara response to nutrient levels Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Inferred from direct assay PubMed 19057511. Source: BHF-UCL type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from electronic annotation. Source: Compara RNA polymerase II core promoter sequence-specific DNA binding Inferred from electronic annotation. Source: Compara double-stranded DNA binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 12560508. Source: UniProtKB transcription regulatory region sequence-specific DNA binding Inferred from direct assay PubMed 18718911. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTNNB1	P35222	7	EBI-2834611,EBI-491549

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Early growth response protein 1		PRO_0000047109
Regions
	Zinc finger	338 – 362	25	C2H2-type 1
	Zinc finger	368 – 390	23	C2H2-type 2
	Zinc finger	396 – 418	23	C2H2-type 3
	Compositional bias	57 – 84	28	Gly/Ser-rich
Natural variations
	Natural variant	28	1	T → I. Corresponds to variant rs13181973 [ dbSNP | Ensembl ].		VAR_052712
	Natural variant	144	1	N → K. Corresponds to variant rs28365166 [ dbSNP | Ensembl ].		VAR_029330
	Natural variant	145	1	S → R. Corresponds to variant rs28365164 [ dbSNP | Ensembl ].		VAR_029331
	Natural variant	219	1	E → D. Corresponds to variant rs28365165 [ dbSNP | Ensembl ].		VAR_029332

Sequences

	Sequence		Length	Mass (Da)
	P18146 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 768CE670D9743E4E	FASTA	543	57,507
	10 20 30 40 50 60 MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG AAGAPEGSGS 70 80 90 100 110 120 NSSSSSSGGG GGGGGGSNSS SSSSTFNPQA DTGEQPYEHL TAESFPDISL NNEKVLVETS 130 140 150 160 170 180 YPSQTTRLPP ITYTGRFSLE PAPNSGNTLW PEPLFSLVSG LVSMTNPPAS SSSAPSPAAS 190 200 210 220 230 240 SASASQSPPL SCAVPSNDSS PIYSAAPTFP TPNTDIFPEP QSQAFPGSAG TALQYPPPAY 250 260 270 280 290 300 PAAKGGFQVP MIPDYLFPQQ QGDLGLGTPD QKPFQGLESR TQQPSLTPLS TIKAFATQSG 310 320 330 340 350 360 SQDLKALNTS YQSQLIKPSR MRKYPNRPSK TPPHERPYAC PVESCDRRFS RSDELTRHIR 370 380 390 400 410 420 IHTGQKPFQC RICMRNFSRS DHLTTHIRTH TGEKPFACDI CGRKFARSDE RKRHTKIHLR 430 440 450 460 470 480 QKDKKADKSV VASSATSSLS SYPSPVATSY PSPVTTSYPS PATTSYPSPV PTSFSSPGSS 490 500 510 520 530 540 TYPSPVHSGF PSPSVATTYS SVPPAFPAQV SSFPSSAVTN SFSASTGLSD MTATFSPRTI EIC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence of the human cellular early growth response gene Egr-1." Suggs S.V., Katzowitz J.L., Tsai-Morris C.-H., Sukhatme V.P. Nucleic Acids Res. 18:4283-4283(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Foreskin.
[2]	"A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation." Shimizu N., Ohta M., Fujiwara C., Sagara J., Mochizuki N., Oda T., Utiyama H. J. Biochem. 111:272-277(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Expression of a zinc finger gene in HTLV-I- and HTLV-II-transformed cells." Wright J.J., Gunter K.C., Mitsuya H., Irving S.G., Kelly K., Siebenlist U. Science 248:588-591(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS.
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[6]	"Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b." Hu C.T., Chang T.Y., Cheng C.C., Liu C.S., Wu J.R., Li M.C., Wu W.S. FEBS J. 277:1202-1218(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SNAI1 AND SP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52541 mRNA. Translation: CAA36777.1. M62829 mRNA. Translation: AAA35815.1. M80583 mRNA. No translation available. BC073983 mRNA. Translation: AAH73983.1.
IPI	IPI00030227.
PIR	A41211.
RefSeq	NP_001955.1. NM_001964.2.
UniGene	Hs.326035. Hs.708393.
3D structure databases
ProteinModelPortal	P18146.
ModBase	Search...
Protein-protein interaction databases
IntAct	P18146. 6 interactions.
MINT	MINT-234136.
STRING	9606.ENSP00000239938.
PTM databases
PhosphoSite	P18146.
Polymorphism databases
DMDM	119242.
Proteomic databases
PaxDb	P18146.
PRIDE	P18146.
Protocols and materials databases
DNASU	1958.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000239938; ENSP00000239938; ENSG00000120738.
GeneID	1958.
KEGG	hsa:1958.
UCSC	uc003ldb.1. human.
Organism-specific databases
CTD	1958.
GeneCards	GC05P137801.
HGNC	HGNC:3238. EGR1.
HPA	CAB019427.
MIM	128990. gene.
neXtProt	NX_P18146.
PharmGKB	PA27673.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000036856.
HOVERGEN	HBG003909.
InParanoid	P18146.
KO	K09203.
OMA	PFGSFPH.
OrthoDB	EOG4BP1BT.
PhylomeDB	P18146.
Enzyme and pathway databases
Pathway_Interaction_DB	nfat_tfpathway. Calcineurin-regulated NFAT-dependent transcription in lymphocytes. cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells. ar_tf_pathway. Regulation of Androgen receptor activity. prlsignalingeventspathway. Signaling events mediated by PRL. pi3kplctrkpathway. Trk receptor signaling mediated by PI3K and PLC-gamma. mapktrkpathway. Trk receptor signaling mediated by the MAPK pathway.
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	P18146.
Bgee	P18146.
CleanEx	HS_EGR1. HS_ZNF225.
Genevestigator	P18146.
GermOnline	ENSG00000120738. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR021839. DUF3432. IPR021849. DUF3446. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF11914. DUF3432. 2 hits. PF11928. DUF3446. 1 hit. PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EGR1. human.
GenomeRNAi	1958.
NextBio	7943.
SOURCE	Search...

Entry information

Entry name

EGR1_HUMAN

Accession

Primary (citable) accession number: P18146

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.