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P18106 (FPS_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 158. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase Fps85D

EC=2.7.10.2
Alternative name(s):
dFer
Gene names
Name:Fps85D
Synonyms:Fer, fps, HD-179
ORF Names:CG8874
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1325 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Membrane; Peripheral membrane protein Ref.2.

Tissue specificity

Expressed in many tissues during embryonic development, some expression is transient and absent from most of nervous system. Larvae exhibit graded expression in all imaginal disks and expression in neural tissues. Pupal expression is seen in muscles and varies during development. Expression in adults is strong in the retina and present in ovaries, no expression is present in adult brain. Ref.1

Developmental stage

Isoforms B and D are expressed in identical patterns during all stages of development. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.

Contains 1 FCH domain.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Sequence caution

The sequence AAA93470.1 differs from that shown. Reason: Frameshift at positions 937, 941 and 944.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainSH2 domain
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Transferase
Tyrosine-protein kinase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin filament bundle assembly

Inferred from mutant phenotype PubMed 16831834. Source: FlyBase

axon guidance

Inferred from mutant phenotype PubMed 16831834. Source: FlyBase

dorsal closure

Inferred from genetic interaction PubMed 16831834. Source: FlyBase

dorsal closure, amnioserosa morphology change

Inferred from mutant phenotype PubMed 16831834. Source: FlyBase

dorsal closure, elongation of leading edge cells

Inferred from mutant phenotype PubMed 16831834. Source: FlyBase

peptidyl-tyrosine phosphorylation

Inferred from sequence or structural similarity Ref.1. Source: GOC

protein phosphorylation

Non-traceable author statement PubMed 10908587. Source: FlyBase

   Cellular_componentadherens junction

Inferred from direct assay PubMed 16831834. Source: FlyBase

extrinsic component of membrane

Inferred from direct assay Ref.2. Source: FlyBase

plasma membrane

Inferred from direct assay Ref.2. Source: FlyBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

non-membrane spanning protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phospholipid binding

Inferred from sequence model PubMed 23926047. Source: FlyBase

protein tyrosine kinase activity

Inferred from sequence or structural similarity Ref.1. Source: FlyBase

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: P18106-4)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform B (identifier: P18106-1)

Also known as: p92;

The sequence of this isoform differs from the canonical sequence as follows:
     426-946: Missing.
Isoform C (identifier: P18106-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-329: Missing.
     426-946: Missing.
Isoform D (identifier: P18106-2)

Also known as: p45;

The sequence of this isoform differs from the canonical sequence as follows:
     1-932: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13251325Tyrosine-protein kinase Fps85D
PRO_0000088095

Regions

Domain1 – 8282FCH
Domain960 – 105192SH2
Domain1063 – 1318256Protein kinase
Nucleotide binding1069 – 10779ATP By similarity

Sites

Active site11851Proton acceptor By similarity
Binding site10921ATP By similarity

Amino acid modifications

Modified residue12131Phosphotyrosine; by autocatalysis By similarity

Natural variations

Alternative sequence1 – 932932Missing in isoform D.
VSP_004986
Alternative sequence1 – 329329Missing in isoform C.
VSP_004987
Alternative sequence426 – 946521Missing in isoform B and isoform C.
VSP_004988

Experimental info

Sequence conflict581I → T in CAA37036. Ref.1
Sequence conflict230 – 24819ILQEA…KYKEI → TCRRRPSMATSRPTSTRRF in CAA37036. Ref.1
Sequence conflict320 – 34122GAVRD…NGGSP → EPSGLPGEADEDDRACEWWL A in CAA37036. Ref.1
Sequence conflict3641S → D in CAA37036. Ref.1
Sequence conflict9401V → W Ref.2
Sequence conflict10501P → T Ref.1
Sequence conflict10501P → T Ref.2
Sequence conflict11611Q → E Ref.1
Sequence conflict11611Q → E Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified March 1, 2004. Version 3.
Checksum: 7DFC98D9B9776E78

FASTA1,325150,263
        10         20         30         40         50         60 
MGFSSALQSR AAHEALIVRQ DAELRLMETM KRSIQMKAKC DKEYAISLTA VAQQGLKIDR 

        70         80         90        100        110        120 
ADEMQGSLIS KSWRSYMDEL DHQAKQFKFN AEQLEVVCDK LTHLSQDKRK ARKAYQEEHA 

       130        140        150        160        170        180 
KIAARLNHLT DEVVRKKSEY QKHLEGYKAL RTRFEENYIK APSRSGRKLD DVRDKYQKAC 

       190        200        210        220        230        240 
RKLHLTHNEY VLSITEAIEV EKDFRNVLLP GLLEHQQSVQ ESFILLWRNI LQEAAQYGDL 

       250        260        270        280        290        300 
TADKYKEIQK RIDTVIGSIN PTEEYGEFTE KYKTSPTTPL LFQFDETLIQ DIPGKLQSST 

       310        320        330        340        350        360 
LTVDNLTVDW LRNRLQELEG AVRDCQEKQM KMIEHVNGGS PVANGSIISN GSNTSNGIQS 

       370        380        390        400        410        420 
NKDSLCRQSK DLNALRCQEK QKQKLVDMIK CALNEVGCEE LPSGCDDDLT LEQNFIENGY 

       430        440        450        460        470        480 
NNEQQRSNST SSPGLGIMNE LMRRGGVLTL LRGRGRHFKR KSTPQPATPM TRSRQGRFNK 

       490        500        510        520        530        540 
LQPRSQSLGS LSVIRDGNGP SPARYEPITN HRLRQAASVH YLGEEIATSS TNPPDLTRLR 

       550        560        570        580        590        600 
RTQCSMLCLG EDEEPVVLAS PAPLTQLTAA VLTNTNNNHI YADLELDKKK DTSPSPECKG 

       610        620        630        640        650        660 
EQIQPKKEQI RIEINQTAPQ NSIDAHLDRI DELNRVLDDR LKRTLQPSDD VNAIESAEEN 

       670        680        690        700        710        720 
HIQTRKLAKD PDSQTKRSSS SSSECRSSKD TSHSKKRSLS FSQKSISNIF SNLKEFSKSP 

       730        740        750        760        770        780 
LVRMGKNHIL NEEQDAKRTQ PSQHHHSSGS DCPTNSSSSS SNNNNNNKNT SSNSNHSASQ 

       790        800        810        820        830        840 
STIITSTITT TITTTTTTTP SKENSRLKFK VPKIQKKSKA IRNTFRSKLL NFQLKRSKPC 

       850        860        870        880        890        900 
KQCTKRRRIH PSKSVFDFAK EFEVEQPAGS AADEQFCNCP PAGQKPVKPS VQISGHKDHP 

       910        920        930        940        950        960 
FESSSGELDE NSDRDIDNDE EEEDSASDDV LSMKDHCYCV PSLAASISLS TNRPLYEEEW 

       970        980        990       1000       1010       1020 
FHGVLPREEV VRLLNNDGDF LVRETIRNEE SQIVLSVCWN GHKHFIVQTT GEGNFRFEGP 

      1030       1040       1050       1060       1070       1080 
PFASIQELIM HQYHSELPVT VKSGAILRRP VCRERWELSN DDVVLLERIG RGNFGDVYKA 

      1090       1100       1110       1120       1130       1140 
KLKSTKLDVA VKTCRMTLPD EQKRKFLQEG RILKQYDHPN IVKLIGICVQ KQPIMIVMEL 

      1150       1160       1170       1180       1190       1200 
VLGGSLLTYL RKNSNGLTTR QQMGMCRDAA AGMRYLESKN CIHRDLAARN CLVDLEHSVK 

      1210       1220       1230       1240       1250       1260 
ISDFGMSREE EEYIVSDGMK QIPVKWTAPE ALNFGKYTSL CDVWSYGILM WEIFSKGDTP 

      1270       1280       1290       1300       1310       1320 
YSGMTNSRAR ERIDTGYRMP TPKSTPEEMY RLMLQCWAAD AESRPHFDEI YNVVDALILR 


LDNSH 

« Hide

Isoform B (p92) [UniParc].

Checksum: 6666EA45BF6E5784
Show »

FASTA80492,439
Isoform C [UniParc].

Checksum: 09EA965D56F33E62
Show »

FASTA47554,200
Isoform D (p45) [UniParc].

Checksum: D222275E8240C0FF
Show »

FASTA39345,192

References

« Hide 'large scale' references
[1]"A gene related to the proto-oncogene fps/fes is expressed at diverse times during the life cycle of Drosophila melanogaster."
Katzen A.L., Montarras D., Jackson J., Paulson R.F., Kornberg T., Bishop J.M.
Mol. Cell. Biol. 11:226-239(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Embryo and Head.
[2]"The DFer gene of Drosophila melanogaster encodes two membrane-associated proteins that can both transform vertebrate cells."
Paulson R.F., Jackson J., Immergluck K., Bishop J.M.
Oncogene 14:641-652(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND D), SUBCELLULAR LOCATION.
Tissue: Embryo and Head.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[5]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Head.
[6]"Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA."
Oates A.C., Wollberg P., Achen M.G., Wilks A.F.
Biochem. Biophys. Res. Commun. 249:660-667(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1191-1241.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52844 mRNA. Translation: CAA37036.1.
U50450 mRNA. Translation: AAA93470.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF54366.1.
AE014297 Genomic DNA. Translation: AAF54367.3.
AE014297 Genomic DNA. Translation: AAN13420.1.
AE014297 Genomic DNA. Translation: AAN13421.1.
BT003462 mRNA. Translation: AAO39465.1.
AJ002916 Genomic DNA. Translation: CAA05751.1.
PIROKFFPS. A39670.
RefSeqNP_524288.3. NM_079564.4. [P18106-4]
NP_731341.1. NM_169274.2. [P18106-1]
NP_731342.1. NM_169275.2. [P18106-3]
NP_731343.1. NM_169276.2. [P18106-2]
UniGeneDm.8026.

3D structure databases

ProteinModelPortalP18106.
SMRP18106. Positions 1-306, 953-1320.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid66283. 5 interactions.
IntActP18106. 4 interactions.
MINTMINT-892362.

Proteomic databases

PaxDbP18106.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0082052; FBpp0081530; FBgn0000723. [P18106-4]
GeneID41118.
KEGGdme:Dmel_CG8874.

Organism-specific databases

CTD41118.
FlyBaseFBgn0000723. Fps85D.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117264.
InParanoidP18106.
KOK08889.
OMAHIYADLE.
OrthoDBEOG708VXW.

Enzyme and pathway databases

BRENDA2.7.10.2. 1994.
SignaLinkP18106.

Gene expression databases

BgeeP18106.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR001060. FCH_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF00611. FCH. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
SMARTSM00055. FCH. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS50133. FCH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi41118.
NextBio822270.
PROP18106.

Entry information

Entry nameFPS_DROME
AccessionPrimary (citable) accession number: P18106
Secondary accession number(s): Q27576 expand/collapse secondary AC list , Q86P62, Q8INP1, Q8INP2, Q9TYI1, Q9VHE7, Q9VHE8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: March 1, 2004
Last modified: July 9, 2014
This is version 158 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase