Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P18113

- THB_RAT

UniProt

P18113 - THB_RAT

Protein

Thyroid hormone receptor beta

Gene

Thrb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei282 – 2821Thyroid hormoneBy similarity
    Binding sitei320 – 3201Thyroid hormoneBy similarity
    Binding sitei331 – 3311Thyroid hormone; via amide nitrogenBy similarity
    Binding sitei435 – 4351Thyroid hormoneBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi107 – 18175Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri107 – 12721NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri145 – 16925NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. double-stranded DNA binding Source: RGD
    2. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RGD
    3. protein homodimerization activity Source: RGD
    4. sequence-specific DNA binding Source: InterPro
    5. steroid hormone receptor activity Source: InterPro
    6. thyroid hormone binding Source: UniProtKB
    7. thyroid hormone receptor activity Source: RGD
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. embryonic organ development Source: RGD
    2. intracellular receptor signaling pathway Source: GOC
    3. positive regulation of cell proliferation Source: RGD
    4. positive regulation of chondrocyte differentiation Source: RGD
    5. positive regulation of ossification Source: RGD
    6. regulation of cholesterol metabolic process Source: RGD
    7. regulation of lipid metabolic process Source: RGD
    8. regulation of triglyceride metabolic process Source: RGD

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thyroid hormone receptor beta
    Alternative name(s):
    Nuclear receptor subfamily 1 group A member 2
    c-erbA-2
    c-erbA-beta
    Gene namesi
    Name:Thrb
    Synonyms:Erba2, Nr1a2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3858. Thrb.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: RGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461Thyroid hormone receptor betaPRO_0000053450Add
    BLAST

    Proteomic databases

    PaxDbiP18113.
    PRIDEiP18113.

    PTM databases

    PhosphoSiteiP18113.

    Expressioni

    Gene expression databases

    GenevestigatoriP18113.

    Interactioni

    Subunit structurei

    Binds DNA as a dimer; homodimer and heterodimer with RXRA By similarity. Interacts with the coactivators NCOA1/SRC1, NCOA2/GRIP1, NCOA7 and MED1/TRAP220 in a ligand-inducible manner By similarity. Interacts with the corepressor NCOR1 in absence of ligand. Interacts with C1D By similarity. Interacts with NR2F6; the interaction impairs the binding of the THRB homodimer and THRB:RXRB heterodimer to T3 response elements By similarity. Interacts with PRMT2 and THRSP By similarity.By similarity

    Structurei

    Secondary structure

    1
    461
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni108 – 1103
    Beta strandi116 – 1227
    Helixi125 – 13612
    Helixi140 – 1423
    Turni155 – 1595
    Helixi162 – 17110
    Helixi176 – 1783
    Helixi182 – 20221

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3M9EX-ray2.41A/B/E/F104-206[»]
    ProteinModelPortaliP18113.
    SMRiP18113. Positions 104-192, 211-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18113.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 106106ModulatingAdd
    BLAST
    Regioni244 – 461218Interaction with NR2F6By similarityAdd
    BLAST
    Regioni244 – 461218Ligand-bindingAdd
    BLAST

    Domaini

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri107 – 12721NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri145 – 16925NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG272726.
    HOGENOMiHOG000010313.
    HOVERGENiHBG005606.
    InParanoidiP37826.
    PhylomeDBiP18113.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001728. ThyrH_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    PR00546. THYROIDHORMR.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Beta-1 (identifier: P18113-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTPNSMTENR LPAWDKQKPH PDRGQDWKLV GMSEACLHRK SHVERRGALK    50
    NEQTSSHLIQ ATWASSIFHL DPDDVNDQSV SSAQTFQTEE KKCKGYIPSY 100
    LDKDELCVVC GDKATGYHYR CITCEGCKGF FRRTIQKSLH PSYSCKYEGK 150
    CIIDKVTRNQ CQECRFKKCI YVGMATDLVL DDSKRLAKRK LIEENREKRR 200
    REELQKSIGH KPEPTDEEWE LIKTVTEAHV ATNAQGSHWK QKRKFLPEDI 250
    GQAPIVNAPE GGQVDLEAFS HFTKIITPAI TRVVDFAKKL PMFCELPCED 300
    QIILLKGCCM EIMSLRAAVR YDPDSETLTL NGEMAVTRGQ LKNGGLGVVS 350
    DAIFDLGMSL SSFNLDDTEV ALLQAVLLMS SDRPGLACVE RIEKYQDSFL 400
    LAFEHYINYR KHHVTHFWPK LLMKVTDLRM IGACHASRFL HMKVECPTEL 450
    FPPLFLEVFE D 461
    Length:461
    Mass (Da):52,656
    Last modified:October 1, 1994 - v2
    Checksum:i16705C200E02995B
    GO
    Isoform Beta-2 (identifier: P18113-2) [UniParc] [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-93: MTPNSMTENR...QTFQTEEKKC → MNYCVPEVHE...QVRSPPHSHK

