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P18133 (PNCB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nicotinate phosphoribosyltransferase

Short name=NAPRTase
EC=6.3.4.21
Gene names
Name:pncB
Ordered Locus Names:b0931, JW0914
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP. Ref.1

Catalytic activity

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate. Ref.1

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. HAMAP-Rule MF_00570

Sequence similarities

Belongs to the NAPRTase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 400399Nicotinate phosphoribosyltransferase HAMAP-Rule MF_00570
PRO_0000205828

Sequences

Sequence LengthMass (Da)Tools
P18133 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 1D5FFC61EBAAE1D9

FASTA40045,897
        10         20         30         40         50         60 
MTQFASPVLH SLLDTDAYKL HMQQAVFHHY YDVHVAAEFR CRGDDLLGIY ADAIREQVQA 

        70         80         90        100        110        120 
MQHLRLQDDE YQWLSALPFF KADYLNWLRE FRFNPEQVTV SNDNGKLDIR LSGPWREVIL 

       130        140        150        160        170        180 
WEVPLLAVIS EMVHRYRSPQ ADVAQALDTL ESKLVDFSAL TAGLDMSRFH LMDFGTRRRF 

       190        200        210        220        230        240 
SREVQETIVK RLQQESWFVG TSNYDLARRL SLTPMGTQAH EWFQAHQQIS PDLANSQRAA 

       250        260        270        280        290        300 
LAAWLEEYPD QLGIALTDCI TMDAFLRDFG VEFASRYQGL RHDSGDPVEW GEKAIAHYEK 

       310        320        330        340        350        360 
LGIDPQSKTL VFSDNLDLRK AVELYRHFSS RVQLSFGIGT RLTCDIPQVK PLNIVIKLVE 

       370        380        390        400 
CNGKPVAKLS DSPGKTICHD KAFVRALRKA FDLPHIKKAS 

« Hide

References

« Hide 'large scale' references
[1]"Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase."
Wubbolts M.G., Terpstra P., van Beilen J.B., Kingma J., Meesters H.A.R., Witholt B.
J. Biol. Chem. 265:17665-17672(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21, FUNCTION, CATALYTIC ACTIVITY.
Strain: GEC70.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05568 Genomic DNA. Translation: AAA24400.1.
U00096 Genomic DNA. Translation: AAC74017.1.
AP009048 Genomic DNA. Translation: BAA35683.1.
PIRJQ0756.
RefSeqNP_415451.1. NC_000913.3.
YP_489203.1. NC_007779.1.

3D structure databases

ProteinModelPortalP18133.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10521N.
IntActP18133. 19 interactions.
MINTMINT-1246310.
STRING511145.b0931.

Proteomic databases

PaxDbP18133.
PRIDEP18133.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74017; AAC74017; b0931.
BAA35683; BAA35683; BAA35683.
GeneID12932887.
946648.
KEGGecj:Y75_p0903.
eco:b0931.
PATRIC32117079. VBIEscCol129921_0964.

Organism-specific databases

EchoBASEEB0735.
EcoGeneEG10742. pncB.

Phylogenomic databases

eggNOGCOG1488.
HOGENOMHOG000284928.
KOK00763.
OMAQAVFHRY.
OrthoDBEOG6X10XB.
PhylomeDBP18133.

Enzyme and pathway databases

BioCycEcoCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.
ECOL316407:JW0914-MONOMER.
MetaCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.
UniPathwayUPA00253; UER00457.

Gene expression databases

GenevestigatorP18133.

Family and domain databases

HAMAPMF_00570. NAPRTase.
InterProIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PfamPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFPIRSF000484. NAPRT. 1 hit.
SUPFAMSSF51690. SSF51690. 1 hit.
TIGRFAMsTIGR01514. NAPRTase. 1 hit.
ProtoNetSearch...

Other

PROP18133.

Entry information

Entry namePNCB_ECOLI
AccessionPrimary (citable) accession number: P18133
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene