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Protein

Early growth response protein 1

Gene

EGR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator (PubMed:20121949). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status (PubMed:25258363, PubMed:25999311). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri338 – 362C2H2-type 1PROSITE-ProRule annotationCombined sources2 PublicationsAdd BLAST25
Zinc fingeri368 – 390C2H2-type 2PROSITE-ProRule annotationCombined sources2 PublicationsAdd BLAST23
Zinc fingeri396 – 418C2H2-type 3PROSITE-ProRule annotationCombined sources2 PublicationsAdd BLAST23

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • double-stranded methylated DNA binding Source: UniProtKB
  • hemi-methylated DNA-binding Source: UniProtKB
  • histone acetyltransferase binding Source: BHF-UCL
  • promoter-specific chromatin binding Source: UniProtKB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: UniProtKB
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: Ensembl
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB
  • transcription regulatory region sequence-specific DNA binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120738-MONOMER.
ReactomeiR-HSA-909733. Interferon alpha/beta signaling.
SignaLinkiP18146.
SIGNORiP18146.

Names & Taxonomyi

Protein namesi
Recommended name:
Early growth response protein 11 Publication
Short name:
EGR-11 Publication
Alternative name(s):
AT225
Nerve growth factor-induced protein A
Short name:
NGFI-A
Transcription factor ETR103
Transcription factor Zif268
Zinc finger protein 2251 Publication
Zinc finger protein Krox-24
Gene namesi
Name:EGR1
Synonyms:KROX24, ZNF2251 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:3238. EGR1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1958.
OpenTargetsiENSG00000120738.
PharmGKBiPA27673.

Chemistry databases

ChEMBLiCHEMBL3616355.

Polymorphism and mutation databases

BioMutaiEGR1.
DMDMi119242.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471091 – 543Early growth response protein 1Add BLAST543

Proteomic databases

EPDiP18146.
PaxDbiP18146.
PeptideAtlasiP18146.
PRIDEiP18146.

PTM databases

iPTMnetiP18146.
PhosphoSitePlusiP18146.

Expressioni

Tissue specificityi

Detected in neutrophils (at protein level).1 Publication

Inductioni

By growth factors.1 Publication

Gene expression databases

BgeeiENSG00000120738.
CleanExiHS_EGR1.
HS_ZNF225.
ExpressionAtlasiP18146. baseline and differential.
GenevisibleiP18146. HS.

Organism-specific databases

HPAiCAB019427.
HPA029937.
HPA029938.

Interactioni

Subunit structurei

Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei336Interaction with DNACombined sources1 Publication1
Sitei347Interaction with DNABy similarity1
Sitei351Interaction with DNABy similarity1
Sitei357Interaction with DNACombined sources1 Publication1
Sitei375Interaction with DNABy similarity1
Sitei379Interaction with DNACombined sources1 Publication1
Sitei403Interaction with DNACombined sources1 Publication1
Sitei407Interaction with DNACombined sources1 Publication1
Sitei413Interaction with DNACombined sources2 Publications1

Binary interactionsi

WithEntry#Exp.IntActNotes
CDKN2AQ8N7264EBI-2834611,EBI-625922
CTNNB1P352227EBI-2834611,EBI-491549

GO - Molecular functioni

  • histone acetyltransferase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi108278. 36 interactors.
IntActiP18146. 23 interactors.
MINTiMINT-234136.
STRINGi9606.ENSP00000239938.

Structurei

Secondary structure

1543
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi348 – 351Combined sources4
Helixi352 – 363Combined sources12
Turni371 – 373Combined sources3
Beta strandi376 – 378Combined sources3
Helixi380 – 391Combined sources12
Turni399 – 401Combined sources3
Beta strandi404 – 407Combined sources4
Helixi408 – 415Combined sources8
Helixi416 – 418Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4R2AX-ray1.59A335-423[»]
4R2CX-ray1.89A335-423[»]
4R2DX-ray2.09A335-423[»]
4X9JX-ray1.41A335-423[»]
ProteinModelPortaliP18146.
SMRiP18146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi57 – 84Gly/Ser-richAdd BLAST28

Domaini

Binds to DNA motifs with the sequence 5'-GCG(T/G)GGGCG-3' via its C2H2-type zinc fingers (PubMed:25258363, PubMed:25999311). The first, most N-terminal zinc finger binds to the 3'-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5'-GCG motif. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Has reduced affinity for target DNA where the cytosines have been oxidized to 5-hydroxymethylcytosine. Does not bind target DNA where the cytosines have been oxidized to 5-formylcytosine or 5-carboxylcytosine (PubMed:25258363).2 Publications

