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Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine phosphoribosyltransferase (hpt)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Proton acceptorBy similarity1
Binding sitei130IMPBy similarity1
Binding sitei152IMP; via carbonyl oxygenBy similarity1
Metal bindingi158MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 107IMPBy similarity10
Nucleotide bindingi157 – 158IMPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HPRT
Gene namesi
Name:hpt
OrganismiVibrio harveyi (Beneckea harveyi)
Taxonomic identifieri669 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001396371 – 176Hypoxanthine phosphoribosyltransferaseAdd BLAST176

Proteomic databases

PRIDEiP18134.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi338187.VIBHAR_03458.

Structurei

3D structure databases

ProteinModelPortaliP18134.
SMRiP18134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108UGV. Bacteria.
COG0634. LUCA.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHTVEVMIS EQEVQERIRE LGKQITERYQ GSEDLVMVGL LRGSFVFMAD
60 70 80 90 100
LARAIELTHQ VDFMTASSYG NTMESSRDVR ILKDLDDDIK GKDVLIVEDI
110 120 130 140 150
IDTGNTLNKI REILSLREPK SIAICTLLDK PSRREVEVPV DYVGFAIPDE
160 170
FVVGVGIDYA QKYRDLPFIG KVVPQE
Length:176
Mass (Da):19,963
Last modified:November 1, 1990 - v1
Checksum:i2027D73A9CAAAACE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53382 Genomic DNA. Translation: CAA37462.1.
PIRiS10993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53382 Genomic DNA. Translation: CAA37462.1.
PIRiS10993.

3D structure databases

ProteinModelPortaliP18134.
SMRiP18134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi338187.VIBHAR_03458.

Proteomic databases

PRIDEiP18134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108UGV. Bacteria.
COG0634. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHPRT_VIBHA
AccessioniPrimary (citable) accession number: P18134
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.