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P18133

- PNCB_ECOLI

UniProt

P18133 - PNCB_ECOLI

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Protein

Nicotinate phosphoribosyltransferase

Gene

pncB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.1 PublicationUniRule annotation

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.1 PublicationUniRule annotation

Pathwayi

GO - Molecular functioni

  1. ligase activity, forming carbon-nitrogen bonds Source: UniProtKB
  2. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
  3. nicotinate phosphoribosyltransferase activity Source: EcoCyc

GO - Biological processi

  1. NAD biosynthetic process Source: UniProtKB
  2. nicotinate nucleotide biosynthetic process Source: InterPro
  3. response to heat Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.
ECOL316407:JW0914-MONOMER.
MetaCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.
UniPathwayiUPA00253; UER00457.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate phosphoribosyltransferaseUniRule annotation (EC:6.3.4.21UniRule annotation)
Short name:
NAPRTaseUniRule annotation
Gene namesi
Name:pncBUniRule annotation
Ordered Locus Names:b0931, JW0914
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10742. pncB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 400399Nicotinate phosphoribosyltransferasePRO_0000205828Add
BLAST

Proteomic databases

PaxDbiP18133.
PRIDEiP18133.

Expressioni

Gene expression databases

GenevestigatoriP18133.

Interactioni

Protein-protein interaction databases

DIPiDIP-10521N.
IntActiP18133. 19 interactions.
MINTiMINT-1246310.
STRINGi511145.b0931.

Structurei

3D structure databases

ProteinModelPortaliP18133.
SMRiP18133. Positions 3-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1488.
HOGENOMiHOG000284928.
InParanoidiP18133.
KOiK00763.
OMAiQAVFHRY.
OrthoDBiEOG6X10XB.
PhylomeDBiP18133.

Family and domain databases

HAMAPiMF_00570. NAPRTase.
InterProiIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR01514. NAPRTase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18133-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQFASPVLH SLLDTDAYKL HMQQAVFHHY YDVHVAAEFR CRGDDLLGIY
60 70 80 90 100
ADAIREQVQA MQHLRLQDDE YQWLSALPFF KADYLNWLRE FRFNPEQVTV
110 120 130 140 150
SNDNGKLDIR LSGPWREVIL WEVPLLAVIS EMVHRYRSPQ ADVAQALDTL
160 170 180 190 200
ESKLVDFSAL TAGLDMSRFH LMDFGTRRRF SREVQETIVK RLQQESWFVG
210 220 230 240 250
TSNYDLARRL SLTPMGTQAH EWFQAHQQIS PDLANSQRAA LAAWLEEYPD
260 270 280 290 300
QLGIALTDCI TMDAFLRDFG VEFASRYQGL RHDSGDPVEW GEKAIAHYEK
310 320 330 340 350
LGIDPQSKTL VFSDNLDLRK AVELYRHFSS RVQLSFGIGT RLTCDIPQVK
360 370 380 390 400
PLNIVIKLVE CNGKPVAKLS DSPGKTICHD KAFVRALRKA FDLPHIKKAS
Length:400
Mass (Da):45,897
Last modified:January 23, 2007 - v3
Checksum:i1D5FFC61EBAAE1D9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05568 Genomic DNA. Translation: AAA24400.1.
U00096 Genomic DNA. Translation: AAC74017.1.
AP009048 Genomic DNA. Translation: BAA35683.1.
PIRiJQ0756.
RefSeqiNP_415451.1. NC_000913.3.
YP_489203.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74017; AAC74017; b0931.
BAA35683; BAA35683; BAA35683.
GeneIDi12932887.
946648.
KEGGiecj:Y75_p0903.
eco:b0931.
PATRICi32117079. VBIEscCol129921_0964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05568 Genomic DNA. Translation: AAA24400.1 .
U00096 Genomic DNA. Translation: AAC74017.1 .
AP009048 Genomic DNA. Translation: BAA35683.1 .
PIRi JQ0756.
RefSeqi NP_415451.1. NC_000913.3.
YP_489203.1. NC_007779.1.

3D structure databases

ProteinModelPortali P18133.
SMRi P18133. Positions 3-394.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10521N.
IntActi P18133. 19 interactions.
MINTi MINT-1246310.
STRINGi 511145.b0931.

Proteomic databases

PaxDbi P18133.
PRIDEi P18133.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74017 ; AAC74017 ; b0931 .
BAA35683 ; BAA35683 ; BAA35683 .
GeneIDi 12932887.
946648.
KEGGi ecj:Y75_p0903.
eco:b0931.
PATRICi 32117079. VBIEscCol129921_0964.

Organism-specific databases

EchoBASEi EB0735.
EcoGenei EG10742. pncB.

Phylogenomic databases

eggNOGi COG1488.
HOGENOMi HOG000284928.
InParanoidi P18133.
KOi K00763.
OMAi QAVFHRY.
OrthoDBi EOG6X10XB.
PhylomeDBi P18133.

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00457 .
BioCyci EcoCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.
ECOL316407:JW0914-MONOMER.
MetaCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.

Miscellaneous databases

PROi P18133.

Gene expression databases

Genevestigatori P18133.

Family and domain databases

HAMAPi MF_00570. NAPRTase.
InterProi IPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view ]
PANTHERi PTHR11098. PTHR11098. 1 hit.
Pfami PF04095. NAPRTase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000484. NAPRT. 1 hit.
SUPFAMi SSF51690. SSF51690. 1 hit.
TIGRFAMsi TIGR01514. NAPRTase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase."
    Wubbolts M.G., Terpstra P., van Beilen J.B., Kingma J., Meesters H.A.R., Witholt B.
    J. Biol. Chem. 265:17665-17672(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21, FUNCTION, CATALYTIC ACTIVITY.
    Strain: GEC70.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPNCB_ECOLI
AccessioniPrimary (citable) accession number: P18133
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3