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P18134

- HPRT_VIBHA

UniProt

P18134 - HPRT_VIBHA

Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    • Comment

    Functioni

    Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei102 – 1021Proton acceptorBy similarity
    Binding sitei130 – 1301IMPBy similarity
    Binding sitei152 – 1521IMP; via carbonyl oxygenBy similarity
    Metal bindingi158 – 1581MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi98 – 10710IMPBy similarity
    Nucleotide bindingi157 – 1582IMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. IMP salvage Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-3881-MONOMER.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HPRT
    Gene namesi
    Name:hpt
    OrganismiVibrio harveyi (Beneckea harveyi)
    Taxonomic identifieri669 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 176176Hypoxanthine phosphoribosyltransferasePRO_0000139637Add
    BLAST

    Proteomic databases

    PRIDEiP18134.

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliP18134.
    SMRiP18134. Positions 1-173.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18134-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKHTVEVMIS EQEVQERIRE LGKQITERYQ GSEDLVMVGL LRGSFVFMAD    50
    LARAIELTHQ VDFMTASSYG NTMESSRDVR ILKDLDDDIK GKDVLIVEDI 100
    IDTGNTLNKI REILSLREPK SIAICTLLDK PSRREVEVPV DYVGFAIPDE 150
    FVVGVGIDYA QKYRDLPFIG KVVPQE 176
    Length:176
    Mass (Da):19,963
    Last modified:November 1, 1990 - v1
    Checksum:i2027D73A9CAAAACE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53382 Genomic DNA. Translation: CAA37462.1.
    PIRiS10993.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53382 Genomic DNA. Translation: CAA37462.1 .
    PIRi S10993.

    3D structure databases

    ProteinModelPortali P18134.
    SMRi P18134. Positions 1-173.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P18134.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci RETL1328306-WGS:GSTH-3881-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a gene, hpt, for hypoxanthine phosphoribosyltransferase from Vibrio harveyi."
      Showalter R.E., Silverman M.R.
      Nucleic Acids Res. 18:4621-4621(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiHPRT_VIBHA
    AccessioniPrimary (citable) accession number: P18134
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3