    Show »
    Length:475
    Mass (Da):54,013
    Checksum:iAA86694C55019718
    GO

    Sequence cautioni

    The sequence AAA40916.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAA42200.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 102NR → KC in AAA60743. (PubMed:2899322)Curated
    Sequence conflicti263 – 2631Q → K in AAA60743. (PubMed:2899322)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9393MTPNS…EEKKC → MNYCVPEVHEVCPAAGSNRY MQVTDYLAYLEDSPAYSGCD VQAVPGSSIYLEQAWTLNQP YTCSYPGNLFKSKDSDLDMA LSQYSQPAHLPEEKPFPQVR SPPHSHK in isoform Beta-2. 1 PublicationVSP_031079Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03933 mRNA. Translation: AAA40916.1. Different initiation.
    J03819 mRNA. Translation: AAA60743.1.
    M25071 mRNA. Translation: AAA42200.1. Different initiation.
    PIRiA31820.
    A41355.
    UniGeneiRn.34019.

    Genome annotation databases

    UCSCiRGD:3858. rat. [P18113-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03933 mRNA. Translation: AAA40916.1 . Different initiation.
    J03819 mRNA. Translation: AAA60743.1 .
    M25071 mRNA. Translation: AAA42200.1 . Different initiation.
    PIRi A31820.
    A41355.
    UniGenei Rn.34019.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3M9E X-ray 2.41 A/B/E/F 104-206 [» ]
    ProteinModelPortali P18113.
    SMRi P18113. Positions 104-192, 211-460.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P18113.
    ChEMBLi CHEMBL3917.
    GuidetoPHARMACOLOGYi 589.

    PTM databases

    PhosphoSitei P18113.

    Proteomic databases

    PaxDbi P18113.
    PRIDEi P18113.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:3858. rat. [P18113-1 ]

    Organism-specific databases

    RGDi 3858. Thrb.

    Phylogenomic databases

    eggNOGi NOG272726.
    HOGENOMi HOG000010313.
    HOVERGENi HBG005606.
    InParanoidi P37826.
    PhylomeDBi P18113.

    Miscellaneous databases

    EvolutionaryTracei P18113.
    PROi P18113.

    Gene expression databases

    Genevestigatori P18113.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001728. ThyrH_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    PR00546. THYROIDHORMR.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of rat cDNA clones for two distinct thyroid hormone receptors."
      Murray M.B., Zilz N.D., McCreary N.L., Macdonald M.J., Towle H.C.
      J. Biol. Chem. 263:12770-12777(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-1).
      Tissue: Liver.
    2. "Isolation of a cDNA clone encoding a biologically active thyroid hormone receptor."
      Koenig R.J., Warne R.L., Brent G.A., Harney J.W., Larsen P.R., Moore D.D.
      Proc. Natl. Acad. Sci. U.S.A. 85:5031-5035(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-1).
      Tissue: Pituitary.
    3. "Identification of a thyroid hormone receptor that is pituitary-specific."
      Hodin R.A., Lazar M.A., Wintman B.I., Darling D.S., Koenig R.J., Larsen P.R., Moore D.D., Chin W.W.
      Science 244:76-78(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-121 (ISOFORM BETA-2).
      Tissue: Pituitary.
    4. "Structure of a thyroid hormone receptor DNA-binding domain homodimer bound to an inverted palindrome DNA response element."
      Chen Y., Young M.A.
      Mol. Endocrinol. 24:1650-1664(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 104-206, SUBUNIT.

    Entry informationi

    Entry nameiTHB_RAT
    AccessioniPrimary (citable) accession number: P18113
    Secondary accession number(s): P37826
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3