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri338 – 362C2H2-type 1PROSITE-ProRule annotationCombined sources2 PublicationsAdd BLAST25
Zinc fingeri368 – 390C2H2-type 2PROSITE-ProRule annotationCombined sources2 PublicationsAdd BLAST23
Zinc fingeri396 – 418C2H2-type 3PROSITE-ProRule annotationCombined sources2 PublicationsAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00550000074455.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP18146.
KOiK09203.
OMAiFATQTGS.
OrthoDBiEOG091G06VX.
PhylomeDBiP18146.
TreeFamiTF318980.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 2 hits.
PF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAKAEMQL MSPLQISDPF GSFPHSPTMD NYPKLEEMML LSNGAPQFLG
60 70 80 90 100
AAGAPEGSGS NSSSSSSGGG GGGGGGSNSS SSSSTFNPQA DTGEQPYEHL
110 120 130 140 150
TAESFPDISL NNEKVLVETS YPSQTTRLPP ITYTGRFSLE PAPNSGNTLW
160 170 180 190 200
PEPLFSLVSG LVSMTNPPAS SSSAPSPAAS SASASQSPPL SCAVPSNDSS
210 220 230 240 250
PIYSAAPTFP TPNTDIFPEP QSQAFPGSAG TALQYPPPAY PAAKGGFQVP
260 270 280 290 300
MIPDYLFPQQ QGDLGLGTPD QKPFQGLESR TQQPSLTPLS TIKAFATQSG
310 320 330 340 350
SQDLKALNTS YQSQLIKPSR MRKYPNRPSK TPPHERPYAC PVESCDRRFS
360 370 380 390 400
RSDELTRHIR IHTGQKPFQC RICMRNFSRS DHLTTHIRTH TGEKPFACDI
410 420 430 440 450
CGRKFARSDE RKRHTKIHLR QKDKKADKSV VASSATSSLS SYPSPVATSY
460 470 480 490 500
PSPVTTSYPS PATTSYPSPV PTSFSSPGSS TYPSPVHSGF PSPSVATTYS
510 520 530 540
SVPPAFPAQV SSFPSSAVTN SFSASTGLSD MTATFSPRTI EIC
Length:543
Mass (Da):57,507
Last modified:November 1, 1990 - v1
Checksum:i768CE670D9743E4E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05271228T → I.Corresponds to variant rs13181973dbSNPEnsembl.1
Natural variantiVAR_029330144N → K.Corresponds to variant rs28365166dbSNPEnsembl.1
Natural variantiVAR_029331145S → R.Corresponds to variant rs28365164dbSNPEnsembl.1
Natural variantiVAR_029332219E → D.Corresponds to variant rs28365165dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52541 mRNA. Translation: CAA36777.1.
M62829 mRNA. Translation: AAA35815.1.
M80583 mRNA. No translation available.
BC073983 mRNA. Translation: AAH73983.1.
CCDSiCCDS4206.1.
PIRiA41211.
RefSeqiNP_001955.1. NM_001964.2.
UniGeneiHs.326035.
Hs.708393.

Genome annotation databases

EnsembliENST00000239938; ENSP00000239938; ENSG00000120738.
GeneIDi1958.
KEGGihsa:1958.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52541 mRNA. Translation: CAA36777.1.
M62829 mRNA. Translation: AAA35815.1.
M80583 mRNA. No translation available.
BC073983 mRNA. Translation: AAH73983.1.
CCDSiCCDS4206.1.
PIRiA41211.
RefSeqiNP_001955.1. NM_001964.2.
UniGeneiHs.326035.
Hs.708393.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4R2AX-ray1.59A335-423[»]
4R2CX-ray1.89A335-423[»]
4R2DX-ray2.09A335-423[»]
4X9JX-ray1.41A335-423[»]
ProteinModelPortaliP18146.
SMRiP18146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108278. 36 interactors.
IntActiP18146. 23 interactors.
MINTiMINT-234136.
STRINGi9606.ENSP00000239938.

Chemistry databases

ChEMBLiCHEMBL3616355.

PTM databases

iPTMnetiP18146.
PhosphoSitePlusiP18146.

Polymorphism and mutation databases

BioMutaiEGR1.
DMDMi119242.

Proteomic databases

EPDiP18146.
PaxDbiP18146.
PeptideAtlasiP18146.
PRIDEiP18146.

Protocols and materials databases

DNASUi1958.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000239938; ENSP00000239938; ENSG00000120738.
GeneIDi1958.
KEGGihsa:1958.

Organism-specific databases

CTDi1958.
DisGeNETi1958.
GeneCardsiEGR1.
HGNCiHGNC:3238. EGR1.
HPAiCAB019427.
HPA029937.
HPA029938.
MIMi128990. gene.
neXtProtiNX_P18146.
OpenTargetsiENSG00000120738.
PharmGKBiPA27673.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00550000074455.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP18146.
KOiK09203.
OMAiFATQTGS.
OrthoDBiEOG091G06VX.
PhylomeDBiP18146.
TreeFamiTF318980.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120738-MONOMER.
ReactomeiR-HSA-909733. Interferon alpha/beta signaling.
SignaLinkiP18146.
SIGNORiP18146.

Miscellaneous databases

ChiTaRSiEGR1. human.
GeneWikiiEGR1.
GenomeRNAii1958.
PROiP18146.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120738.
CleanExiHS_EGR1.
HS_ZNF225.
ExpressionAtlasiP18146. baseline and differential.
GenevisibleiP18146. HS.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 2 hits.
PF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEGR1_HUMAN
AccessioniPrimary (citable) accession number: P18146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 30, 2016
This is version 168 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.