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P18126

- GUNB_CELJU

UniProt

P18126 - GUNB_CELJU

Protein

Endoglucanase B

Gene

celB

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EGB is most active against barley beta-glucan, but showed significant activity against amorphous and crystalline cellulose.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei276 – 2761NucleophilePROSITE-ProRule annotation
    Active sitei393 – 3931Proton donorBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCJAP498211:GHIT-374-MONOMER.

    Protein family/group databases

    CAZyiCBM10. Carbohydrate-Binding Module Family 10.
    CBM2. Carbohydrate-Binding Module Family 2.
    GH45. Glycoside Hydrolase Family 45.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase B (EC:3.2.1.4)
    Short name:
    EGB
    Alternative name(s):
    Cellulase
    Endo-1,4-beta-glucanase
    Gene namesi
    Name:celB
    Synonyms:cel45A
    Ordered Locus Names:CJA_0374
    OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
    Taxonomic identifieri498211 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
    ProteomesiUP000001036: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 29291 PublicationAdd
    BLAST
    Chaini30 – 511482Endoglucanase BPRO_0000008023Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 127By similarity
    Disulfide bondi181 ↔ 212By similarity
    Disulfide bondi191 ↔ 206By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi498211.CJA_0374.

    Structurei

    3D structure databases

    ProteinModelPortaliP18126.
    SMRiP18126. Positions 177-224.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 130101CBM2Add
    BLAST
    Domaini180 – 20728CBM10Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi132 – 17342Ser-rich (linker)Add
    BLAST
    Compositional biasi223 – 25937Ser-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000066289.
    OMAiFANEMEN.
    OrthoDBiEOG6QCDJD.

    Family and domain databases

    Gene3Di2.30.32.30. 1 hit.
    2.40.40.10. 1 hit.
    2.60.40.290. 1 hit.
    InterProiIPR014733. Barwin-like_endoglucanase.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR002883. CBM10/Dockerin_dom.
    IPR018366. CBM2_CS.
    IPR009031. CBM_fam10.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR000334. Glyco_hydro_45.
    IPR009009. RlpA-like_DPBB.
    [Graphical view]
    PfamiPF02013. CBM_10. 1 hit.
    PF00553. CBM_2. 1 hit.
    PF02015. Glyco_hydro_45. 1 hit.
    [Graphical view]
    SMARTiSM00637. CBD_II. 1 hit.
    [Graphical view]
    SUPFAMiSSF49384. SSF49384. 1 hit.
    SSF50685. SSF50685. 1 hit.
    SSF57615. SSF57615. 1 hit.
    PROSITEiPS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS01140. GLYCOSYL_HYDROL_F45. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18126-1 [UniParc]FASTAAdd to Basket

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    MNLLSGWVRP LMLGCGLLGA ALSAGSIQAA VCEYRVTNEW GSGFTASIRI    50
    TNNGSSTING WSVSWNYTDG SRVTSSWNAG LSGANPYSAT PVGWNTSIPI 100
    GSSVEFGVQG NNGSSRAQVP AVTGAICGGQ GSSAPSSVAS SSSSSSVVSS 150
    TPRSSSSSVS SSVPGTSSSS SSSVLTGAQA CNWYGTLTPL CNNTSNGWGY 200
    EDGRSCVART TCSAQPAPYG IVSTSSSTPL SSSSSSRSSV ASSSSLSSAT 250
    SSSASSVSSV PPIDGGCNGY ATRYWDCCKP HCGWSANVPS LVSPLQSCSA 300
    NNTRLSDVSV GSSCDGGGGY MCWDKIPFAV SPTLAYGYAA TSSGDVCGRC 350
    YQLQFTGSSY NAPGDPGSAA LAGKTMIVQA TNIGYDVSGG QFDILVPGGG 400
    VGAFNACSAQ WGVSNAELGA QYGGFLAACK QQLGYNASLS QYKSCVLNRC 450
    DSVFGSRGLT QLQQGCTWFA EWFEAADNPS LKYKEVPCPA ELTTRSGMNR 500
    SILNDIRNTC P 511
    Length:511
    Mass (Da):52,078
    Last modified:November 1, 1990 - v1
    Checksum:i3C3119D998291D8E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52615 Genomic DNA. Translation: CAA36844.1.
    CP000934 Genomic DNA. Translation: ACE82688.1.
    PIRiS10527.
    RefSeqiWP_012486056.1. NC_010995.1.
    YP_001980898.1. NC_010995.1.

    Genome annotation databases

    EnsemblBacteriaiACE82688; ACE82688; CJA_0374.
    GeneIDi6416065.
    KEGGicja:CJA_0374.
    PATRICi21324125. VBICelJap122165_0383.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52615 Genomic DNA. Translation: CAA36844.1 .
    CP000934 Genomic DNA. Translation: ACE82688.1 .
    PIRi S10527.
    RefSeqi WP_012486056.1. NC_010995.1.
    YP_001980898.1. NC_010995.1.

    3D structure databases

    ProteinModelPortali P18126.
    SMRi P18126. Positions 177-224.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 498211.CJA_0374.

    Protein family/group databases

    CAZyi CBM10. Carbohydrate-Binding Module Family 10.
    CBM2. Carbohydrate-Binding Module Family 2.
    GH45. Glycoside Hydrolase Family 45.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACE82688 ; ACE82688 ; CJA_0374 .
    GeneIDi 6416065.
    KEGGi cja:CJA_0374.
    PATRICi 21324125. VBICelJap122165_0383.

    Phylogenomic databases

    HOGENOMi HOG000066289.
    OMAi FANEMEN.
    OrthoDBi EOG6QCDJD.

    Enzyme and pathway databases

    BioCyci CJAP498211:GHIT-374-MONOMER.

    Family and domain databases

    Gene3Di 2.30.32.30. 1 hit.
    2.40.40.10. 1 hit.
    2.60.40.290. 1 hit.
    InterProi IPR014733. Barwin-like_endoglucanase.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR002883. CBM10/Dockerin_dom.
    IPR018366. CBM2_CS.
    IPR009031. CBM_fam10.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR000334. Glyco_hydro_45.
    IPR009009. RlpA-like_DPBB.
    [Graphical view ]
    Pfami PF02013. CBM_10. 1 hit.
    PF00553. CBM_2. 1 hit.
    PF02015. Glyco_hydro_45. 1 hit.
    [Graphical view ]
    SMARTi SM00637. CBD_II. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49384. SSF49384. 1 hit.
    SSF50685. SSF50685. 1 hit.
    SSF57615. SSF57615. 1 hit.
    PROSITEi PS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS01140. GLYCOSYL_HYDROL_F45. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The N-terminal region of an endoglucanase from Pseudomonas fluorescens subspecies cellulosa constitutes a cellulose-binding domain that is distinct from the catalytic centre."
      Gilbert H.J., Hall J., Hazlewood G.P., Ferreira L.M.A.
      Mol. Microbiol. 4:759-767(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-48.
    2. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
      DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
      J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ueda107.

    Entry informationi

    Entry nameiGUNB_CELJU
    AccessioniPrimary (citable) accession number: P18126
    Secondary accession number(s): B3PI35
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3