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P18133

- PNCB_ECOLI

UniProt

P18133 - PNCB_ECOLI

Protein

Nicotinate phosphoribosyltransferase

Gene

pncB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.1 PublicationUniRule annotation

    Catalytic activityi

    Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.1 PublicationUniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. ligase activity, forming carbon-nitrogen bonds Source: UniProtKB
    2. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
    3. nicotinate phosphoribosyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. NAD biosynthetic process Source: UniProtKB
    2. nicotinate nucleotide biosynthetic process Source: InterPro
    3. response to heat Source: EcoCyc

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Pyridine nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.
    ECOL316407:JW0914-MONOMER.
    MetaCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.
    UniPathwayiUPA00253; UER00457.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinate phosphoribosyltransferaseUniRule annotation (EC:6.3.4.21UniRule annotation)
    Short name:
    NAPRTaseUniRule annotation
    Gene namesi
    Name:pncBUniRule annotation
    Ordered Locus Names:b0931, JW0914
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10742. pncB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 400399Nicotinate phosphoribosyltransferasePRO_0000205828Add
    BLAST

    Proteomic databases

    PaxDbiP18133.
    PRIDEiP18133.

    Expressioni

    Gene expression databases

    GenevestigatoriP18133.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-10521N.
    IntActiP18133. 19 interactions.
    MINTiMINT-1246310.
    STRINGi511145.b0931.

    Structurei

    3D structure databases

    ProteinModelPortaliP18133.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAPRTase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1488.
    HOGENOMiHOG000284928.
    KOiK00763.
    OMAiQAVFHRY.
    OrthoDBiEOG6X10XB.
    PhylomeDBiP18133.

    Family and domain databases

    HAMAPiMF_00570. NAPRTase.
    InterProiIPR006406. Nic_PRibTrfase.
    IPR007229. Nic_PRibTrfase-Fam.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view]
    PANTHERiPTHR11098. PTHR11098. 1 hit.
    PfamiPF04095. NAPRTase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000484. NAPRT. 1 hit.
    SUPFAMiSSF51690. SSF51690. 1 hit.
    TIGRFAMsiTIGR01514. NAPRTase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18133-1 [UniParc]FASTAAdd to Basket

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    MTQFASPVLH SLLDTDAYKL HMQQAVFHHY YDVHVAAEFR CRGDDLLGIY    50
    ADAIREQVQA MQHLRLQDDE YQWLSALPFF KADYLNWLRE FRFNPEQVTV 100
    SNDNGKLDIR LSGPWREVIL WEVPLLAVIS EMVHRYRSPQ ADVAQALDTL 150
    ESKLVDFSAL TAGLDMSRFH LMDFGTRRRF SREVQETIVK RLQQESWFVG 200
    TSNYDLARRL SLTPMGTQAH EWFQAHQQIS PDLANSQRAA LAAWLEEYPD 250
    QLGIALTDCI TMDAFLRDFG VEFASRYQGL RHDSGDPVEW GEKAIAHYEK 300
    LGIDPQSKTL VFSDNLDLRK AVELYRHFSS RVQLSFGIGT RLTCDIPQVK 350
    PLNIVIKLVE CNGKPVAKLS DSPGKTICHD KAFVRALRKA FDLPHIKKAS 400
    Length:400
    Mass (Da):45,897
    Last modified:January 23, 2007 - v3
    Checksum:i1D5FFC61EBAAE1D9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05568 Genomic DNA. Translation: AAA24400.1.
    U00096 Genomic DNA. Translation: AAC74017.1.
    AP009048 Genomic DNA. Translation: BAA35683.1.
    PIRiJQ0756.
    RefSeqiNP_415451.1. NC_000913.3.
    YP_489203.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74017; AAC74017; b0931.
    BAA35683; BAA35683; BAA35683.
    GeneIDi12932887.
    946648.
    KEGGiecj:Y75_p0903.
    eco:b0931.
    PATRICi32117079. VBIEscCol129921_0964.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05568 Genomic DNA. Translation: AAA24400.1 .
    U00096 Genomic DNA. Translation: AAC74017.1 .
    AP009048 Genomic DNA. Translation: BAA35683.1 .
    PIRi JQ0756.
    RefSeqi NP_415451.1. NC_000913.3.
    YP_489203.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P18133.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10521N.
    IntActi P18133. 19 interactions.
    MINTi MINT-1246310.
    STRINGi 511145.b0931.

    Proteomic databases

    PaxDbi P18133.
    PRIDEi P18133.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74017 ; AAC74017 ; b0931 .
    BAA35683 ; BAA35683 ; BAA35683 .
    GeneIDi 12932887.
    946648.
    KEGGi ecj:Y75_p0903.
    eco:b0931.
    PATRICi 32117079. VBIEscCol129921_0964.

    Organism-specific databases

    EchoBASEi EB0735.
    EcoGenei EG10742. pncB.

    Phylogenomic databases

    eggNOGi COG1488.
    HOGENOMi HOG000284928.
    KOi K00763.
    OMAi QAVFHRY.
    OrthoDBi EOG6X10XB.
    PhylomeDBi P18133.

    Enzyme and pathway databases

    UniPathwayi UPA00253 ; UER00457 .
    BioCyci EcoCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.
    ECOL316407:JW0914-MONOMER.
    MetaCyc:NICOTINATEPRIBOSYLTRANS-MONOMER.

    Miscellaneous databases

    PROi P18133.

    Gene expression databases

    Genevestigatori P18133.

    Family and domain databases

    HAMAPi MF_00570. NAPRTase.
    InterProi IPR006406. Nic_PRibTrfase.
    IPR007229. Nic_PRibTrfase-Fam.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view ]
    PANTHERi PTHR11098. PTHR11098. 1 hit.
    Pfami PF04095. NAPRTase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000484. NAPRT. 1 hit.
    SUPFAMi SSF51690. SSF51690. 1 hit.
    TIGRFAMsi TIGR01514. NAPRTase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase."
      Wubbolts M.G., Terpstra P., van Beilen J.B., Kingma J., Meesters H.A.R., Witholt B.
      J. Biol. Chem. 265:17665-17672(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21, FUNCTION, CATALYTIC ACTIVITY.
      Strain: GEC70.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiPNCB_ECOLI
    AccessioniPrimary (citable) accession number: P18133
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3