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Protein

Cholesterol 7-alpha-monooxygenase

Gene

Cyp7a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.2 Publications

Catalytic activityi

Cholesterol + [reduced NADPH--hemoprotein reductase] + O2 = 7-alpha-hydroxycholesterol + [oxidized NADPH--hemoprotein reductase] + H2O.2 Publications

Cofactori

hemeBy similarity

Enzyme regulationi

Inhibition of activity by bile acid feedback.

Pathwayi: bile acid biosynthesis

This protein is involved in the pathway bile acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway bile acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi444 – 4441Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • cholesterol 7-alpha-monooxygenase activity Source: UniProtKB
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • bile acid biosynthetic process Source: UniProtKB
  • cellular response to cholesterol Source: UniProtKB
  • cellular response to glucose stimulus Source: UniProtKB
  • cholesterol catabolic process Source: UniProtKB
  • cholesterol homeostasis Source: UniProtKB
  • regulation of bile acid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14293.
BRENDAi1.14.13.17. 5301.
ReactomeiR-RNO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-RNO-211976. Endogenous sterols.
SABIO-RKP18125.
UniPathwayiUPA00221.

Chemistry

SwissLipidsiSLP:000001323.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 7-alpha-monooxygenase (EC:1.14.14.232 Publications)
Alternative name(s):
CYPVII
Cholesterol 7-alpha-hydroxylase
Cytochrome P450 7A1
Gene namesi
Name:Cyp7a1
Synonyms:Cyp7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2482. Cyp7a1.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Microsome membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2339.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Cholesterol 7-alpha-monooxygenasePRO_0000051905Add
BLAST

Proteomic databases

PaxDbiP18125.
PRIDEiP18125.

Expressioni

Tissue specificityi

Detected in liver (at protein level). Liver.2 Publications

Inductioni

Up-regulated by dietary cholesterol and cholestyramine. Down-regulated by dietary bile acids, such as chenodeoxycholic acid and cholic acid.3 Publications

Gene expression databases

BgeeiENSRNOG00000009488.
GenevisibleiP18125. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012819.

Chemistry

BindingDBiP18125.

Structurei

3D structure databases

ProteinModelPortaliP18125.
SMRiP18125. Positions 25-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiP18125.
KOiK00489.
OMAiHLQDGSY.
OrthoDBiEOG091G07UI.
PhylomeDBiP18125.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030681. Cholesterol_7a_monooxygenase.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERiPTHR24304:SF1. PTHR24304:SF1. 1 hit.
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500625. Cytochrome_CYP7A1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18125-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTISLIWGI AVLVSCCIWF IVGIRRRKAG EPPLENGLIP YLGCALKFGS
60 70 80 90 100
NPLEFLRANQ RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF
110 120 130 140 150
HYTTSAKAFG HRSIDPNDGN TTENINNTFT KTLQGDALCS LSEAMMQNLQ
160 170 180 190 200
SVMRPPGLPK SKSNAWVTEG MYAFCYRVMF EAGYLTLFGR DISKTDTQKA
210 220 230 240 250
LILNNLDNFK QFDQVFPALV AGLPIHLFKT AHKAREKLAE GLKHKNLCVR
260 270 280 290 300
DQVSELIRLR MFLNDTLSTF DDMEKAKTHL AILWASQANT IPATFWSLFQ
310 320 330 340 350
MIRSPEAMKA ASEEVSGALQ SAGQELSSGG SAIYLDQVQL NDLPVLDSII
360 370 380 390 400
KEALRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDMIALY PQLMHLDPEI
410 420 430 440 450
YPDPLTFKYD RYLDESGKAK TTFYSNGNKL KCFYMPFGSG ATICPGRLFA
460 470 480 490 500
VQEIKQFLIL MLSCFELEFV ESQVKCPPLD QSRAGLGILP PLHDIEFKYK

LKH
Length:503
Mass (Da):56,883
Last modified:November 1, 1990 - v1
Checksum:iEA825AA6E74BF5F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti371 – 3711T → S in AAA41042 (PubMed:2007596).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05509 mRNA. Translation: AAA40839.1.
J02926 Genomic DNA. Translation: AAA40923.1.
J05430 mRNA. Translation: AAA41041.1.
J05460 mRNA. Translation: AAA03649.1.
M59189
, M59184, M59185, M59186, M59187, M59188 Genomic DNA. Translation: AAA41042.1.
U01962 Genomic DNA. Translation: AAA21144.2.
X17595 mRNA. Translation: CAB57878.1.
Z14108 Genomic DNA. Translation: CAA78481.1.
PIRiA35376.
RefSeqiNP_037074.1. NM_012942.2.
UniGeneiRn.10737.

Genome annotation databases

EnsembliENSRNOT00000012819; ENSRNOP00000012819; ENSRNOG00000009488.
GeneIDi25428.
KEGGirno:25428.
UCSCiRGD:2482. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05509 mRNA. Translation: AAA40839.1.
J02926 Genomic DNA. Translation: AAA40923.1.
J05430 mRNA. Translation: AAA41041.1.
J05460 mRNA. Translation: AAA03649.1.
M59189
, M59184, M59185, M59186, M59187, M59188 Genomic DNA. Translation: AAA41042.1.
U01962 Genomic DNA. Translation: AAA21144.2.
X17595 mRNA. Translation: CAB57878.1.
Z14108 Genomic DNA. Translation: CAA78481.1.
PIRiA35376.
RefSeqiNP_037074.1. NM_012942.2.
UniGeneiRn.10737.

3D structure databases

ProteinModelPortaliP18125.
SMRiP18125. Positions 25-503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012819.

Chemistry

BindingDBiP18125.
ChEMBLiCHEMBL2339.
SwissLipidsiSLP:000001323.

Proteomic databases

PaxDbiP18125.
PRIDEiP18125.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012819; ENSRNOP00000012819; ENSRNOG00000009488.
GeneIDi25428.
KEGGirno:25428.
UCSCiRGD:2482. rat.

Organism-specific databases

CTDi1581.
RGDi2482. Cyp7a1.

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiP18125.
KOiK00489.
OMAiHLQDGSY.
OrthoDBiEOG091G07UI.
PhylomeDBiP18125.
TreeFamiTF105090.

Enzyme and pathway databases

UniPathwayiUPA00221.
BioCyciMetaCyc:MONOMER-14293.
BRENDAi1.14.13.17. 5301.
ReactomeiR-RNO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-RNO-211976. Endogenous sterols.
SABIO-RKP18125.

Miscellaneous databases

PROiP18125.

Gene expression databases

BgeeiENSRNOG00000009488.
GenevisibleiP18125. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030681. Cholesterol_7a_monooxygenase.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERiPTHR24304:SF1. PTHR24304:SF1. 1 hit.
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500625. Cytochrome_CYP7A1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP7A1_RAT
AccessioniPrimary (citable) accession number: P18125
Secondary accession number(s): P51543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.